1,639 Matching Results

Search Results

Advanced search parameters have been applied.
in subjects: "Biochemistry."  
Results: 1 - 24 next
open access

Rapid inactivation of SARS-like coronaviruses.

Description: Chemical disinfection and inactivation of viruses is largely understudied, but is very important especially in the case of highly infectious viruses. The purpose of this LDRD was to determine the efficacy of the Sandia National Laboratories developed decontamination formulations against Bovine Coronavirus (BCV) as a surrogate for the coronavirus that causes Severe Acute Respiratory Syndrome (SARS) in humans. The outbreak of SARS in late 2002 resulted from a highly infectious virus that was ableā€¦ more
Date: March 1, 2004
Creator: Kapil, Sanjay (Kansas State University, Manhattan, KS); Oberst, R. D. (Kansas State University, Manhattan, KS); Bieker, Jill Marie; Tucker, Mark David; Souza, Caroline Ann & Williams, Cecelia Victoria
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Analysis of Biological Materials Using a Nuclear Microprobe

Description: The use of nuclear microprobe techniques including: Particle induced x-ray emission (PIXE) and Rutherford backscattering spectrometry (RBS) for elemental analysis and quantitative elemental imaging of biological samples is especially useful in biological and biomedical research because of its high sensitivity for physiologically important trace elements or toxic heavy metals. The nuclear microprobe of the Ion Beam Modification and Analysis Laboratory (IBMAL) has been used to study the enhancemeā€¦ more
Date: December 2014
Creator: Mulware, Stephen Juma
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Purification and Studies of Methylglyoxal Reductase from Sheep Liver

Description: The objectives of these investigations were (1) the purification of MG reductase from sheep liver and (2) studies of some of its characteristics. MG reductase was purified 40 fold and showed a single band on SDS-PAGE. Molecular weight estimations with SDS-PAGE showed a molecular weight of 44,000; although gel filtration with Sephadex G-150 gave a molecular weight of 87,000 indicating that the enzyme might be a dimer. The Km for MG is 1.42 mM and for NADH it is 0.04 mM. The pH optimum for the puā€¦ more
Date: May 1983
Creator: Lambert, Patricia A.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Computational Studies on Group 14 Elements (C, Si and Ge) in Organometallic and Biological Compounds.

Description: A series of computational studies were carried out on Group 14 (C, Si and Ge) elements in organometallic and biological compounds. Theoretical studies on classical and H-bridged A3H3+ (A=C, Si and Ge) as p ligands with different organometallic fragments at B3LYP and B3P86 level reveal a reverse charge transfer from ligand to metal in Si and Ge complexes whereas in C complexes there is a small charge transfer from metal to ligand. The H-bridged complexes are more stable than the complexes baseā€¦ more
Date: May 2007
Creator: Yu, Liwen
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Viral RNA testing and automation on the bead-based CBNE detection microsystem.

Description: We developed prototype chemistry for nucleic acid hybridization on our bead-based diagnostics platform and we established an automatable bead handling protocol capable of 50 part-per-billion (ppb) sensitivity. We are working towards a platform capable of parallel, rapid (10 minute), raw sample testing for orthogonal (in this case nucleic acid and immunoassays) identification of biological (and other) threats in a single sensor microsystem. In this LDRD we developed the nucleic acid chemistry reā€¦ more
Date: September 1, 2008
Creator: Galambos, Paul C.; Bourdon, Christopher Jay; Farrell, Cara M.; Rossito, Paul (University of California at Davis); McClain, Jaime L.; Derzon, Mark Steven et al.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

A survey of Asian life scientists :the state of biosciences, laboratory biosecurity, and biosafety in Asia.

Description: Over 300 Asian life scientists were surveyed to provide insight into work with infectious agents. This report provides the reader with a more complete understanding of the current practices employed to study infectious agents by laboratories located in Asian countries--segmented by level of biotechnology sophistication. The respondents have a variety of research objectives and study over 60 different pathogens and toxins. Many of the respondents indicated that their work was hampered by lack ofā€¦ more
Date: February 1, 2006
Creator: Gaudioso, Jennifer Marie
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Argonne National Laboratory Biology Division Quarterly Report: August to October, 1946

Description: From introductory paragraph: "This is the first quarterly report of the Biology Division of the Argonne National Laboratory. During the three-month period covered by this report, a large share of the time of many investigators has been spent in collating work done under the Biology Section of the Metallurgical laboratory for publication in the Plutonium Project Record. The large amount of work done under wartime pressure has made this, as in other divisions and projects, an important part of thā€¦ more
Date: November 21, 1946
Creator: Bruss, Austin M.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Ultramicro Methods in Biochemistry: [Part] 1. General Consideration, [Part] 2. Procedures for the Determination of Serum Bilirubin

Description: From Introduction: "The purpose of this paper is first to present a positive, but initial, approach that has been taken to meet these requirements, and second to report in detail the ultramicro method developed and evaluated for determining serum bilirubin. Subsequent publications will set forth the techniques finalized for other biochemical constituents."
Date: October 1962
Creator: Van Stewart, E.; Puckett, Charles R. & Wood, Agnes
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Evidence for a Role of the Multifunctional Calcium/Calmodulin-Dependent Protein Kinase II in Insulin Secretion

Description: Calcium/calmodulin-dependent protein kinase II (CaM kinase II) is demonstrated to exist in the Ɵ-cell and immunopecipitation. Glucose and potassium significantly stimulate the rapid autophosphorylation of CaM kinase II and proportionally induce autonomous activity of the kinase in a dose-dependent manner that parallels insulin secretion. The activation of CaM kinase II, alloxan, KN-62 and KN-93, suggest that the enzyme is an integral component of insulin secretion and/or related processes in thā€¦ more
Date: December 1993
Creator: Wenham, Robert M. (Robert Michael)
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Studies on the Structure and Function of Glucosephosphate Isomerases: Chemical Modifications, Chemical Cleavages and Structural Analyses

Description: Human glucosephosphate isomerase was subjected to a series of chemical modifications aimed at identifying residues essential for catalytic activity. Specific lysyl, arginyl, tryptophanyl and histidyl residues were found to react stoichiometrically with pyridoxal-5'-phosphate-NaBH4, 2,3-butadione, N-bromosuccinimide and N-bromoacetylethanolamine phosphate, respectively.
Date: December 1981
Creator: Lu, Hsieng Sen
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

A Proposed Molecular Mechanism for the Activation of a Calcium/Phospholipid-Dependent Protein Kinase in P1798 Lymphosarcoma

Description: Calcium/phospholipid-dependent protein kinase (PKC) was purified from P1798 lymphosarcoma. It was demonstrated that uncomplexed calcium and uncomplexed phosphatidylserine are the activators, that the activation of PKC requires that calcium bind first, that the activation of PKC requires that calcium bind first, that high calcium concentrations inhibit PKC activation, and that calcium inhibition can be overcome by phosphatidylserine.
Date: May 1987
Creator: Elson, James L.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Two legume fatty acid amide hydrolase isoforms with distinct preferences for microbial- and plant-derived acylamides

Description: Article describes how analysis of FAAHs from multiple angiosperm species, which revealed two conserved phylogenetic groups that differed in key conserved residues in the substrate binding pocket. The authors combined computational and biochemical approaches to compare the structural and enzymatic properties of two FAAH isoforms in the legume Medicago truncatula designated MtFAAH1 MtFAAH2a.
Date: May 9, 2023
Creator: Arias-Gaguancela, Omar; Herrell, Emily; Aziz, Mina & Chapman, Kent Dean
Item Type: Refine your search to only Article
Partner: UNT College of Science

Proteomic-Based Assessment of Estrogenic Endocrine Disruption in Hyalella azteca

Description: In our studies, we used the environmentally important crustacean Hyalella azteca (H. azteca) as an invertebrate model and 17Ī²-estradiol (E2) as a representative of environmental estrogenic endocrine disrupting compounds (EDCs) for proteomics-based investigations of endocrine disruptions in an aquatic ecosystem. Using liquid chromatography coupled with tandem mass spectrometry, our investigation focused for the first time on the recognition of biological and molecular events affected by E2 exposā€¦ more
This item is restricted from view until January 1, 2029.
Date: December 2023
Creator: Prokai, Marcel Laszlo
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Complex Polymers of ADP-Ribose Occur in Vitro and in Vivo

Description: The work presented here included the development of a highly sensitive method to estimate the size and complexity of poly(ADP-ribose). This involved radiolabeling of the precursor pools, purification of polymers using a boronate resin, polymer fractionation according to size by molecular sieve chromatography and analysis of polymer complexity by enzymatic digestion to nucleotides which were quantified by strong anion exchange chromatography.
Date: May 1985
Creator: Alvarez-Gonzalez, Rafael
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

High Resolution Fractionation, Characterization, and Studies of ADP-Ribose Polymers

Description: A method for the high resolution fractionation of ADP-ribose polymers has been developed that allows isolation of highly purified polymers of defined size and branching fequency. The key features of the method are purification using dihydroxyboronyl-BioRex 70 and fractionation by anion exchange HPLC.
Date: August 1992
Creator: Kiehlbauch, Charles C. (Charles Coffey)
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Annual report and summaries of FY 1993 activities: Division of Energy Biosciences

Description: The mission of the Energy Biosciences program is to generate fundamental information about plants and non-health related microorganisms that will constitute the base for new biotechnologies as well as supply information to improve usages of such organisms in their current form. The collective aims are totally consistent with the Department of Energy`s objectives of developing alternate energy sources, replacements for otherwise fossil energy derived products and providing critical fundamental iā€¦ more
Date: September 1, 1993
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
Back to Top of Screen