Search Results

Advanced search parameters have been applied.
open access

SMEAR STUDY OF D205 (CHEMICAL ENGINEERING BUILDING)

Description: The smear study shows that at least 80% of the floorsmear surveys which were made in a clear area of the Chemical Engineering Building during the course of this study indicated radioactivity equal to or less than 10 d/m/ ft/sup 2/ alpha and equal to or less than 200 d/m/ft/sup 2/ beta-gamma. The smear survey technique is excellent for use in a highhazard area, such as a Pu facility. It is capable of detecting as little as 10 d/m/ft/sup 2/ of alpha contamination with a high degree of confidence.… more
Date: July 1, 1962
Creator: Marchetti, F.P.
Partner: UNT Libraries Government Documents Department
open access

Mechanisms and consequences of paternally transmitted chromosomal abnormalities

Description: Paternally transmitted chromosomal damage has been associated with pregnancy loss, developmental and morphological defects, infant mortality, infertility, and genetic diseases in the offspring including cancer. There is epidemiological evidence linking paternal exposure to occupational or environmental agents with an increased risk of abnormal reproductive outcomes. There is also a large body of literature on germ cell mutagenesis in rodents showing that treatment of male germ cells with mutage… more
Date: April 5, 2005
Creator: Marchetti, F & Wyrobek, A J
Partner: UNT Libraries Government Documents Department
open access

Cell-Type-Specific Genome-wide Expression Profiling after Laser Capture Microdissection of Living Tissue

Description: The purpose of this technical feasibility study was to develop and evaluate robust microgenomic tools for investigations of genome-wide expression of very small numbers of cells isolated from whole tissue sections. Tissues contain large numbers of cell-types that play varied roles in organ function and responses to endogenous and exogenous toxicants whether bacterial, viral, chemical or radiation. Expression studies of whole tissue biopsy are severely limited because heterogeneous cell-types re… more
Date: February 9, 2005
Creator: Marchetti, F & Manohar, C F
Partner: UNT Libraries Government Documents Department
open access

Mechanisms and chemical induction of aneuploidy in rodent germ cells

Description: The objective of this review is to suggest that the advances being made in our understanding of the molecular events surrounding chromosome segregation in non-mammalian and somatic cell models be considered when designing experiments for studying aneuploidy in mammalian germ cells. Accurate chromosome segregation requires the temporal control and unique interactions among a vast array of proteins and cellular organelles. Abnormal function and temporal disarray among these, and others to be inid… more
Date: October 15, 2004
Creator: Mailhes, J B & Marchetti, F
Partner: UNT Libraries Government Documents Department
open access

Advances in understanding paternally transmitted Chromosomal Abnormalities

Description: Multicolor FISH has been adapted for detecting the major types of chromosomal abnormalities in human sperm including aneuploidies for clinically-relevant chromosomes, chromosomal aberrations including breaks and rearrangements, and other numerical abnormalities. The various sperm FISH assays have been used to evaluate healthy men, men of advanced age, and men who have received mutagenic cancer therapy. The mouse has also been used as a model to investigate the mechanism of paternally transmitte… more
Date: March 1, 2001
Creator: Marchetti, F; Sloter, E & Wyrobek, A J
Partner: UNT Libraries Government Documents Department
open access

Relative susceptibilities of male germ cells to genetic defects induced by cancer chemotherapies

Description: Some chemotherapy regimens include agents that are mutagenic or clastogenic in model systems. This raises concerns that cancer survivors, who were treated before or during their reproductive years, may be at increased risks for abnormal reproductive outcomes. However, the available data from offspring of cancer survivors are limited, representing diverse cancers, therapies, time-to-pregnancies, and reproductive outcomes. Rodent breeding data after paternal exposures to individual chemotherapeut… more
Date: June 15, 2004
Creator: Wyrobek, A J; Schmid, T E & Marchetti, F
Partner: UNT Libraries Government Documents Department
open access

Impaired fertility in T-stock female mice after superovulation

Description: Superovulation of female mice with exogenous gonadotrophins is routinely used for increasing the number of eggs ovulated by each female in reproductive and developmental studies. We report an unusual effect of superovulation on fertilization in mice. In vivo matings of superovulated T-stock females with B6C3F1 males resulted in a 2-fold reduction (P<0.001) in the frequencies of fertilized eggs compared to control B6C3F1 matings. In addition, {approx}22 hr after mating only 15% of fertilized … more
Date: December 5, 2003
Creator: Wyrobek, A J; Bishop, J B; Marchetti, F & Zudova, D
Partner: UNT Libraries Government Documents Department
open access

ETOPOSIDE INDUCES CHROMOSOMAL ABNORMALITIES IN SPERMATOCYTES AND SPERMATOGONIAL STEM CELLS

Description: Etoposide (ET) is a chemotherapeutic agent widely used in the treatment of leukemia, lymphomas and many solid tumors, such as testicular and ovarian cancers, that affect patients in their reproductive years. The purpose of the study was to use sperm FISH analyses to characterize the long-term effects of ET on male germ cells. We used a mouse model to characterize the induction of chromosomal aberrations (partial duplications and deletions) and whole chromosomal aneuploidies in sperm of mice tre… more
Date: July 15, 2005
Creator: Marchetti, F; Pearson, F S; Bishop, J B & Wyrobek, A J
Partner: UNT Libraries Government Documents Department
open access

The Effects of Male Age on Sperm DNA Damage in Healthy Non-Smokers

Description: The trend for men to have children at older ages raises concerns that advancing age may increase the production of genetically defective sperm, increasing the risks of transmitting germ-line mutations. We investigated the associations between male age and sperm DNA damage and the influence of several lifestyle factors in a healthy non-clinical group of 80 non-smokers (age: 22-80) with no known fertility problems using the sperm Comet analyses. The average percent of DNA that migrated out of the… more
Date: March 8, 2006
Creator: Schmid, T.; Eskenazi, B.; Baumgartner, A.; Marchetti, F.; Young, S.; Weldon, R. et al.
Partner: UNT Libraries Government Documents Department
open access

Detection of structural and numerical chomosomal abnormalities by ACM-FISH analysis in sperm of oligozoospermic infertility patients

Description: Modern reproductive technologies are enabling the treatment of infertile men with severe disturbances of spermatogenesis. The possibility of elevated frequencies of genetically and chromosomally defective sperm has become an issue of concern with the increased usage of intracytoplasmic sperm injection (ICSI), which can enable men with severely impaired sperm production to father children. Several papers have been published about aneuploidy in oligozoospermic patients, but relatively little is k… more
Date: November 10, 2003
Creator: Schmid, T. E.; Brinkworth, M. H.; Hill, F.; Sloter, E.; Kamischke, A.; Marchetti, F. et al.
Partner: UNT Libraries Government Documents Department
open access

Low Dose Radiation Response Curves, Networks and Pathways in Human Lymphoblastoid Cells Exposed from 1 to 10 cGy of Acute Gamma Radiation

Description: We investigated the low dose dependency of the transcriptional response of human cells to characterize the shape and biological functions associated with the dose response curve and to identify common and conserved functions of low dose expressed genes across cells and tissues. Human lymphoblastoid (HL) cells from two unrelated individuals were exposed to graded doses of radiation spanning the range of 1-10 cGy were analyzed by transcriptome profiling, qPCR and bioinformatics, in comparison to … more
Date: April 18, 2011
Creator: Wyrobek, A. J.; Manohar, C. F.; Nelson, D. O.; Furtado, M. R.; Bhattacharya, M. S.; Marchetti, F. et al.
Partner: UNT Libraries Government Documents Department
open access

FY05 LDRD Final Report Molecular Radiation Biodosimetry LDRD Project Tracking Code: 04-ERD-076

Description: In the event of a nuclear or radiological accident or terrorist event, it is important to identify individuals that can benefit from prompt medical care and to reassure those that do not need it. Achieving these goals will maximize the ability to manage the medical consequences of radiation exposure that unfold over a period of hours, days, weeks, years, depending on dose. Medical interventions that reduce near term morbidity and mortality from high but non-lethal exposures require advanced med… more
Date: February 3, 2006
Creator: Jones, I. M.; A.Coleman, M.; Lehmann, J.; Manohar, C. F.; Marchetti, F.; Mariella, R. et al.
Partner: UNT Libraries Government Documents Department
Back to Top of Screen