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Evidence for the Interaction of GTP with Rat Liver Glyoxalase II

Description: Glyoxalase 11, the second enzyme of the glyoxalase system, hydrolyzes S-D-lactoylglutathione (SLG) to regenerate glutathione (GSH) and liberate free D-lactate. It was found that GTP binds with Gil from rat liver and inhibits Gil activity. Preincubation experiments showed that the binding is relatively tight, since more than 15 minutes are required to release GTP from the complex following dilution. Inhibition kinetics studies indicate that GTP is a "partially competitive inhibitor"; Thus, it would appear that the binding sites for substrate (SLG) and inhibitor (GTP) are different, but spatially close. Glyoxalase 11 binds to a GTP affinity medium, and with polyacrylamide gel electrophoresis, Gil has a higher relative mobility when GTP is present (ATP has no effect). The functional consequences of GTP binding with a specific site on Gil are still unclear. It is speculated that Gil may interact with tubulin by serving as a dissociable GTP carrier, delivering GTP to the tubulinGTP binding site, and thus facilitating tubulin polymerization.
Date: December 1991
Creator: Yuan, Win-Jae
Partner: UNT Libraries

Toward a Critique of the Message Construct in Communication

Description: The idea of an inherent structure of meaning in human communication is identified as the message construct. Traces of the construct in textbook models of the basic communication process and in popular magazine images of communication are examined. The argument is raised that objectifying the message has resulted in a paradigm which focuses disproportionately on explicit, representational, and instrumental aspects of communication. An alternative conception is proposed which would take into account implicit, constitutive, and generative aspects.
Date: August 1991
Creator: Wilson, John K. (John Kenneth)
Partner: UNT Libraries

The Writers and Writing of Computer User Documentation: A Social Perspective

Description: This thesis studies the writing of computer user documentation from a social perspective by examining the process of creating computer documentation and the role of documentation writers in the work place. This study consisted of in-depth interviews and observations of four writers of computer user documentation.
Date: August 1991
Creator: Webb, Sheree C.
Partner: UNT Libraries

Factor Analysis of Health Concerns in the Chronic Back Pain Patient-MMPI2

Description: The purpose of this study was to analyze the factor structure of items pertaining to health on the Minnesota Multiphasic Personality Inventory (MMPI2) for chronic back pain patients in comparison to a control group. The results may be used as groundwork for developing an MMPI2 subscale to describe this population. The groups differed in the sequence of the resulting factors and the percentage of variance accounted for by each factor. The factors extracted when evaluating the control group were titled in order: Poor Physical Health, Digestive Difficulties, Equilibrium, Depression/Malaise, and Multiple Somatic Complaints. Resulting factors for the pain group were: Depression/Malaise, Digestive Difficulties, Multiple Somatic Complaints, Headaches/Dizziness, and Neurological Reaction/Poor Physical Health.
Date: August 1991
Creator: McGee-Hall, Joanne M. (Joanne Moore)
Partner: UNT Libraries

Sleep Patterns and Chronic Pain

Description: Sleep, emotions and pain are intimately connected, physiologically, by their location and utilization of the same brain centers and neurotransmitters. Sleep disturbances have been clinically observed in chronic pain populations; yet, no treatment program has formally addressed this aspect of patient care. It is hypothesized that a pain population (PN) will differ significantly from a non-injured workforce (WF) when reviewing quantitative and qualitative sleep data. This study strongly supports that sleep disturbances and socioeconomic decrements exist in chronic pain patients. Forty-seven variables were surveyed and 13 were found to show significant differences between the groups and seven were found to discriminate between the PN and WF groups at less than the .0001 level. A discriminant analysis was performed to determine the smallest model which could efficiently classify cases, according to successive root variables. The major discriminators are pain levels, medication, amount of sleep obtained and number of awakenings.
Date: August 1991
Creator: Kellen, Rebecca Margaret
Partner: UNT Libraries

Synthesis and Kinetic Mechanism Study of Phosphonopeptide as a Dead-End Inhibitor of cAMP-Dependent Protein Kinase

Description: DL-2-Amino-4-phosphonobutyric acid, an isostere of phosphoserine, was incorporated into the heptapeptide sequence, Leu-Arg-Arg-Ala-(DL-2-amino-4-phosphonobutyric acid)-Leu-Gly, for kinetic mechanistic studies of the cAMP-dependent protein kinase. To block the phosphono hydroxyl groups, methyl, ethyl and 4nitrobenzyl esters were studied as possible protecting groups. The phosphono diethyl ester of the N-Fmoc-protected amino acid was utilized in the synthesis of the heptapeptide. Two configurational forms of the protected peptide were obtained and were separated by C18-reverse phase HPLC. Characterization of the two isomeric forms was accomplished by 3 1P NMR, 1H NMR, 13C% NMR and amino acid analysis. The protecting groups of the isomeric phsophonopeptides were removed by HBr/AcOH and purified by cation exchange HPLC. Both phosphonopeptides were found to be inhibitors of the cAMP-dependent protein kinase, having Ki values of 0.6 mM (peptide A) and 1.9 mM (peptide B).
Date: December 1991
Creator: Yang, Chunhua
Partner: UNT Libraries

Personality Profiles of Hospitality Students: A Comparison of These Traits to Those Preferred by the Hospitality Industry

Description: One problem facing the hospitality industry today is turnover. Management turnover rates of 50 and 75 percent continue to plaque all segments of the industry. Personality type theory holds that people are happier in environments that are compatible with their personalities. This study examines 229 undergraduate students enrolled in hospitality education at the University of North Texas. The Myers Briggs Type Indicator was administered to these students to determine their predominant personality types, and to compare these types to those desired by hospitality industry professionals for success within the industry. Variables such as gender, work experience, and classification were also examined in comparison to student personality types.
Date: December 1991
Creator: Martin, Lynda (Lynda Jean)
Partner: UNT Libraries

D. H. Lawrence: Misogyny as Ideology in His Later Works of Fiction and Nonfiction

Description: Critics continue to debate Lawrence's attitude toward women: Some say Lawrence is a misogynist, some say he is an egalitarian, and others say he is ambivalent toward women. If Lawrence's works are divided into two chronological periods, before and after 1918, these differences of opinions begin to dissolve. Lawrence is fair in his treatment of women in the earlier works; however, in his later works Lawrence restricts women to what he calls the sensual realm, the realm of feelings and emotions. In addition, Lawrence denounces all women who assert individuality and self-responsibility. In the later works, Lawrence's ideology restricts the role of women and presents male supremacy as the natural and necessary order for human existence.
Date: August 1991
Creator: Hester, Vicki M. (Vicki Martin)
Partner: UNT Libraries

Pre-Steady State Kinetics of the NAD-Malic Enzyme from Ascaris suum in the Direction of Oxidative Decarboxylation of L-Malate

Description: Stopped-flow experiments in which the NAD-malic enzyme was preincubated with different reactants at near saturating substrate concentrations suggest a slow isomerization of the E:NAD:Mg complex. The lag is eliminated by preincubation with Mg˙² and malate suggesting that the formation of E:Mg:Malate either bypasses or speeds up the slow isomerization step. Circular dichroic spectral studies of the secondary structural changes of the native enzyme in the presence and absence of substrates supports the existence of conformational changes with NAD˙ and malate. Thus, a slow conformational change of the E:NAD:Mg complex is likely one of the rate-limiting steps in the pre-steady state.
Date: December 1991
Creator: Rajapaksa, Ranjani, 1949-
Partner: UNT Libraries

Linking Parent Relationships with Intimacy in a Selected Group of Young Adult University Students

Description: Literature suggests positive relationships with caregivers during childhood facilitate intimacy in young adulthood. The three hypotheses in this inquiry related to the students' relationship between parental intimacy and friend intimacy, gender differences in intimacy, and the perceived acceptance of parents. Subjects were 322 male and female university students, aged 17 through 25 years. Most were single, white, and middle class. During class the Children's Report of Parental Behavior, the Miller Social Intimacy Scale, and a demographic sheet were administered. ANOVA revealed that relationships between parental intimacy and friend intimacy were not significant. Females reported greater intimacy with friends than males. For both sexes, correlations between recalled parental intimacy and acceptance were higher for mothers than fathers.
Date: August 1991
Creator: Roland, Sandra Dodson
Partner: UNT Libraries

A Comprehensive Neuropsychological Screening Device for Adults: Reliability of Parallel Forms

Description: The purpose of the present study was to evaluate the reliability of parallel-forms of the Comprehensive Neuropsychological Screening Device (CNS). Forty-five subjects ranging in age from 16 to 69 were administered Form A and Form B of the CNS at two week intervals. Results indicated that the CNS has adequate test-retest reliability. The results suggest the applicability of using the CNS as a screening device for brain dysfunction.
Date: December 1991
Creator: Ganci, Maria
Partner: UNT Libraries