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Prospects for the Precision Measurement of {alpha}{sub s}

Description: The prospects for the measurement of the strong coupling constant {alpha}{sub MS}(M{sub Z}) to a relative uncertainty of 1 % are discussed. Particular emphasis is placed on the implications relating to future High Energy Physics facilities.
Date: December 1996
Creator: Burrows, P. N.; Dixon, L. & El-Khadra, A. X.
Partner: UNT Libraries Government Documents Department

Relevance of in vivo models in melanoma skin cancer

Description: A discussion of possible wavelength dependence of induction of cutaneous malignant melanoma (CMM) is provided. Strengths and weaknesses of various experimental approaches to better understanding of the prevalence of CMM in different human populations including latitude effects are compared. Further the advantages and limitations of the use of the laboratory opossum (Monodelphis domestic), transgenic mice containing SV40 ongogene sequences under tyrosinase promoter control, and a backcross hybrid fish of the genus Xenophorus are contrasted.
Date: December 31, 1995
Creator: Setlow, R.B.
Partner: UNT Libraries Government Documents Department

Interim report on intrathoracic radiotherapy of human small-cell lung carcinoma in nude mice with Re-188-RC-160, a radiolabeled somatostatin analogue

Description: The purpose of this study was to evaluate the therapeutic efficacy of Re-188-RC-160 in experimental models of human small cell lung carcinomas which mimic the clinical presentation. In the experimental model, cells from the human small cell lung carcinoma cell line NCI-H69 cells were inoculated into the thoracic cavity of athymic mice and rats. Subsequently, the biodistribution of Re-188-RC-160 after injection into the pleural cavity, a radiolabeled somatostatin analogue, was monitored as was the effect on the subsequent growth of tumors. The results presented here, and which are a part of a larger series of studies, suggest that Re-188-RC-160 can be effectively used in this animal model to restrict the growth of small cell lung carcinoma in the thoracic cavity.
Date: July 1, 1995
Creator: Zamora, P.O.; Bender, H.; Biersack, H.J. & Knapp, F.F. Jr.
Partner: UNT Libraries Government Documents Department

Spatially fractionated microirradiation of normal CNS and gliosarcomas of the rat with synchrotron photons: Cell and tissue lesions; Raeumlich fraktionierte Mikrobestrahlung von normalem ZNS und Gliosarkomen der Ratte mit Synchrotron-Photonen: Zell- und Gewebelaesionen

Description: Rats were implanted intracerebrally with 9L gliosarcoma cells were used to experimentally determine the curative effectiveness of synchrotron radiation produced by the National Synchrotron Radiation Source. Radiation was delivered in beams with each ray 25 micrometers thick and arranged in an array of 4 mm corners. All experimental animals receiving the gliosarcoma cells and not treated died within four weeks. Treated animals surviving 113 days were sacrificed and their brains were examined histologically.
Date: December 31, 1995
Creator: Laissue, J.A.; Spanne, P.; Dilmanian, F.A.; Nawrocky, M.N.; Slatkin, D.N.; Joel, D.D. et al.
Partner: UNT Libraries Government Documents Department

BPA uptake in rat tissues after partial hepatectomy

Description: In boron neutron capture therapy (BNCT), boron given as boronophenylalanine (BPA) accumulates transiently not only in tumors but also in normal tissues. Average boron concentrations in transplanted 9L gliosarcoma tumors of 20 rats were 2.5 to 3.7 times concentrations found in blood. Although boron levels in a variety of tissues were also higher than blood the concentrations were less than the lowest found in the tumor. Further note than although BPA is a structural analogue of phenylalanine (Phe), the pathway of BPA uptake into regenerating liver may not be linked to Phe uptake mechanisms.
Date: December 31, 1996
Creator: Slatkin, D.N.; Nawrocky, M.M.; Coderre, J.A.; Fisher, C.D.; Joel, D.D.; Lombardo, D.T. et al.
Partner: UNT Libraries Government Documents Department

Radiogenic neoplasia in thyroid and mammary clonogens. Final progress report, 1 January 1987--31 December 1997

Description: The induction of cancer by ionizing radiation is a matter of great practical importance to the nuclear industry, to national defense, to radiological medicine and to the general public. It is increasingly apparent that carcinogenesis is a leading dose-limiting effect of radiation exposure. The thyroid and mammary glands are among the most sensitive human tissues to radiogenic initiation of cancer, and there is a profoundly higher risk of neoplastic initiation in these glands among individuals irradiated before or during puberty than among those exposed in later life. The authors developed unique quantitative experimental models to investigate and characterize the cells of origin of thyroid and mammary cancers and the effects of radiation on them (C185). To study these progenitor cells in vivo it is necessary to have a system by which their concentrations, total numbers and responses to radiation and other factors can be measured. It is a truism that not all cells in a tissue are equally sensitive to neoplastic initiation. They reasoned that the progenitor cells are most likely members of that subpopulation that is necessary to maintenance of normal tissue cell numbers and to repair and replacement after tissue damage. They further reasoned that such cells would likely be responsive to specific mitogenic stimulation by hormones. On the basis of these considerations, they developed quantitative rat thyroid and mammary epithelial cell transplantation systems.
Date: July 10, 1998
Creator: Clifton, K.H.
Partner: UNT Libraries Government Documents Department

Epidermal growth factor (EGF) as a potential targeting agent for delivery of boron to malignant gliomas

Description: The majority of high grade gliomas express an amplified epidermal growth factor receptor (EGFR) gene, and this often is associated with an increase in cell surface receptor expression. The rapid internalization and degradation of EGF-EGFR complexes, as well as their high affinity make EGF a potential targeting agent for delivery of {sup 10}B to tumor cells with an amplified number of EGFR. Human glioma cells can expresses as many as 10{sup 5} {minus}10{sup 6} EGF receptors per cell, and if these could be saturated with boronated EGF, then > 10{sup 8} boron atoms would be delivered per cell. Since EGF has a comparatively low molecular weight ({approximately} 6 kD), this has allowed us to construct relatively small bioconjugates containing {approximately} 900 boron atoms per EGF molecule{sup 3}, which also had high affinity for EGFR on tumor cells. In the present study, the feasibility of using EGF receptors as a potential target for therapy of gliomas was investigated by in vivo scintigraphic studies using {sup 131}I{minus} or {sup 99m}{Tc}-labeled EGF in a rat brain tumor model. Our results indicate that intratumorally delivered boron- EGF conjugates might be useful for targeting EGFR on glioma cells if the boron containing moiety of the conjugates persisted intracellularly. Further studies are required, however, to determine if this approach can be used for BNCT of the rat glioma.
Date: December 31, 1994
Creator: Capala, J.; Barth, R.F.; Adams, D.M.; Bailey, M.Q.; Soloway, A.H. & Carlsson, J.
Partner: UNT Libraries Government Documents Department

Effect of negative pions on the proliferative capacity of ascites tumor cells (lymphoma) grown in vivo

Description: We have attempted to determine the relative biological effectiveness (RBE) of negative pions in the Bragg-peak region as compared to the plateau region and to gamma rays. We irradiated LAF{sub 1} mice, bearing 5-day-old lymphoma ascites tumors, in the peak and plateau regions of a 90-MeV pion beam for 40 hours in temperature-controlled holders. The animals were then sacrificed; lymphoma cells were withdrawn and titrated into adult female LAF{sub 1} mice. The proliferative capacity of the irradiated tumor cells was evaluated after 8 weeks by observing the percentage of animals developing ascites tumors. Surviving fractions were then calculated from LD{sub 50}`s of control and irradiated animals. Radiation doses in the 50 plateau region were measured with LiF dosimeters calibrated against cobalt-60 gamma rays. We calculated peak doses from those at the plateau, using a measured average peak-to-plateau ionization ratio of 1.5. Doses in the plateau region ranged from 145 to 250 rads; doses in the peak region ranged from 220 to 380 rads. The survival curve for cells irradiated in the peak region gave a D{sub 0} of 65 {plus_minus} 15 rads. The plateau points were not reliable. A replicate experiment was performed using Co{sup 60} {gamma}-rays, yielding a survival-curve D{sub 0} of 350 {plus_minus} 50 rads. If the {gamma}-ray D{sub 0} is taken as a baseline, an RBE of 5.4 {plus_minus} 1.8 is obtained for negative pions in the peak region, based on the ratio of-peak-region D{sub 0} to Co{sup 60} D{sub 0}.
Date: March 30, 1967
Creator: Feola, J.M.; Richman, C.; Raju, M.R.; Curtis, S.B. & Lawrence, J.H.
Partner: UNT Libraries Government Documents Department

Determination of radiobiological parameters for the safe clinical application of BNCT

Description: In the present report the effects of BNCT irradiation on the skin and spinal cord of Fischer 344 rats, for known concentrations of {sup 10}B in the blood and these normal tissues, are compared with the effects of the neutron beam alone or photon irradiation. The biological effectiveness of irradiation in the presence of the capture agents BSH and BPA have been compared. Irradiations were carried out using the thermal beam of the Brookhaven Medical Research Reactor (BMRR). Therapy experiments were also carried out as part of this study, using the rat 9L-gliosarcoma cell line, in order to establish the potential therapeutic advantage that might be achieved using the above capture agents. This cell line grows as a solid tumor in vivo as well as in vitro. The implications of these findings, with respect to the clinical use of the Petten HBII based epithermal neutron beam, will be discussed.
Date: December 31, 1993
Creator: Hopewell, J. W.; Morris, G. M. & Coderre, J. A.
Partner: UNT Libraries Government Documents Department

INEL BNCT Research Program, January/February 1993

Description: This report presents summaries for two months of current research of the Idaho National Engineering Laboratory (INEL) Boron Neutron Capture Therapy (BNCT) Program. Information is presented on development and murine screening experiments of low-density lipoprotein, carboranyl alanine, and liposome boron containing compounds. Pituitary tumor cell culture studies are described. Drug stability, pharmacology and toxicity evaluation of borocaptate sodium (BSH) and boronophenylaianine (BPA) are described. Treatment protocol development via the large animal (canine) model studies and physiological response evaluation in rats are discussed. Supporting technology development and technical support activities for boron drug biochemistry and purity, analytical and measurement dosimetry, and noninvasive boron quantification activities are included for the current time period. Current publications for the two months are listed.
Date: April 1, 1993
Creator: Venhuizen, J. R.
Partner: UNT Libraries Government Documents Department

INEL BNCT Research Program, March/April 1993

Description: This report presents summaries for two months of current research of the Idaho National Engineering Laboratory Boron Neutron Capture Therapy Program. Information is presented on development and murine screening experiments of low-density lipoprotein, carboranyl alanine, and liposome boron containing compounds. Pituitary tumor cell culture studies are described. Drug stability, pharmacology and toxicity evaluation of borocaptate sodium and boronophenylalanine are described. Treatment protocol development via the large animal (canine) modal studies and physiological response evaluation in rats are discussed. Supporting technology development and technical support activities for boron drug biochemistry and purity, analytical and measurement dosimetry, and noninvasive boron quantification activities are included for the current time period. Current publications for the two months are listed.
Date: June 1, 1993
Creator: Venhuizen, J. R.
Partner: UNT Libraries Government Documents Department

Protection by WR-151327 against late-effect damage from fission-spectrum neutrons

Description: Considerable effort has been expended to develop chemical agents capable of modifying radiation-induced damage to biological systems. The authors describe here differences in the radioprotective effect of WR-151327, depending on the sex of the animal and the post-irradiation time interval considered. The greatest effect in female animals is prior to 805 days post irradiation. The greater protection in male animals is seen during the time increment following 850 days after irradiation. While it is difficult at present to ascribe these effects to a particulate model, it is suggestive that hormonal factors may play a role in aminothiol protection against radiation-induced life shortening and concomitant tumor induction in the B6CF{sub 1} hybrid mouse system. With respect to subsequent tumor induction, their preliminary findings to be published elsewhere suggest that tumors of lymphoreticular origin are the class of tumors most affected by the administration of a radioprotector prior to irradiation. 23 refs., 4 figs.
Date: January 1, 1990
Creator: Grdina, D.J. (Argonne National Lab., IL (USA) Chicago Univ., IL (USA). Dept. of Radiation and Cellular Oncology); Wright, B.J. & Carnes, B.A. (Argonne National Lab., IL (USA))
Partner: UNT Libraries Government Documents Department

Positron emission tomographic imaging of tumors using monoclonal antibodies. Progress report, November 1, 1992--October 31, 1993

Description: The overall goal of this project is to be able to combine the molecular specificity of monoclonal antibodies with the imaging advantages of positron emission tomography. During the past year, were have made progress in a number of areas. This report will focus on our studies evaluating the potential of two different methods for labeling a monoclonal antibody fragment with positron-emitting F-18 both in vitro and in athymic mice bearing subcutaneous D-54 MG human glioma xenografts. The F (a b{prime}){sub 2} fragment of Me1-14, a murine egg{sub 2a} reactive with an epitope of the tumor associated proteoglycan sulfate present in gliomas and melanomas, was used. This antibody is a particular interest because of our ongoing clinical radioimmunotherapy trails using Me1--14 that could ultimately benefit from the determination of quantitative dosimetry using monoclonal antibody PET imaging. Our results demonstrated, for the first time, that MAb fragments could be labeled with F-18 with retention of immunoreactivity and affinity. Further, they show that selective and specific tumor uptake of an F-18 labeled MAb fragment can be achieved in a xenograft model in a time frame compatible with the short half life of this nuclide.
Date: July 29, 1993
Creator: Zalutsky, M. R.
Partner: UNT Libraries Government Documents Department

In vivo studies in NCT with a boronated porphyrin and tumor growth delay as an end point

Description: The robust carrying capacity of the porphyrin molecule and its propensity for localizing in tumor justified the synthesizing of a porphyrin labeled with boron for use in BNCT. However, problems associated with poor solubility impeded the utility of the molecule. Until BOPP was synthesized porphyrins were promising, but impractical. After in vitro experiments had demonstrated the biological efficacy of BOPP and had confirmed its intracellular localizing ability in vivo studies were carried out using mice. Irradiation of KHJJ murine mammary carcinoma to the TCD{sub 50} in a single fraction was precluded since this whole body dose is lethal. This problem was overcome by the use of radiation. BOPP was administered either as three 0.5 ml injections per day over two days or by continuous i.v. infusion, 2 ml per day over three days for a total dose of about 42 {mu}g {sup 10}B/gbw. Boron-10 distribution in the tumor at the time of irradiation was {approximately}20 {mu}g.
Date: December 31, 1992
Creator: Laster, B. H.; Kahl, S. B.; Warkentien, L. & Bond, V. P.
Partner: UNT Libraries Government Documents Department

Synthesis and evaluation of boron compounds for neutron capture therapy of malignant brain tumors. Technical progress report No. 1, May 1, 1990--January 31, 1991

Description: Boron neutron capture therapy offers the potentiality for treating brain tumors currently resistant to treatment. The success of this form of therapy is directly dependent upon the delivery of sufficient numbers of thermal-neutrons to tumor cells which possess high concentrations of B-10. The objective of this project is to develop chemical methodology to synthesize boron-containing compounds with the potential for becoming incorporated into rapidly-dividing malignant brain tumor cells and excluded from normal components of the brain and surrounding tissues, to develope biological methods for assessing the potential of the compound by use of cell culture or intratumoral injection, to develop analytical methodology for measuring boron in cells and tissue using direct current plasma atomic emission spectroscopy (DCP-AES) and alpha track autoradiography, to develop biochemical and HPLC procedures for evaluating compound uptake and tissue half-life, and to develop procedures required to assess both in vitro and vivo efficacy of BNCT with selected compounds.
Date: December 31, 1990
Creator: Soloway, A. H. & Barth, R. F.
Partner: UNT Libraries Government Documents Department

INEL BNCT Research Program annual report, 1992

Description: This report is a summary of the progress and research produced for the Idaho National Engineering Laboratory Boron Neutron Capture Therapy (BNCT) Research Program for calendar year 1992. Contributions from all the principal investigators about their individual projects are included, specifically, chemistry (pituitary tumor targeting compounds, boron drug development including liposomes, lipoproteins, and carboranylalanine derivatives), pharmacology (murine screenings, toxicity testing, inductively coupled plasma-atomic emission spectroscopy (ICP-AES) analysis of biological samples), physics (radiation dosimetry software, neutron beam and filter design, neutron beam measurement dosimetry), and radiation biology (small and large animal models tissue studies and efficacy studies). Information on the potential toxicity of borocaptate sodium and boronophenylalanine is presented, results of 21 spontaneous-tumor-bearing dogs that have been treated with BNCT at the Brookhaven National Laboratory (BNL) Medical Research Reactor (BMRR) are discussed, and predictions for an epithermal-neutron beam at the Georgia Tech Research Reactor (GTRR) are shown. Cellular-level boron detection and localization by secondary ion mass spectrometry, sputter-initiated resonance ionization spectroscopy, low atomization resonance ionization spectroscopy, and alpha track are presented. Boron detection by ICP-AES is discussed in detail. Several boron carrying drugs exhibiting good tumor uptake are described. Significant progress in the potential of treating pituitary tumors with BNCT is presented. Measurement of the epithermal-neutron flux at BNL and comparison to predictions are shown. Calculations comparing the GTRR and BMRR epithermal-neutron beams are also presented. Individual progress reports described herein are separately abstracted and indexed for the database.
Date: May 1, 1993
Creator: Venhuizen, J. R.
Partner: UNT Libraries Government Documents Department

Research in radiobiology: Final report of work in progress in immunobiology of experimental host-tumor relationships

Description: Our work on the immunobiology of tumors induced in normal mice by non-ionizing radiation and chemical carcinogens has previously demonstrated a correlation between MHC molecule expression and the immunogenicity of tumors in a transplanted syngeneic host. Such that immunogenic or regressive tumors were found to demonstrate higher constitutive or inducible levels of MHC expression, while most virulent, aggressive tumors exhibited a low level of MHC Class I expression. We attributed much of the control of MHC molecule expression by antigen-bearing tumors and normal cells to the immunological status of the host since the host must provide the appropriate stimulus to enhance MHC antigen expression by the invading tumor. Our results with UVR-induced tumors suggested that a significant role is played by the T-cell lymphokine, {gamma}-interferon ({gamma}IFN), in the modulation of MHC molecule expression in vivo. Virulent tumors, induced by boneseeking radionuclides, may be refractory to {gamma}IFN stimulation of MHC molecule expression. It is also possible that certain tumors might be fully responsive to the Class I modulatory influences by {gamma}IFN, but exhibit a reduced capacity to stimulate the synthesis of this lymphokine by host T cells. We present experiments designed to : Describe the virulence, latency period, and transplantation characteristics of {sup 238}PU, {sup 24l}Am, and {sup 228}Th tumors arising as osteogenic sarcomas and hepatic carcinomas, to determine the relationship between inducible expression of MHC Class I molecules by {gamma}IFN and in vivo immunogenicity of these radioisotype-induced tumors, and to elucidate any molecular mechanisms responsible for a lack of responsiveness to a {gamma}IFN failure by the host to induce host {gamma}IFN production.
Date: March 15, 1993
Partner: UNT Libraries Government Documents Department

Radiogenic neoplasia in thyroid and mammary clonogens. Progress report, January 1, 1990--December 31, 1992

Description: We have developed rat thyroid and mammary clonogen transplantation systems for the study of radiogenic cancer induction at the target cell level in vivo. The epithelial cell populations of both glands contain small subpopulations of cells which are capable of giving rise to monoclonal glandular structures when transplanted and stimulated with appropriate hormones. Previous results indicated that these clonogens are the precursor cells of radiogenic cancer, and that initiation, is common event at the clonegenic cell level. Detailed information on the physiologic control of clonogen proliferation, differentiation, and total numbers is thus essential to an understanding of the carcinogenic process. We report here studies on investigations on the relationships between grafted thyroid cell number and the rapidity and degree of reestablishment of the thyroid-hypothalamus-pituitary feedback axis in thyroidectomized rats maintained on a normal diet or an iodine deficient diet; studies of the persistence of, and the differentiation potential and functional characteristics of, the TSH-(thyrotropin-) responsive sub- population of clonogens during goitrogenesis, the plateau-phase of goiter growth, and goiter involution; studies of changes in the size of the clonogen sub-population during goitrogenesis, goiter involution and the response to goitrogen rechallenge; and a large carcinogenesis experiment on the nature of the grafted thyroid cell number-dependent suppression of promotion/progression to neoplasia in grafts of radiation-initiated thyroid cells. Data from these studies will be used in the design of future carcinogenesis experiments on neoplastic initiation by high and low LET radiations and on cell interactions during the neoplastic process.
Date: May 20, 1992
Creator: Clifton, K. H.
Partner: UNT Libraries Government Documents Department

[A clinical trial of neutron capture therapy for brain tumors]. Technical progress report, 1990

Description: This document briefly describes recent advances in the author`s laboratory. Topics described include neutron beam design, high- resolution autoradiography, boronated phenylalanine (BPA) distribution and survival studies in glioma bearing mice, computer- aided treatment planning, prompt gamma boron 10 analysis facility at MITI-II, non-rodent BPA toxicity studies, and preparations for clinical studies.
Date: December 31, 1990
Creator: Zamenhof, R. G.
Partner: UNT Libraries Government Documents Department

[Initiation, promotion, initiation experiments with radon and cigarette smoke: Lung tumors in rats]. Progress report

Description: During the past several years, the authors have made considerable progress in modeling carcinogenesis in general, and in modeling radiation carcinogenesis, in particular. They present an overview of their progress in developing stochastic carcinogenesis models and applying them to experimental and epidemiologic data sets. Traditionally, cancer models have been used for the analysis of incidence (or prevalence) data in epidemiology and time to tumor data in experimental studies. The relevant quantities for the analysis of these data are the hazard function and the probability of tumor. The derivation of these quantities is briefly described here. More recently, the authors began to use these models for the analysis of data on intermediate lesions on the pathway to cancer. Such data are available in experimental carcinogenesis studies, in particular in initiation and promotion studies on the mouse skin and the rat liver. If however, quantitative information on intermediate lesions on the pathway to lung cancer were to be come available at some future date, the methods that they have developed for the analysis of initiation-promotion experiments could easily be applied to the analysis of these lesions. The mathematical derivations here are couched in terms of a particular two-mutation model of carcinogenesis. Extension to models postulating more than two mutations is not always straightforward.
Date: October 1, 1994
Creator: Moolgavkar, S. H.
Partner: UNT Libraries Government Documents Department

Improved radioimmunotherapy of hematologic malignancies. Progress report, November 1, 1993--October 31, 1994

Description: This report summaries progress made during the time interval between November 1, 1993 and October 31, 1994 and briefly describes studies on the metabolism of antibodies targeting B cell antigens, retention of labeled antibodies by human B cell lymphocytes, and tissue distribution of Chloramine T and tyramine cellobiose labeled antibodies in mice harboring a human erythroleukemia tumor transplant.
Date: August 4, 1994
Creator: Press, O. W.
Partner: UNT Libraries Government Documents Department

(Accumulation of methyl-deficient rat liver messenger ribonucleic acid on ethionine administration). Progress report. [Methyltransferase activity in Ehrlich ascites tumor cells and effects of phorbol ester on methyltransferase activity]

Description: Enzyme fractions were isolated from Ehrlich ascites cells which introduced methyl groups into methyl deficient rat liver mRNA and unmethylated vaccinia mRNA. The methyl groups were incorporated at the 5' end into cap 1 structures by the viral enzyme, whereas both cap 0 and cap 1 structures were formed by the Ehrlich ascites cell enzymes. Preliminary results indicate the presence of adenine N/sup 6/-methyltransferase activity in Ehrlich ascites cells. These results indicate that mRNA deficient in 5'-cap methylation and in internal methylation of adenine accumulated in rats on exposure to ethionine. The methyl-deficient mRNA isolated from the liver of ethionine-fed rats differed in its translational properties from mRNA isolated from control animals. Preliminary experiments indicate that single topical application of 17n moles of TPA to mouse skin altered tRNA methyltransferases. The extent of methylation was increased over 2-fold in mouse skin treated with TPA for 48 hours. These changes have been observed as early as 12 hours following TPA treatment. In contrast, the application of initiating dose of DMBA had no effect on these enzymes. It should be emphasized that the changes in tRNA methyltransferases produced by TPA are not merely an increase of the concentration of the enzyme, rather that they represent alterations of specificity of a battery of enzymes. In turn the change in enzyme specificity can produce alterations in the structure of tRNA. (ERB)
Date: January 1, 1980
Creator: Borek, E.
Partner: UNT Libraries Government Documents Department

Characterization of cell suspensions from solid tumors

Description: The desirable features of cells in suspension will necessarily be dependent upon the use for which the cells were prepared. Adequate cell yield or recovery is defined by the measurement to be performed. Retention of cellular morphology is important for microscopic identification of cell types in a heterogenous cell suspension, and may be used to determine whether the cells in suspension are representative of those in the tumor in situ. Different dispersal protocols may yield cells with different degrees of clonogenicity, as well as altered biochemical features, such as loss of cellular proteins, surface antigens, nucleotide pools, etc. The quality of the cell suspension can be judged by the degree of cell clumping and level of cellular debris, both of which impact on flow cytometric measurements and studies in which the number of cells be known accurately. Finally, if the data measured on the cells in suspension are to be extrapolated to phenomena occurring in the tumor in situ, it is desirable that the cells in suspension are representative of those in the solid tumor in vivo. This report compares characteristics of tumor cell suspensions obtained by different types of selected disaggregation methods. 33 refs., 2 figs., 4 tabs.
Date: July 10, 1985
Creator: Pallavicini, M.
Partner: UNT Libraries Government Documents Department