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Biocompatible self-assembly of nano-materials for Bio-MEMS and insect reconnaissance.

Description: This report summarizes the development of new biocompatible self-assembly procedures enabling the immobilization of genetically engineered cells in a compact, self-sustaining, remotely addressable sensor platform. We used evaporation induced self-assembly (EISA) to immobilize cells within periodic silica nanostructures, characterized by unimodal pore sizes and pore connectivity, that can be patterned using ink-jet printing or photo patterning. We constructed cell lines for the expression of flu… more
Date: December 1, 2003
Creator: Brozik, Susan Marie; Cesarano, Joseph, III; Brinker, C. Jeffrey; Dunphy, Darren Robert; Sinclair, Michael B.; Manginell, Monica et al.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Single-cell bioluminescence and GFP in biofilm research

Description: Using flow cells and a combination of microscopy techniques, we can unequivocally identify single bacterial cells that express bioluminescent and fluorescent bioreporters. We have shown that, for attached cells, bioluminescence output within a bacterial strain can vary greatly from cell to cell.
Date: December 31, 1996
Creator: Palmer, R.J. Jr, Sayler, G., White, D.C. & Phiefer, C.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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Production of ethanol from starch by co-immobilized Zymomonas mobilis -- Glucoamylase in a fluidized-bed reactor

Description: The production of ethanol from starch was studied in a fluidized-bed reactor (FBR) using co-immobilized Zymomonas mobilis and glucoamylase. The FBR was a glass column of 2.54 cm in diameter and 120 cm in length. The Z. mobilis and glucoamylase were co-immobilized within small uniform beads (1.2 to 2.5 mm diameter) of {kappa}-carrageenan. The substrate for ethanol production was a soluble starch. Light steep water was used as the complex nutrient source. The experiments were performed at 35 C an… more
Date: August 1, 1997
Creator: Sun, M. Y.; Davison, B. H.; Bienkowski, P. R.; Nghiem, N. P. & Webb, O.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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Biocarrier Composition for and Method of Degrading Pollutants

Description: The present invention relates to biocarrier compositions that attract and bond pollutant-degrading antigens that will degrade the pollutants. Biocarriers are known generally as a variety of inert or semi-inert compounds or structures having the ability to sequester (attract), hold and biomagnify (enhance) specific microorganisms within their structure. Glass or polystyrene beads are the most well known biocarriers. The biocarrier, which is preferably in the form of glass microspheres, is coated… more
Date: January 1, 1994
Creator: Fliermans, Carl B.
Item Type: Refine your search to only Patent
Partner: UNT Libraries Government Documents Department
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Bioremediation of aqueous pollutants using biomass embedded in hydrophilic foam. Final report

Description: The major objective of this project was to examine the potential of a novel hydrophilic polyurethane foam as an immobilization medium for algal, bacteria, and other types of biomass, and to test the resulting foam/biomass aggregates for their use in cleaning up waters contaminated with heavy metals, radionuclides and toxic organic compounds. Initial investigations focused on the bioremoval of heavy metals from wastewaters at SRS using immobilized algal biomass. This effort met with limited succ… more
Date: December 31, 1996
Creator: Wilde, E. W.; Radway, J. C.; Santo Domingo, J.; Zingmark, R. G. & Whitaker, M. J.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Degradation of mix hydrocarbons by immobilized cells of mix culture using a trickle fluidized bed reactor. Annual progress report, June 1992--May 1993

Description: The microorganisms, capable of degrading mix hydrocarbons were isolated from the soil samples collected from the hydrocarbon contaminated sites. The mix cultures were immobilized in calcium alginate solution in the form of beads. A trickle fluidized bed air-uplift-type reactor designed to study the degradation of mix hydrocarbons was filled with 0.85% normal saline containing the immobilized cells of mix culture. The immobilized beads were aerated with CO{sub 2}-free air at 200 ml/min. The degr… more
Date: June 1, 1993
Creator: Chapatwala, K. D.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Microbial reduction of SO{sub 2} and NO{sub x} as a means of by-product recovery/disposal from regenerable processes for the desulfurization of flue gas. Technical progress report, December 11, 1992--March 11, 1993

Description: This report describes the potential of sulfate reducing bacteria to fix sulfur derived from flue gas desulfurization. The first section reviews the problem, the second section reviews progress of this study to use desulfovibrio desulfuricans for this purpose. The final section related progress during the current reporting period. This latter section describes studies to immobilize the bacteria in co-culture with floc-forming anaerobes, use of sewage sludges in the culture media, and sulfate pro… more
Date: December 31, 1993
Creator: Sublette, K. L.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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The production of chemicals from food processing wastes using a novel fermenter separator. Annual progress report, January 1993--March 1994

Description: The basic objective of this project is to convert waste streams from the food processing industry to usable fuels and chemicals using novel bioreactors. These bioreactors should allow economical utilization of waste (whey, waste sugars, waste starch, bottling wastes, candy wastes, molasses, and cellulosic wastes) by the production of ethanol, acetone/butanol, organic acids (acetic, lactic, and gluconic), yeast diacetyl flavor, and antifungal compounds. Continuous processes incorporating various… more
Date: March 15, 1994
Creator: Dale, M. C.; Venkatesh, K. V.; Choi, H.; Salicetti-Piazza, L.; Borgos-Rubio, N.; Okos, M. R. et al.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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PH-NEUTRAL CONCRETE FOR ATTACHED MICROALGAE AND ENHANCED CARBON DIOXIDE FIXATION - PHASE I

Description: The novelty/innovation of the proposed work is as follows. Supercritical carbon dioxide (SC-CO {sub 2})-based extrusion and molding technology can be used to produce significantly improved (in terms of strength/unit weight, durability, hardness and chemical resistance) cement-based products. SC-CO{sub 2} can rapidly convert the calcium hydroxide in cured cement to calcium carbonate, which increases the density and unconfined compressive strength in the treated region. In cured concrete, this tr… more
Date: May 31, 1999
Creator: Dooley, Kerry M.; Knopf, F. Carl & Gambrell, Robert P.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Reversibly immobilized biological materials in monolayer films on electrodes

Description: A method is provided for reversibly binding charged biological particles in a fluid medium to an electrode surface. The method comprises treating (e.g., derivatizing) the electrode surface with an electrochemically active material; connecting the electrode to an electrical potential; and exposing the fluid medium to the electrode surface in a manner such that the charged particles become adsorbed on the electrode surface.
Date: April 8, 1991
Creator: Weaver, P. F. & Frank, A. J.
Item Type: Refine your search to only Patent
Partner: UNT Libraries Government Documents Department
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High Pressure Synthesis Gas Conversion. Final Report

Description: The purpose of this research project is to build and test a high pressure fermentation system for the production of ethanol from synthesis gas. The fermenters, pumps, controls, and analytical system were procured or fabricated and assembled in our laboratory. This system was then used to determine the effects of high pressure on growth and ethanol production by Clostridium ljungdahlii. The limits of cell concentration and mass transport relationships were found in CSTR and immobilized cell reac… more
Date: May 1, 1993
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Microbial reduction of SO{sub 2} and NO{sub x} as a means of by- product recovery/disposal from regenerable processes for the desulfurization of flue gas. Technical progress report, September 11, 1991--December 11, 1992

Description: A review of the author`s work on microbial reduction of flue gases is provided. The work begins with a discussion of efforts preceding the current project, then reviews the progress made in earlier periods of the project and concludes with a report of progress made in the current reporting period, September 11, 1991 to December 11, 1992.
Date: December 31, 1992
Creator: Sublette, K. L.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Bioconversion of coal derived synthesis gas to liquid fuels. Quarterly technical progress report, 1 April--30 June 1994

Description: The overall objective of the project is to develop an integrated two-stage fermentation process for conversion of coal-derived synthesis gas to a mixture of alcohols. This is achieved in two steps. In the first step, Butyribacterium methylotrophicum converts carbon monoxide (CO) to butyric and acetic acids. Subsequent fermentation of the acids by Clostridium acetobutylicum leads to the production of butanol and ethanol. The tasks for this quarter were: development/isolation of superior strains … more
Date: July 18, 1994
Creator: Jain, M. K.; Worden, R. M. & Grethlein, A.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Review and evaluation of immobilized algae systems for the production of fuels from microalgae. Final subcontract report

Description: The purpose of this paper is to review and evaluate the use of immobilized algae systems. It was the finding that commercial immobilized algae systems are not in operation at this time but, with research, could certainly become so. The use of immobilized algae will depend on, as in all commercial systems, the economic value of the product. This paper reviews the technical feasibility of immobilization as it applies to algae. Finally, the economics of possible immobilized algal systems that woul… more
Date: November 1, 1985
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Removal of CO{sub 2} from flue gases by algae. [Quarterly] technical report, March 1, 1993--May 31, 1993

Description: The objective of this research program is to determine the feasibility of the alga Botryococcus braunii as a biocatalyst for the photosynthetic conversion of flue gas CO{sub 2} to hydrocarbons. The research program involves the determination of the biocatalytic characteristics of free and immobilized cultures of Botryococcus braunii in bench-scale studies, and the feasibility study and economic analysis of the Botryococcus braunii culture systems for the conversion of flue gas CO{sub 2} to hydr… more
Date: September 1, 1993
Creator: Akin, C. & Pradhan, S.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Removal of CO{sub 2} from flue gases by algae. Technical report, December 1, 1992--February 28, 1993

Description: The studies reported here confirmed our preliminary observations that Botryococcus braunii can tolerate and grow well in flue gas CO{sub 2} concentrations of 10 to 15%, and produce oil. The highest extracted oil was observed in 10% CO{sub 2} enriched air. Initial pH of the medium at or near 10 pH is favorable to cell growth probably by stimulating the CO{sub 2} solubilization in the medium. This is also indicated in Botryococcus braunii growth and oil formation in NaHCO{sub 3} added medium. The… more
Date: May 1, 1993
Creator: Akin, C.; Maka, A.; Pradhan, S. & Banerjee, D.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Degradation of mix hydrocarbons by immobilized cells of mix culture using a trickle fluidized bed reactor. Final report: June 1992--June 1994

Description: The microorganisms capable of degrading mix hydrocarbons were isolated from the soil samples collected from the hydrocarbon contaminated sites. The mix cultures were identified as Pseudomonas acidovorans, Flavobacterium indoltheticum and Phyllobacterium rubiaceum. The bacterial cells of mix cultures were immobilized in calcium-alginate solution in the form of beads. A trickle fluidized bed air-uplift-type reactor designed to study the degradation of mix hydrocarbons was filled with 0.85% normal… more
Date: December 1, 1994
Creator: Chapatwala, K. D.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Microbial reduction of SO{sub 2} and NO{sub x} as a means of by-product recovery/disposal from regenerable processes for the desulfurization of flue gas. Technical progress report, September 11, 1992--December 11, 1992

Description: With the continual increase in the utilization of high sulfur and high nitrogen containing fossil fuels, the release of airborne pollutants into the environment has become a critical problem. The fuel sulfur is converted to SO{sub 2} during combustion. Fuel nitrogen and a fraction of the nitrogen from the combustion air are converted to nitric oxide and nitrogen dioxide, NO{sub x}. For the past five years Combustion Engineering (now Asea Brown Boveri or ABB) and, since 1986, the University of T… more
Date: December 31, 1992
Creator: Sublette, K. L.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Degradation of mix hydrocarbons by immobilized cells of mix culture using a trickle fluidized bed reactor

Description: The microorganisms, capable of degrading mix hydrocarbons were isolated from the soil samples collected from the hydrocarbon contaminated sites. The mix cultures were immobilized in calcium alginate solution in the form of beads. A trickle fluidized bed air-uplift-type reactor designed to study the degradation of mix hydrocarbons was filled with 0.85% normal saline containing the immobilized cells of mix culture. The immobilized beads were aerated with CO[sub 2]-free air at 200 ml/min. The degr… more
Date: January 1, 1993
Creator: Chapatwala, K.D.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Biological Upgrading of Coal-Derived Synthesis Gas: Final Report

Description: The technical feasibility of the biological conversion of coal synthesis gas to methane has been demonstrated in the University of Arkansas laboratories. Cultures of microorganisms have been developed which achieve total conversion in the water gas shift and methanation reactions in either mixed or pure cultures. These cultures carry out these conversions at ordinary temperatures and pressures, without sulfur toxicity. Several microorganisms have been identified as having commercial potential f… more
Date: October 1, 1986
Creator: Barik, S.; Johnson, E. R.; Ko, C. W.; Clausen, E. C. & Gaddy, J. L.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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[Physicochemical and microbiological factors influencing the bioavailability of organic contaminants in subsoils]. Progress report, [July 1, 1992--June 30, 1993]

Description: We report progress in elucidating the microbiological variables important in determining the relative success of bacteria in utilizing soil-sorbed contaminants. Two bacterial species, Pseudomonas putida (ATCC 17484) and an Alcaligenes sp. isolated from petroleum contaminated soil are known to differ markedly in their ability to utilize soil-sorbed napthalene based on a kinetic comparison of their capability of naphthalene mineralization in soil-containing and soil-free systems. The kinetic anal… more
Date: December 31, 1992
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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The ultimate ethanol: Technoeconomic evaluation of ethanol manufacture, comparing yeast vs Zymomonas bacterium fermentations. [Zymomonas mobilis:a5; Saccharomyces cerevisiae:a6]

Description: If ethanol could be produced at a low enough price to serve as the precursor to ethylene and butadiene, it and its derivatives could account for 159 billion lb, or 50% of the US production of 316 billion lb of synthetic organic chemicals, presently valued at $113 billion. This use would consume 3.4 billion bu of corn, or {approximately}40% of the corn crop. This study evaluates advance process engineering and genetic engineering techniques that could generate savings and reduce production costs… more
Date: August 1, 1991
Creator: Busche, R. M. (Bio En-Gene-Er Associates, Inc., Wilmington, DE (United States)); Scott, C. D.; Davison, B. H. (Oak Ridge National Lab., TN (United States)) & Lynd, L. R. (Dartmouth Coll., Hanover, NH (United States))
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Microbial reduction of SO[sub 2] and NO[sub x] as a means of by- product recovery/disposal from regenerable processes for the desulfurization of flue gas

Description: A review of the author's work on microbial reduction of flue gases is provided. The work begins with a discussion of efforts preceding the current project, then reviews the progress made in earlier periods of the project and concludes with a report of progress made in the current reporting period, September 11, 1991 to December 11, 1992.
Date: January 1, 1992
Creator: Sublette, K.L.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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