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Oxidation of Aromatic Compounds by Bacteria

Description: From Introduction: "The material in this paper is about central paths and how bacterial enzymes manipulate and cleave the aromatic ring with the formation of hydroxylated aromatic ring with the formation
Date: 1962
Creator: Rogoff, Martin H. & Wender, Irving
Partner: UNT Libraries Government Documents Department

Investigation of the Effect of Type 2 Diabetes Mellitus on Subgingival Plaque Microbiota by High-Throughput 16S rDNA Pyrosequencing

Description: Article discussing the bacterial composition of subgingival plaque among diabetic and non-diabetic subjects to determine the effect that diabetes mellitus has on dental health.
Date: April 22, 2013
Creator: Zhou, Mi; Rong, Ruichen; Munro, Daniel; Zhu, Chunxia; Gao, Xiang; Zhang, Qi et al.
Partner: UNT College of Arts and Sciences

A Bacteriological Survey of a Freshwater Reservoir

Description: In this study organisms that can be subcultured from lake water, using a prescribed procedure, limit, to an extent, the population, or portions of the population, that can be monitored. In essence, what is taking place is that a set of conditions is set forth and a study is made of the bacteria that will grow under these prescribed conditions.
Date: January 1968
Creator: Stiles, John Clayborn
Partner: UNT Libraries

Obligately Thermophilic Nitrogen-Fixation in Some Soil Bacteria

Description: In the work presented here, it is claimed that bacteria have been isolated which are capable of growth at high temperatures utilizing molecular nitrogen as their sole nitrogen source. Soil bacteria were isolated which grew at 55 C in nitrogen-free media. They were found to be obligatory thermophiles in nitrogen-free media and facultative thermophiles in media containing organically bound nitrogen.
Date: August 1971
Creator: Milam, Mary
Partner: UNT Libraries

The Growth of Azotobacter vinelandii on p-Hydroxybenzoic Acid from Soil Medium

Description: The purpose of this study was to search for the substrates utilized by Azotobacter vinelandii in dialysed soil media. Also, we sought to determine the relationship between these substrates and the growth and morphological variations of A. vinelandii. p-Hydroxybenzoic acid was shown to be used as the carbon and energy source by A. vinelandii in dialysed soil medium. The amount of this compound in the soil dialysed soil medium ranged from 14 to 21 micrograms per gram of soil. In a dialysed soil medium, p-hydroxybenzoic acid induced A. vinelandii to form minute bodies, similar to the filtrable forms reported by Gonzalez and Vela, although no growth of minute bodies was detected.
Date: August 1982
Creator: Wu, Fang Jy
Partner: UNT Libraries

The Antibiotic Activities of Some Members of the Cactaceae Family

Description: This problem has been concerned with, first, the collection of sixteen species of plants belonging to Cactaceae family; second, the drying of these and the extraction of the oleoresins thereof; third, the determination of the extent to which these substances inhibit the growth of ten gram-positive and ten gram-negative bacterial organisms; and fourth, a determination of the possible utilization of these extracts a prophylactic or chemotherapeutic agents.
Date: 1951
Creator: Gilmore, Derward E.
Partner: UNT Libraries

Comparative Biochemistry and Evolution of Aspartate Transcarbamoylase from Diverse Bacteria

Description: Aspartate transcarbamoylase (ATCase) catalyzes the first committed step in pyrimidine biosynthesis. Bacterial ATCases are divided into three classes, A, B and C. Class A ATCases are largest at 450-500, are. dodecamers and represented by Pseudomonas ATCase. The overlapping pyrBC' genes encode the Pseudomonases ATCase, which is active only as a 480 kDa dodecamer and requires an inactive pyrC'-encoded DHOase for ATCase activity. ATCase has been studied in two non-pathogenic members of Mycobacterium, M. smegmatis and M. phlei. Their ATCases are dodecamers of molecular weight 480 kDa, composed of six PyrB and six PyrC polypeptides. Unlike the Pseudomonas ATCase, the PyrC polypeptide in these mycobacteria encodes an active DHOase. Moreover, the ATCase: DHOase complex in M. smegmatis is active both as the native 480 kDa and as a 390 kDa complex. The latter lacks two PyrC polypeptides yet retains ATCase activity. The ATCase from M. phlei is similar, except that it is active as the native 480 kDa form but also as 450,410 and 380 kDa forms. These complexes lack one, two, and three PyrC polypeptides, respectively. By contrast,.ATCases from pathogenic mycobacteria are active only at 480 kDa. Mycobacterial ATCases contain active DHOases and accordingly. are placed in class A1 . The class A1 ATCases contain active DHOases while class A2 ATCases contain inactive DHOases. ATCase has also been purified from Burkholderia cepacia and from an E. coli strain in which the cloned pyrB of B. cepacia was expressed. The B. cepacia ATCase has a molecular mass of 550 kDa, with two different polypeptides, PyrB (52 kDa) and PyrC of (39 kDa). The enzyme is active both as the native enzyme at 550 kDa and as smaller molecular forms including 240 kDa and 165 kDa. The ATCase synthesized by the cloned pyrB gene has a molecular weight of 165 kDa composed ...
Date: May 1999
Creator: Hooshdaran, Massoumeh Ziba
Partner: UNT Libraries

Distribution of a Novel Gram Negative, Capsule-Forming Bacterium

Description: A novel Gram negative, capsule-forming bacterium was previously isolated in Dr. G. Roland Vela's laboratory. The distribution of this bacterium in soils from various locations was investigated. Soil samples from 188 locations around the world were examined. Isolates of the bacterium were obtained from 50 of these soils, with 48 of the isolates found in soils from the southwestern United States and northern Mexico. This suggests that this region is the natural habitat of the bacterium. The other two isolates were obtained from Madrid, Spain and Taipei, Taiwan. None were found in soils from South America or Australia. A lack of variation in morphology and physiological properties in the isolates suggests that a homogeneous population exists, even from widespread geographical locations.
Date: December 1997
Creator: Hughes, Roxana Bejarano
Partner: UNT Libraries

Adenylate Energy Charge Determinations of Soil Bacteria Grown in Soil Extract Medium

Description: The adenylate energy charge values of twenty bacteria isolated from soil and cultured in a medium consisting of soil and distilled water were determined by the luciferin-luciferase bioluminescense method. The purpose of this study was to examine the growth and energy charge values of these organisms in soil extract medium, and to determine what effect the addition of glucose has on their energy charge values. Three of the organisms employed in this study showed energy charge values similar to those reported for bacteria grown in enriched media. The remainder of the isolates demonstrated low energy charge values, and scant growth in the soil medium.
Date: August 1988
Creator: Rodriguez, Luis A. (Luis Antonio)
Partner: UNT Libraries

Isolation and Characterization of a New Capsule-Forming Bacterium

Description: A unique, previously undescribed Gram-negative bacterium was isolated from several soils in Texas and extensively characterized in this study. The cells measured 1-2 by 4-6 μm. The distinguishing characteristic of the bacterium is the extraordinary capsular material which surrounds the cells. The new isolates are aerobic, mesophilic, non motile and have the ability to utilize a variety of organic compounds as the sole source of carbon and energy. The organism grows optimally at 30° C and the optimal pH lies between 7.0-8.0. The isolates produce catalase but oxidase is not produced. They do not produce indole or hydrogen sulfide. The organism can hydrolyze gelatin and Tween 80 but not starch, esculin and casein. The major cellular fatty acid is anteiso 15:0. The guanine and cytosine content is 58-62 mole%. The organism's taxonomic position was further established by specific gene probes, 16S rRNA homology, DNA homology and "ribotyping." These data showed that it was most closely related to members of the genus Paenibacillus, although somewhat divergent from other species classified in this genus. After careful evaluation of the results obtained during this study, it is proposed that this unique bacterium be named Paenibacillus velasolus sp. nov.
Date: May 1999
Creator: Thongmee, Acharawan
Partner: UNT Libraries

Comparison of Aspartate Transcarbamoylase and Pyrimidine Salvage in Sporosarcina urea, Sprolactobacillus inulinus, Lactobacillus fermentum, and Micrococcus luteus

Description: The enzyme that catalyzes the committed step in pyrimidine biosynthesis, aspartate transcarbamoylase, has been compared in selected endospore-forming organisms and in morphologically similar control organisms. The ATCases and pyrimidine salvage from Sporosarcina ureae, Sporolactobacillus inulinus, Lactobacillus fermentum, and Micrococcus luteus were compared to those of Bacillus subtilis. While the ATCases from Sporosarcina ureae, Sporolactobacillus inulinus, and L. fermentum were found to exhibit characteristics to that of Bacillus with respect to molecular weight and kinetics, M. luteus ATCase was larger at approximately 480 kDa. Furthermore, pyrimidine salvage in Sporosarcina ureae and M. luteus was identical to those of B. subtilis, while pyrimidine salvage of Sporolactobacillus inulinus and L. fermentum resembled that of the pseudomonads.
Date: August 1994
Creator: Barron, Vincent N. (Vincent Neal)
Partner: UNT Libraries

Greengenes: Chimera-checked 16S rRNA gene database and workbenchcompatible in ARB

Description: A 16S rRNA gene database (http://greengenes.lbl.gov) addresses limitations of public repositories by providing chimera-screening, standard alignments and taxonomic classification using multiple published taxonomies. It was revealed that incongruent taxonomic nomenclature exists among curators even at the phylum-level. Putative chimeras were identified in 3% of environmental sequences and 0.2% of records derived from isolates. Environmental sequences were classified into 100 phylum-level lineages within the Archaea and Bacteria.
Date: February 1, 2006
Creator: DeSantis, T.Z.; Hugenholtz, P.; Larsen, N.; Rojas, M.; Brodie,E.L; Keller, K. et al.
Partner: UNT Libraries Government Documents Department

Heat Resistance in Vegetative Cells and Cysts of Azotobacter

Description: The purpose of the current study is to determine something of the nature of the heat resistance in Azotobacter, if in fact this is found to exist. An attempt is made to determine the specific physiological state associated with heat resistance as well as to resolve this resistance quantitatively.
Date: August 1969
Creator: Rosenthal, Raoul S.
Partner: UNT Libraries

Studies on the Nutrition of Rhodospirillum Rubrum

Description: The purpose of this study has been to examine the nutritional requirements of Rhodospirillum rubrum and, on the basis of the results, develop a culture medium which could be use to promote more rapid and abundant growth facilitating the laboratory cultivation and observations of the properties so exhibited.
Date: August 1957
Creator: Wentworth, Margaret Earl
Partner: UNT Libraries

Isolation and Partial Characterization of Carotenoid Pigments of Mycobacterium Rhodochrous

Description: It was the purpose of this investigation to isolate and characterize the pigments of Mycobacterium rhodochrous by partition behavior, chromatographic data, and absorption spectra. In addition, it was the purpose to determine whether or not the type of pigments found in M. rhodochrous is typical of those found in other mycobacteria.
Date: August 1971
Creator: Jorgensen, Joyce A.
Partner: UNT Libraries