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2010 CELL AND MOLECULAR FUNGAL BIOLOGY GORDON RESEARCH CONFERENCE, JUNE 13-18, 2010

Description: The Cellular and Molecular Fungal Biology Conference provides a forum for presentation of the latest advances in fungal research with an emphasis on filamentous fungi. This open-registration scientific meeting brings together the leading scientists from academia, government and industry to discuss current research results and future directions at Holderness School, an outstanding venue for scientific interaction. A key objective of the conference is to foster interaction among scientists working on model fungi such as Saccharomyces cerevisiae, Schizosaccharomyces pombe, Neurospora crassa and Aspergillus nidulans and scientists working on a variety of filamentous fungi whose laboratory tractability is often inversely proportional to their medical, industrial or ecological importance. Sessions will be devoted to Systems Biology, Fungi and Cellulosic Biomass, Small RNAs, Population Genomics, Symbioses, Pathogenesis, Membrane Trafficking and Polarity, and Cytoskeleton and Motors. A session will also be devoted to hot topics picked from abstracts. The CMFB conference provides a unique opportunity to examine the breadth of fungal biology in a small meeting format that encourages in-depth discussion among the attendees.
Date: June 18, 2010
Creator: Momany, Michelle
Partner: UNT Libraries Government Documents Department

Large scale solubilization of coal and bioconversion to utilizable energy. Quarterly technical progress report, January-March 1994

Description: In order to develop a system for large scale coal solubilization and its bioconversion to utilizable fuel, the authors plan to clone the genes encoding Neurospora protein that facilitate depolymerization of coal. They also plan to use desulfurizing bacteria to remove the sulfur in situ and use other microorganisms to convert biosolubilized coal into utilizable energy following an approach utilizing several microorganisms (Faison). In addition the product of coal solubilized by fungus will be characterized to determine their chemical nature and the mechanism of reaction catalyzed by fungal product during in vivo and in vitro solubilization by the fungus or purified fungal protein.
Date: June 1, 1994
Creator: Mishra, N. C.
Partner: UNT Libraries Government Documents Department

Large scale solubilization of coal and bioconversion to utilizable energy. Quarterly report, July 1, 1996--September 30 1996

Description: A purification of the Neurospora protein with coal solubilization activity (CSA) using DEAE cellulose chromatography is described. The protein is heavily glycosylated suggesting that it is different than tyrosinase or common phenol oxidases even though it resembles these proteins in enzyme activity and molecular weight.
Date: December 31, 1996
Creator: Mishra, N.C.
Partner: UNT Libraries Government Documents Department

Large scale solubilization of coal and bioconversion to utilizable energy. Technical progress report, January 1--March 31, 1996

Description: In order develop a system for a large scale coal solubilization and its bioconversion to utilizable fuel, the authors plan to clone the genes encoding Neurospora protein that facilitates depolymerization of coal. They also plan to use desulfurizing bacteria to remove the sulfur in situ and use other microorganisms to convert biosolubilized coal into utilizable energy following an approach utilizing several microorganisms. In addition the products of coal solubilized by fungus will be characterized to determine their chemical nature and the mechanism of reaction catalyzed by fungal product during in vivo and in vitro solubilization by the fungus or purified fungal protein. Results are presented for the cloning of genes for Neurospora CSA-protein.
Date: May 1, 1996
Creator: Mishra, N.C.
Partner: UNT Libraries Government Documents Department

Large scale solubilization of coal and bioconversion to utilizable energy. Seventh quarterly technical progress report, April 1, 1995--June 30, 1995

Description: In order to develop a system for a large scale coal solubilization and its bioconversion to utilizable fuel, we plan to clone the genes encoding Neurospora protein that facilitate depolymerization of coal. We also plan to use desulfurizing bacteria to remove the sulfur in situ and use other microorganisms to convert biosolubilized coal into utilizable energy following an approach utilizing several microorganisms. In addition the product of coal solubilized by fungus will be characterized to determine their chemical nature and the mechanism of reaction catalyzed by fungal product during in vivo and in vitro solubilization by the fungus or purified fungal protein.
Date: December 1, 1995
Creator: Mishra, N.C.
Partner: UNT Libraries Government Documents Department

Light responses in Photoperiodism in Arabidopsis thaliana

Description: ADO1: An Arabidopsis blue light photoreceptor We have reported the characterization of an Arabidopsis gene encoding the ADAGIO 1 (ADO1) protein (Jarillo et al., 2001a). ADO1 contains a LOV domain, similar to WHITE COLLAR 1 (WC1), a photoreceptor for entrainment of Neurospora circadian rhythms (Froehlich et al., 2002), as well as PHOT1 and PHOT2, the blue light photoreceptors for phototropism (Briggs et al., 2001; Christie et al., 1998; Jarillo et al., 2001b; Kinoshita et al., 2001). Loss of function ado1 mutants show an unusually long periodicity for their free running circadian rhythm (Jarillo et al., 2001a). This observation holds for plants grown under white light as well as blue light and surprisingly, plants grown under red light also show altered circadian properties. The similarity of the LOV domain of ADO1 to those of PHOT1, PHOT2 and WC1 (known flavoprotein photoreceptors) as well as the genetic and molecular properties of ADO1, indicate that ADO1 is likely a new class of blue light photoreceptor. Indeed, the LOV domain of the related FKF1/ADO3 has been shown to bind FMN, and exhibit the in vitro photochemistry characteristic of PHOT1 (Imaizumi et al., 2003). Furthermore, ZTL/ADO1 has been shown to participate in the circadian and proteasome mediated degradation of the Arabidopsis clock protein, TOC1 (Mas et al., 2003). We also showed that the ado1 mutation selectively confers hypersensitivity to red light — when grown under red light (but not blue light) the ado1 mutant possesses an unusually short hypocotyl. This red light hypersensivity is even more severe in a triple ado1 ado2 ado3 mutant — ADO2 and ADO3 being the two other members of this ADAGIO gene family. This finding of a mutant phenotype under red light is somewhat unexpected for a protein thought to function as a photoreceptor for blue light. We have pursued ...
Date: August 1, 2006
Creator: Cashmore, Anthony R.
Partner: UNT Libraries Government Documents Department

Ultraviolet-inactivation of conidia from heterokaryons of Neurospora crassa containing uv-sensitive mutations

Description: From 7th neurospora information conference; Asilomar, California, USA (25 Mar 1974). The effect of three uv-sensitive mutations of Neurospora crassa, upr-1, uvs-4 and uvs-6, on the ultraviolet-inactivation of conidia from two- component heterokaryons was investigated. In twocomponent heterokaryons with wild-type sensitivity to radiation inactivation, all three conidial fractions exhibited similar ultraviolet-inactivation curves. Each uv-sensitive mutation studied uniquely modified the ultraviolet-inactivation curves of conidia from two- component heterokaryons. In heterokaryons heterokaryotic for upr-1, the upr-1 mutation was recessive and the repair function determined by the wild-type allele was functional to some degree in homokaryotic upr-1 conidia. All three conidial fractions of heterokaryons containing upr-1 in both components showed increased sensitivity to ultraviolet light. The uvs-4 mutation was recessive and resulted in conidia with increased uv-sensitivity only when included in both components of a heterokaryon. Homokaryotic uvs-4 conidia, which arose from heterokaryons containing both uvs-4 and wild-type components, exhibited wild-type survival. Therefore, as with upr-1, there was a carryover of the repair capability to conidia which were genetically uv-sensitive. The uvs-6 mutation, when included in one component of a two-component heterokaryon, resulted in increased uv- sensitivity of both heterokaryotic and homokaryotic uvs-6 conidia. When both components contained uvs-6, the uv-sensitivity of all three conidial fractions was increased and all showed similar inactivation curves. Thus, as with upr-1 and uvs-4, there was a carryover of the wildtype repair capability to geneticaily uvs-6 conidia. Heterokaryon tests for complementation between two non-allelic uv- sensitive mutations showed that in heterokaryotic conidia, complete complementation occurred between upr-1 and uvs-4. (auth)
Date: January 1, 1973
Creator: Shelby, M.D.; de Serres, F.J. & Stine, G.J.
Partner: UNT Libraries Government Documents Department

Large scale solubilization of coal and bioconversion to utilizable energy. Quarterly report, October 1, 1995--December 31, 1995

Description: The ability of Neurospora to solubilize and bioconvert coal was investigated. The coal solubilizing activity (CSA) was fractionated to isolate the enzyme responsible for this activity. The enzyme was purified in order to obtain the amino acid sequence. From that sequence potential oligonucleotide probes were synthesized and used to screen genomic library of Neurospora. The gene so identified was isolated. CSA appears to be an phenol oxidase or is tyrosinase.
Date: December 31, 1995
Creator: Mishra, N.C.
Partner: UNT Libraries Government Documents Department

Large scale solubilization of coal and bioconversion to utilizable energy. Eleventh quarterly technical progress report, April 1, 1996--June 30, 1996

Description: Neurospora has the capability to solubilize coal and the protein fraction accounting for this ability has been isolated. During this period the cola solubilizing activity (CSA) was fractionated and partially sequenced. The activity has been determined to be a tyrosinase and/or a phenol oxidase. The amino acid sequence of the protein was used to prepare oligonucleotides to identify the clone carrying Neurospora CSA. It is intended to clone the Neurospora gene into yeast, since yeast cannot solubilize coal, to further characterize the CSA.
Date: October 1, 1996
Creator: Mishra, N.C.
Partner: UNT Libraries Government Documents Department

Large scale solubilization of coal and bioconversion to utilizable energy. Eighth quarterly technical progress report, July 1, 1995--September 30, 1995

Description: In order to develop a system for a large scale coal solubilization and its bioconversion to utilizable fuel, we plan to clone the genes encoding Neurospora protein that facilitate depolymerization of coal. We also plan to use desulfurizing bacteria to remove the sulfur in situ and use other microorganisms to convert biosolubilized coal into utilizable energy following an approach utilizing several microorganisms. In addition the product of coal solubilized by fungus will be characterized to determine their chemical nature and the mechanism of reaction catalyzed by fungal product during in vivo and in vitro solubilization by the fungus or purified fungal protein.
Date: February 1, 1996
Creator: Mishra, N.C.
Partner: UNT Libraries Government Documents Department

Large scale solubilization of coal and bioconversion to utilizable energy. Fifth quarterly technical report, January 1, 1995--March 31, 1995

Description: In order to develop a system for a large scale coal solubilization and its bioconversion to utilizable fuel, we plan to clone the genes encoding Neurospora protein that facilitate depolymerization of coal. We also plan to use desulfurizing bacteria to remove the sulfur in situ and use other microorganisms to convert biosolubilized coal into utilizable energy following an approach utilizing several microorganisms. In addition the product of coal solubilized by fungus will be characterized to determine their chemical nature and the mechanism of reaction catalyzed by fungal product during in vivo and in vitro solubilization by the fungus or purified fungal protein.
Date: December 1, 1995
Creator: Mishra, N.C.
Partner: UNT Libraries Government Documents Department

Large scale solubilization of coal and bioconversion to utilizable energy. Third quarterly technical progress report, April 1, 1994--June 30, 1994

Description: In order to develop a system for a large scale coal solubilization and its bioconversion to utilizable fuel, the investigators plan to clone the genes encoding Neurospora protein that facilitate depolymerization of coal. They also plan to use desulfurizing bacteria to remove the sulfur in situ and use other microorganisms to convert biosolubilized coal into utilizable energy following an approach utilizing several microorganisms. In addition the product of coal solubilized by fungus will be characterized to determine their chemical nature and the mechanism of reaction catalyzed by fungal product during in vivo and in vitro solubilization by the fungus or purified fungal protein. Main objectives are: (1) cloning of Neurospora gene for coal depolymerization protein controlling solubilization in different host cells, utilizing Neurospora plasmid and other vector(s); (2) (a) development of a large scale electrophoretic separation of coal drived products obtained after microbial solubilization; (b) identification of the coal derived products obtained after biosolubilization by Neurospora cultures or obtained after Neurospora enzyme catalyzed reaction in in vitro by the wildtype and mutant enzymes; (3) bioconversion of coal drived products into utilizable fuel; and (4) characterization of Neurospora wildtype and mutant CSA protein(s) involved in solubilization of coal in order to assess the nature of the mechanism of solubilization and the role of Neurospora proteins in this process.
Date: August 1, 1994
Creator: Mishra, N. C.
Partner: UNT Libraries Government Documents Department

Differential gene expression in neurospora crassa cell types: amplification of rRNA genes. Progress report, July 1979-30 June 1980

Description: The significant results obtained during 1979 to 1980 of the current research program are as follows: (1) the differential rRNA gene amplification in germinated conidia of N.crassa was confirmed. N.crassa rDNAs showed differences in degrees of homology with isolated DNAs from other Neurospora species which could be due to heterogeneity in internal spacers. Studies with N.crassa rDNA clones were initiated to study their heterogeneities. The organization of the Institutional Biohazard Committee (IBC) for Recombinant DNA research was completed and necessary certifications for the laboratory and the workers were obtained in accordance with the P/sub 2/EK/sub 1/ containment regulation of N.I.H. Known 17S and 26S N.crassa rDNA probes are being used to detect differences, if any, in restriction cleavage sites in rDNAs of different cell types and developmental mutants of N.crassa. DNAs from these N.crassa cells are restricted with EcoR/sub 1/ and Hind III and cleaved fragments separated by gel electrophoresis are transferred into nitrocellulose papers. Experiments are underway now to see if there are any changes in cleavage sites by annealing with /sup 32/P or /sup 3/H-17S or 26S rDNA probes followed by autoradiography.
Date: January 1, 1980
Creator: Dutta, S.K.
Partner: UNT Libraries Government Documents Department

Gene enzyme relationships in somatic cells and their organismal derivatives in higher plants. Progress report

Description: This renewal proposal describes preliminary results on the isolation and characterization of a new amino acid, spiro-arogenate. The preparation of /sup 14/C-L-arogenate and an improved assay for arogenate dehydratase are described. The remainder of the proposal deals with the cell biology of Nicotiana. (KRM)
Date: January 1, 1981
Creator: Jensen, R.A.
Partner: UNT Libraries Government Documents Department

Comparative mutagenesis. Progress report, January 1, 1977-December 31, 1977 (extended from January 1, 1978-December 31,1978

Description: Our major research efforts have been on the mutagenicity of certain ICR-compounds and on studies in environmental mutagenesis. From a comparison of the mutagenicity of 16 ICR-compounds, we concluded that Neurospora crassa is a better predictor than Salmonella typhimurium of antitumor and carcinogenic potencies in mice. Streptonigrin induced a high frequency (25%) of multilocus deletions at the ad-3 region of N. crassa. Most of the water concentrates prepared from Lake Bloomington, IL were weakly mutagenic in the Salmonella/microsome test; one concentrate was highly mutagenic in strain TA100. Chemical analysis of the highly mutagenic concentrate revealed the presence of a number of organic compounds that were absent or very low in the weakly mutagenic concentrates. Cigarette smoke condensate and certain factions of the condensate were mutagenic in N. crassa, Saccharomyces cerevisiae, and Drosophila melanogaster. In D. melanogaster, cigarette smoke also was mutagenic. In N. crassa, cigarette smoke condensate appeared to be direct acting. Sodium azide was not mutagenic in N. crassa at pH values of 3 to 8, and there were no significant differences in the induced reversion frequencies over this pH range in S. typhimurium.
Date: December 1, 1978
Creator: Brockman, H. E.
Partner: UNT Libraries Government Documents Department