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PRESERVATION OF H2 PRODUCTION ACTIVITY IN NANOPOROUS LATEX COATINGS OF RHODOPSEUDOMONAS PALUSTRIS CGA009 DURING DRY STORAGE AT AMBIENT TEMPERATURES

Description: To assess the applicability of latex cell coatings as an "off-the-shelf' biocatalyst, the effect of osmoprotectants, temperature, humidity and O{sub 2} on preservation of H{sub 2} production in Rhodopseudomonas palustris coatings was evaluated. Immediately following latex coating coalescence (24 h) and for up to 2 weeks of dry storage, rehydrated coatings containing different osmoprotectants displayed similar rates of H{sub 2} production. Beyond 2 weeks of storage, sorbitol- treated c… more
Date: August 27, 2012
Creator: Milliken, C.; Piskorska, M.; Soule, T.; Gosse, J.; Flickinger, M.; Smith, G. et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Research Frontiers in Bioinspired Energy: Molecular-Level Learning from Natural Systems: A Workshop

Description: An interactive, multidisciplinary, public workshop, organized by a group of experts in biochemistry, biophysics, chemical and biomolecular engineering, chemistry, microbial metabolism, and protein structure and function, was held on January 6-7, 2011 in Washington, DC. Fundamental insights into the biological energy capture, storage, and transformation processes provided by speakers was featured in this workshop�which included topics such as microbes living in extreme environments such as hydro… more
Date: March 28, 2012
Creator: Zolandz, Dorothy
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Genome Improvement at JGI-HAGSC

Description: Since the completion of the sequencing of the human genome, the JGI has rapidly expanded its scientific goals in several DOE mission-relevant areas. At the JGI-HAGSC, we have kept pace with this rapid expansion of projects with our focus on assessing, assembling, improving and finishing eukaryotic whole genome shotgun (WGS) projects for which the shotgun sequence is generated at the Production Genomic Facility (JGI-PGF). We follow this by combining the draft WGS with genomic resources generated… more
Date: March 3, 2012
Creator: Grimwood, Jane; Schmutz, Jeremy J. & Myers, Richard M.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Integrated Genome-Based Studies of Shewanella Ecophysiology

Description: Integration of bioinformatics and experimental techniques was applied to mapping and characterization of the key components (pathways, enzymes, transporters, regulators) of the core metabolic machinery in Shewanella oneidensis and related species with main focus was on metabolic and regulatory pathways involved in utilization of various carbon and energy sources. Among the main accomplishments reflected in ten joint publications with other participants of Shewanella Federation are: (i) A system… more
Date: December 17, 2012
Creator: Andrei L. Osterman, Ph.D.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department

Real-Time Imaging of Plant Cell Wall Structure at Nanometer Scale, with Respect to Cellulase Accessibility and Degradation Kinetics

Description: Presentation on real-time imaging of plant cell wall structure at nanometer scale. Objectives are to develop tools to measure biomass at the nanometer scale; elucidate the molecular bases of biomass deconstruction; and identify factors that affect the conversion efficiency of biomass-to-biofuels.
Date: May 1, 2012
Creator: Ding, S. Y.
Item Type: Refine your search to only Presentation
Partner: UNT Libraries Government Documents Department
open access

Deciphering the details of RNA aminoglycoside interactions: from atomistic models to biotechnological applications

Description: A detailed study was done of the neomycin-B RNA aptamer for determining its selectivity and binding ability to both neomycin– and kanamycin-class aminoglycosides. A novel method to increase drug concentrations in cells for more efficiently killing is described. To test the method, a bacterial model system was adopted and several small RNA molecules interacting with aminoglycosides were cloned downstream of T7 RNA polymerase promoter in an expression vector. Then, the growth analysis of E. coli … more
Date: July 23, 2012
Creator: Ilgu, Muslum
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries Government Documents Department
open access

Salt Dependence of the Radius of Gyration and Flexibility of Single-stranded DNA in Solution probed by Small-angle X-ray Scattering

Description: Short single-stranded nucleic acids are ubiquitous in biological processes and understanding their physical properties provides insights to nucleic acid folding and dynamics. We used small angle x-ray scattering to study 8-100 residue homopolymeric single-stranded DNAs in solution, without external forces or labeling probes. Poly-T's structural ensemble changes with increasing ionic strength in a manner consistent with a polyelectrolyte persistence length theory that accounts for molecular flex… more
Date: July 6, 2012
Creator: Sim, Adelene Y.L.; Lipfert, Jan; Herschlag, Daniel & Doniach, Sebastian
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

National Bioenergy Center Biochemical Platform Integration Project Quarterly Update, Winter 2011-2012

Description: Quarterly newsletter describing the activities of the National Bioenergy Center's Biochemical Platform Integration Project including ongoing research and goals. Issue topics: 34th Symposium on Biotechnology for Fuels and Chemicals; feasibility of NIR spectroscopy-based rapid feedstock reactive screening; demonstrating integrated pilot-scale biomass conversion. The Biochemical Process Integration Task focuses on integrating the processing steps in enzyme-based lignocellulose conversion technolo… more
Date: April 2012
Creator: National Renewable Energy Laboratory (U.S.)
Item Type: Refine your search to only Journal/Magazine/Newsletter
Partner: UNT Libraries Government Documents Department
open access

Integration of Carbon, Nitrogen, and Oxygen Metabolism in Escherichia coli--Final Report

Description: A key challenge for living systems is balancing utilization of multiple elemental nutrients, such as carbon, nitrogen, and oxygen, whose availability is subject to environmental fluctuations. As growth can be limited by the scarcity of any one nutrient, the rate at which each nutrient is assimilated must be sensitive not only to its own availability, but also to that of other nutrients. Remarkably, across diverse nutrient conditions, E. coli grows nearly optimally, balancing effectively the con… more
Date: October 22, 2012
Creator: Rabinowitz, Joshua D; Wingreen, Ned s; Rabitz, Herschel A & Xu, Yifan
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

IDENTIFICATION, PRODUCTION AND CHARACTERIZATION OF NOVEL LIGNASE PROTEINS FROM TERMITES FOR DEPOLYMERIZATION OF LIGNOCELLULOSE

Description: Wood is a potential source for biofuels such as ethanol if it can be digested into sugars and fermented by yeast. Biomass derived from wood is a challenging substrate for ethanol production since it is made of lignin and cellulose which cannot be broken down easily into fermentable sugars. Some insects, and termites in particular, are specialized at using enzymes in their guts to digest wood into sugars. If termite gut enzymes could be made abundantly by a recombinant protein expression vector … more
Date: December 6, 2012
Creator: Slack, Jeffrey M.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Isolation, Preliminary Characterization and Preliminary Assessment of Scale-Up Potential of Photosynthetic Microalgae for the Production of Both Biofuels and Bio-Active Molecules in the U.S. and Canada: Cooperative Research and Development Final Report, CRADA Number CRD-10-372

Description: Combustion flue gases are a major contributor to carbon dioxide emissions into the Earth's atmosphere, a factor that has been linked to the possible global climate change. It is, therefore, critical to begin thinking seriously about ways to reduce this influx into the atmosphere. Using carbon dioxide from fossil fuel combustion as a feedstock for the growth, photosynthetic microorganisms can provide a large sink for carbon assimilation as well as a feedstock for the production of significant le… more
Date: September 1, 2012
Creator: Pienkos, P.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Final Progress Report

Description: The BIOMOL grant was for 'Local System Support for PDB Biological Unit Search and Display' to augment Rasmol's [Bernstein 2000] [Sayle, Milner-White 1995] existing macromolecular display functions with new capabilities by taking advantage of recent increases in local computing power in order to move functionality that is now scattered among various local and remote systems into one local package. Work included new algorithms for molecular surface display, an extended format for Protein Data Ban… more
Date: February 6, 2012
Creator: Bernstein, Herbert J.
Item Type: Refine your search to only Text
Partner: UNT Libraries Government Documents Department
open access

Glycosylation Helps Cellulase Enzymes Bind to Plant Cell Walls (Fact Sheet)

Description: Computer simulations suggest a new strategy to design enhanced enzymes for biofuels production. Large-scale computer simulations predict that the addition of glycosylation on carbohydrate-binding modules can dramatically improve the binding affinity of these protein domains over amino acid mutations alone. These simulations suggest that glycosylation can be used as a protein engineering tool to enhance the activity of cellulase enzymes, which are a key component in the conversion of cellulose t… more
Date: June 1, 2012
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Coherent Anti-Stokes Raman Scattering Spectroscopy of Single Molecules in Solution

Description: During this funding period, we have developed two breakthrough techniques. The first is stimulated Raman scattering microscopy, providing label-free chemical contrast for chemical and biomedical imaging based on vibrational spectroscopy. Spontaneous Raman microscopy provides specific vibrational signatures of chemical bonds, but is often hindered by low sensitivity. We developed a three-dimensional multiphoton vibrational imaging technique based on stimulated Raman scattering (SRS). The sensiti… more
Date: January 18, 2012
Creator: Sunney Xie, Wei Min, Chris Freudiger, Sijia Lu
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Integrated genome-based studies of Shewanella ecophysiology

Description: This project was a component of the Shewanella Federation and, as such, contributed to the overall goal of applying the genomic tools to better understand eco-physiology and speciation of respiratory-versatile members of Shewanella genus. Our role at Boston University was to perform bioreactor and high throughput gene expression microarrays, and combine dynamic flux balance modeling with experimentally obtained transcriptional and gene expression datasets from different growth conditions. In th… more
Date: February 14, 2012
Creator: Daniel, Segre & Qasim, Beg
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Maltose Biochemistry and Transport in Plant Leaves

Description: Starch is a desirable plant product for both food and biofuel. Leaf starch is ideal for use in biofuels because it does not compete with grain starch, which is used for food. Starch is accumulated in plant leaves during the day and broken down at night. If we can manipulate leaf starch breakdown it may be possible to design a plant that provides both grain starch for food and leaf starch for biofuel. The pathway of leaf starch breakdown was not known when this work started. Preliminary evidence… more
Date: May 3, 2012
Creator: Sharkey, Thomas D
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Interim Report on Multiple Sequence Alignments and TaqMan Signature Mapping to Phylogenetic Trees

Description: The goal of this project is to develop forensic genotyping assays for select agent viruses, addressing a significant capability gap for the viral bioforensics and law enforcement community. We used a multipronged approach combining bioinformatics analysis, PCR-enriched samples, microarrays and TaqMan assays to develop high resolution and cost effective genotyping methods for strain level forensic discrimination of viruses. We have leveraged substantial experience and efficiency gained through y… more
Date: March 27, 2012
Creator: Gardner, S & Jaing, C
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Laboratory Directed Research and Development Annual Report for 2011

Description: This report documents progress made on all LDRD-funded projects during fiscal year 2011. The following topics are discussed: (1) Advanced sensors and instrumentation; (2) Biological Sciences; (3) Chemistry; (4) Earth and space sciences; (5) Energy supply and use; (6) Engineering and manufacturing processes; (7) Materials science and technology; (8) Mathematics and computing sciences; (9) Nuclear science and engineering; and (10) Physics.
Date: April 9, 2012
Creator: Hughes, Pamela J.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

The Energy Budget of Steady State Photosynthesis

Description: Progress is reported in addressing these questions: Why do hcef mutants have increased CEF1? Is increased CEF1 caused by elevated expression or altered regulation of CEF1 components? Which metabolic pools can be regulators of CEF1? Do metabolites influence CEF1 directly or indirectly? Which CEF1 pathways are activated in high CEF1 mutants? Is PQR a proton pump? Is elevated CEF1 activated by state transitions?
Date: November 27, 2012
Creator: Kramer, Dr. David M.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Summary of the International Conference on Arabidopsis Research 2011, June 22-25, 2011

Description: This project provided participant support for the gathering of plant biologists at the International Conferences on Arabidopsis Research (ICAR) in 2011. Arabidopsis thaliana, the reference flowering plant, has been intensely studied over the last 20 years and has proven to be an ideal model for studying nearly all aspects of plant biology. The success of this research field has been greatly facilitated by the openness and collegiality of the community fostered through multiple international for… more
Date: July 15, 2012
Creator: Meyers, Blake C
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Reconstruction of a Bacterial Genome from DNA Cassettes

Description: This basic research program comprised two major areas: (1) acquisition and analysis of marine microbial metagenomic data and development of genomic analysis tools for broad, external community use; (2) development of a minimal bacterial genome. Our Marine Metagenomic Diversity effort generated and analyzed shotgun sequencing data from microbial communities sampled from over 250 sites around the world. About 40% of the 26 Gbp of sequence data has been made publicly available to date with a compl… more
Date: 2012~
Creator: Dupont, Christopher; Glass, John; Sheahan, Laura; Yooseph, Shibu; Allen, Lisa Zeigler; Thiagarajan, Mathangi et al.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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