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Phage Hunters Advancing Genomics and Evolutionary Science (PHAGES): Implementing a Research-based Course for Freshmen at the University of North Texas

Description: Paper describes the Phage Hunters Advancing Genomics and Evolutionary Science (PHAGES) program from the Howard Hughes Medical Institute’s Science Education Alliance as implemented at the University of North Texas.
Date: 2012
Creator: Hughes, Lee E. & Benjamin, Robert C.
Item Type: Refine your search to only Article
Partner: UNT Libraries
open access

Comparison and Genetic Analysis of Host Specificity in Cluster BD1 Bacteriophages infecting Streptomyces

Description: Bacteriophages are viruses that specifically infect bacteria. When a phage infects a bacterium, it attaches itself to the surface of the bacteria and injects its DNA into the intracellular space. The phage DNA hijacks the cellular machinery of the bacteria and forces it to produce phage proteins. Eventually, the bacteria cell bursts or lyses, releasing new phage. The bacteria act as a host for phage reproduction. The ability for a phage to infect multiple bacterial species is known as host rang… more
Date: May 2019
Creator: Klug, Hannah
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Exploration of Genome Length, Burst Time, and Burst Size of Streptomyces griseus Bacteriophages

Description: Since phages use the host resources to replicate themselves after infection, the different sizes of the phage genome should influence the replication rate. We, therefore, hypothesized that the smaller genomes should burst the cell faster than the larger ones. As well, the shorter genomes would have greater burst sizes because they should replicate faster. Here, we obtained 16 phages of various genome length. All phages were isolated on Streptomyces griseus and available in our phage bank at the… more
Date: May 2019
Creator: Maneekul, Jindanuch
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Isolation and Characterization of Phages Infecting Streptomyces azureus

Description: Isolating novel phages using Streptomyces azureus, which produces antibiotic thiostrepton, as a host, and characterizing the genomes may help us to find new tools that could be used to develop antibiotics in addition to contribute to the databases of phages and specifically, Streptomyces phages. Streptomyces phages Alsaber, Omar, Attoomi, Rowa, and ZamZam were isolated using during this study. They were isolated from enriched soil and sequenced by Illumina sequencing method. They were isolated … more
Date: May 2018
Creator: Sulaiman, Ahmad M.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Regulation of Alternative Sigma Factors During Oxidative and Ph Stresses in the Phototroph Rhodopseudomonas Palustris

Description: Rhodopseudomonas palustris is a metabolically versatile phototrophic α-proteobacterium. The organism experiences a wide range of stresses in its environment and during metabolism. The oxidative an pH stresses of four ECF (extracytoplasmic function) σ-factors are investigated. Three of these, σ0550, σ1813, and σ1819 show responses to light-generated singlet oxygen and respiration-generated superoxide reactive oxygen species (ROS). The EcfG homolog, σ4225, shows a high response to superoxide and … more
Date: August 2014
Creator: Perry, Leslie M.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Pyrimidine Metabolism in Streptomyces griseus

Description: Salvage of pyrimidine nucleosides and bases by S. griseus and the regulation of aspartate transcarbamoylase (ATCase) were studied. The velocity-substrate curve for S. griseus ATCase was hyperbolic for both aspartate and carbamoylphosphate. The enzyme activity was diminished in the presence of ATP, CTP, or UTP. The synthesis of ATCase was repressed in cells grown in the presence of exogenous uracil. The specific activity of cells grown with uracil was 43 percent of that for cells grown in minima… more
Date: August 1994
Creator: Hughes, Lee E. (Lee Everette)
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

The Microbial Retting Environment of Hibiscus Cannabinus and Its Implications in Broader Applications

Description: Fiber-yielding plants is an area of increased interest due to the potential use in a variety of green-based materials. These biocomposites can be incorporated into multiple uses; for example, to replace building materials and interior vehicular paneling. The research here aims to focus in on the crop Hibiscus cannabinus for utilization into these functions. H. cannabinus is economically attractive due to the entire process being able to be accomplished here in the United States. The plant can b… more
Date: May 2015
Creator: Visi, David K.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries

Isolation and Characterization of Novel Microbacteriophage Barbara

Description: Poster presentation for the 2010 University Scholars Day at the University of North Texas discussing research on the isolation of a new strain of mycobacteriophage from soil collection using a Mycobacterium smegmatis host strain and an enrichment protocol.
Date: April 15, 2010
Creator: Mitchell, Angel; Simon, Stephanie E.; Benjamin, Robert C. & Hughes, Lee E.
Item Type: Refine your search to only Poster
Partner: UNT Honors College
open access

Identification of Genes Involved in Flocculation by Whole Genome Sequencing of Thauera aminoaromatica Strain MZ1T Floc-defective Mutants

Description: Thauera aminoaromatica MZ1T, a floc-forming bacterium isolated from an industrial activated sludge wastewater treatment plant, overproduces exopolysaccharide (EPS) leading to viscous bulking. This phenomenon results in poor sludge settling and dewatering during the clarification process. To identify genes responsible for bacterial flocculation, a whole genome phenotypic sequencing technique was applied. Genomic DNA of MZ1T flocculation-deficient mutants were subjected to massively parallel sequ… more
Date: December 2015
Creator: Prombutara, Pinidphon
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Isolation and Genomic Characterization of 45 Novel Bacteriophages Infecting the Soil Bacterium Streptomyces griseus

Description: Bacteriophages, or simply "phages," are the most abundant biological entities on the planet and are thought to be the largest untapped reservoir of available genetic information. They are also important contributors to both soil health and nutrient recycling and have significantly influenced our current understanding of molecular biology. Bacteria in the genus Streptomyces are also known to be important contributors to soil health, as well as producing a number of useful antibiotics. The geneti… more
Date: December 2018
Creator: Hale, Richard
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

G-RISE at the University of North Texas

Description: Data management plan for the grant "G-RISE at the University of North Texas." The University of North Texas (UNT) serves over 32,000 undergraduate and 7,000 graduate students of which 50% of the undergraduate population and 20% of the graduate population are underrepresented minority (URM). UNT is ranked by the Carnegie Classification as a Tier 1 institute, is a Hispanic Serving Institute, and is dedicated to providing quality mentoring to a diverse group of Ph.D. students. The U… more
Date: 2021-05-01/2022-04-30
Creator: Padilla, Pamela A.; Burggren, Warren W.; Cisneros, Gerardo Andrés & Hughes, Lee E.
Item Type: Refine your search to only Text
Partner: UNT College of Science
open access

The Effectiveness of Hybrid Problem-Based Learning versus Manual-Based Learning in the Microbiology Laboratory

Description: Promising results from the use of problem-based learning (PBL) as a teaching method in medical programs have encouraged many institutions to incorporate PBL into their curricula. This study investigates how applying hybrid-PBL (H-PBL) in a microbiology laboratory impacts students' higher-order thinking as compared to applying a lecture-based pedagogy. The experimental design compared the learning outcomes of two groups of students: the control group and the H-PBL group, for whom PBL cases compr… more
Date: May 2017
Creator: Alharbi, Najwa
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Purification and Analysis of Mycobacteriophage Alice

Description: This paper discusses research on the purification and analysis of mycobacteriophage Alice. The purpose of this research is to expand the knowledge of mycobacteriophage and analyze a single mycobacteriophage genome to be archived for future use.
Date: April 15, 2010
Creator: Manley, Coreen M.; Simon, Stephanie E.; Benjamin, Robert C. & Hughes, Lee E.
Item Type: Refine your search to only Paper
Partner: UNT Honors College
open access

Cloacal Microbiota of Captive-bred and Wild Attwater’s Prairie-chicken, Tympanuchus Cupido Attwateri

Description: The Attwater’s prairie-chicken (Tympanuchus cupido attwateri; APC) is a species of grouse native to Texas coastal prairies and is on the critically endangered species list as a result of habitat destruction and overhunting. All of the current populations were captively bred and released into the wild. Survivorship for released APCs is very low, and individuals seldom survive to reproduce in the wild. One factor contributing to this may be an alteration in the gut microbiota as a result of ca… more
Date: August 2014
Creator: Simon, Stephanie E.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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