3 Matching Results

Search Results

Advanced search parameters have been applied.
in creator/contributor: "Hermanowicz, S. W."  
Results: 1 - 3
open access

Developing a Biosensor for Estrogens in Water Samples: Study ofthe Real-time Response of Live Cells of the Estrogen-sensitive YeastStrain RMY/ER-ERE using Fluorescence Microscopy

Description: Using a fluorescein di-{beta}-d-galactopyranoside (FDG) substrate we show that in live cells of an estrogen-sensitive yeast strain RMY/ER-ERE with human estrogen receptor (ER{alpha}) gene and the lacZ gene which encodes {beta}-galactosidase, the uptake of 17{beta}-estradiol (E2) and the subsequent production of {beta}-galactosidase enzyme occur quite rapidly, with maximal enzyme-catalyzed product formation evident after about 30 min of exposure to E2. This finding which agrees with the well-kno… more
Date: January 1, 2006
Creator: Wozei, E.; Hermanowicz, S. W. & Holman, H-Y.N.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Developing a Biosensor for Estrogens in Water Samples: Study ofthe Real-time Response of Live Cells of the Estrogen-sensitive YeastStrain RMY/ER-ERE using Fluorescence Microscopy

Description: Using a fluorescein di-{beta}-D-galactopyranoside (FDG) substrate we show that in live cells of an estrogen-sensitive yeast strain RMY/ER-ERE with human estrogen receptor (ER{alpha}) gene and the lacZ gene which encodes {beta}-galactosidase, the uptake of 17 {beta}-estradiol (E2) and the subsequent production of {beta}-galactosidase enzyme occur quite rapidly, with maximal enzyme-catalyzed product formation evident after about 30 minutes of exposure to E2. This finding which agrees with the wel… more
Date: July 13, 2005
Creator: Wozei, E.; Hermanowicz, S. W. & Holman, H-Y. N.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Detecting estrogenic activity in water samples withestrogen-sensitive yeast cells using spectrophotometry and fluorescencemicroscopy

Description: Environmental estrogens are environmental contaminants that can mimic the biological activities of the female hormone estrogen in the endocrine system, i.e. they act as endocrine disrupters. Several substances are reported to have estrogen-like activity or estrogenic activity. These include steroid hormones, synthetic estrogens (xenoestrogens), environmental pollutants and phytoestrogens (plant estrogens). Using the chromogenic substrate ortho-nitrophenyl-{beta}-D-galactopyranoside (ONPG) we sh… more
Date: March 15, 2006
Creator: Wozei, E.; Holman, H-Y.N.; Hermanowicz, S.W. & S., Borglin
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
Back to Top of Screen