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ENHANCED PRACTICAL PHOTOSYNTHETIC CO2 MITIGATION

Description: This quarterly report documents significant achievements in the Enhanced Practical Photosynthetic CO{sub 2} Mitigation project during the period from 10/2/2003 through 1/1/2004. As indicated in the list of accomplishments below we have seen very encouraging results from the model scale tests in terms of organism growth rates and we have begun the final tests necessary to meet our project goals. Specific results and accomplishments for the fourth quarter of 2003 include: (1) Bioreactor support systems and test facilities--(A) The solar collector is working well and has survived the winter weather. (B) The improved high-flow CRF-2 test system has been used successfully to run several long-term growth tests with periodic harvesting events. The high flow harvesting system performed well. The mass measurement results after a 4-week test show 275% growth over the initial mass loading. This figure would have been higher had there been no leakage and handling losses. Carbon dating of biomass from this test is planned for carbon uptake estimation. The next test will include direct measurement of carbon uptake in addition to organism mass measurements. (C) Qualitative organism growth testing has begun in the pilot scale bioreactor. Some issues with uniformity of organism loading, fluid leakage and evaporation have surfaced and are currently being addressed, and quantitative testing will begin as soon as these problems are resolved. (2) Organisms and Growth Surfaces--(A) Montana State University (Subcontracted to do organism studies) submitted their final (3-year) project report. An abstract of the report in included in this quarterly report.
Date: January 30, 2004
Creator: Kremer, Gregory; Bayless, David J.; Vis, Morgan; Prudich, Michael; Cooksey, Keith & Muhs, Jeff
Partner: UNT Libraries Government Documents Department

ENHANCED PRACTICAL PHOTOSYNTHETIC CO{sub 2} MITIGATION

Description: This quarterly report documents significant achievements in the Enhanced Practical Photosynthetic CO{sub 2} Mitigation project during the period from 1/2/2004 through 4/1/2004. Specific results and accomplishments for the first quarter of 2004 include: (1) CRF-2 test system: After the recent successful test results were achieved, the system was taken off-line for re-sealing and other operational improvements to prepare for the next level of testing, which will include direct measurement of carbon uptake in addition to organism mass measurements. (2) 15 biomass slurry samples are currently being analyzed with carbon dating techniques at Galbraith Labs, and statistical analysis of the results will determine if pre and post test carbon analysis is an acceptable means for carbon uptake estimation. (3) Pilot Scale: Quantitative organism growth testing is underway in the pilot scale bioreactor. Problems with uniformity of organism loading delayed the start of quantitative testing, and it remains as a continuing issue that has not been completely resolved. (4) The sustainability test was begun with approximately 30 gallons of algae and 2 Omnisil membranes. The initial mass determination procedure was completed, and the biomass growth over the course of the experiment has been preliminarily quantified.
Date: April 15, 2004
Creator: Kremer, Gregory; Bayless, David J.; Vis, Morgan; Prudich, Michael; Cooksey, Keith & Muhs, Jeff
Partner: UNT Libraries Government Documents Department

Enhanced Practical Photosynthetic CO2 Mitigation

Description: This report highlights significant achievements in the Enhanced Practical Photosynthetic CO{sub 2} Mitigation Project for the period ending 09/30/2004. The primary effort of this quarter was focused on mass transfer of carbon dioxide into the water film to study the potential effects on the photosynthetic organisms that depend on the carbon. Testing of the carbon dioxide scrubbing capability (mass transfer capability) of flowing water film appears to be relatively high and largely unaffected by transport of the gas through the bioreactor. The implications are that the transfer of carbon dioxide into the film is nearly at maximum and that it is sufficient to sustain photosynthesis at whatever rate the organisms can sustain. This finding is key to assuming that the process is an energy (photon) limited reaction and not a nutrient limited reaction.
Date: October 13, 2004
Creator: Kremer, Gregory; Bayless, David J.; Vis, Morgan; Prudich, Michael; Cooksey, Keith & Muhs, Jeff
Partner: UNT Libraries Government Documents Department

Enhanced Practical Photosynthetic CO2 Mitigation

Description: This quarterly report documents significant achievements in the Enhanced Practical Photosynthetic CO{sub 2} Mitigation project during the period from 4/2/2003 through 7/01/2003. As indicated in the list of accomplishments below we have completed some long-term model scale bioreactor tests and are prepared to begin pilot scale bioreactor testing. Specific results and accomplishments for the second quarter of 2003 include: (1) Bioreactor support systems and test facilities: (a) Qualitative long-term survivability tests for S.C.1.2(2) on Omnisil have been successfully completed and results demonstrate a growth rate that appears to be acceptable. (b) Quantitative tests of long-term growth productivity for S.C.1.2(2) on Omnisil have been completed and initial results are promising. Initial results show that the mass of organisms doubled (from 54.9 grams to 109.8 grams) in about 5 weeks. Full results will be available as soon as all membranes and filters are completely dried. The growth rate should increase significantly with the initiation of weekly harvesting during the long term tests. (c) The phase 1 construction of the pilot scale bioreactor has been completed, including the solar collector and light distribution system. We are now in the phase of system improvement as we wait for CRF-2 results in order to be able to finalize the design and construction of the pilot scale system. (d) A mass transfer experimental setup was constructed in order to measure the mass transfer rate from the gas to the liquid film flowing over a membrane and to study the hydrodynamics of the liquid film flowing over a membrane in the bioreactor. Results were reported for mass transfer coefficient, film thickness, and fluid velocity over an Omnisil membrane with a ''drilled hole'' header pipe design. (2) Organisms and Growth Surfaces: (a) A selectivity approach was used to obtain a cyanobacterial culture with elevated resistance to acid pH. ...
Date: July 22, 2003
Creator: Kremer, Gregory; Bayless, David J.; Vis, Morgan; Prudich, Michael; Cooksey, Keith & Muhs, Jeff
Partner: UNT Libraries Government Documents Department

Enhanced Practical Photosynthetic CO2 Mitigation

Description: This report highlights significant achievements in the Enhanced Practical Photosynthetic CO{sub 2} Mitigation Project for the period ending 06/30/2004. The major accomplishment was the modification of the header and harvesting work, with a system designed to distribute algae at startup, sustain operations and harvest in one unit.
Date: July 15, 2004
Creator: Kremer, Gregory; Bayless, David J.; Vis, Morgan; Prudich, Michael; Cooksey, Keith & Muhs, Jeff
Partner: UNT Libraries Government Documents Department

Enhanced Practical Photosynthetic CO2 Mitigation

Description: This report highlights significant achievements in the Enhanced Practical Photosynthetic CO{sub 2} Mitigation Project during the ending 12/31/2004. Specific results and accomplishments for the program include review of pilot scale testing and design of a new bioreactor. Testing confirmed that algae can be grown in a sustainable fashion in the pilot bioreactor, even with intermittent availability of sunlight. The pilot-scale tests indicated that algal growth rate followed photon delivery during productivity testing.
Date: January 13, 2005
Creator: Kremer, Gregory; Bayless, David J.; Vis, Morgan; Prudich, Michael; Cooksey, Keith & Muhs, Jeff
Partner: UNT Libraries Government Documents Department

Enhanced Practical Photosynthetic CO2 Mitigation

Description: This final report highlights significant achievements in the Enhanced Practical Photosynthetic CO{sub 2} Mitigation Project during the period from 10/1/2001 through 01/02/2006. As indicated in the list of accomplishments below, our efforts during this project were focused on the selection of candidate organisms and growth surfaces and initiating long-term tests in the bench-scale and pilot-scale bioreactor test systems. Specific results and accomplishments for the program include: (1) CRF-2 test system: (a) Sampling test results have shown that the initial mass of algae loaded into the Carbon Recycling Facility Version 2 (CRF-2) system can be estimated with about 3% uncertainty using a statistical sampling procedure. (b) The pressure shim header pipe insert design was shown to have better flow for harvesting than the drilled-hole design. (c) The CRF-2 test system has undergone major improvements to produce the high flow rates needed for harvesting (as determined by previous experiments). The main changes to the system are new stainless steel header/frame units, with increased flow capacity and a modified pipe-end-sealing method to improve flow uniformity, and installation and plumbing for a new high flow harvesting pump. Qualitative system tests showed that the harvesting system performed wonderfully, cleaning the growth surfaces within a matter of seconds. (d) Qualitative tests have shown that organisms can be repopulated on a harvested section of a bioreactor screen, demonstrating that continuous bioreactor operation is feasible, with continuous cycles of harvesting and repopulating screens. (e) Final preparations are underway for quantitative, long-term tests in the CRF-2 with weekly harvesting. (2) Pilot-scale test system: (a) The construction of the pilot-scale bioreactor was completed, including the solar collector and light distribution system. Over the course of the project, the solar collector used in the light delivery system showed some degradation, but performed well overall. (b) Testing confirmed that algae can be ...
Date: January 15, 2006
Creator: Kremer, Gregory; Bayless, David J.; Vis, Morgan; Prudich, Michael; Cooksey, Keith & Muhs, Jeff
Partner: UNT Libraries Government Documents Department

Enhanced Practical Photosynthetic CO2 Mitigation

Description: This quarterly report documents significant achievements in the Enhanced Practical Photosynthetic CO{sub 2} Mitigation project during the period from 7/2/2003 through 10/01/2003. As indicated in the list of accomplishments below we are preparing for the final tests necessary to meet our project goals. Specific results and accomplishments for the third quarter of 2003 include: (1) Bioreactor support systems and test facilities: (A) The solar collector used in the light delivery system showed signs of degradation and hence had to be replaced by ORNL. A set of light readings were taken after the new solar collector was installed. The readings showed an acceptable light profile. (B) The CRF-2 test system has undergone major improvements to produce the high flow rates needed for harvesting (as determined by previous experiments). The main changes to the system are new stainless steel header/frame units with increased flow capacity and a modified pipe end sealing method to improve flow uniformity, and installation and plumbing for a new high flow harvesting pump. The improvements have been completed and the system is ready for testing. (C) The pilot scale bioreactor is ready for testing pending some information from the CRF-2 tests. (2) Organisms and Growth Surfaces: (A) The shape of the Chlorogloeopsis sp. cells (cyanobacteria) was found to be affected by environmental pH, which may be useful in culture quality control. Besides, the further investigation of this phenomenon suggested that the rate of cell adhesion to glass surface decreases upon medium alkalinization. Thus, harvesting effectiveness may be improved by increasing medium pH up to 9 before harvesting of cyanobacteria from a substratum.
Date: October 15, 2003
Creator: Kremer, Gregory; Bayless, David J.; Vis, Morgan; Prudich, Michael; Cooksey, Keith & Muhs, Jeff
Partner: UNT Libraries Government Documents Department