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N-Acylethanolamines and Plant Phospholipase D

Description: Recently, three distinct isoforms of phospholipase D (PLD) were identified in Arabidopsis thaliana. PLD α represents the well-known form found in plants, while PLD β and γ have been only recently discovered (Pappan et al., 1997b; Qin et al., 1997). These isoforms differ in substrate selectivity and cofactors required for activity. Here, I report that PLD β and γ isoforms were active toward N-acylphosphatidylethanolamine (NAPE), but PLD α was not. The ability of PLD β and γ to hydrolyze NAPE mar… more
Date: December 1998
Creator: Brown, Shea Austin
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Light Spectra Distributions in Temperate Conifer-Forest Canopy Gaps, Oregon and in Tropical Cloud-Forest Canopy, Venezuela

Description: Light spectra distributions were measured in two different montane forests: temperate and tropical. Spectral light measurements were made in different sized canopy gaps in the conifer forest at H. J. Andrews Experimental Forest in Oregon, USA. Researchers at Oregon State University created these gaps of 20 m, 30 m, and 50 m in diameter. In the tropical cloud forest, spectral light measurements were made in two plots that were permanently established at La Mucuy Parque Nacional in Venezuela, in … more
Date: December 1997
Creator: Monteleone, Susan Elaine
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Responses of Cultured Neuronal Networks to the Cannabinoid Mimetic Anandamide

Description: The effects of cannabinoid agonists on spontaneous neuronal network activity were characterized in murine spinal cord and auditory cortical cultures with multichannel extracellular recording using photoetched electrode arrays. Different cultures responded reproducibly with global decreases of spiking and bursting to anandamide and methanandamide, but each agonist showed unique minor effects on network activity. The two tissues responded in a tissue-specific manner. Spontaneous activity in spina… more
Date: May 1998
Creator: Morefield, Samantha I. (Samantha Irene)
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Cloning of Carbonic Anhydrase from Cotton (Gossypium hirsutum L.)

Description: Carbonic anhydrase is a ubiquitous zinc-metalloenzyme that catalyzes the interconversion of carbon dioxide and carbonate and has been found to play a wide range of roles in animals, plants and bacteria. Cotton genomic and cDNA libraries were screened for the plastidial isoform of carbonic anhydrase. The nucleotide sequences of two 1.2 Kb partial cDNA clones were determined. These clones exhibit high homology to carbonic anhydrases from other dicot plants and possess all the expected peptide mot… more
Date: December 1998
Creator: Local, Andrea
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Role of α-Keto Acids In Cyanide Detoxification and Assimilation by Pseudomonas Bacteria

Description: Cyanide was rapidly removed when added to culture supernatants of seven different Pseudomonas. The ability to remove cyanide was correlated with the accumulation of α-keto acids (pyruvate and α-ketoglutarate). These compounds react with cyanide forming less toxic cyanohydrins, thus conferring a mechanism for bacterial cyanide tolerance. When added to growth media the α-keto acids were shown also to serve as effective cyanide antagonists. While all bacteria tested accumulated α-keto acids, only … more
Date: December 1998
Creator: Pan, Guangliang
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Dalbergia and Albizia: Plantlet Production via Tissue Culture, Karyological Evaluation, and Seed Anatomy with Scanning Electron Microscopy

Description: A publication by the National Academy of Sciences, USA (1979) outlined some of the research need for a great variety of economically important woody species whose remaining genetic resources need urgently to be collected and conserved. A viable regeneration system was established via tissue and cell suspension culture for Albizia falcataria and A. lebbeck, two important wood yielding leguminous tree species. The culture medium was standardized after several trials to obtain callus from the leaf… more
Date: December 1998
Creator: Ghosh, Nabarun
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Regulation of Escherichia coli pyrBI Gene Expression in Pseudomonas fluorescens

Description: Pseudomonas fluorescens does not appear to regulate the enzymes of de novo pyrimidine biosynthesis at the level of gene expression. Little or no apparent repression of pyr gene expression is observed upon addition of exogenous pyrimidines to the growth medium. The Escherichia coli pyrBI genes for aspartate transcarbamoylase (ATCase) were sized down and cloned into the broad host range plasmid, pKT230. Upon introduction into a P.fluorescenspyrB mutant strain, ATCase showed repression in response… more
Date: May 1995
Creator: Shen, Weiping
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Cottonseed Microsomal N-Acylphosphatidylethanolamine Synthase: Identification, Purification and Biochemical Characterization of a Unique Acyltransferase

Description: N-Acylphosphatidylethanoiamine (NAPE) is synthesized in the microsomes of cotton seedlings by a mechanism that is possibly unique to plants, the ATP-, Ca2+-, and CoA-independent acylation ofphosphatidylethanolamine (PE) with unesterified free fatty acids (FFAs), catalyzed by NAPE synthase. A photoreactive free fatty acid analogue, 12-[(4- azidosalicyl)amino]dodecanoic acid (ASD), and its 125I-labeled derivative acted as substrates for the NAPE synthase enzyme.
Date: December 1998
Creator: McAndrew, Rosemary S. (Rosemary Smith)
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Subcellular Localization of N-acylphosphatidyl-ethanolamine Synthase in Cotyledons of Cotton Seedlings

Description: N-acylation of phosphatidylethanolamine (PE) with free fatty acids catalyzed by N-acyl phosphatidylethanolamine (NAPE) synthase was reported in cotyledons of 24-h-old cotton seedlings. Here I report subcellular localization of this enzyme. Differential centrifugation, sucrose density gradient fractionation,aqueous two-phase partitioning and electron microscopy techniques were utilized to elucidate subcellular site(s) of NAPE synthase. Marker enzymes were used to locate organelles in subcellular… more
Date: December 1995
Creator: Sriparameswaran, Anuja
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Luminescence Resonance Energy Transfer-Based Modeling of Troponin in the Presence of Myosin and Troponin/Tropomyosin Defining Myosin Binding Target Zones in the Reconstituted Thin Filament

Description: Mechanistic details on the regulation of striated muscle contraction still need to be determined, particularly the specific structural locations of the elements comprising the thick and thin filaments. Of special interest is the location of the regulatory component, troponin, on the actin filament and how its presence influences the behavior of myosin binding to the thin filament. In the present study: (1) Luminescence resonance energy transfer was used to monitor potential conformational chang… more
Date: May 2009
Creator: Patel, Dipesh A.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Analyzing Mass Spectrometry Imaging Data of 13C-Labeled Phospholipids in Camelina sativa and Thlaspi arvense (Pennycress) Embryos

Description: This article uses currently available software and techniques to describe a workflow to analyze ¹³C-labeled isotopologues of the membrane lipid and storage oil lipid intermediate—phosphatidylcholine (PC). Results demonstrated greater ¹³C-isotopic labeling in the cotyledons of developing embryos compared with the embryonic axis.
Date: March 4, 2021
Creator: Romsdahl, Trevor B.; Kambhampati, Shrikaar; Koley, Somnath; Yadav, Umesh P.; Alonso, Ana Paula; Allen, Doug K. et al.
Item Type: Refine your search to only Article
Partner: UNT College of Science
open access

Malonylation of Glucosylated N-Lauroylethanolamine: A New Pathway That Determines N-Acylethanolamine Metabolic Fate in Plants

Description: Article studying Malonylation of Glucosylated N-Lauroylethanolamine. Results indicate that glucosylation of NAE 12:0 by a yet to be determined glucosyltransferase and its subsequent malonylation by PMAT1 could represent a mechanism for modulating the biological activities of NAEs in plants.
Date: December 30, 2016
Creator: Khan, Bibi Rafeiza; Wherritt, Daniel J.; Huhman, David; Sumner, Lloyd W.; Chapman, Kent Dean & Blancaflor, Elison B.
Item Type: Refine your search to only Article
Partner: UNT College of Science
open access

Structural analysis of a plant fatty acid amide hydrolase provides insights into the evolutionary diversity of bioactive acylethanolamides

Description: Article reporting the three-dimensional crystal structures of the signal-terminating enzyme fatty acid amide hydrolase (FAAH) from Arabidopsis in its apo and ligand-bound forms at 2.1- and 3.2-Å resolutions, respectively.
Date: March 20, 2019
Creator: Aziz, Mina; Wang, Xiaoqiang; Tripathi, Ashutosh; Bankaitis, Vytas A. & Chapman, Kent Dean
Item Type: Refine your search to only Article
Partner: UNT College of Science
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Temperature-sensitive Post-translational Regulation of Plant Omega-3 Fatty-acid Desaturases Is Mediated by the Endoplasmic Reticulum-associated Degradation Pathway

Description: Article expressing two closely related plant FAD3 genes in yeast cells and found that their enzymes produced significantly different amounts of omega-3 fatty acids and that these differences correlated to differences in rates of protein turnover. The findings indicate that Fad3 protein abundance is regulated by a combination of cis-acting degradation signals and the ubiquitin-proteasome pathway and that modulation of Fad3 protein amounts in response to temperature may represent one mechanism of… more
Date: July 9, 2010
Creator: O'Quin, Jami B.; Bourassa, Linda; Zhang, Daiyuan; Shockey, Jay M.; Gidda, Satinder K.; Fosnot, Spencer et al.
Item Type: Refine your search to only Article
Partner: UNT College of Science
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Lipoxygenase-mediated Oxidation of Polyunsaturated N-Acylethanolamines in Arabidopsis

Description: Article hypothesizing that lipoxygenase (LOX) participated in the metabolism of PU-NAEs through the formation of NAE-oxylipins. The data suggests that Arabidopsis LOXs indeed compete with FAAH to metabolize PU-NAEs during seedling establishment. Identification of endogenous amide-conjugated oxylipins suggests potential significance of these metabolites in vivo, and FAAH mutants may offer opportunities to address this in the future.
Date: April 29, 2011
Creator: Kilaru, Aruna; Herrfurth, Cornelia; Keereetaweep, Jantana; Hornung, Ellen; Venables, Barney J.; Feussner, Ivo et al.
Item Type: Refine your search to only Article
Partner: UNT College of Science
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Visualization of Lipid Droplet Composition by Direct Organelle Mass Spectrometry

Description: Article reporting an approach that combines the visualization of individual lipid droplets (LDs), microphase extraction of lipid components from droplets, and the direct identification of lipid composition by nanospray mass spectrometry, even to the level of a single LD. The triacylglycerol (TAG) composition of LDs from several plant sources (mature cotton (Gossypium hirsutum) embryos, roots of cotton seedlings, and Arabidopsis thaliana seeds and leaves) were examined by direct organelle mass s… more
Date: February 4, 2011
Creator: Horn, Patrick J.; Ledbetter, Nicole R.; James, Christopher N.; Hoffman, William D.; Case, Charlene R.; Verbeck, Guido F. et al.
Item Type: Refine your search to only Article
Partner: UNT College of Science
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Heterogeneous Distribution of Erucic Acid in Brassica napus Seeds

Description: Article describes study examining low- and high-erucic acid accessions of B. napus seeds for the distribution of erucic acid-containing lipids and the gene transcripts encoding the enzymes involved in pathways for its incorporation into triacylglycerols (TAGs) across the major tissues of the seeds.
Date: January 29, 2020
Creator: Lu, Shaoping; Aziz, Mina; Sturtevant, Drew; Chapman, Kent Dean & Guo, Liang
Item Type: Refine your search to only Article
Partner: UNT College of Science
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Effects of cultivar and nitrogen application rate on lint, seed, oil, and protein yields of field-grown cotton

Description: Article describes how the objective of this study was to evaluate the lint, seed, oil, and protein yield responses of cultivars with different seed mass and composition to N application rates (0–168 kg N ha−1) for field-grown cotton. The authors conclude that variability in the distribution of photosynthates to fiber and seed as well as seed oil and protein composition can significantly alter trends in fiber, seed, and seed component yields in response to cultivar or N application rates for fie… more
Date: March 9, 2023
Creator: Parkash, Ved; Snider, John L.; Bruce, Aaron; Ermanis, Alessandro; Virk, Gurpreet; Kaur, Navneet et al.
Item Type: Refine your search to only Article
Partner: UNT College of Science
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Quantification of Botrytis cinerea Growth in Arabidopsis thaliana

Description: Article describes how yield losses attributed to plant pathogens pose a serious threat to plant productivity and food security. Here, the authors describe two methods to quantify B. cinerea development in Arabidopsis thaliana genotypes through measurements of lesion development and quantification of fungal genomic DNA in infected tissues.
Date: August 20, 2023
Creator: Scholz, Patricia; Chapman, Kent Dean; Ischebeck, Till & Guzha, Athanas
Item Type: Refine your search to only Article
Partner: UNT College of Science
open access

Analysis of Pectin-derived Monosaccharides from Arabidopsis Using GC–MS

Description: Article describes how pectin is a complex polysaccharide present in the plant cell wall, whose composition is constantly remodeled to adapt to environmental or developmental changes. Here, authors describe a quick and efficient gas chromatography–mass spectrometry (GC–MS)-based method that allows the differential analysis of pectin monosaccharide composition in plants under different conditions or between mutant plants and their respective wild types.
Date: August 20, 2023
Creator: Scholz, Patricia; Chapman, Kent Dean; Ischebeck, Till & Guzha, Athanas
Item Type: Refine your search to only Article
Partner: UNT College of Science
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Better together: Protein partnerships for lineage-specific oil accumulation

Description: Article reviews recent evidence that protein–protein interactions in each of the three major phases of oil formation appear to have profound effects on specialized oil accumulation. This article is part of the special issue: Physiology and metabolism (2022).
Date: February 24, 2022
Creator: Busta, Lucas; Chapman, Kent Dean & Cahoon, Edgar B.
Item Type: Refine your search to only Article
Partner: UNT College of Science
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N-acylethanolamide metabolizing enzymes are upregulated in human neural progenitor-derived neurons exposed to sub-lethal oxidative stress

Description: Article is a study determining whether ReN cells, a superior cell model system for studying neurodevelopment, differentiation, and neuroprotection, express proteins involved in canonical eCB NAE signaling and whether oxidative stress can induce their expression. This study is a first step toward determining how oxidative stress affects CB1, CB2, FAAH, NAAA, and NAPE-PLD expression and their potential defense against oxidative stress.
Date: August 8, 2022
Creator: Duncan, Scott R.; Riordan, Sean M.; Hall, Conner W.; Payne, Andrew J.; Chapman, Kent Dean & Koulen, Peter
Item Type: Refine your search to only Article
Partner: UNT College of Science
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