Description: A novel experimental and computational technique based on multiple enzymatic digestion of a protein or protein mixture that reconstructs protein sequences from sequences of overlapping peptides is described in this SAND report. This approach, analogous to shotgun sequencing of DNA, is to be used to sequence alternative spliced proteins, to identify post-translational modifications, and to sequence genetically engineered proteins.
Date: June 1, 2009
Creator: Faulon, Jean-Loup Michel & Heffelfinger, Grant S.
Item Type: Refine your search to only Report
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