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The Generation of Recombinant Zea mays Spastin and Katanin Proteins for In Vitro Analysis

Description: Plant microtubules play essential roles in cell processes such as cell division, cell elongation, and organelle organization. Microtubules are arranged in highly dynamic and ordered arrays, but unlike animal cells, plant cells lack centrosomes. Therefore, microtubule nucleation and organization are governed by microtubule-associated proteins, including a microtubule-severing protein, katanin. Mutant analysis and in vitro characterization has shown that the highly conserved katanin is needed fo… more
Date: December 2017
Creator: Alodailah, Sattam Sonitan
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Colchicine Reversibly Inhibits Electrical Activity in Arthropod Mechanoreceptors

Description: Dendrites of cockroach tibial spine mechanoreceptors contain hundreds of free microtubules, which may have some relation to the generation of electrical activity. Deflection of a spine produces a train of action potentials. Continuous perfusion over a period of 4 hours results in no response decrement. Perfusion with 10mM colchicine reversibly inhibits the response within 5-7 minutes. Irreversible inhibition is produced by perfusion with 1mM vinblastine sulfate in perfusion solution containing … more
Date: August 1977
Creator: Reagan, Paul D.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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A Genetic Approach to Identify Proteins that Interact with Eukaryotic Microtubule Severing Proteins via a Yeast Two Hybrid System

Description: Microtubules (MT) are regulated by multiple categories of proteins, including proteins responsible for severing MTs that are therefore called MT-severing proteins. Studies of katanin, spastin, and fidgetin in animal systems have clarified that these proteins are MT-severing. However, studies in plants have been limited to katanin p60, and little is known about spastin or fidgetin and their function in plants. I looked at plant genomes to identify MT-severing protein homologues to clarify which … more
Date: May 2020
Creator: Alhassan, Hassan H
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Growth and development of maize that contains mutant tubulin genes

Description: Mutant maize plants containing a Mu transposon disrupting one of the five beta tubulin genes of interest were followed for several generations and hybridized with each other to produce plants containing disruptions in both copies of a single gene or disruption of more than one tubulin gene. Seedlings of some of these plants were grown under chilling conditions for a few weeks. After DOE funding ended, plants have been assessed to see whether mutant are more or less tolerant to chilling. Other m… more
Date: July 23, 2004
Creator: Wick, Susan M.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Molecular architecture of axonemal microtubule doublets revealedby cryo-electron tomography

Description: The axoneme, which forms the core of eukaryotic flagella and cilia, is one of the largest macromolecular machines with a structure that is largely conserved from protists to mammals. Microtubule doublets are structural components of axonemes containing a number of proteins besides tubulin, and are usually found in arrays of nine doublets arranged around two singlet microtubules. Coordinated sliding of adjacent doublets, which involves a host of other proteins in the axoneme, produces periodic b… more
Date: May 22, 2006
Creator: Sui, Haixin & Downing, Kenneth H.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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Structural insights into microtubule doublet interactions inaxonemes

Description: Coordinated sliding of microtubule doublets, driven by dynein motors, produces periodic beating of the axoneme. Recent structural studies of the axoneme have used cryo-electron tomography to reveal new details of the interactions among some of the multitude of proteins that form the axoneme and regulate its movement. Connections among the several sets of dyneins, in particular, suggest ways in which their actions may be coordinated. Study of the molecular architecture of isolated doublets has p… more
Date: June 6, 2007
Creator: Downing, Kenneth H. & Sui, Haixin
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Time-Dependent Measure of a Nano-Scale Force-Pulse Driven by the Axonemal Dynein Motors in Individual Live Sperm Cells

Description: Nano-scale mechanical forces generated by motor proteins are crucial to normal cellular and organismal functioning. The ability to measure and exploit such forces would be important to developing motile biomimetic nanodevices powered by biological motors for Nanomedicine. Axonemal dynein motors positioned inside the sperm flagellum drive microtubule sliding giving rise to rhythmic beating of the flagellum. This force-generating action makes it possible for the sperm cell to move through viscous… more
Date: April 23, 2009
Creator: Allen, M J; Rudd, R E; McElfresh, M W & Balhorn, R
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

The Beginning of Kinesin's Force-Generating Cycle Visualized at 9Angstrom Resolution

Description: We have used cryo-electron microscopy of kinesin-decorated microtubules to resolve the structure of the motor protein kinesin's crucial nucleotide response elements, switch I and the switch II helix, in kinesin's poorly understood nucleotide-free state. Both of the switch elements undergo conformational change relative to the microtubule-free state. The changes in switch I suggest a role for it in ''ejecting'' adenosine diphosphate when kinesin initially binds to the microtubule. The switch II … more
Date: June 20, 2007
Creator: Sindelar, Charles V. & Downing, Kenneth H.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Effect of Colchicine on Neuronal Excitabilty

Description: The abundance of microtubules in receptive dendrites suggests they may function in sensory transduction. Responses of frog muscle spindle receptors and joint receptors is inhibited within 25 minutes by 50 mM colchicine, a microtubuledisrupting agent. The inhibition is reversible upon removal of colchicine, and the time course of recovery is comparable to that of inhibition. Frog olfactory responses are briefly inhibited by washing the olfactory mucosa with perfusion fluid. Colchicine accentuate… more
Date: August 1978
Creator: Okafo, Ngozi
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

The Cotton Kinesin-Like Calmodulin-Binding Protein Associates with Cortical Microtubles in cotton Fibers

Description: Microtubules in interphase plant cells form a cortical array, which is critical for plant cell morphogenesis. Genetic studies imply that the minus end-directed microtubule motor kinesin-like calmodulin-binding protein (KCBP) plays a role in trichome morphogenesis in Arabidopsis. However, it was not clear whether this motor interacted with interphase microtubules. In cotton (Gossypium hirsutum) fibers, cortical microtubules undergo dramatic reorganization during fiber development. In this study,… more
Date: May 1, 2003
Creator: L., Preuss M.; Delmar, D.P. & Liu, Bo
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

YY1 modulates taxane response in epithelial ovarian cancer

Description: The results of this study show that a high YY1 gene signature (characterized by coordinate elevated expression of transcription factor YY1 and putative YY1 target genes) within serous epithelial ovarian cancers is associated with enhanced response to taxane-based chemotherapy and improved survival. If confirmed in a prospective study, these results have important implications for the potential future use of individualized therapy in treating patients with ovarian cancer. Identification of the Y… more
Date: October 10, 2008
Creator: Matsumura, Noriomi; Huang, Zhiqing; Baba, Tsukasa; Lee, Paula S.; Barnett, Jason C.; Mori, Seiichi et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Role of Ca{sup ++}/calmodulin in the regulation of microtubules in higher plants. Progress report, FY91

Description: This work is aimed at defining the role of calcium/calmodulin in regulating cortical microtubules (MTS) in higher plants. Recent thrust has been to define the effects of calcium upon microtubules in vivo. Using lysed protoplasts, we noted Mts are destabilized by calcium/calmodulin. This effect could be the result of gross depolymerization induced by Ca{sup ++}/calmodulin, or by an increase in the dynamic flux rate. Intact protoplasts exposed to high (10 mM) levels of calcium (which would be exp… more
Date: December 31, 1991
Creator: Cyr, R.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Mechanisms and chemical induction of aneuploidy in rodent germ cells

Description: The objective of this review is to suggest that the advances being made in our understanding of the molecular events surrounding chromosome segregation in non-mammalian and somatic cell models be considered when designing experiments for studying aneuploidy in mammalian germ cells. Accurate chromosome segregation requires the temporal control and unique interactions among a vast array of proteins and cellular organelles. Abnormal function and temporal disarray among these, and others to be inid… more
Date: October 15, 2004
Creator: Mailhes, J B & Marchetti, F
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Role of Ca{sup ++}/calmodulin in the regulation of microtubules in higher plants. Progress report, FY 1992

Description: The cytoskeleton including its microtubule (Mt) component participates in processes that directly affect growth and development in higher plants. Normal cytoskeletal function requires the precise and orderly arrangement of Mts into several cell cycle and developmentally specific arrays. The cortical array somehow directs the deposition of cellulose. Little molecular information is available regarding the formation of these arrays or the cellular signals to which they respond. Experimental data … more
Date: December 31, 1992
Creator: Cyr, R.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Active Transport of Nanomaterials Using Motor Proteins Final report for DOE-BES grant DE-FG03-03ER46024

Description: During the two year period of funding we have focused on the following topics: Guiding of microtubule movement on kinesin-coated, structured surfaces, directed assembly of oriented microtubule networks, and the interaction between synthetic materials and biological components in hybrid devices based on microtubules and kinesin motors. Additional efforts have been made and are still on- going in controlling the motor activity, and loading and unloading of cargo. In all aspects, the collaboration… more
Date: March 16, 2005
Creator: Hess, Henry
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Self-assembling holographic biosensors and biocomputers.

Description: We present concepts for self-assembly of diffractive optics with potential uses in biosensors and biocomputers. The simplest such optics, diffraction gratings, can potentially be made from chemically-stabilized microtubules migrating on nanopatterned tracks of the motor protein kinesin. We discuss the fabrication challenges involved in patterning sub-micron-scale structures with proteins that must be maintained in aqueous buffers to preserve their activity. A novel strategy is presented that em… more
Date: May 1, 2006
Creator: Light, Yooli Kim; Bachand, George David; Schoeniger, Joseph S. & Trent, Amanda M.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Active assembly for large-scale manufacturing of integrated nanostructures.

Description: Microtubules and motor proteins are protein-based biological agents that work cooperatively to facilitate the organization and transport of nanomaterials within living organisms. This report describes the application of these biological agents as tools in a novel, interdisciplinary scheme for assembling integrated nanostructures. Specifically, selective chemistries were used to direct the favorable adsorption of active motor proteins onto lithographically-defined gold electrodes. Taking advanta… more
Date: January 1, 2007
Creator: Spoerke, Erik David; Bunker, Bruce Conrad; Orendorff, Christopher J.; Bachand, George David; Hendricks, Judy K. & Matzke, Carolyn M.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Calmodulin immunolocalization to cortical microtubules is calcium independent

Description: Calcium affects the stability of cortical microtubules (MTs) in lysed protoplasts. This calmodulin (CaM)-mediated interaction may provide a mechanism that serves to integrate cellular behavior with MT function. To test the hypothesis that CaM associates with these MTs, monoclonal antibodies were produced against CaM, and one (designated mAb1D10), was selected for its suitability as an immunocytochemical reagent. It is shown that CaM associates with the cortical Mats of cultured carrot (Daucus c… more
Date: December 31, 1992
Creator: Fisher, D. D. & Cyr, R. J.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Cellulose and the Control of Growth Anisotropy

Description: The authors research aims to understand morphogenesis, focusing on growth anisotropy, a process that is crucial to make organs with specific and heritable shapes. For the award, the specific aims were to test hypotheses concerning how growth anisotropy is controlled by cell wall structure, particularly by the synthesis and alignment of cellulose microfibrils, the predominant mechanical element in the cell wall. This research has involved characterizing the basic physiology of anisotropic expans… more
Date: April 1, 2004
Creator: Baskin, Tobias I.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Engineering intracellular active transport systems as in vivo biomolecular tools.

Description: Active transport systems provide essential functions in terms of cell physiology and metastasis. These systems, however, are also co-opted by invading viruses, enabling directed transport of the virus to and from the cell's nucleus (i.e., the site of virus replication). Based on this concept, fundamentally new approaches for interrogating and manipulating the inner workings of living cells may be achievable by co-opting Nature's active transport systems as an in vivo biomolecular tool. The over… more
Date: November 1, 2006
Creator: Bachand, George David & Carroll-Portillo, Amanda
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Active Transport of Nanomaterials Using Motor Proteins -Final Report

Description: During the six months of funding we have focused first on the completion of the research begun at the University of Washington in the previous funding cycle. Specifically, we developed a method to polymerize oriented networks of microtubules on lithographically patterned surfaces (M.S. thesis Robert Doot). The properties of active transport have been studied detail, yielding insights into the dispersion mechanisms (Nitta et al.). The assembly of multifunctional structures with a microtubule cor… more
Date: October 21, 2005
Creator: Hess, Henry
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Assembly and actuation of nanomaterials using active biomolecules.

Description: The formation and functions of living materials and organisms are fundamentally different from those of synthetic materials and devices. Synthetic materials tend to have static structures, and are not capable of adapting to the functional needs of changing environments. In contrast, living systems utilize energy to create, heal, reconfigure, and dismantle materials in a dynamic, non-equilibrium fashion. The overall goal of the project was to organize and reconfigure functional assemblies of nan… more
Date: November 1, 2005
Creator: Spoerke, Erik David; Thayer, Gayle Echo; de Boer, Maarten Pieter; Bunker, Bruce Conrad; Liu, Jun; Corwin, Alex David et al.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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