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Microbial activity at Yucca Mountain

Description: The U.S. Department of Energy is engaged in a suitability study for a potential geological repository at Yucca Mountain, Nevada, for the containment and storage of commercially generated spent fuel and defense high-level nuclear waste. There is growing recognition of the role that biotic factors could play in this repository, either directly through microbially induced corrosion (MIC), or indirectly by altering the chemical environment or contributing to the transport of radionuclides. As a fir… more
Date: September 25, 1995
Creator: Horn, J.M. & Meike, A.
Partner: UNT Libraries Government Documents Department
open access

Evidence of biogenic corrosion of titanium after exposure to a continuous culture of thiobacillus ferrooxidans grown in thiosulfate medium

Description: Experiments were undertaken to evaluate extreme conditions under which candidate materials intended for use in a proposed nuclear waste repository might be susceptible to corrosion by endogenous microorganisms. Thiobucillus ferrooxidans, a sulfur-oxidizing bacterium, was grown in continuous culture using thiosulfate as an energy source; thiosulfate is oxidized to sulfate as a metabolic endproduct by this organism. Culture conditions were optimized to produce a high-density, metabolically active… more
Date: December 7, 2000
Creator: Horn, J M; Martin, S I & Masterson, B
Partner: UNT Libraries Government Documents Department
open access

Assessing microbiologically induced corrosion of waste package materials in the Yucca Mountain repository

Description: The contribution of bacterial activities to corrosion of nuclear waste package materials must be determined to predict the adequacy of containment for a potential nuclear waste repository at Yucca Mountain (YM), NV. The program to evaluate potential microbially induced corrosion (MIC) of candidate waste container materials includes characterization of bacteria in the post-construction YM environment, determination of their required growth conditions and growth rates, quantitative assessment of … more
Date: January 1, 1998
Creator: Horn, J. M., LLNL
Partner: UNT Libraries Government Documents Department
open access

Initial studies to assess microbial impacts on nuclear waste disposal

Description: The impacts of the native and introduced bacteria on the performance of geologic nuclear waste disposal facilities should be evaluated because these bacteria could promote corrosion of repository components and alteration of chemical and hydrological properties of the surrounding engineered and rock barriers. As a first step towards investigating these potentialities, native and introduced bacteria obtained from post-construction Yucca Mountain (YM) rock were isolated under varying conditions, … more
Date: February 20, 1996
Creator: Horn, J.M.; Meike, A.; McCright, R.D. & Economides, B.
Partner: UNT Libraries Government Documents Department
open access

MIC evaluation and testing for the Yucca Mountain repository

Description: The U.S. Department of Energy is engaged in a suitability study for a potential deep geological repository at Yucca Mountain (YM), Nevada, for the containment and storage of high-level nuclear waste. There is growing awareness that biotic factors could affect the integrity of the repository directly through microbially induced corrosion (MIC) of waste package (WP) materials and other repository elements. A program to determine the degree that microorganisms, especially bacteria, influence the c… more
Date: October 1, 1997
Creator: Horn, J. M.; Rivera, A.; Lain, T. & Jones, D. A.
Partner: UNT Libraries Government Documents Department
open access

Sustaining native microbial growth with endogenous nutrients at Yucca Mountain

Description: The integrity of candidate waste package materials for the proposed Yucca Mountain (YM) repository may be compromised by the corrosive activities of microorganisms. Bacterial activities will be dependent on the abilities of deleterious bacteria to grow and multiply in the repository environment. Therefore, preliminary to assessing microbial induced corrosion, experiments were undertaken to determine the growth of native YM bacterial communities in modified YM pore water. Specifically, we sought… more
Date: February 1, 1998
Creator: Davis, M.A.; Martin, S.; Miranda, A. & Horn, J.M.
Partner: UNT Libraries Government Documents Department
open access

Potential Biogenic Corrosion of Alloy 22, A Candidate Nuclear Waste Packaging Materials, Under Simulated Repository Conditions

Description: The U.S. Department of Energy has been charged with assessing the suitability of a geologic nuclear waste repository at Yucca Mountain (YM), NV. Microorganisms, both those endogenous to the repository site and those introduced as a result of construction and operational activities, may contribute to the corrosion of metal nuclear waste packaging and thereby decrease their useful lifetime as barrier materials. Evaluation of potential Microbiological Influenced Corrosion (MIC) on candidate waste … more
Date: January 12, 2000
Creator: Horn, J. M.; Martin, S. I.; Rivera, A. J.; Bedrossian, P. J. & Lian, T.
Partner: UNT Libraries Government Documents Department
open access

Identification of micro-organisms using superconducting tunnel junctions and time-of-flight mass spectrometry

Description: We present time-of-flight measurements of biological material ejected from complete bacterial spores following laser irradiation. Ion impacts are registered on a microchannel plate and on a Superconducting Tunnel Junction (STJ) detector. We compare mass spectra obtained with the two detectors. The STJ has better sensitivity to massive ions and also measures the energy of each ion. We show evidence that spores of different bacillus species produce distinctive mass spectra and associate the obser… more
Date: August 30, 1999
Creator: Benner, W H; Frank, M; Horn, J M; Labov, S E; Langry, K & Ullom, J
Partner: UNT Libraries Government Documents Department
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