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open access

Lymphocytes from wasted mice express enhanced spontaneous and {gamma}-ray-induced apoptosis

Description: Mice bearing the autosomal recessive mutation wasted (wst/wst) display a disease pattern including faulty repair of DNA damage in lymphocytes after radiation exposure, neurologic abnormalities, and immunodeficiency. Many of the features of this mouse model have suggested a premature or increased spontaneous frequency of apoptosis in thymocytes; past work has shown an inability to establish cultured T cell lines, an abnormally high death rate of stimulated T cells in culture, and an increased se… more
Date: September 1, 1993
Creator: Woloschak, Gayle E.; Chang-Liu, Chin-Mei; Chung, Jen & Libertin, Claudia R.
Partner: UNT Libraries Government Documents Department
open access

Expression of cytoskeletal and matrix genes following exposure to ionizing radiation: Dose-rate effects and protein synthesis requirements

Description: Experiments examined the effects of radiation dose-rate and protein synthesis inhibition expression of cytoskeletal and matrix elements in Syrian hamster embryo cells. Results demonstrated little effect of dose-rate for neutrons when comparing expression of {alpha}-tubulin and fibronectin genes. Cycloheximide repressed accumulation of {alpha}-tubulin-mRNA following exposure to high dose-rate neutrons or {gamma} rays. Cycloheximide did not affect accumulation of actin mRNA. Cycloheximide abrogat… more
Date: June 1, 1995
Creator: Woloschak, Gayle E.; Felcher, Paolo & Chang-Liu, Chin-Mei
Partner: UNT Libraries Government Documents Department
open access

Expression of cytoskeletal and matrix genes following exposure to ionizing radiation: Dose-rate effects and protein synthesis requirements

Description: Experiments were designed to examine the effects Of radiation dose-rate and of the protein synthesis inhibitor cycloheximide on expression of cytoskeletal elements ({gamma}- and {beta}-actin and {alpha}-tubulin) and matrix elements (fibronectin) in Syrian hamster embryo cells. Past work from our laboratory had already demonstrated optimum time points and doses for examination of radiation effects on accumulation of specific transcripts. Our results here demonstrated little effect of dose-rate f… more
Date: May 1, 1994
Creator: Woloschak, G. E.; Felcher, P. & Chang-Liu, Chin-Mei
Partner: UNT Libraries Government Documents Department
open access

Proteomic Profiling of Mesenchymal Stem Cell Responses to Mechanical Strain and TGF-B1

Description: Mesenchymal stem cells (MSCs) are a potential source of smooth muscle cells (SMCs) for constructing tissue-engineered vascular grafts. However, the details of how specific combinations of vascular microenvironmental factors regulate MSCs are not well understood. Previous studies have suggested that both mechanical stimulation with uniaxial cyclic strain and chemical stimulation with transforming growth factor {beta}1 (TGF-{beta}1) can induce smooth muscle markers in MSCs. In this study, we inve… more
Date: October 12, 2009
Creator: Kurpinski, Kyle; Chu, Julia; Wang, Daojing & Li, Song
Partner: UNT Libraries Government Documents Department
open access

Combined effects of ionizing radiation and cycloheximide on gene expression

Description: Experiments were done to determine the effects of ionizing radiation exposure on expression of genes following exposure of Syrian hamster embryo (SHE) cells to the protein synthesis inhibitor cycloheximide (including such genes as {beta}-actin, c-fos, H4-histone, c-myc, c-jun, Rb, and p53). Results revealed that when ionizing radiations (either fission-spectrum neutrons or {gamma}-rays) were administered 15 min following the cycloheximide treatment of SHE cells, the radiation exposure reduced c… more
Date: November 1, 1993
Creator: Woloschak, G. E.; Felcher, P. & Chang-Liu, Chin-Mei
Partner: UNT Libraries Government Documents Department
open access

Expression of cytoskeletal and matrix genes following exposure to ionizing radiation: Dose-rate effects and protein synthesis requirements

Description: Experiments were designed to examine the effects of radiation dose-rate and of the protein synthesis inhibitor cycloheximide on expression of cytoskeletal elements ({gamma}- and {beta}-actin and {alpha}-tubulin) and matrix elements (fibronectin) in Syrian hamster embryo cells. Results demonstrated little effect of dose-rate for JANUS fission-spectrum neutrons when comparing expression of either a-tubulin or fibronectin genes. Past work had already documented similar results for expression of ac… more
Date: December 31, 1993
Creator: Woloschak, G. E.; Felcher, P. & Chang-Liu, Chin-Mei
Partner: UNT Libraries Government Documents Department
open access

Role of protein synthesis in the repair of sublethal x-ray damage in a mutant Chinese hamster ovary cell line

Description: A temperature-sensitive mutant for protein synthesis, CHO-TSH1, has been compared to the wild-type cell, CHO-sC1, in single- and split-radiation-dose schemes. When the exponentially growing TS mutant and the wild-type cells were treated at 40/sub 0/C for up to 2 hrs prior to graded doses of x rays, the survival curves were identical and were the same as those obtained without heat treatment. If the cultures were incubated at 40/sup 0/C for 2 hrs before a first dose and maintained at 40/sup 0/C … more
Date: April 1, 1985
Creator: Yezzi, M.J.
Partner: UNT Libraries Government Documents Department
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