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[Four boys sample molasses]

Description: Photograph of four boys sampling molasses with sop sticks at a molasses stir-off in Cumberland Gap, Tennessee. Narrative by Junebug Clark: Additional photos and information can be found in the pdf document "Joe Clark HBSS LIFE Magazine Photos" page 20. November 13, 1950. Life Magazine pages 156-160. Stir-off Time in Tennessee. Fun Starts in Hills as Molasses Boils. Alos in Joe's book "I Remember" Photo by: Joe Clark, HBSS. Signed by: Joe Clark, HBSS Clark PhotoFile: 2-31
Date: 1950
Creator: Clark, Joe
Partner: UNT Libraries Special Collections

[Hoe Down at the Molasses Stir-off]

Description: Photograph of a large group of friends and neighbors dancing during the Hoe Down event following the molasses stir-off. Narrative by Junebug Clark: Additional photos and information can be found in the pdf document "Joe Clark HBSS LIFE Magazine Photos" page 20. Also, November 13, 1950. Life Magazine pages 156-160. Stir-off Time in Tennessee. Fun Starts in Hills as Molasses Boils. People would come from miles around to participate in the Molasses Making Stir-off every year. The stir-off was held either on the farm of Fred Whitaker about four miles southwest of Cumberland Gap, or on the farm of Baptist preacher the Rev. Hugh Vancel in Cumberland Gap proper. Photo by: Joe Clark, HBSS.
Date: 194u
Creator: Clark, Joe
Partner: UNT Libraries Special Collections

A low-energy continuous reactor-separator for ethanol from starch, whey permeate, permeate mother liquor, molasses or cellulosics. Project final report, April 1, 1994--February 28, 1997

Description: In this project, a novel bio-reactor technology in which reaction is coupled with product separation was developed to pilot/demonstration scale. Combining reaction with separation during a fermentation allows the fermentation of highly concentrated feeds and allows the fermentation of streams with high levels of salts/non-fermentable inhibitors. Simultaneous saccharification and fermentation of polysaccharides such as starch and cellulose can also be combined with ethanol separation in the Continuous Stirred Reactor Separator (CSRS). Application of the bio-reactor to various substrates was investigated on a lab scale with fermentation of raw starch, cane molasses, xylose, whey permeate and permeate mother liquor. Flocculating yeast strains for high density sucrose/glucose fermentation were selected and adapted to form fast settling pellets. A strain of K marxianus capable of fermenting high salt permeate mother liquor was also selected and adapted. A low energy solvent ethanol recovery system was developed for ethanol recovery from the vapors leaving the reactor/separator. This Solvent Absorption/Extractive Distillation (SAED) process gives a low energy method for purifying the ethanol to an anhydrous product. The amount of energy needed to recover an anhydrous ethanol product from a CSRS stage running at 8% ethanol was calculated to be under 8,000 BTU/gallon. This process may also have further application in VOC (volatile organic carbon compounds) removal from air streams. During this project, a 24,000 Liter CSRS was designed, fabricated, installed, and operated at a small batch ethanol plant (Permeate Refining Inc) in NE Iowa. The reactor was operated on a semi-continuous basis over a period of 18 months. A Solvent Absorption Extractive Distillation (SAED) system was also recently completed and installed at the Permeate Refining Inc. site for ethanol recovery/dehydration.
Date: April 14, 1997
Creator: Dale, M.C. & Moelhman, M.
Partner: UNT Libraries Government Documents Department

Effects of Varying RedoxConditions on Natural Attenuation of Inorganic Contaminants from the D-Area Coal Pile Runoff Basin (U)

Description: The objective of this study was to provide geochemical parameters to characterize the D-Area Coal Pile Runoff Basin (DCPRB) sediment as a potential source term. It is anticipated that the measured values will be used in risk calculations and will provide additional technical support for imposing Monitored Natural Attenuation at D-Area. This study provides a detailed evaluation of the DCPRB sediment and is part of another study that quantified the Monitored Natural Attenuation of inorganic contaminants more broadly at the D-Area Expanded Operable Unit, which includes the DCPRB (Powell et al. 2004). Distribution coefficients (K{sub d} values; a solid to liquid contaminant concentration ratio) and the Potentially Leachable Fraction (the percent of the total contaminant concentration in the sediment that can likely contribute to a contaminant plume) were measured in a DCPRB sediment as a function of redox conditions. Redox conditions at the DCPRB are expected to vary greatly as the system undergoes varying drying and flooding conditions. Conservative values; K{sub d} values that err on the side of being too low and Potentially Leachable Fraction values that err on the side of being too high, are presented. The K{sub d} values are high compared to conservative literature values, and underscores the importance of measuring site-specific values to provide estimates of sediments natural attenuation/sorption capacities. The Potentially Leachable Fraction indicates that as little as 27% of the As, but all of the Cu and Tl will be part of the source term. In the case of the As, the remaining 83% will likely never leach out of the sediment, thereby providing a form of natural attenuation. Importantly, Be, Cr, Cu, Ni, and V concentrations in the sediment were less-than twice background levels, indicating this sediment was not a potential source for these contaminants. K{sub d} values generally increased significantly (As, ...
Date: May 30, 2004
Creator: Kaplan, D
Partner: UNT Libraries Government Documents Department

Differential sensitivity of aerobic gram-positive and gram-negative microorganisms to 2,4,6-trinitrotoluene (TNT) leads to dissimilar growth and TNT transformation: Results of soil and pure culture studies

Description: The effects of 2,4,6-trinitrotoluene (TNT) on indigenous soil populations and pure bacterial cultures were examined. The number of colony-forming units (CFU) appearing when TNT-contaminated soil was spread on 0.3% molasses plates decreased by 50% when the agar was amended with 67 {mu}g TNT mL{sup -1}, whereas a 99% reduction was observed when uncontaminated soil was plated. Furthermore, TNT-contaminated soil harbored a greater number of organisms able to grow on plates amended with greater than 10 {mu}g TNT mL{sup -1}. The percentage of gram-positive isolates was markedly less in TNT-contaminated soil (7%; 2 of 30) than in uncontaminated soil (61%; 20 of 33). Pseudomonas aeruginosa, Pseudomonas corrugate, Pseudomonasfluorescens and Alcaligenes xylosoxidans made up the majority of the gram-negative isolates from TNT-contaminated soil. Gram-positive isolates from both soils demonstrated marked growth inhibition when greater than 8-16 {mu}g TNT mL{sup -1} was present in the culture media. Most pure cultures of known aerobic gram-negative organisms readily degraded TNT and evidenced net consumption of reduced metabolites. However, pure cultures of aerobic gram-positive bacteria were sensitive to relatively low concentrations of TNT as indicated by the 50% reduction in growth and TNT transformation which was observed at approximately 10 {mu}g TNT mL{sup -1}. Most non-sporeforming gram-positive organisms incubated in molasses media amended with 80 {mu}g TNT mL{sup -1} or greater became unculturable, whereas all strains tested remained culturable when incubated in mineral media amended with 98 {mu}g TNT mL{sup -1}, indicating that TNT sensitivity is likely linked to cell growth. These results indicate that gram-negative organisms are most likely responsible for any TNT transformation in contaminated soil, due to their relative insensitivity to high TNT concentrations and their ability to transform TNT.
Date: July 30, 1996
Creator: Fuller, M.E. & Manning, J.F. Jr.
Partner: UNT Libraries Government Documents Department

Experiment Station Work, [Volume] 13

Description: Bulletin issued by the U. S. Department of Agriculture compiling selected articles from the Agricultural Experiment Stations. This bulletin contains articles on: Fertilizer Requirements of Crops, Persimmons, Forcing Rhubarb, Grinding Corn for Cows, Waste in Feeding Cornstalks, Molasses for Farm Animals, Feeding Ducks, Cost of Raising Calves, Feeding Calves with Milk of Tuberculous Cows, Killing the Germs of Tuberculosis in Milk, Ropy Milk and Cream, Dairy Salt.
Date: 1899
Creator: United States. Office of Experiment Stations.
Partner: UNT Libraries Government Documents Department


Description: The objective of this NETL sponsored bench-scale study was to demonstrate the efficacy of enhanced anaerobic reductive precipitation (EARP) technology for precipitating uranium using samples from contaminated groundwater at the Fernald Closure Project (FCP) in Cincinnati, Ohio. EARP enhances the natural biological reactions in the groundwater through addition of food grade substrates (typically molasses) to drive the oxidative-reductive potential of the groundwater to a lower, more reduced state, thereby precipitating uranium from solution. In order for this in-situ technology to be successful in the long term, the precipitated uranium must not be re-dissolved at an unacceptable rate once groundwater geochemical conditions return to their pretreatment, aerobic state. The approach for this study is based on the premise that redissolution of precipitated uranium will be slowed by several mechanisms including the presence of iron sulfide precipitates and coatings, and sorption onto fresh iron oxides. A bench-scale study of the technology was performed using columns packed with site soil and subjected to a continuous flow of uranium-contaminated site groundwater (476 {micro}g/L). The ''treated'' column received a steady stream of dilute food grade molasses injected into the contaminated influent. Upon attainment of a consistently reducing environment and demonstrated removal of uranium, an iron sulfate amendment was added along with the molasses in the influent solution. After a month long period of iron addition, the treatments were halted, and uncontaminated, aerobic, unamended water was introduced to the treated column to assess rebound of uranium concentrations. In the first two months of treatment, the uranium concentration in the treated column decreased to the clean-up level (30 {micro}g/L) or below, and remained there for the remainder of the treatment period. A brief period of resolubilization of uranium was observed as the treated column returned to aerobic conditions, but the concentration later returned to below the clean-up ...
Date: August 1, 2003
Creator: Lutes, Christopher C.; Angela Frizzell, PG; Thornton, Todd A. & Harrington, James M.
Partner: UNT Libraries Government Documents Department

Poisoning the Cotton Boll Weevil.

Description: A guide to selecting the most suitable method for controlling the boll weevil. Describes poisons and equipment used in boll-weevil control.
Date: April 1929
Creator: Coad, B. R. & Gaines, R. C. (Robert Callaway), 1898-
Partner: UNT Libraries Government Documents Department

[Arriving at the Stir-off]

Description: Narrative by Junebug Clark in the summer of 2014 Friends and neighbors come from miles around to participate in the Molasses Making Stir-off. Overall Background: These Molasses Making Stir-off photos were shot by Joe Clark HBSS in the early to mid-1940s. Either on the farm of Fred Whitaker about four miles southwest of Cumberland Gap, or in Cumberland Gap on the farm of Baptist preacher the Rev. Hugh Vancel. More information about these images can be found in scrapbooks in the Clark Family Collection at the University of North Texas Special Collections Library. Specifically in a Detroit news pictorial article published December 13, 1942 titles "stir-off party" where mountaineers make molasses and merriment. Also in life magazine published November 13, 1950 [page 156] titled “Stir-off Time in Tennessee, Fun Starts in Hills as Molasses Boils.’ Also in the library is the NBC Today Show story on Joe Clark HBSS by Bob Dotson. It features Joe Clark returning to Cumberland Gap Tennessee to photograph a molasses stir off taking place in the same location as some of these photographs and attended by some of the same people in the early 1980s. Let me briefly set the scene of the time and era that these photos were made. World War II was raging. Television did not exist. Radio reception sporadic in this mountain country. School was held only three months a year mostly during the winter months. Most news of the changes in the outside world was learned, they say, “By looking at pictures in the Sears catalogs.” They farmed steep and rolling hillsides of very poor land. In most of America, to help in the war effort, the rationing of food and other essentials was prevalent. Very few of these Tennessee mountaineers and hill folk had homes with electricity. None with ...
Date: 194u
Creator: Clark, Joe
Partner: UNT Libraries Special Collections

Agriculture: Bioconversion of sugar cane molasses

Description: Auxein Corporation is demonstrating for commercial use an organic acid phytochelate, derived from what would otherwise be a discarded portion of sugar cane, that could increase the domestic sugar industry's profit margin from near zero to 7%. Along with helping a struggling industry, the phytochelate will bring substantial improvements to crop and tree production and greatly reduce the environmental threat posed by nitrogen-based fertilizers. Currently, the amount of fertilizer used produces harmful levels of run-off that contaminates ground water with unwanted nitrogen. By utilizing organic acid phytochelates, which assist plant growth by unlocking minerals stored in soil, fertilizer use can be dramatically reduced. This would improve crop yields, remove environmental threats to ground water, and cut fertilizer costs by as much as 50%.
Date: September 29, 1999
Creator: Simon, P.
Partner: UNT Libraries Government Documents Department

Experiment Station Work, [Volume] 36

Description: Bulletin issued by the U. S. Department of Agriculture compiling selected articles from the Agricultural Experiment Stations. This bulletin contains articles on: Water for Table Use, Phosphates, Winter Wheat, Glutenous and Starchy Wheats, Dry Farming, Methods of Canning, Beet Molasses and Pulp, Feed Lots, Guinea Fowls, Color of Eggs, Spraying for Scale Insects, and White Pine in New England.
Date: 1906
Creator: United States. Office of Experiment Stations.
Partner: UNT Libraries Government Documents Department

Vadose zone microbial community structure and activity in metal/radionuclide contaminated sediments. Final technical report

Description: This final technical report describes the research carried out during the final two months of the no-cost extension ending 11/14/01. The primary goals of the project were (1) to determine the potential for transformation of Cr(VI) (oxidized, mobile) to Cr(III) (reduced, immobile) under unsaturated conditions as a function of different levels and combinations of (a) chromium, (b) nitrate (co-disposed with Cr), and (c) molasses (inexpensive bioremediation substrate), and (2) to determine population structure and activity in experimental treatments by characterization of the microbial community by signature biomarker analysis and by RT-PCR and terminal restriction fragment length polymorphism (T-RFLP) and 16S ribosomal RNA genes. It was determined early in the one-year no-cost extension period that the T-RFLP approach was problematic in regard to providing information on the identities of microorganisms in the samples examined. As a result, it could not provide the detailed information on microbial community structure that was needed to assess the effects of treatments with chromium, nitrate, and/or molasses. Therefore, we decided to obtain the desired information by amplifying (using TR-PCR, with the same primers used for T-RFLP) and cloning 16S rRNA gene sequences from the same RNA extracts that were used for T-RFLP analysis. We also decided to use a restriction enzyme digest procedure (fingerprinting procedure) to place the clones into types. The primary focus of the research carried out during this report period was twofold: (a) to complete the sequencing of the clones, and (b) to analyze the clone sequences phylogenetically in order to determine the relatedness of the bacteria detected in the samples to each other and to previously described genera and species.
Date: August 17, 2002
Creator: Balkwill, David L.
Partner: UNT Libraries Government Documents Department

[News Clip: Molasses]

Description: B-roll video footage from the KXAS-TV/NBC station in Fort Worth, Texas, to accompany a news story.
Date: August 16, 1979, 6:00 p.m.
Creator: KXAS-TV (Television station : Fort Worth, Tex.)
Partner: UNT Libraries Special Collections


Description: The Audubon Sugar Institute (ASI) of Louisiana State University’s Agricultural Center (LSU AgCenter) and MBI International (MBI) sought to develop technologies that will lead to the development of a sugar-cane biorefinery, capable of supplying fuel ethanol from bagasse. Technology development focused on the conversion of bagasse, cane-leaf matter (CLM) and molasses into high value-added products that included ethanol, specialty chemicals, biomaterials and animal feed; i.e. a sugar cane-based biorefinery. The key to lignocellulosic biomass utilization is an economically feasible method (pretreatment) for separating the cellulose and the hemicellulose from the physical protection provided by lignin. An effective pretreatment disrupts physical barriers, cellulose crystallinity, and the association of lignin and hemicellulose with cellulose so that hydrolytic enzymes can access the biomass macrostructure (Teymouri et al. 2004, Laureano-Perez, 2005). We chose to focus on alkaline pretreatment methods for, and in particular, the Ammonia Fiber Expansion (AFEX) process owned by MBI. During the first two years of this program a laboratory process was established for the pretreatment of bagasse and CLM using the AFEX process. There was significant improvement of both rate and yield of glucose and xylose upon enzymatic hydrolysis of AFEX-treated bagasse and CLM compared with untreated material. Because of reactor size limitation, several other alkaline pretreatment methods were also co-investigated. They included, dilute ammonia, lime and hydroxy-hypochlorite treatments. Scale-up focused on using a dilute ammonia process as a substitute for AFEX, allowing development at a larger scale. The pretreatment of bagasse by an ammonia process, followed by saccharification and fermentation produced ethanol from bagasse. Simultaneous saccharification and fermentation (SSF) allowed two operations in the same vessel. The addition of sugarcane molasses to the hydrolysate/fermentation process yielded improvements beyond what was expected solely from the addition of sugar. In order to expand the economic potential for building a biorefinery, the conversion ...
Date: January 29, 2009
Creator: Day, Dr. Donal F.
Partner: UNT Libraries Government Documents Department

Separation of Corn Fiber and Conversion to Fuels and Chemicals Phase II: Pilot-scale Operation

Description: The purpose of the Department of Energy (DOE)-supported corn fiber conversion project, “Separation of Corn Fiber and Conversion to Fuels and Chemicals Phase II: Pilot-scale Operation” is to develop and demonstrate an integrated, economical process for the separation of corn fiber into its principal components to produce higher value-added fuel (ethanol and biodiesel), nutraceuticals (phytosterols), chemicals (polyols), and animal feed (corn fiber molasses). This project has successfully demonstrated the corn fiber conversion process on the pilot scale, and ensured that the process will integrate well into existing ADM corn wet-mills. This process involves hydrolyzing the corn fiber to solubilize 50% of the corn fiber as oligosaccharides and soluble protein. The solubilized fiber is removed and the remaining fiber residue is solvent extracted to remove the corn fiber oil, which contains valuable phytosterols. The extracted oil is refined to separate the phytosterols and the remaining oil is converted to biodiesel. The de-oiled fiber is enzymatically hydrolyzed and remixed with the soluble oligosaccharides in a fermentation vessel where it is fermented by a recombinant yeast, which is capable of fermenting the glucose and xylose to produce ethanol. The fermentation broth is distilled to remove the ethanol. The stillage is centrifuged to separate the yeast cell mass from the soluble components. The yeast cell mass is sold as a high-protein yeast cream and the remaining sugars in the stillage can be purified to produce a feedstock for catalytic conversion of the sugars to polyols (mainly ethylene glycol and propylene glycol) if desirable. The remaining materials from the purification step and any materials remaining after catalytic conversion are concentrated and sold as a corn fiber molasses. Additional high-value products are being investigated for the use of the corn fiber as a dietary fiber sources.
Date: September 28, 2007
Creator: Abbas, Charles; Beery, Kyle; Orth, Rick & Zacher, Alan
Partner: UNT Libraries Government Documents Department

Microbial reduction of SO{sub 2} and NO{sub x} as a means of by- product recovery/disposal from regenerable processes for the desulfurization of flue gas. Technical progress report, June 11, 1992--September 11, 1992

Description: Based on the work described simultaneous SO{sub 2}/No{sub x} removal from flue gas based on direct contact of the gas with SRB and T. denitrificans co-cultures or cultures-in-series has been eliminated as a viable process concept at this time. The technical reasons are as follows: (1) NO inhibition of SO{sub 2} reduction by D. desulfuricans - Although the NO concentrations used in the experiments described above are somewhat higher than that found in a typical flue gas, it is quite possible that at lower NO concentrations (or partial pressures) the inhibiting effects will simply take longer to become apparent. (2) Nitrate suppression of NO removal - As noted previously, the cultivation of T. denitrificans in a microbial flue gas treatment system (either one or two stages) would require sulfide-limiting conditions. Therefore, the electron acceptor must be in excess, requiring nitrate in the T. denitrificans process culture. As shown in experiments described above, nitrate significantly suppresses the removal of NO from a feed gas making simultaneous SO{sub 2}/NO{sub x} removal impractical by microbial means. (3) O{sub 2} inhibition of SO{sub 2} and NO reduction - It has been demonstrated that D. desulfuricans working cultures are tolerant of up to 1.7% O{sub 2} in the feed gas. However, further increases in the O{sub 2} partial pressure in the feed gas resulted in O{sub 2} inhibition of SO{sub 2} reduction. These inhibiting levels of O{sub 2} are comparable to those concentrations found in flue gases (3). Therefore, in any process in which raw flue gas contacts a D. desulfuricans culture marginal stability at best can be expected.
Date: December 31, 1992
Creator: Sublette, K. L.
Partner: UNT Libraries Government Documents Department

Microbial field pilot study

Description: The objective of this project is to perform a microbially enhanced oil recovery field pilot test in the Southeast Vassar Vertz Sand Unit (SEVVSU) in Payne County, Oklahoma. Indigenous, anaerobic, nitrate-reducing bacteria will be stimulated to selectively plug flow paths which have been preferentially swept by a prior waterflood. This will force future flood water to invade bypassed regions or the reservoir and increase sweep efficiency. Injection of nutrient stimulates the growth and metabolism of reservoir bacteria, which produces beneficial products to enhance oil recovery. Sometimes, chemical treatments are used to clean or condition injection water. Such a chemical treatment has been initiated by Sullivan and Company at the Southeast Vassar Vertz Sand Unit. The unit injection water was treated with a mixture of water, methanol, isopropyl alcohol, and three proprietary chemicals. To determine if the chemicals would have an impact on the pilot, it was important to determine the effects of the chemical additives on the growth and metabolism of the bacteria from wells in this field. Two types of media were used: a mineral salts medium with molasses and nitrate, and this medium with 25 ppm of the treatment chemicals added. Samples were collected anaerobically from each of two wells, 1A-9 and 7-2. A sample from each well was inoculated and cultured in the broth tubes of molasses-nitrate medium with and without the chemicals. Culturing temperature was 35{degrees}C. Absorbance, pressure and cell number were checked to determine if the chemicals affected the growth and metabolism of bacteria in the brine samples. 12 figs.
Date: January 1, 1991
Creator: Knapp, R.M.; McInerney, M.J. & Menzie, D.E.
Partner: UNT Libraries Government Documents Department