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Interactions of N-Acylethanolamine Metabolism and Abscisic Acid Signaling in Arabidopsis Thaliana Seedlings

Description: N-Acylethanolamines (NAEs) are endogenous plant lipids hydrolyzed by fatty acid amide hydrolase (FAAH). When wildtype Arabidopsis thaliana seeds were germinated and grown in exogenous NAE 12:0 (35 µM and above), growth was severely reduced in a concentration dependent manner. Wildtype A. thaliana seeds sown on exogenous abscisic acid (ABA) exhibited similar growth reduction to that seen with NAE treatment. AtFAAH knockouts grew and developed similarly to WT, but AtFAAH overexpressor lines sh… more
Date: August 2010
Creator: Cotter, Matthew Q.
Partner: UNT Libraries
open access

A regulatory role for N-acylethanolamine metabolism in Arabidopsis thaliana seeds and seedlings.

Description: N-Acylethanolamines (NAEs) are bioactive acylamides that are present in a wide range of organisms. Because NAE levels in seeds decline during imbibition similar to ABA, a physiological role was predicted for these metabolites in Arabidopsis thaliana seed germination and seedling development. There is also a corresponding increase of AtFAAH (fatty acid amide hydrolase), transcript levels and activity, which metabolizes NAE to ethanolamine and free fatty acids. Based on whole genome microarray s… more
Date: May 2009
Creator: Teaster, Neal D.
Partner: UNT Libraries
open access

Elemental Concentrations in the Seed of Mutants and Natural Variants of Arabidopsis thaliana Grown under Varying Soil Conditions

Description: Article on elemental concentrations in the seed of mutants and natural variants of Arabidopsis thaliana grown under varying soil conditions.
Date: May 2013
Creator: McDowell, Stephen C.; Akmakjian, Garo; Sladek, Chris; Mendoza-Cózatl, David; Morrissey, Joe B.; Saini, Nick et al.
Partner: UNT College of Arts and Sciences
open access

Ultra-Fast Alterations in mRNA Levels Uncover Multiple Players in Light Stress Acclimation in Plants

Description: This article contains RNA sequencing analysis of Arabidopsis thaliana plants subjected to light stress in order to identify and characterize rapid changes in the steady-state level of different transcripts in response to light stress.
Date: September 26, 2015
Creator: Suzuki, Nobuhiro; Devireddy, Amith R.; Inupakutika, Madhuri A.; Baxter, Aaron; Miller, Gad; Song, Luhua et al.
Partner: UNT College of Arts and Sciences
open access

A chemical genetic screen uncovers a small molecule enhancer of the N-acylethanolamine degrading enzyme, fatty acid amide hydrolase, in Arabidopsis

Description: This article demonstrates the feasibility of using an unbiased chemical genetic approach to identify new pharmacological tools for manipulating FAAH- and NAE-mediated physiological processes in plants.
Date: January 23, 2017
Creator: Khan, Bibi Rafeiza; Faure, Lionel; Chapman, Kent D. & Blancaflor, Elison B.
Partner: UNT College of Arts and Sciences
open access

N-Acylethanolamine (NAE) Profiles Change During Arabidopsis Thaliana Seed Germination and Seedling Growth

Description: An understanding of the potential roles as lipid mediators of a family of bioactive metabolites called N-acylethanolamines (NAEs) depends on their accurate identification and quantification. The levels of 18C unsaturated NAEs (e.g. NAE18:2, NAE 18:3, etc.) in wild-type seeds (about 2000 ng/g fw) generally decreased by about 80% during germination and post-germinative growth. In addition, results suggest NAE-degradative fatty acid amide hydrolase (FAAH) expression does not play a major role in … more
Date: August 2006
Creator: Wiant, William C.
Partner: UNT Libraries
open access

Evidence for the Involvement of Electrical, Calcium and ROS Signaling in the Systemic Regulation of Non-Photochemical Quenching and Photosynthesis

Description: Article presents study in which pulse amplitude-modulated Chl fluorescence imaging and surface electrical potential measurements accompanied by pharmacological treatments were employed to study stimuli-induced electrical signals in leaves from a broad range of plant species and in Arabidopsis thaliana mutants.
Date: February 10, 2017
Creator: Białasek, Maciej; Górecka, Magdalena; Mittler, Ron & Karpiński, Stanisław
Partner: UNT College of Arts and Sciences
open access

The Arabidopsis gene co-expression network

Description: Article presents an Arabidopsis gene co-expression network constructed using RNA-Seq datasets, which will serve as a useful resource for the Arabidopsis research community to gain insights into Arabidopsis gene interactions and functions.
Date: April 26, 2022
Creator: Burks, David J.; Sengupta, Soham; De, Ronika; Mittler, Ron & Azad, Rajeev K.
Partner: UNT College of Science
open access

Quantification of Botrytis cinerea Growth in Arabidopsis thaliana

Description: Article describes how yield losses attributed to plant pathogens pose a serious threat to plant productivity and food security. Here, the authors describe two methods to quantify B. cinerea development in Arabidopsis thaliana genotypes through measurements of lesion development and quantification of fungal genomic DNA in infected tissues.
Date: August 20, 2023
Creator: Scholz, Patricia; Chapman, Kent Dean; Ischebeck, Till & Guzha, Athanas
Partner: UNT College of Science
open access

Identification and Characterization of an Arabidopsis thaliana Mutant with Tolerance to N-lauroylethanolamine

Description: N-Acylethanolamines (NAEs) are fatty acid derivatives in plants that negatively influence seedling growth. N-Lauroylethanolamine (NAE 12:0), one type of NAE, inhibits root length, increases radial swelling of root tips and reduces root hair numbers in a dose dependent manner in Arabidopis thaliana L. (ecotype Columbia). A forward genetics approach was employed by screening a population of T-DNA “activation-tagged” developed by the Salk Institute lines for NAE resistance to identify potential … more
Date: December 2015
Creator: Adhikari, Bikash
Partner: UNT Libraries
open access

LDIP cooperates with SEIPIN and LDAP to facilitate lipid droplet biogenesis in Arabidopsis

Description: Article showing that a recently identified protein termed LD-associated protein [LDAP]-interacting protein (LDIP) works together with both endoplasmic reticulum-localized SEIPIN and the LD-coat protein LDAP to facilitate LD formation in Arabidopsis thaliana. The data is discussed in the context of a new model for LD biogenesis in plant cells with evolutionary connections to LD biogenesis in other eukaryotes.
Date: June 9, 2021
Creator: Pyc, Michal; Gidda, Satinder K.; Seay, Damien C.; Esnay, Nicolas; Kretzschmar, Franziska K.; Cai, Yingqi et al.
Partner: UNT College of Science
open access

Lipophilic signals lead to organ-specific gene expression changes in Arabidopsis seedlings

Description: Article presents a study where whole transcriptome sequencing (RNA-seq) and differential expression analysis were used to identify early (1–3 hr) transcriptional changes induced by the exogenous treatment of NAE 18:2 and NAE 18:3 in cotyledons, roots, and seedlings.
Date: July 15, 2020
Creator: Cannon, Ashley E.; Yan, Chengshi; Burks, David J.; Rao, Xiaolan; Azad, Rajeev K. & Chapman, Kent Dean
Partner: UNT College of Science
open access

Metabolism and Action of Polyunsaturated N-acylethanolamines in Arabidopsis thaliana Seedlings

Description: The lipoxygenase (LOX) pathway plays an important role in the oxidative metabolism of polyunsaturated N-acylethanolamines (PU-NAEs). The LOX pathway functions in conjugation with hydrolysis by fatty acid amide hydrolase (FAAH) and to produce oxidized NAEs during seed germination and early seedling development. When Arabidopsis seedlings were grown in low micromolar concentrations of lauroylethanolamide (NAE 12:0), growth retardation and elevated endogenous PU-NAE levels were observed due to the… more
Date: August 2015
Creator: Keereetaweep, Jantana
Partner: UNT Libraries
open access

Time-resolved systems analysis of the induction of high photosynthetic capacity in Arabidopsis during acclimation to high light

Description: Article describes how induction of high photosynthetic capacity is a key acclimation response to high light for many herbaceous dicot plants. Here, a systems biology approach was utilized to characterize the induction of high photosynthetic capacity in strongly and weakly acclimating Arabidopsis thaliana accessions.
Date: October 17, 2023
Creator: Baker, Christopher R.; Cocuron, Jean-Christophe; Alonso, Ana Paula & Niyogi, Krishna K.
Partner: UNT College of Science
open access

Targeting the pattern-triggered immunity pathway to enhance resistance to Fusarium graminearum

Description: Article describes study which shows that resistance against fusarium graminearum, the primary casual agent of fusarium head blight (FHB), can be enhanced by flg22, a bacterial microbe-associated molecular pattern (MAMP).
Date: February 6, 2019
Creator: Sarowar, Sujon; Alam, Syeda T.; Makandar, Ragiba; Lee, Hyeonju; Trick, Harold N.; Dong, Yanhong et al.
Partner: UNT College of Science
open access

The impact of multifactorial stress combination on plant growth and survival

Description: Article studying seedlings of wild-type and different mutants of Arabidopsis thaliana plants subjected to a multifactorial stress combination of six different stresses, each applied at a low level, and their survival, physiological and molecular responses. Findings reveal that further polluting our environment could result in higher complexities of multifactorial stress combinations that in turn could drive a critical decline in plant growth and survival.
Date: January 26, 2021
Creator: Zandalinas, Sandra I.; Sengupta, Soham; Fritschi, Felix B.; Azad, Rajeev K.; Nechushtai, Rachel & Mittler, Ron
Partner: UNT College of Science
open access

Leaf Lipid Alterations in Response to Heat Stress of Arabidopsis thaliana

Description: Article measuring lipid levels with mass spectrometry to confirm the previously observed changes in Arabidopsis thaliana leaf lipids under three heat stress regimens. Additionally, in response to heat, increased oxidation of the fatty acyl chains of leaf galactolipids, sulfoquinovosyldiacylglycerols, and phosphatidylglycerols, and incorporation of oxidized acyl chains into acylated monogalactosyldiacylglycerols are shown. The authors also observed increased levels of digalactosylmonoacylglycero… more
Date: July 4, 2020
Creator: Shiva, Sunitha; Samarakoon, Thilani; Lowe, Kaleb A.; Roach, Charles; Vu, Hieu Sy; Colter, Madeline et al.
Partner: UNT College of Science
open access

Identification and characterization of a core set of ROS wave‐associated transcripts involved in the systemic acquired acclimation response of Arabidopsis to excess light

Description: Article reveal that the systemic transcriptomic response of plants to excess light stress is extensive in the context of systemic acquired acclimation and involves an early (2 min) and transient stage of transcript expression that includes thousands of genes.
Date: December 6, 2018
Creator: Zandalinas, Sara I.; Sengupta, Soham; Burks, David; Azad, Rajeev K. & Mittler, Ron
Partner: UNT College of Science
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