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Construction of a Cloning Vector Based upon a Rhizobium Plasmid Origin of Replication and its Application to Genetic Engineering of Rhizobium Strains

Description: Rhizobia are Gram-negative, rod-shaped, soil bacteria with the ability to fix atmospheric nitrogen into ammonia as symbiont bacteroids within nodules of leguminous plant roots. Here, resident Rhizobium plasmids were studied as possible sources of components for the construction of a cloning vector for Rhizobium species.
Date: May 1992
Creator: Jeong, Pyengsoo
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

A Novel Mechanism for Site-Directed Mutagenesis of Large Catabolic Plasmids Using Natural Transformation

Description: Natural transformation is the process by which cells take up DNA from the surrounding medium under physiological conditions, altering the genotype in a heritable fashion. This occurs without chemical or physical treatment of the cells. Certain Acinetobacter strains exhibit a strong tendency to incorporate homologous DNA into their chromosomes by natural transformation. Transformation in Acinetobacter exhibits several unique properties that indicate this system's superiority as a model for tra… more
Date: August 2001
Creator: Williamson, Phillip C.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

New developments in biotechnology: field-testing engineered organisms: genetic and ecological issues: contractor documents, volume 2

Description: This report includes these topics: Ecological issues relevant to environmental applications of genetically altered organisms / Elliott A. Norse -- An ecosystems approach to potential perturbations of energy flow and nutrient cycles associated with environmental applications of genetically altered organisms / David C. Coleman and Robert E. Hodson -- Ecological impact of genetically engineered organisms on ecosystems / James R. Gosz, C.N. Dahm, and Patrick W. Flanagan -- The genetic basis of chan… more
Date: December 2, 1986
Creator: United States. Congress. Office of Technology Assessment
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Genetically Engineered Salmon

Description: This report discusses the genetically modified salmon. The term “genetic modification” refers to changes in an organism’s genetic makeup that do not occur in nature. Also, if approved by the Food and Drug Administration (FDA), Atlantic salmon would be the first genetically engineered (GE) animal to be marketed in the United States for human consumption.
Date: April 30, 2014
Creator: Upton, Harold F. & Cowan, Tadlock
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Gene Patents: A Brief Overview of Intellectual Property Issues

Description: This report is a brief discussion of the ethical, legal, and economic issues of gene patenting. The courts have upheld gene patents that meet the criteria of patentability defined by the Patent Act. However, the practice of awarding patents on genes has come under intense scrutiny by some scientists, legal scholars, politicians, and other experts.
Date: October 3, 2006
Creator: Schacht, Wendy H.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Genetic Exceptionalism: Genetic Information and Public Policy

Description: This report provides an overview of the nature of genetic information and its implications for individuals, family, and society. Individuals utilize genetic information to guide health care and other decisions, when possible, and may experience anxiety as a result of genetic test results. Genetic test results for an individual may often be informative for other close family members and thus influence their care decisions. Society must grapple with the effect genetic information may have on our … more
Date: February 14, 2008
Creator: Sarata, Amanda K.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Labeling of Genetically Modified Foods

Description: Congressional interest in the labeling of genetically modified foods (GM foods) has been rising. So far, two bills have been introduced to mandate that all foods from genetically modified crops (GM crops) be labeled as such. The Food and Drug Administration (FDA) issued its current GM food labeling policy in May 1992. At that time, the agency determined that it would regulate GM foods no differently than foods created by conventional means because FDA considered them substantially equivalent t… more
Date: March 20, 2000
Creator: Vogt, Donna U. & Jackson, Brian A.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Agricultural Biotechnology: Background, Regulation, and Policy Issues

Description: This report discusses on going issues regarding biotechnology, which refers primarily to the use of recombinant DNA techniques to genetically modify or bioengineer plants and animals. Ongoing policy issues include the impacts of genetially engineered (GE) crops on the environment (e.g., pest and weed resistance), whether GE foods should be labeled, their potential contamination of conventionally raised and organic plants, and issues of liability.
Date: April 3, 2013
Creator: Cowan, Tadlock
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Agricultural Biotechnology: Background, Regulation, and Policy Issues

Description: This report discusses on going issues regarding biotechnology, which refers primarily to the use of recombinant DNA techniques to genetically modify or bioengineer plants and animals. Ongoing policy issues include the impacts of genetially engineered (GE) crops on the environment (e.g., pest and weed resistance), whether GE foods should be labeled, their potential contamination of conventionally raised and organic plants, and issues of liability.
Date: July 20, 2015
Creator: Cowan, Tadlock
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

DNA Sliding Clamps: Just the Right Twist to Load onto DNA

Description: Two recent papers illuminate a long sought step in DNA sliding clamp loading. One paper reveals the structure of the PCNA clamp wrapped around DNA--still open from being loaded--while a second paper discovers that the clamp may assist this process by forming a right-handed helix upon opening.
Date: October 24, 2005
Creator: Barsky, D & Venclovas, C
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Advanced Gene Editing: CRISPR-Cas9

Description: This report describes a new gene editing technology, known as CRISPR-Cas9, with the potential to revolutionize genetic engineering and the biotechnology industry. The report then provides information on the potential economic benefits of the technology and identifies some issues for congressional consideration, including the regulation of current and future products, national security concerns, and ethical and societal issues surrounding the use of the technology.
Date: April 28, 2017
Creator: Gallo, Marcy E.; Sargent, John F., Jr.; Sarata, Amanda K. & Cowan, Tadlock
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department

Genetic Modification of Fatty Acid Profiles in Cotton

Description: The industrial uses of cottonseed oil are limited by its fatty acid composition. Genetic modification of cotton lipid profiles using seed-specific promoters could allow cotton growers to produce valuable new oils in the seed without adverse effects on fiber quality and yield, therefore making this crop more commercially profitable. Transgenic cotton callus harboring a diverged fatty acid desaturase gene (FADX) from Momordica charantia was characterized for production of alpha-eleostearic aci… more
Access: Restricted to the UNT Community Members at a UNT Libraries Location.
Date: August 2005
Creator: Rommel, Amy A.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

CBM.DIAGB.03.10.LLNL.007 Final Report

Description: The purpose of this project was to construct a system for characterizing the threat potential of genomic sequences, specifically assembled draft genomes. New genomes are characterized by initially comparing them against already-sequenced genomes. If the new genome is determined to be from a high-threat species, detailed (forensic-level) characterization is done based on gene and SNP (Single Nucleotide Polymorphism) data comparisons with all other previously sequenced members of that high-threat… more
Date: March 30, 2011
Creator: Slezak, T & Torres, M
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Final Report: The DNA Files: Unraveling the mysteries of genetics, January 1, 1998-March 31, 1999

Description: The DNA Files is an award-winning radio documentary series on genetics created by SoundVision Productions. The DNA Files was hosted by John Hockenberry and was presented in documentary and discussion format. The programs covered a range of topics from prenatal and predictive gene testing, gene therapy, and commercialization of genetic information to new evolutionary genetic evidence, transgenic vegetables and use of DNA in forensics.
Date: May 1, 1999
Creator: Scott, Bari
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Biodiesel from aquatic species. Project report: FY 1993

Description: Researchers in the Biodiesel/Aquatic Species Project focus on the use of microalgae as a feedstock for producing renewable, high-energy liquid fuels. The program`s basic premise is that microalgae, which have been called the most productive biochemical factories in the world, can produce up to 30 times more oil per unit of growth area than land plants. It is estimated that 150 to 400 barrels of oil per acre per year (0.06 to 0.16 million liters/hectar) could be produced with microalgal oil tech… more
Date: January 1, 1994
Creator: Brown, L. M.; Sprague, S.; Jarvis, E. E.; Dunahay, T. G.; Roessler, P. G. & Zeiler, K. G.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Development of combinatorial bacteria for metal and radionuclide bioremediation

Description: The grant concerned chromate [Cr(VI)] bioremediation and it was our aim from the outset to construct individual bacterial strains capable of improved bioremediation of multiple pollutants and to identify the enzymes suited to this end. Bacteria with superior capacity to remediate multiple pollutants can be an asset for the cleanup of DOE sites as they contain mixed waste. I describe below the progress made during the period of the current grant, providing appropriate context.
Date: June 15, 2006
Creator: Matin, A. C.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

A New Selectable Marker System for Genetic Studies of Bacteria: Final Report

Description: Genetic manipulations in bacteria currently rely on the introduction of antibiotic resistance genes into a bacterial strain; for those organisms that will be used for commercial or industrial applications, the genetic cassette encoding the antibiotic resistance is sometimes removed after selection. it is clear that if alternative technologies could obviate the need to introduce antibiotic resistance into bacteria, they would most certainly become a standard tool in molecular micriobiology for c… more
Date: March 18, 2011
Creator: Parsons, D; Tolmasky, M; Chain, P & Segelke, B W
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Genetically Engineered Fish and Seafood

Description: This is a report on Genetically Engineered Fish and seafood, with are supposed to have inherited traits to have improved organisms due to the genetical engineering.
Date: December 7, 2004
Creator: Borgatti, Rachel & Buck, Eugene H.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Impact of a Genetically Engineered Probiotic Therapy and IGF-1 Genomics in the PAHenu2 Mouse Model of PKU

Description: Absence of functional phenylalanine hydroxylase results in phenylketonuria (PKU). Viable treatments remain few, expensive and secondary conditions such as osteopenia occur in most PKU patients. Objective 1: Given the recently described roles of gut microbes to aid host digestion, an orally administered genetically engineered probiotic as the delivery vehicle for enzyme replacement therapy was created. The engineered probiotic, pHENOMMenal, produced phenylalanine ammonia lyase with significant… more
Date: December 2015
Creator: Durrer, Katherine Elaine
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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