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Results of Toxicity Studies Conducted on Ceriodaphnia dubia and Daphnia ambigua in Support of an Alternate Species Demonstration, January - June 2000

Description: In 1997, the Savannah River Site completed toxicity testing studies on an alternate species, Daphnia ambigua, that we have proposed for use as a toxicity testing organism at SRS. We demonstrated that this species could be cultured in the laboratory and that it was at least as sensitive as Ceriodaphnia dubia to a broad range of toxicants (Specht and Harmon, 1997; Harmon, 1998; Harmon and Specht, 1998; Harmon, Specht and Chandler, 1999). However, it performed better that C. dubia in very soft water, which is representative of many SRS effluents and receiving waters. In January 2000, representatives from SRS met with representatives from U.S. EPA Region 4 and SCDHEC to discuss data needs related to EPA's consideration of SRS's request to use the alternate species (D. ambigua) for routine toxicity testing at SRS. SRS contends that the very low water hardness of some of its effluents is responsible for toxicity failures because the species recommended by the EPA (C. dubia) does not reproduce well in waters that have very low hardness.
Date: January 14, 2003
Creator: Specht, W.L.
Partner: UNT Libraries Government Documents Department

Results of Toxicity Identification Evaluations (TIE`S) conducted on the A-01 outfall and its contributory waste streams, July 1996--February 1997

Description: Toxicity tests were conducted at nine locations during the summer of 1996. The results indicated that A-01B, A-01C, A-03, A-04, A-05 and A-01 were toxic to the test species, Ceriodaphnia dubia, while A-01A, A-06, and WE-01 were not toxic. Beginning in August 1996, Toxicity Identification Evaluations (TIE`s) were initiated on all toxic outfalls in order to identify the toxicants responsible for the observed toxicity. A complete TIE was performed on A-01 because it is the regulatory compliance point for all of the combined waste streams that were tested. Only the portions of a TIE that are related to metal and chlorine toxicity were performed on the remaining locations because existing data indicated that metals and chlorine were present in potentially toxic quantities at these locations, and there was no evidence that other toxicants would be expected to be present in toxic amounts. The results of the TIE`s indicate that metals are responsible for most of the toxicity at all of the outfalls that were toxic and that chlorine contributed to the toxicity at two of the outfalls. Specifically, the toxicity at A-01B, A-01C, and A-01 was due to copper; the toxicity at A-03 was due to primarily to copper, although zinc also contributed to the toxicity; the toxicity at A-04 was due primarily to copper, with residual chlorine and zinc contributing to the toxicity; and the toxicity at A-05 was due primarily to copper, with residual chlorine contributing to the toxicity. A-03 was the most toxic outfall, with 100% mortality occurring at concentrations as low as 12.5% effluent. A-03 was found to have concentrations of copper, lead, and zinc that exceeded EPA water quality criteria by approximately two orders of magnitude. The metal concentrations at A-01 and WE-01, which is located approximately 0.5 miles downstream from A-01 were similar. However, ...
Date: March 1, 1997
Creator: Specht, W.L.
Partner: UNT Libraries Government Documents Department

L-Lake macroinvertebrate community

Description: To characterize the present benthic macroinvertebrate community of L-Lake, Regions 5 and 7 of the reservoir were sampled in September 1995 at the same locations sampled in 1988 and 1989 during the L-Lake monitoring program. The macroinvertebrate community of 1995 is compared to that of 1988 and 1989. The species composition of L-Lake`s macroinvertebrate community has changed considerably since 1988-1989, due primarily to maturation of the reservoir ecosystem. L-Lake contains a reasonably diverse macroinvertebrate community that is capable of supporting higher trophic levels, including a diverse assemblage of fish species. The L-Lake macroinvertebrate community is similar to those of many other southeastern reservoirs, and there is no indication that the macroinvertebrate community is perturbed by chemical or physical stressors.
Date: June 1, 1996
Creator: Specht, W.L.
Partner: UNT Libraries Government Documents Department

Instream Biological Assessment of NPDES Point Source Discharges at the Savannah River Site, 2000

Description: The Savannah River Site (SRS) currently has 31 NPDES outfalls that have been permitted by the South Carolina Department of Health and Environmental Control (SCDHEC) to discharge to SRS streams and the Savannah River. In order to determine the cumulative impacts of these discharges to the receiving streams, a study plan was developed to perform in-stream assessments of the fish assemblages, macroinvertebrate assemblages, and habitats of the receiving streams. These studies were designed to detect biological impacts due to point source discharges. Sampling was initially conducted between November 1997 and July 1998 and was repeated in the summer and fall of 2000. A total of 18 locations were sampled (Table 1, Figure 1). Sampling locations for fish and macroinvertebrates were generally the same. However, different locations were sampled for fish (Road A-2) and macroinvertebrates (Road C) in the lower portion of Upper Three Runs, to avoid interference with ongoing fisheries studies at Road C. Also, fish were sampled in Fourmile Branch at Road 4 rather than at Road F because the stream at Road F was too narrow and shallow to support many fish. Sampling locations and parameters are detailed in Sections 2 and 3 of this report. In general, sampling locations were selected that would permit comparisons upstream and downstream of NPDES outfalls. In instances where this approach was not feasible because effluents discharge into the headwaters of a stream, appropriate unimpacted reference were used for comparison purposes. This report summarizes the results of the sampling that was conducted in 2000 and also compares these data to the data that were collected in 1997 and 1998.
Date: June 20, 2001
Creator: Specht, W.L.
Partner: UNT Libraries Government Documents Department

Results of Water and Sediment Toxicity Tests and Chemical Analyses Conducted at the Central Shops Burning Rubble Pit Waste Unit, January 1999

Description: The Central Shops Burning Rubble Pit Operable Unit consists of two inactive rubble pits (631-1G and 631-3G) that have been capped, and one active burning rubble pit (631-2G), where wooden pallets and other non-hazardous debris are periodically burned. The inactive rubble pits may have received hazardous materials, such as asbestos, batteries, and paint cans, as well as non-hazardous materials, such as ash, paper, and glass. In an effort to determine if long term surface water flows of potentially contaminated water from the 631-1G, 631-3G, and 631-2G areas have resulted in an accumulation of chemical constituents at toxic levels in the vicinity of the settling basin and wetlands area, chemical analyses for significant ecological preliminary constituents of concern (pCOCs) were performed on aqueous and sediment samples. In addition, aquatic and sediment toxicity tests were performed in accordance with U.S. EPA methods (U.S. EPA 1989, 1994). Based on the results of the chemical analyses, unfiltered water samples collected from a wetland and settling basins located adjacent to the CSBRP Operable Unit exceed Toxicity Reference Values (TRVs) for aluminum, barium, chromium, copper, iron, lead, and vanadium at one or more of the four locations that were sampled. The water contained very high concentrations of clay particles that were present as suspended solids. A substantial portion of the metals were present as filterable particulates, bound to the clay particles, and were therefore not biologically available. Based on dissolved metal concentrations, the wetland and settling basin exceeded TRVs for aluminum and barium. However, the background reference location also exceeded the TRV for barium, which suggests that this value may be too low, based on local geochemistry. The detection limits for both total and dissolved mercury were higher than the TRV, so it was not possible to determine if the TRV for mercury was exceeded. Dissolved ...
Date: June 2, 1999
Creator: Specht, W.L.
Partner: UNT Libraries Government Documents Department

Results of Toxicity Studies Conducted on Outfall X-08 and Its Contributing Waste Streams, November 1999 - June 2000

Description: This interim report summarizes the results of toxicity tests, Toxicity Identification Evaluations, and chemical analyses that have been conducted on SRS's NPDES Outfall X-08 and its contributing waste streams between November 1999 and June 2000.
Date: June 28, 2000
Creator: Specht, W.L.
Partner: UNT Libraries Government Documents Department

Results of chronic toxicity tests conducted on selected A-area outfalls, June-August 1996

Description: In anticipation of possible toxicity testing requirements in the SRS`s new (1996) NPDES permit, toxicity tests were performed at selected A-Area NPDES outfalls in order to determine if the outfalls were toxic. Chronic definitive toxicity tests were conducted on Ceriodaphnia dubia using water collected from nine locations during the summer of 1996. Six of the nine locations were toxic.
Date: July 1, 1997
Creator: Specht, W. L.
Partner: UNT Libraries Government Documents Department

Instream biological assessment of NPDES point source discharges at the Savannah River Site, 1997-1998

Description: The Savannah River Site currently has 33 permitted NPDES outfalls that have been permitted by the South Carolina Department of Health an Environmental Control to discharge to SRS streams and the Savannah River. In order to determine the cumulative impacts of these discharges to the receiving streams, a study plan was developed to perform in-stream assessments of the fish assemblages, macroinvertebrate assemblages, and habitats of the receiving streams.
Date: February 28, 2000
Creator: Specht, W.L.
Partner: UNT Libraries Government Documents Department

Results of toxicity tests and chemical analyses conducted on sediments collected from the TNX Outfall Delta Operable Unit, July 1999

Description: In order to provide unit specific toxicity data that will be used to address critical uncertainty in the ecological risk assessment (ERA) for the TNX Outfall Delta Operable Unit (TNXOD OU), sediments were collected from eight locations in the Inner Swamp portion of the operable unit and two unit specific background locations. These samples were analyzed for total mercury, total uranium, and sediment toxicity.
Date: February 11, 2000
Creator: Specht, W.L.
Partner: UNT Libraries Government Documents Department

Rapid Bioassessment Methods for Assessing Stream Macroinvertebrate Community on the Savannah River Site

Description: Macroinvertebrate sampling was performed at 16 locations in the Savannah River Site (SRS) streams using Hester-Dendy multiplate samplers and EPA Rapid Bioassessment Protocols (RBP). Some of the sampling locations were unimpacted, while other locations had been subject to various forms of perturbation by SRS activities. In general, the data from the Hester-Dendy multiplate samplers were more sensitive at detecting impacts than were the RBP data. We developed a Biotic Index for the Hester-Dendy data which incorporated eight community structure, function, and balance parameters. when tested using a data set that was unrelated to the data set that was used in developing the Biotic Index, the index was very successful at detecting impact.
Date: November 22, 1999
Creator: Specht, W.L.
Partner: UNT Libraries Government Documents Department

Qualitative Macroinvertebrate Assessment of Crouch Branch, June 1999

Description: An assessment of the macroinvertebrate community of Crouch Branch was performed in June 1999 to determine if effluent from the H-02 outfall is impairing the quality of the stream. Concurrent samples were collected for metals analyses (copper and zinc). The results of the study indicate that the stream is most impaired just downstream from the H-02 outfall and that the quality of the stream biota improves with increasing distance from the outfall. Conversely, macroinvertebrate habitat quality is best just downstream from the H-02 outfall. The midreaches of the stream contain very poor habitat quality, and the lower reaches of the stream, contain habitat of intermediate quality. Although much of the stream has degraded habitat due to channel erosion and scouring, there is strong evidence to suggest that the impairment is due to elevated concentrations of copper and zinc that are present in the H-02 effluent. A comparison of macroinvertebrate data collected in 1997 to the data collected in this study indicates that the macroinvertebrate community of Crouch Branch has improved markedly in the last two years.
Date: November 5, 1999
Creator: Specht, W.L.
Partner: UNT Libraries Government Documents Department

Results of Toxicity Tests on SRS NPDES Outfalls A-01, A-11, and G-10, January - September 2002

Description: The results of the 10 rounds of toxicity tests that were performed on SRS NPDES outfalls A-01, A-11 and G-10 indicate that there would have been no toxicity failures if these tests had been compliance tests. Except for one set of test results for G-10, all of the tests resulted in NOECs of 100 percent effluent. All of the tests met the criteria of less than a 25 percent mean reduction in reproduction for any quarter and less than a 40 percent reduction in any single test. Therefore, there is no reasonable potential to discharge toxic effluents in toxic amounts.
Date: November 1, 2002
Creator: Specht, W.L.
Partner: UNT Libraries Government Documents Department

F/H Area ETF effluent (H-016 outfall), ceriodaphnia survival/reproduction test, test date: March 21, 1991

Description: This toxicity test was conducted to determine if the effluent from the F/H area at Savannah River Plant affects the survival or reproduction of the test organisms during a seven day period. The test involved exposing the test organisms to a series of dilutions of the effluent. At each dilution the survival and reproduction of ten test organisms was recorded. Each effluent dilution was compared to a control set of test organisms. Survival data were analyzed by Fisher's Exact Test and the Trimmed Spearman-Karber test to determine the effluent concentration necessary to cause statistically significant (p = 0.05) mortality. Reproduction data was analyzed for normality, homogeneity of variance and equality of replicates among dilutions to determine the appropriate statistical test for analysis of statistical differences in reproduction among dilutions. Results are summarized.
Date: August 1, 1991
Creator: Specht, W.L.
Partner: UNT Libraries Government Documents Department

F/H Area ETF effluent (H-016 Outfall) ceriodaphnia survival/reproduction test, Test date: September 18, 1990

Description: This toxicity test was conducted to determine if the effluent from the F/H area at Savannah River Plant affects the survival or reproduction of the test organisms during a seven day period. The test involved exposing the test organisms (Ceriodaphnia) to a series of dilutions of the effluent. At each dilution the survival and reproduction of ten test organisms was recorded. Each effluent dilution was compared to a control set of test organisms. Survival data were analyzed by Fisher's Exact Test and Trimmed Spearman Karber Analysis to determine the effluent concentration necessary to cause statistically significant (p=0.5) mortality. Reproduction data was analyzed for normality, homogeneity of variance and equality of replicates among distribution to determine the appropriate statistical test for analysis of statistical differences in reproduction among dilutions.
Date: August 1, 1991
Creator: Specht, W.L.
Partner: UNT Libraries Government Documents Department

F/H Area for ETF effluent (H-016 outfall), ceriodaphnia survival/reproduction test, test date: June 27, 1991

Description: This toxicity test was conducted to determine if the effluent from the F/H area at Savannah River Plant affects the survival or reproduction of the test organisms during a seven day period. The test involved exposing the test organisms to a series of dilutions of the effluent. At each dilution the survival and reproduction of ten tests organisms was recorded. Each effluent dilution was compared to a control set of test organisms. Survival data were analyzed by Fisher's Exact Test and Probit Analysis (or Trimmed Spearman Karber if Probit can not be used) to determine the effluent concentration necessary to cause statistically significant (p = 0.05) mortality. Reproduction data was analyzed for normality, homogeneity of variance and equality of replicates among dilutions to determine the appropriate statistical test for analysis of statistical differences among dilutions. Results are summarized.
Date: January 1, 1991
Creator: Specht, W.L.
Partner: UNT Libraries Government Documents Department

F/H Area ETF effluent (H-016 outfall) ceriodaphnia survival/reproduction test, test date: September 21, 1989

Description: This toxicity test was conducted to determine if the effluent from the F/H area of Savannah River Plant causes death (acute toxicity) or reduction in the reproduction of the test organisms (chronic toxicity) during a seven day period. A series of dilutions of the effluent are set to determine how much the effluent must be diluted before toxic effects are no longer noted. Acute toxicity is checked by statistically analyzing whether significantly more organisms die in the effluent dilutions than in the control treatment, and, if significantly more die, how much the effluent must be diluted so as to kill only 50% of the test organisms (the LC50). Chronic toxicity is checked by statistically analyzing whether significantly fewer young are produced by test organisms exposed to the effluent dilutions. Results indicate the lowest effluent concentration which shows a toxic effect (the LOEC) and the highest effluent concentration which does not demonstrate an effect (NOEC). Results are discussed.
Date: August 1, 1991
Creator: Specht, W.L.
Partner: UNT Libraries Government Documents Department

Biological monitoring of Upper Three Runs Creek, Savannah River Plant, Aiken County, South Carolina

Description: In anticipation of the fall 1988 start up of effluent discharges into Upper Three Creek by the F/H Area Effluent Treatment Facility of the Savannah River Site, Aiken, SC, a two and one half year biological study was initiated in June 1987. Upper Three Runs Creek is an intensively studied fourth order stream known for its high species richness. Designed to assess the potential impact of F H area effluent on the creek, the study includes qualitative and quantitative macroinvertebrate stream surveys at five sites, chronic toxicity testing of the effluent, water chemistry and bioaccumulation analysis. This final report presents the results of both pre-operational and post-operational qualitative and quantitative (artificial substrate) macroinvertebrate studies. Six quantitative and three qualitative studies were conducted prior to the initial release of the F/H ETF effluent and five quantitative and two qualitative studies were conducted post-operationally.
Date: October 1, 1991
Creator: Specht, W.L.
Partner: UNT Libraries Government Documents Department

F/H Area ETF effluent (H-016 outfall) ceriodaphnia survival/reproduction test, test date: December 28, 1989

Description: This toxicity test was conducted to determine if the effluent from the H/F area of Savannah River Plant affect the survival or reproduction of the test organisms during a seven day period. The test involved exposing the test organisms to a series of dilutions of the effluent. At each dilution the survival and reproduction of ten test organisms was recorded. Each effluent dilution was compared to a control set of test organisms. Survival data were analyzed by Fisher's Exact Test and Probit Analysis to determine the effluent concentration necessary to cause statistically significant (p=0.05) mortality. Reproduction data was analyzed for normality, homogeneity of variance and equality of replicates among dilutions to determine the appropriate statistical test for analysis of statistical differences in reproduction among dilutions. Results are summarized.
Date: August 1, 1991
Creator: Specht, W.L.
Partner: UNT Libraries Government Documents Department

Results of the F/H Effluent Treatment Facility biological monitoring program, July 1987--July 1991

Description: As required by the South Carolina Department of Health and Environmental Control (SCDHEC) under NPDES Permit SCO000175, biological monitoring was conducted in Upper Three Runs Creek to determine if discharges from the F/H Effluent Treatment Facility have adversely impacted the biotic community of the receiving stream. Data included in this summary report encompass July 1987 through July 1991. As originally designed, the F/H ETF was not expected to remove all of the mercury from the wastewater; therefore, SCDHEC specified that studies be conducted to determine if mercury was bioaccumulating in aquatic biota. Subsequent to approval of the biological monitoring program, an ion exchange column was added to the F/H ETF specifically to remove mercury, which eliminated mercury from the F/H ETF effluent. The results of the biological monitoring program indicate that at the present rate of discharge, the F/H ETF effluent has not adversely affected the receiving stream with respect to any of the parameters that were measured. The effluent is not toxic at the in-stream waste concentration and there is no evidence of mercury bioaccumulation.
Date: July 1, 1992
Creator: Specht, W.L.
Partner: UNT Libraries Government Documents Department

Biological monitoring of Upper Three Runs Creek, Savannah River Plant, Aiken County, South Carolina. Final report on macroinvertebrate stream assessments for F/H area ETF effluent discharge, July 1987--February 1990

Description: In anticipation of the fall 1988 start up of effluent discharges into Upper Three Creek by the F/H Area Effluent Treatment Facility of the Savannah River Site, Aiken, SC, a two and one half year biological study was initiated in June 1987. Upper Three Runs Creek is an intensively studied fourth order stream known for its high species richness. Designed to assess the potential impact of F?H area effluent on the creek, the study includes qualitative and quantitative macroinvertebrate stream surveys at five sites, chronic toxicity testing of the effluent, water chemistry and bioaccumulation analysis. This final report presents the results of both pre-operational and post-operational qualitative and quantitative (artificial substrate) macroinvertebrate studies. Six quantitative and three qualitative studies were conducted prior to the initial release of the F/H ETF effluent and five quantitative and two qualitative studies were conducted post-operationally.
Date: October 1, 1991
Creator: Specht, W. L.
Partner: UNT Libraries Government Documents Department

Results of Macroinvertebrate Sampling Conducted at 33 SRS Stream Locations, July--August 1993

Description: In order to assess the health of the macroinvertebrate communities of SRS streams, the macroinvertebrate communities at 30 stream locations on SRS were sampled during the summer of 1993, using Hester-Dendy multiplate samplers. In addition, three off-site locations in the Upper Three Runs drainage were sampled in order to assess the potential for impact from off-site activities. In interpreting the data, it is important to recognize that these data were from a single set of collections. Macroinvertebrate communities often undergo considerable temporal variation, and are also greatly influenced by such factors as water depth, water velocity, and available habitat. These stations were selected with the intent of developing an on-going sampling program at a smaller number of stations, with the selection of the stations to be based largely upon the results of this preliminary sampling program. When stations within a given stream showed similar results, fewer stations would be sampled in the future. Similarly, if a stream appeared to be perturbed, additional stations or chemical analyses might be added so that the source of the perturbation could be identified. In general, unperturbed streams will contain more taxa than perturbed streams, and the distribution of taxa among orders or families will differ. Some groups of macroinvertebrates, such as Ephemeroptera (mayflies), Plecoptera (stoneflies) and Trichoptera (caddisflies), which are collectively called EPT taxa, are considered to be relatively sensitive to most kinds of stream perturbation; therefore a reduced number of EPT taxa generally indicates that the stream has been subject to chemical or physical stressors. In coastal plain streams, EPT taxa are generally less dominant than in streams with rocky substrates, while Chironomidae (midges) are more abundant. (Abstract Truncated)
Date: December 1, 1994
Creator: Specht, W. L.
Partner: UNT Libraries Government Documents Department

Results of acute and chronic toxicity tests conducted at SRS NPDES outfalls, July--October 1991

Description: Acute (48 hour LC50) and chronic (7-day reproductive impairment) toxicity tests were conducted on Ceriodaphnia dubia in water collected from 53 NPDES outfalls. All tests were conducted at the in-stream waste concentration. only 12 of the 53 outfalls showed no evidence of toxicity. Twenty-eight of the outfalls were acutely toxic, often producing 100% mortality during the first day of exposure. Fourteen outfalls had no discharge at the time of sampling and could not be tested. Three outfalls were not tested because their toxicity has been adequately characterized in other investigations. Elevated concentrations of total residual chlorine are suspected to be responsible for the observed toxicity of many NPDES outfalls, particularly the sanitary wastewater treatment plants. Chemical data from previous studies indicate that metals may also be present in toxic concentrations at many outfalls. Toxicity identification and reduction options are discussed.
Date: January 1, 1992
Creator: Specht, W. L.
Partner: UNT Libraries Government Documents Department

F/H Area ETF effluent (H-016 Outfall) ceriodaphnia survival/reproduction test, Test date: September 18, 1990

Description: This toxicity test was conducted to determine if the effluent from the F/H area at Savannah River Plant affects the survival or reproduction of the test organisms during a seven day period. The test involved exposing the test organisms (Ceriodaphnia) to a series of dilutions of the effluent. At each dilution the survival and reproduction of ten test organisms was recorded. Each effluent dilution was compared to a control set of test organisms. Survival data were analyzed by Fisher`s Exact Test and Trimmed Spearman Karber Analysis to determine the effluent concentration necessary to cause statistically significant (p=0.5) mortality. Reproduction data was analyzed for normality, homogeneity of variance and equality of replicates among distribution to determine the appropriate statistical test for analysis of statistical differences in reproduction among dilutions.
Date: August 1, 1991
Creator: Specht, W. L.
Partner: UNT Libraries Government Documents Department