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Analysis of the Expression Profiles of Two Isoforms of the Antifungal Protein Osmotin from Gossypium hirsutum

Description: The expression of two cotton osmotin genes was evaluated in terms of the mRNA and protein expression patterns in response to chemical inducers such as ethylene, hydrogen peroxide, and sodium chloride. Reverse transcriptase-polymerase chain reactions (RT-PCR) indicated that osmotin mRNAs are expressed constitutively in root tissues of cotton plants, and that they are rapidly induced in leaf and stem tissues upon ethylene treatment. Real time RT-PCR indicated that osmotin transcript levels were… more
Date: May 2007
Creator: Spradling, Kimberly Diane
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Establishing genetic and physiological baselines for the black-tailed prairie dog (Cynomys ludovicianus).

Description: The black-tailed prairie dog (Cynomys ludovicianus) has experienced dramatic declines over much of its historical range due to habitat loss, plague, poisonings, and shootings. Many populations now occur as isolated genetic relicts. A multiple locus genetic profile was obtained using microsatellite analyses of six polymorphic nucleotide repeats from 319 black-tailed prairie dogs collected from 16 colonies throughout the state of Texas. This assessment revealed that existing populations have suff… more
Date: May 2007
Creator: Biggs, Cindy Dawn
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

DHS Student Report

Description: Throughout this project I have been involved in every step of the protocol. After proper training, I was introduced to the necessary lab techniques for the project. From then on it has been my responsibility to perform the necessary tasks to identify and isolate the mutants. This includes carrying out a detailed protocol of mixing reagents, streaking and incubating plates, inoculating cultures and evaluating any results in order to guide my actions for the next antibiotic concentration level. S… more
Date: July 23, 2007
Creator: Wynne, Elizabeth K.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Disruption of Maternal DNA Repair Increases Sperm-DerivedChromosomal Aberrations

Description: The final weeks of male germ cell differentiation occur in aDNA repair-deficient environment and normal development depends on theability of the egg to repair DNA damage in the fertilizing sperm. Geneticdisruption of maternal DNA double-strand break repair pathways in micesignificantly increased the frequency of zygotes with chromosomalstructural aberrations after paternal exposure to ionizing radiation.These findings demonstrate that radiation-induced DNA sperm lesions arerepaired after fertil… more
Date: February 7, 2007
Creator: Marchetti, Francesco; Essers, Jeroun; Kanaar, Roland & Wyrobek,Andrew J.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Task Group 7B: Cellular and Molecular Mechanisms of Biological Aging: The Roles of Nature, Nurture and Chance in the Maintenance of Human Healthspan

Description: The degree to which an individual organism maintains healthspan and lifespan is a function of complex interactions between genetic inheritance ('nature'), environment, including cultural inheritance (nurture) and stochastic events ('luck' or 'chance'). This task group will focus upon the role of chance because it is so poorly understood and because it appears to be of major importance in the determination of individual variations in healthspan and lifespan within species. The major factor deter… more
Date: November 14, 2007
Creator: Weier, Heinz-Ulrich; Arya, Suresh; Grant, Christine; Miller, Linda; Ono, Santa Jeremy; Patil, Chris et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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Patterns and Implications of Gene Gain and Loss in the Evolution of Prochlorococcus

Description: Prochlorococcus is a marine cyanobacterium that numerically dominates the mid-latitude oceans and is the smallest known oxygenic phototroph. Numerous isolatesfrom diverse areas of the world's oceans have been studied and shown to be physiologically and genetically distinct. All isolates described thus far can be assigned to either a tightly clustered high-light (HL)-adapted clade, or a more divergent low-light (LL)-adapted group. The 16S rRNA sequences of the entire Prochlorococcus group differ… more
Date: July 30, 2007
Creator: Lapidus, Alla; Kettler, Gregory C.; Martiny, Adam C.; Huang, Katherine; Zucker, Jeremy; Coleman, Maureen L. et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

National Childhood Cancer Foundation

Description: The initiative will enable the COG Biopathology Center (Biospecimen Repository), the Molecular Genetics Laboratory and other participating reference laboratories to upload large data sets to the eRDES. The capability streamlines data currency and accuracy allowing the centers to export data from local systems and import the defined data to the eRDES. The process will aid in the best practices which have been defined by the Office of Biorepository and Biospecimen Research (OBBR) and the Group Ba… more
Date: November 14, 2007
Creator: Reaman, Gregory
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Bio-Engineering High Performance Microbial Strains for MEOR

Description: The main objectives of this three-year research project are: (1) to employ the latest advances in genetics and bioengineering, especially Directed Protein Evolution technology, to improve the effectiveness of the microbial enhanced oil recovery (MEOR) process. (2) to improve the surfactant activity and the thermal stability of bio-surfactant systems for MEOR; and (3) to develop improved laboratory methods and tools that screen quickly candidate bio-systems for EOR. Biosurfactants have been rece… more
Date: December 30, 2007
Creator: Fang, Xiangdong; Wang, Qinghong & Shuler, Patrick
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Uncharted Microbial World: Microbes and Their Activities in the Environment

Description: Microbes are the foundation for all of life. From the air we breathe to the soil we rely on for farming to the water we drink, everything humans need to survive is intimately coupled with the activities of microbes. Major advances have been made in the understanding of disease and the use of microorganisms in the industrial production of drugs, food products and wastewater treatment. However, our understanding of many complicated microbial environments (the gut and teeth), soil fertility, and b… more
Date: December 31, 2007
Creator: Harwood, Caroline & Buckley, Merry.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

The Genome of Methylobacillus Flagellatus, the Molecular Basis for Obligate Methylotrophy, and the Polyphyletic Origin of Methylotrophy

Description: Along with methane, methanol and methylated amines representimportant biogenic atmospheric constituents, thus not only methanotrophs,but also non-methanotrophic methylotrophs play a significant role inglobal carbon cycling. The complete genome of a model obligate methanoland methylamine utilizer, Methylobacillus flagellatus (strain KT) wassequenced. The genome is represented by a single circular chromosome ofapproximately 3 Mb pairs, potentially encoding a total of 2,766 proteins.Based on genom… more
Date: January 8, 2007
Creator: Chistoserdova, Ludmila; Lapidus, Alla; Han, Cliff; Goodwin, Lynne; Saunders, Liz; Brettin, Tom et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Mathematically Reduced Chemical Reaction Mechanism Using Neural Networks

Description: This is the final technical report for the project titled 'Mathematically Reduced Chemical Reaction Mechanism Using Neural Networks'. The aim of the project was to develop an efficient chemistry model for combustion simulations. The reduced chemistry model was developed mathematically without the need of having extensive knowledge of the chemistry involved. To aid in the development of the model, Neural Networks (NN) was used via a new network topology known as Non-linear Principal Components A… more
Date: August 31, 2007
Creator: Huque, Ziaul
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Genome-wide experimental determination of barriers to horizontal gene transfer

Description: Horizontal gene transfer, in which genetic material is transferred from the genome of one organism to another, has been investigated in microbial species mainly through computational sequence analyses. To address the lack of experimental data, we studied the attempted movement of 246,045 genes from 79 prokaryotic genomes into E. coli and identified genes that consistently fail to transfer. We studied the mechanisms underlying transfer inhibition by placing coding regions from different species … more
Date: September 24, 2007
Creator: Rubin, Edward; Sorek, Rotem; Zhu, Yiwen; Creevey, Christopher J.; Francino, M. Pilar; Bork, Peer et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Improved Soybean Oil for Biodiesel Fuel

Description: The goal of this program was to generate information on the utility of soybean germplasm that produces oil, high in oleic acid and low in saturated fatty acids, for its use as a biodiesel. Moreover, data was ascertained on the quality of the derived soybean meal (protein component), and the agronomic performance of this novel soybean germplasm. Gathering data on these later two areas is critical, with respect to the first, soybean meal (protein) component is a major driver for commodity soybean… more
Date: November 30, 2007
Creator: Clemente, Tom & Gerpen, Jon Van
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Regulation of In Situ to Invasive Breast Carcinoma Transition

Description: The transition of ductal carcinoma in situ (DCIS) to invasive carcinoma is a key event in breast tumor progression that is poorly understood. Comparative molecular analysis of tumor epithelial cells from in situ and invasive tumors has failed to identify consistent tumor stage-specific differences. However, the myoepithelial cell layer, present only in DCIS, is a key distinguishing and diagnostic feature. To determine the contribution of non-epithelial cells to tumor progression, we analyzed th… more
Date: March 13, 2007
Creator: Hu, Min; Carroll, Danielle K.; Weremowicz, Stanislawa; Chen, Haiyan; Carrasco, Daniel; Richardson, Andrea et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Drosophila by the dozen

Description: This year's conference on Drosophila research illustratedwell the current focus of Drosophila genomics on the comprehensiveidentification of functional elements in the genome sequence, includingmRNA transcripts arising from multiple alternative start sites and splicesites, a multiplicity of noncoding transcripts and small RNAs,identification of binding sites for transcription factors, sequenceconservation in related species and sequence variation within species.Resources and technologies for ge… more
Date: July 13, 2007
Creator: Celniker, Susan E. & Hoskins, Roger A.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

DNA Repair Decline During Mouse Spermiogenesis Results in the Accumulation of Heritable DNA Damage

Description: The post-meiotic phase of mouse spermatogenesis (spermiogenesis) is very sensitive to the genomic effects of environmental mutagens because as male germ cells form mature sperm they progressively lose the ability to repair DNA damage. We hypothesized that repeated exposures to mutagens during this repair-deficient phase result in the accumulation of heritable genomic damage in mouse sperm that leads to chromosomal aberrations in zygotes after fertilization. We used a combination of single or fr… more
Date: December 1, 2007
Creator: Marchetti, Francesco; Marchetti, Francesco & Wyrobek, Andrew J.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Ovarian carcinomas with genetic and epigenetic BRCA1 loss havedistinct molecular abnormalities

Description: Subclassification of ovarian carcinomas can be used to guide treatment and determine prognosis. Germline and somatic mutations, loss of heterozygosity (LOH), and epigenetic events such as promoter hypermethylation can lead to decreased expression of BRCA1/2 in ovarian cancers. The mechanism of BRCA1/2 loss is a potential method of subclassifying high grade serous carcinomas. A consecutive series of 49 ovarian cancers was assessed for mutations status of BRCA1 and BRCA2, LOH at the BRCA1 and BRC… more
Date: July 23, 2007
Creator: Press, Joshua Z.; De Luca, Alessandro; Boyd, Niki; Young, Sean; Troussard, Armelle; Ridge, Yolanda et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Advances in Genome Biology & Technology

Description: This year's meeting focused on the latest advances in new DNA sequencing technologies and the applications of genomics to disease areas in biology and biomedicine. Daytime plenary sessions highlighted cutting-edge research in areas such as complex genetic diseases, comparative genomics, medical sequencing, massively parallel DNA sequencing, and synthetic biology. Technical approaches being developed and utilized in contemporary genomics research were presented during evening concurrent sessions… more
Date: December 1, 2007
Creator: Thomas J. Albert, Jon R. Armstrong, Raymond K. Auerback, W. Brad Barbazuk, et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Dissecting Biological Dark Matter: Single Cell Genetic Analysis of TM7, a Rare and Uncultivated Microbe from the Human Mouth

Description: We have developed a microfluidic device that allows the isolation and genome amplification of individual microbial cells, thereby enabling organism-level genomic analysis of complex microbial ecosystems without the need for culture. This device was used to perform a directed survey of the human subgingival crevice and to isolate bacteria having rod-like morphology. Several isolated microbes had a 16S rRNA sequence that placed them in candidate phylum TM7, which has no cultivated or sequenced me… more
Date: July 1, 2007
Creator: Fenner, Marsha W; Marcy, Yann; Ouverney, Cleber; Bik, Elisabeth M.; Losekann, Tina; Ivanova, Natalia et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Kootenai River White Sturgeon Recovery Implementation Plan and Schedule; 2005-2010, Technical Report 2004-2005.

Description: Kootenai River white sturgeon have been declining for at least 50 years and extinction of the wild population is now imminent (Paragamian et al. 2005). Only 630 adults were estimated to remain in 2002 from a population ten times that size just 20 years ago. Significant recruitment of young sturgeon has not been observed since the early 1970s and consistent annual recruitment has not been seen since the 1950s. The remaining wild population consists of a cohort of large, old fish that is declinin… more
Date: March 1, 2007
Creator: Anders, Paul
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Development and Characterization of A Multiplexed RT-PCR Species Specific Assay for Bovine and one for Porcine Foot-and-Mouth Disease Virus Rule-Out

Description: Lawrence Livermore National Laboratory (LLNL), in collaboration with the Department of Homeland Security (DHS) and the United States Department of Agriculture (USDA), Animal and Plant Health Inspection Services (APHIS) has developed candidate multiplexed assays that may potentially be used within the National Animal Health Laboratory Network (NAHLN), the National Veterinary Services Laboratory (Ames, Iowa) and the Plum Island Animal Disease Center (PIADC). This effort has the ability to improve… more
Date: August 6, 2007
Creator: Smith, Sally M; Danganan, Linda; Tammero, Lance; Vitalis, Beth; Lenhoff, Ray; Naraghi-arani, Pejman et al.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Bioforensics: Characterization of biological weapons agents by NanoSIMS

Description: The anthrax attacks of Fall 2001 highlight the need to develop forensic methods based on multiple identifiers to determine the origin of biological weapons agents. Genetic typing methods (i.e., DNA and RNA-based) provide one attribution technology, but genetic information alone is not usually sufficient to determine the provenance of the material. Non-genetic identifiers, including elemental and isotopic signatures, provide complementary information that can be used to identify the means, geogr… more
Date: February 26, 2007
Creator: Weber, P K; Ghosal, S; Leighton, T J; Wheeler, K E & Hutcheon, I D
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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