14 Matching Results

Search Results

Advanced search parameters have been applied.

Large scale solubilization of coal and bioconversion to utilizable energy. Quarterly report, October 1--December 31, 1996

Description: In order to develop a system for a large scale coal solubilization and its bioconversion to utilizable fuel, the author plans to clone the genes encoding Neurospora protein that facilitate depolymerization of coal. He also plans to use desulfurizing bacteria to remove the sulfur in situ and use other microorganisms to convert biosolubilized coal into utilizable energy following an approach utilizing several microorganisms. In addition the product of coal solubilized by fungus will be characterized to determine their chemical nature and the mechanism of reaction catalyzed by fungal product during in vivo and in vitro solubilization by the fungus or purified fungal protein.
Date: December 22, 1996
Creator: Mishra, N.C.
Partner: UNT Libraries Government Documents Department

Large scale solubilization of coal and bioconversion to utilizable energy. Eleventh quarterly technical progress report, April 1, 1996--June 30, 1996

Description: Neurospora has the capability to solubilize coal and the protein fraction accounting for this ability has been isolated. During this period the cola solubilizing activity (CSA) was fractionated and partially sequenced. The activity has been determined to be a tyrosinase and/or a phenol oxidase. The amino acid sequence of the protein was used to prepare oligonucleotides to identify the clone carrying Neurospora CSA. It is intended to clone the Neurospora gene into yeast, since yeast cannot solubilize coal, to further characterize the CSA.
Date: October 1, 1996
Creator: Mishra, N.C.
Partner: UNT Libraries Government Documents Department

Large scale solubilization of coal and bioconversion to utilizable energy. Eighth quarterly technical progress report, July 1, 1995--September 30, 1995

Description: In order to develop a system for a large scale coal solubilization and its bioconversion to utilizable fuel, we plan to clone the genes encoding Neurospora protein that facilitate depolymerization of coal. We also plan to use desulfurizing bacteria to remove the sulfur in situ and use other microorganisms to convert biosolubilized coal into utilizable energy following an approach utilizing several microorganisms. In addition the product of coal solubilized by fungus will be characterized to determine their chemical nature and the mechanism of reaction catalyzed by fungal product during in vivo and in vitro solubilization by the fungus or purified fungal protein.
Date: February 1, 1996
Creator: Mishra, N.C.
Partner: UNT Libraries Government Documents Department

Final report of DOE Grant DE-FG02-92ER61384, March 1, 1993--February 28, 1995 with no-cost extension to February 29, 1996

Description: This report focuses mainly on the effects that various plants may incur from exposure to elevated levels of carbon dioxide. Some experiments also examined the responses of insects to plants growing under elevated levels of carbon dioxide. Projected changes in carbon dioxide are presumed to be accompanied by altered climates, particularly elevated temperatures and perhaps altered moisture regimes.
Date: June 1, 1997
Creator: Lincoln, D.E.
Partner: UNT Libraries Government Documents Department

Exploration of new perspectives and limitations in Agrobacterium mediated gene transfer technology. Progress report, [June 1, 1992-- May 31, 1994]

Description: This report describes progress aimed at constructing gene-transfer technology for Nicotiana plumbaginifolia. Most actual effort as described herein has so far been directed at exploring new perspectives and limitations in Agrobacterium mediated gene transfer. Accomplishments are described using a core homologous gene targeting vector.
Date: December 31, 1994
Creator: Marton, L.
Partner: UNT Libraries Government Documents Department

Large scale solubilization of coal and bioconversion to utilizable energy. Seventh quarterly technical progress report, April 1, 1995--June 30, 1995

Description: In order to develop a system for a large scale coal solubilization and its bioconversion to utilizable fuel, we plan to clone the genes encoding Neurospora protein that facilitate depolymerization of coal. We also plan to use desulfurizing bacteria to remove the sulfur in situ and use other microorganisms to convert biosolubilized coal into utilizable energy following an approach utilizing several microorganisms. In addition the product of coal solubilized by fungus will be characterized to determine their chemical nature and the mechanism of reaction catalyzed by fungal product during in vivo and in vitro solubilization by the fungus or purified fungal protein.
Date: December 1, 1995
Creator: Mishra, N.C.
Partner: UNT Libraries Government Documents Department

Exploration of new perspectives and limitations in Agrobacterium-mediated gene transfer technology. Final report, June 1, 1992--May 31, 1995

Description: Genetic manipulation of plants often involves the introduction of homologous or partly homologous genes. Ectropic introduction of homologous sequences into plant genomes may trigger epigenetic changes, making expression of the genes unpredictable. The main project objective was to examine the feasibility of using Agrobacterium-mediated gene transfer for homologous gene targeting in plants.
Date: February 1, 1996
Creator: Marton, L.
Partner: UNT Libraries Government Documents Department

[Plant responses to elevated atmospheric carbon dioxide and transmission to other trophic levels]. Progress report, May 1991, DOE Grant DE-FG09-84ER60255

Description: Experiments were performed to determine the effects of carbon dioxide on plants and on the insects feeding on these plants. Current progress is reported for the following experiments: Response of a Specialist-Feeding Insect Herbivore to Carbon Dioxide Induced Changes in Its Hostplant; Growth and Reproduction of Grasshoppers Feeding on a C{sub 4} Grass Under Elevated Carbon Dioxide; Elevated Carbon Dioxide and Temperature Effects on Growth and Defense of Big Sagebrush; Sagebrush and Grasshopper Responses to Atmospheric Carbon Dioxide Concentration; Biomass Allocation Patterns of Defoliated Sagebrush Grown Under Two Levels of Carbon Dioxide; and Sagebrush Carbon Allocation Patterns and Grasshopper Nutrition: The Influence of Carbon Dioxide Enrichment and Soil Mineral Limitation.
Date: May 1, 1991
Creator: Lincoln, D.E.
Partner: UNT Libraries Government Documents Department

[Plant responses to elevated atmospheric carbon dioxide and transmission to other trophic levels]. Final report

Description: This program investigated how host plant responses to elevated atmospheric carbon dioxide may be transmitted to other trophic levels, especially leaf eating insects, and alter consumption of leaves and impare their function. Study results included the following findings: increased carbon dioxide to plants alters feeding by insect herbivores; leaves produced under higher carbon conditions contain proportionally less nitrogen; insect herbivores may have decreased reproduction under elevated carbon dioxide.
Date: October 1, 1995
Creator: Lincoln, D.E.
Partner: UNT Libraries Government Documents Department

Large scale solubilization of coal and bioconversion to utilizable energy. Fifth quarterly technical report, January 1, 1995--March 31, 1995

Description: In order to develop a system for a large scale coal solubilization and its bioconversion to utilizable fuel, we plan to clone the genes encoding Neurospora protein that facilitate depolymerization of coal. We also plan to use desulfurizing bacteria to remove the sulfur in situ and use other microorganisms to convert biosolubilized coal into utilizable energy following an approach utilizing several microorganisms. In addition the product of coal solubilized by fungus will be characterized to determine their chemical nature and the mechanism of reaction catalyzed by fungal product during in vivo and in vitro solubilization by the fungus or purified fungal protein.
Date: December 1, 1995
Creator: Mishra, N.C.
Partner: UNT Libraries Government Documents Department

Characterization of the mammalian DNA polymerase gene(s) and enzyme(s). Annual progress report

Description: Consistent with the long term goal of our research to understand the nature of the key enzymes in eukaryotic DNA replication we have characterized the properties of the wild type DNA polymerases of the {alpha}-family and their mutants. We have also provided evidence for the role of aphidicolin in the elongation process of the in vivo DNA replication in eukaryotic cells. We also developed a technology for planned prep from a large numbers of clones for direct screening by size or restriction digestion in order to facilitate our goals to clone the DNA polymerase gene.
Date: January 1, 1994
Creator: Mishra, N.C.
Partner: UNT Libraries Government Documents Department

Large scale solubilization of coal and bioconversion to utilizable energy. Quarterly technical progress report, January-March 1994

Description: In order to develop a system for large scale coal solubilization and its bioconversion to utilizable fuel, the authors plan to clone the genes encoding Neurospora protein that facilitate depolymerization of coal. They also plan to use desulfurizing bacteria to remove the sulfur in situ and use other microorganisms to convert biosolubilized coal into utilizable energy following an approach utilizing several microorganisms (Faison). In addition the product of coal solubilized by fungus will be characterized to determine their chemical nature and the mechanism of reaction catalyzed by fungal product during in vivo and in vitro solubilization by the fungus or purified fungal protein.
Date: June 1, 1994
Creator: Mishra, N. C.
Partner: UNT Libraries Government Documents Department

[Incremental funding request]

Description: Two separate experiments described here involves using early (2nd) instar larvae on plants with four levels of CO{sub 2} with no nutrient limitation, and using penultimate instar (5th) larvae fed plants grown under four CO{sub 2} and two nutrient levels in two feeding trials from plants from two different seasons, spring and fall. For each experiment the CO{sub 2} plant growth conditions were the same: pine seedlings were grown in open-topped chambers under three levels of CO{sub 2}: (1) ambient (2) 150 {mu}L/L CO{sub 2} above ambient and (3) 300 {mu}L/L above ambient. A fourth treatment, non-chambered ambient (NC) was used to assess chamber effects. Nutrient treatments were either low nitrogen or nutrient unlimited. Performance of larvae was determined by calculating the relative growth and consumption rates, as well as the digestibility of the larvae. Leaf samples were taken to determine leaf nitrogen, starch, and water contents.
Date: May 1, 1994
Creator: Lincoln, D. E.
Partner: UNT Libraries Government Documents Department

Large scale solubilization of coal and bioconversion to utilizable energy. Quarterly technical progress report, September--December 1993

Description: In order to develop a system for a large scale coal solubilization and its bioconversion to utilizable fuel, we plan to clone the genes encoding Neurospora protein that facilitate depolymerization of coal. We also plan to use desulfurizing bacteria to remove the sulfur in situ and use other microorganisms to convert biosolubilized coal into utilizable energy following an approach utilizing several microorganisms (Faison, 1991). In addition the product of coal solubilized by fungus will be characterized to determine their chemical nature and the mechanism of reaction catalyzed by fungal product during in vivo and in vitro solubilization by the fungus or purified fungal protein. The main objectives are: (1) Cloning of Neurospora gene for coal depolymerization protein controlling solubilization in different host cells, utilizing Neurospora plasmid and other vector(s); (2) (a) Development of a large scale electrophoretic separation of coal-drived products obtained after microbial solubilization; (b) Identification of the coal derived products obtained after biosolubilization by Neurospora cultures or obtained after Neurospora enzyme catalyzed reaction in in vitro by the wildtype and mutant enzymes; and (3) Bioconversion of coal-derived products into utilizable fuel.
Date: December 31, 1993
Creator: Mishra, N. C.
Partner: UNT Libraries Government Documents Department