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open access

Fluorescent CdSe/ZnS nanocrystal-peptide conjugates for long-term, nontoxic imaging and nuclear targeting in living cells

Description: One of the biggest challenges in cell biology is the imaging of living cells. For this purpose, the most commonly used visualization tool is fluorescent markers. However, conventional labels, such as organic fluorescent dyes or green fluorescent proteins (GFP), lack the photostability to allow the tracking of cellular events that happen over minutes to days. In addition, they are either toxic to cells (dyes), or difficult to construct and manipulate (GFP). We report here the use of a new class … more
Date: June 14, 2004
Creator: Chen, Fanqing & Gerion, Daniele
Partner: UNT Libraries Government Documents Department
open access

Optical Properties of CdSe Nanoparticle Assemblies

Description: We report on three-dimensional fluorescence imaging of micron-size faceted crystals precipitated from solutions of CdSe nanocrystals. Such crystals have previously been suggested to be superlattices of CdSe quantum dots [1,2]. Possible applications for these materials include their use in optical and optoelectronic devices. The micron-size crystals were grown by slow evaporation from toluene solutions of CdSe nanocrystals in the range of 3-6 nm, produced by traditional wet-chemistry techniques.… more
Date: November 24, 2003
Creator: Huser, Thomas; Gerion, Daniele; Zaitseva, Natalia; Krol, Denise M. & Leon, F. Rafael
Partner: UNT Libraries Government Documents Department
open access

Sorting fluorescent nanocrystals with DNA

Description: Semiconductor nanocrystals with narrow and tunable fluorescence are covalently linked to oligonucleotides. These biocompounds retain the properties of both nanocrystals and DNA. Therefore, different sequences of DNA can be coded with nanocrystals and still preserve their ability to hybridize to their complements. We report the case where four different sequences of DNA are linked to four nanocrystal samples having different colors of emission in the range of 530-640 nm. When the DNA-nanocrystal… more
Date: December 10, 2001
Creator: Gerion, Daniele; Parak, Wolfgang J.; Williams, Shara C.; Zanchet, Daniela; Micheel, Christine M. & Alivisatos, A. Paul
Partner: UNT Libraries Government Documents Department
open access

Quantum Dots-based Reverse Phase Protein Microarray

Description: CdSe nanocrystals, also called quantum dots (Qdots) are a novel class of fluorophores, which have a diameter of a few nanometers and possess high quantum yield, tunable emission wavelength and photostability. They are an attractive alternative to conventional fluorescent dyes. Quantum dots can be silanized to be soluble in aqueous solution under biological conditions, and thus be used in bio-detection. In this study, we established a novel Qdot-based technology platform that can perform accurat… more
Date: July 15, 2005
Creator: Shingyoji, Masato; Gerion, Daniele; Pinkel, Dan; Gray, Joe W. & Chen, Fanqing
Partner: UNT Libraries Government Documents Department
open access

Semiconductor Quantum Rods as Single Molecule FluorescentBiological Labels

Description: In recent years, semiconductor quantum dots have beenapplied with great advantage in a wide range of biological imagingapplications. The continuing developments in the synthesis of nanoscalematerials and specifically in the area of colloidal semiconductornanocrystals have created an opportunity to generate a next generation ofbiological labels with complementary or in some cases enhanced propertiescompared to colloidal quantum dots. In this paper, we report thedevelopment of rod shaped semicond… more
Date: May 29, 2006
Creator: Fu, Aihua; Gu, Weiwei; Boussert, Benjamine; Koski, Kristie; Gerion, Daniele; Manna, Liberato et al.
Partner: UNT Libraries Government Documents Department
open access

Conjugation of DNA to Silanized Colloidal Semiconductor Nanocrystalline Quantum Dots

Description: Water-soluble, highly fluorescent, silanized semiconductor nanocrystals with different surface charges were synthesized. To covalently attach the nanocrystals to biological macromolecules with a variety of mild coupling chemistries, the outermost siloxane shells were derivatized with thiol, amino, or carboxyl functional groups. Single- or double-stranded DNA was coupled to the nanocrystal surfaces by using commercially available bifunctional cross-linker. Conjugation had little effect on the op… more
Date: September 10, 2001
Creator: Parak, Wolfgang J.; Gerion, Daniele; Zanchet, Daniela; Waerz, Anke S.; Micheel, Christine; Williams, Shara C. et al.
Partner: UNT Libraries Government Documents Department
open access

Nanoplasmonic molecular ruler for nuclease activity and DNAfootprinting

Description: We have constructed a nanoplasmonic molecular ruler, which can perform label-free and real-time monitoring of DNA length changes and perform DNA footprinting. The ruler was created by tethering double-stranded DNA to single Au nanoparticles. The scattering spectra of Au-DNA nanoconjugates showed red-shifted peak plasmon resonance wavelength dependent on DNA length, which can be measured with sub-nanometer axial resolution, averaging {approx}1.24 nm peak wavelength shift per DNA base pair. The s… more
Date: August 15, 2006
Creator: Chen, Fanqing Frank; Liu, Gang L.; Yin, Yadong; Gerion, Daniele; Kunchakarra, Siri; Mukherjee, Bipasha et al.
Partner: UNT Libraries Government Documents Department
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