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in creator/contributor: "Stapleton, Mark"  
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RNA editing in Drosophila melanogaster: new targets and functionalconsequences

Description: Adenosine deaminases that act on RNA (ADARs) catalyze the site-specific conversion of adenosine to inosine in primary mRNA transcripts. These re-coding events affect coding potential, splice-sites, and stability of mature mRNAs. ADAR is an essential gene and studies in mouse, C. elegans, and Drosophila suggest its primary function is to modify adult behavior by altering signaling components in the nervous system. By comparing the sequence of isogenic cDNAs to genomic DNA, we have identified and… more
Date: September 5, 2006
Creator: Stapleton, Mark; Carlson, Joseph W. & Celniker, Susan E.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

High-Throughput Plasmid cDNA Library Screening

Description: Libraries of cDNA clones are valuable resources foranalysing the expression, structure, and regulation of genes, as well asfor studying protein functions and interactions. Full-length cDNA clonesprovide information about intron and exon structures, splice junctionsand 5'- and 3'-untranslated regions (UTRs). Open reading frames (ORFs)derived from cDNA clones can be used to generate constructs allowingexpression of native proteins and N- or C-terminally tagged proteins.Thus, obtaining full-length… more
Date: May 24, 2006
Creator: Wan, Kenneth H.; Yu, Charles; George, Reed A.; Carlson, JosephW.; Hoskins, Roger A.; Svirskas, Robert et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Rapid and Efficient cDNA Library Screening by Self-Ligation ofInverse PCR Products (SLIP)

Description: The production of comprehensive cDNA clone collections is an important goal of the human and model organism genome projects. cDNA sequences are used to determine the structures of transcripts, including splice junctions, polyadenylation sites, and 5' and 3' untranslated regions (UTRs). cDNA collections are also valuable resources for functional studies of genes and proteins. Expressed Sequence Tag (EST)sequencing is the method of choice for recovering cDNAs representing a majority of the transc… more
Date: April 22, 2005
Creator: Hoskins, Roger A.; Stapleton, Mark; George, Reed A.; Yu, Charles; Wan, Kenneth H.; Carlson, Joseph W. et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Developmental roles of 21 Drosophila transcription factors are determined by quantitative differences in binding to an overlapping set of thousands of genomic regions

Description: BACKGROUND: We previously established that six sequence-specific transcription factors that initiate anterior/posterior patterning in Drosophila bind to overlapping sets of thousands of genomic regions in blastoderm embryos. While regions bound at high levels include known and probable functional targets, more poorly bound regions are preferentially associated with housekeeping genes and/or genes not transcribed in the blastoderm, and are frequently found in protein coding sequences or in less … more
Date: May 15, 2009
Creator: MacArthur, Stewart; Li, Xiao-Yong; Li, Jingyi; Brown, James B.; Chu, Hou Cheng; Zeng, Lucy et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Transcription Factors Bind Thousands of Active and InactiveRegions in the Drosophila Blastoderm

Description: Identifying the genomic regions bound by sequence-specific regulatory factors is central both to deciphering the complex DNA cis-regulatory code that controls transcription in metazoans and to determining the range of genes that shape animal morphogenesis. Here, we use whole-genome tiling arrays to map sequences bound in Drosophila melanogaster embryos by the six maternal and gap transcription factors that initiate anterior-posterior patterning. We find that these sequence-specific DNA binding … more
Date: January 10, 2008
Creator: Li, Xiao-Yong; MacArthur, Stewart; Bourgon, Richard; Nix, David; Pollard, Daniel A.; Iyer, Venky N. et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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