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Microtechnology

Description: Research reported in the thrust area of microtechnology includes: advanced plasma etch processes for high-aspect-ratio, submicron-feature-size applications; integration of PCR amplification and capillary electrophoresis in a DNA analysis device; microactuators for optical interferometry; thin silicon windows; eutectic bonding and fusion bonding; solid-source MBE-grown GaAs/AlGaAs ridge-waveguide semiconductor optical amplifiers; large area lithography; phase-shift lithography; thermally robust … more
Date: February 1, 1997
Creator: Mariella, R.P.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

The Journey to Fielded BioInstrumentation

Description: Over the last ten years, a team at Lawrence Livermore National Laboratory created fieldable instruments that performed identification/quantification via bioassays. These instruments have been based on molecular surface-recognition assays, such as immunoassays, and on nucleic-acid-based assays, such as the polymerase chain reaction. In 1996, we participated in the Joint Field Trials 3, employing both immunoassays as well as the polymerase chair reaction. In 1998, we participated in the Joint Fie… more
Date: February 24, 2005
Creator: Mariella, R., Jr.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Microtechnology for instrumentation

Description: For the last two decades, the majority of research and development at LLNL in microtechnology has focused on photonics devices and bulk micromachining, including miccroelectro-mechanical systems and associated areas. For the last ten years, we have used these capabilities to address our analytical instrumentation needs. Just as the miniature photonics have enable the fabrication of analytical instruments that are either higher performance, smaller, more portable, or are combinations of these. E… more
Date: January 1, 1998
Creator: Mariella, R.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Individualized Medicine

Description: The recent focus of microelectromechanical-systems (MEMS) based instrumentation has largely dealt with increasing the throughput of established processes, including drug screening/drug discovery/combinatorial chemistry, or the miniaturization of accepted bench-top instruments. The miniaturization and automation of procedures that were previously performed manually are included in these activities. We suggest that BioMEMS instrumentation will adopt an additional direction, that of providing info… more
Date: August 29, 2000
Creator: Mariella, R.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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Deterministic, Nanoscale Fabrication of Mesoscale Objects

Description: Neither LLNL nor any other organization has the capability to perform deterministic fabrication of mm-sized objects with arbitrary, {micro}m-sized, 3-dimensional features with 20-nm-scale accuracy and smoothness. This is particularly true for materials such as high explosives and low-density aerogels. For deterministic fabrication of high energy-density physics (HEDP) targets, it will be necessary both to fabricate features in a wide variety of materials as well as to understand and simulate th… more
Date: September 24, 2004
Creator: Mariella, R., Jr.; Shirk, M; Gilmer, G & Rubenchik, A
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Deterministic, Nanoscale Fabrication of Mesoscale Objects

Description: Neither LLNL nor any other organization has the capability to perform deterministic fabrication of mm-sized objects with arbitrary, {micro}m-sized, 3-D features and with 100-nm-scale accuracy and smoothness. This is particularly true for materials such as high explosives and low-density aerogels, as well as materials such as diamond and vanadium. The motivation for this project was to investigate the physics and chemistry that control the interactions of solid surfaces with laser beams and ion … more
Date: December 8, 2004
Creator: Mariella, R., Jr.; Gilmer, J.; Rubenchik, A. & Shirk, M.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Laser-matter Interaction with Submerged Samples

Description: With the long-term goal in mind of investigating if one could possibly design a 'universal solid-sample comminution technique' for debris and rubble, we have studied pulsed-laser ablation of solid samples that were contained within a surrounding fluid. Using pulses with fluences between 2 J and 0.3 J, wavelengths of 351 and 527 nm, and samples of rock, concrete, and red brick, each submerged in water, we have observed conditions in which {micro}m-scale particles can be preferentially generated … more
Date: March 25, 2010
Creator: Mariella, R., Jr.; Rubenchik, A.; Norton, M.; Donohue, G. & Roberts, K.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Improved Bacterial and Viral Recoveries from 'Complex' Samples using Electrophoretically Assisted Acoustic Focusing

Description: Automated front-end sample preparation technologies can significantly enhance the sensitivity and reliability of biodetection assays [1]. We are developing advanced sample preparation technologies for biowarfare detection and medical point-of-care diagnostics using microfluidic systems with continuous sample processing capabilities. Here we report an electrophoretically assisted acoustic focusing technique to rapidly extract and enrich viral and bacterial loads from 'complex samples', applied i… more
Date: March 27, 2008
Creator: Ness, K.; Rose, K.; Jung, B.; Fisher, K. & Mariella, R. P., Jr.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

adendum for )6 report

Description: Prior to using human nasopharyngeal samples, we will use prepared mixtures of viruses with bacteria and eukaryotic cells in our research on microfluidic separation techniques. Some examples of these mixtures are the bacteriophage MS2 with its host bacteria, E. coli, and BSL-1, Risk-Group-1 virus such as fowlpox virus vaccine with its host cell, DF-1, derived from chickens.
Date: February 2, 2007
Creator: Mariella, R.; Borucki, M.; Miles, R.; Claugue, D.; Dougherty, G. & Fisher, K.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Separation of Yeast Cells from MS2 Viruses Using Acoustic Radiation Force

Description: We report a rapid and robust separation of Saccharomyces cerevisiae and MS2 bacteriophage using acoustic focusing in a microfluidic device. A piezoelectric transducer (PZT) generates acoustic standing waves in the microchannel. These standing waves induce acoustic radiation force fields that direct microparticles towards the nodes (i.e., pressure minima) or the anti-nodes (i.e., pressure maxima) of the standing waves depending on the relative compressidensity between the particle and the suspen… more
Date: March 27, 2008
Creator: Jung, B.; Fisher, K.; Ness, K.; Rose, K. A. & Mariella, R. P., Jr.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Advanced microinstrumentation for rapid DNA sequencing and large DNA fragment separation

Description: Our efforts to develop novel technology for a rapid DNA sequencer and large fragment analysis system based upon gel electrophoresis are described. We are using microfabrication technology to build dense arrays of high speed micro electrophoresis lanes that will ultimately increase the sequencing rate of DNA by at least 100 times the rate of current sequencers. We have demonstrated high resolution DNA fragment separation needed for sequencing in polyacrylamide microgels formed in glass microchan… more
Date: January 25, 1995
Creator: Balch, J.; Davidson, J.; Brewer, L.; Gingrich, J.; Koo, J.; Mariella, R. et al.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Oligonucleotide and Long Polymeric DNA Encoding

Description: This report summarizes the work done at Lawrence Livermore National Laboratory for the Oligonucleotide and Long Polymeric DNA Encoding project, part of the Microelectronic Bioprocesses Program at DARPA. The goal of the project was to develop a process by which long (circa 10,000 base-pair) synthetic DNA molecules could be synthesized in a timely and economic manner. During construction of the long molecule, errors in DNA sequence occur during hybridization and/or the subsequent enzymatic proces… more
Date: November 24, 2003
Creator: Miller, E.; Mariella, R. P. Jr.; Christian, A. T.; Gardner, S. N. & Williams, J. M.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Manipulation of DNA for use in microfluidic devices

Description: MEMS microfluidic systems are becoming increasingly popular as a way to integrate sample preparation and biological assays on a single substrate. The resulting reduction in manual operations and reduced reagent use can lead to significant cost savings in performing biological tests. The authors have explored the use of small scale dielectrophoresis and electrophoresis as a way to manipulate DNA for sample preparation in DNA-based assays. The use of electric fields to manipulate DNA is readily a… more
Date: November 18, 1998
Creator: Belgrader, P.; Bettencourt, K.; Davidson, J. C.; Mariella, R. P.; Miles, R.; Nasarabadi, N. S. et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

FY05 LDRD Final Report Molecular Radiation Biodosimetry LDRD Project Tracking Code: 04-ERD-076

Description: In the event of a nuclear or radiological accident or terrorist event, it is important to identify individuals that can benefit from prompt medical care and to reassure those that do not need it. Achieving these goals will maximize the ability to manage the medical consequences of radiation exposure that unfold over a period of hours, days, weeks, years, depending on dose. Medical interventions that reduce near term morbidity and mortality from high but non-lethal exposures require advanced med… more
Date: February 3, 2006
Creator: Jones, I. M.; A.Coleman, M.; Lehmann, J.; Manohar, C. F.; Marchetti, F.; Mariella, R. et al.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Autonomous system for pathogen detection and identification

Description: This purpose of this project is to build a prototype instrument that will, running unattended, detect, identify, and quantify BW agents. In order to accomplish this, we have chosen to start with the world� s leading, proven, assays for pathogens: surface-molecular recognition assays, such as antibody-based assays, implemented on a high-performance, identification (ID)-capable flow cytometer, and the polymerase chain reaction (PCR) for nucleic-acid based assays. With these assays, we must integr… more
Date: September 24, 1998
Creator: Belgrader, P.; Benett, W.; Langlois, R.; Long, G.; Mariella, R.; Milanovich, F. et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Femtosecond probe-probe transmission studies of LT-grown GaAs near the band edge

Description: We have studied the near-edge optical response of a LT-grown GaAs sample which was deposited at 300{degrees}C on a Si substrate, and then annealed at 600{degrees}C. The Si was etched away to leave a 1 micron free standing GaAs film. Femtosecond transmission measurements were made using an equal pulse technique at four wavelengths between 825 and 870 nm. For each wavelength we observe both a multipicosecond relaxation time, as well as a shorter relaxation time which is less than 100 femtoseconds. more
Date: December 1, 1993
Creator: Radousky, H. B.; Bello, A. F.; Erskine, D. J.; Dinh, L. N.; Bennahmias, M. J.; Perry, M. D. et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Graded Al sub x Ga sub 1-x as photoconductive devices for high efficiency picosecond optoelectronic switching

Description: Picosecond photoconductivity has been achieved for a variety of semiconductor materials by techniques which have now become almost standard. Enhanced scattering by the excessive amount of deep level defects which provide for picosecond recombination lifetimes significantly reduce the mobility, degrading the responsivity of the photoconductor. This paper will present a concept where improved responsivity is achievable by utilizing a graded bandgap Al{sub x}Ga{sub 1-x}As active detecting layer gr… more
Date: October 1, 1990
Creator: Morse, J.D.; Mariella, R.P. (Lawrence Livermore National Lab., CA (USA)) & Dutton, R.W. (Stanford Univ., CA (USA). Center for Integrated Systems)
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Mesoscale Laser Processing using Excimer and Short-Pulse Ti: Sapphire Lasers

Description: Targets to study high-energy density physics and inertial confinement fusion processes have very specific and precise tolerances that are pushing the state-of-the-art in mesoscale microsculpting technology. A significant effort is required in order to advance the capabilities to make these targets with very challenging geometries. Ultrashort pulsed (USP) Ti:Sapphire lasers and excimer lasers are proving to be very effective tools in the fabrication of the very small pieces that make up these ta… more
Date: July 28, 2003
Creator: Shirk, M. D.; Rubenchik, A. M.; Gilmer, G. H.; Stuart, B. C.; Armstrong, J. P.; Oberhelman, S. K. et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

APDS: Autonomous Pathogen Detection System

Description: An early warning system to counter bioterrorism, the Autonomous Pathogen Detection System (APDS) continuously monitors the environment for the presence of biological pathogens (e.g., anthrax) and once detected, it sounds an alarm much like a smoke detector warns of a fire. Long before September 11, 2001, this system was being developed to protect domestic venues and events including performing arts centers, mass transit systems, major sporting and entertainment events, and other high profile si… more
Date: February 14, 2002
Creator: Langlois, R. G.; Brown, S.; Burris, L.; Colston, B.; Jones, L.; Makarewicz, T. et al.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

FY10 Engineering Innovations, Research and Technology Report

Description: This report summarizes key research, development, and technology advancements in Lawrence Livermore National Laboratory's Engineering Directorate for FY2010. These efforts exemplify Engineering's nearly 60-year history of developing and applying the technology innovations needed for the Laboratory's national security missions, and embody Engineering's mission to ''Enable program success today and ensure the Laboratory's vitality tomorrow.'' Leading off the report is a section featuring compelli… more
Date: January 11, 2011
Creator: Lane, M. A.; Aceves, S. M.; Paulson, C. N.; Candy, J. V.; Bennett, C. V.; Carlisle, K. et al.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

FY06 Engineering Research and Technology Report

Description: This report summarizes the core research, development, and technology accomplishments in Lawrence Livermore National Laboratory's Engineering Directorate for FY2006. These efforts exemplify Engineering's more than 50-year history of developing and applying the technologies needed to support the Laboratory's national security missions. A partner in every major program and project at the Laboratory throughout its existence, Engineering has prepared for this role with a skilled workforce and techn… more
Date: January 22, 2007
Creator: Minichino, C.; Alves, S. W.; Anderson, A. T.; Bennett, C. V.; Brown, C. G.; Brown, W. D. et al.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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