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Complex polymers of ADP-ribose occur in vitro and in vivo

Description: The work presented here included the development of a highly sensitive method to estimate the size and complexity of poly(ADP-ribose). This involved radiolabeling of the precursor pools, purification of polymers using a boronate resin, polymer fractionation according to size by molecular sieve chromatography and analysis of polymer complexity by enzymatic digestion to nucleotides which were quantified by strong anion exchange chromatography.
Date: May 1985
Creator: Alvarez-Gonzalez, Rafael
Partner: UNT Libraries

Identification of enzymatic processing of protein bound mono(ADP- ribose)

Description: Enzymatic activity has been identified in cultured cells which catalyzes the removal of intact mono(ADP-ribose) residues which are bound to protein at arginine. Other activities have been detected which catalyze the removal of ADP-ribose via the sequential removal of AMP and ribose-5-phosphate.
Date: December 1984
Creator: Smith, Kelly Payton
Partner: UNT Libraries

Identification of Endogenous Substrates for ADP-Ribosylation in Rat Liver

Description: Bacterial toxins have been shown to modify animal cell proteins in vivo with ADPR. Animal cells also contain endogenous enzymes that can modify proteins. Indirect evidence for the existence in vivo of rat liver proteins modified by ADPR on arginine residues has been reported previously. Presented here is direct evidence for the existence of ADP-ribosylarginine in rat liver proteins. Proteins were subjected to exhaustive protease digestion and ADP-ribosyl amino acids were isolated by boronate chromatography.
Date: May 1992
Creator: Loflin, Paul T. (Paul Tracey)
Partner: UNT Libraries

Poly(ADP-ribose) Synthesis as a Function of Growth and DNA Fragmentation

Description: This work examines the synthesis of poly(ADP-ribose) in normal and SV40-transformed monolayer cultures of 3T3 cells as a function of growth and DNA fragmentation. A review of the relevant literature is given in the introduction of this work. Poly(ADP-ribose) synthesis has been implicated in transcription, replication, repair, differentiation and regulation of cell growth. The results of this study suggest that poly(ADP-ribose) synthesis is involved in some aspect of cell-growth control and DNA repair.
Date: December 1981
Creator: Levi, Viktorya
Partner: UNT Libraries