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Dielectrophoretic manipulation of particles for use in microfluidic devices

Description: Amplification and hybridization of DNA are commonly used techniques to verify the presence of a specific DNA sequence in a test sample. Automatic sample handling to concentrate and purify sample prior to amplification is desirable both from the cost standpoint and from the standpoint of reducing the possibility of sample contamination. This paper explores the use of the dielectrophoretic force to manipulate DNA, Bacillus globigii spores, and Erwinia herbicola bacteria to provide concentration a… more
Date: June 23, 1999
Creator: Belgrader, P.; Bettencourt, K.; Hamilton, J.; Miles, R. & Nasarabadi, S.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Manipulation of DNA for use in microfluidic devices

Description: MEMS microfluidic systems are becoming increasingly popular as a way to integrate sample preparation and biological assays on a single substrate. The resulting reduction in manual operations and reduced reagent use can lead to significant cost savings in performing biological tests. The authors have explored the use of small scale dielectrophoresis and electrophoresis as a way to manipulate DNA for sample preparation in DNA-based assays. The use of electric fields to manipulate DNA is readily a… more
Date: November 18, 1998
Creator: Belgrader, P.; Bettencourt, K.; Davidson, J. C.; Mariella, R. P.; Miles, R.; Nasarabadi, N. S. et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Final Report BW Sample Collection& Preparation Device

Description: The objective of this project was to develop the technique needed to prepare a field collected sample for laboratory analysis and build a portable integrated biological detection instrument with new miniaturized and automated sample purification capabilities. The device will prepare bacterial spores, bacterial vegetative cells, and viral particles for PCR amplification.
Date: January 31, 2002
Creator: Koopman, R P; Belgrader, P; Meyer, G; Benett, W J; Richards, J B; Hadley, D R et al.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Autonomous system for pathogen detection and identification

Description: This purpose of this project is to build a prototype instrument that will, running unattended, detect, identify, and quantify BW agents. In order to accomplish this, we have chosen to start with the world� s leading, proven, assays for pathogens: surface-molecular recognition assays, such as antibody-based assays, implemented on a high-performance, identification (ID)-capable flow cytometer, and the polymerase chain reaction (PCR) for nucleic-acid based assays. With these assays, we must integr… more
Date: September 24, 1998
Creator: Belgrader, P.; Benett, W.; Langlois, R.; Long, G.; Mariella, R.; Milanovich, F. et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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