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Synthesis and Metabolism of Carbonyl-C14 Pyruvic andHydroxypyruvic Acids in Algae

Description: 1. Pyruvic and hydroxypyruvic acids a r e metabolized by Scenedesmus. 2. The products of metabolism of pyruvic -2 -C{sup 14} and hydroxypyruvic-2 -C{sup 14} acids a r e essentially identical to those of C{sup 14}-O fixations. 3. Lipids a r e rapidly formed i n the light from both substrates. In the dark the major products a r e intermediates of the tricarboxylic acid cycle. 4. Zt does not appear likely that f r e e hydroxypyruvic acid is a photosynthetic intermediate, 5 . Tricarboxylic acid cycle intermediates a r e formed from exogenous pyruvate a s fast in the light a s in the dark.
Date: March 30, 1955
Creator: Milhaud, Gerhard; Benson, Andrew A. & Calvin, M.
Partner: UNT Libraries Government Documents Department

The Path of Carbon in Photosynthesis VIII. The Role of MalicAcid

Description: Malonate has been found to inhibit the formation of malic acid during short periods of photosynthesis with radioactive carbon dioxide. This result, together with studies which show the photosynthetic cycle to be operating normally at the same time, indicates that malic acid is not an intermediate in photosynthesis but is probably closely related to some intermediate of the cycle. Absence of labeled succinic and fumaric acids in these experiments, in addition to the failure of malonate to inhibit photosynthesis, precludes the participation of these acids as intermediates in photosynthesis.
Date: January 25, 1950
Creator: Bassham, James A.; Benson, Andrew A. & Calvin, Melvin
Partner: UNT Libraries Government Documents Department

S-Acyl Thioctic Acid Derivatives in Aerobacter Aerogenes andScenedesmus

Description: 1. Acetyl thioctic acid has been prepared chemically and i t s chromatographic and acetylating behavior i s described. 2. A C{sup 14} -containing substance has been found in Scenedesmus, photosynthesizing in the presence of a-C{sup 14}-pyruvate, which has properties suggesting that it i s acetyl thioctic acid. 3 . A C{sup 14}-containing substance has been found in Aerobacter aerogenes, metabolizing a-C{sup 14}-pyruvate, which shows the properties of a labile conjugate of thioctic acid with some relatively polar groups. 4. Acetyl thioctic acid i s formed in vitro when light acts on a solution of thioctic acid and pyruvate.
Date: March 30, 1955
Creator: Milhaud, Gerard; Benson, Andrew A.; Fuller, R. Clinton; Milhaud,Vera & Calvin, M.
Partner: UNT Libraries Government Documents Department