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Cloning of Carbonic Anhydrase from Cotton (Gossypium hirsutum L.)

Description: Carbonic anhydrase is a ubiquitous zinc-metalloenzyme that catalyzes the interconversion of carbon dioxide and carbonate and has been found to play a wide range of roles in animals, plants and bacteria. Cotton genomic and cDNA libraries were screened for the plastidial isoform of carbonic anhydrase. The nucleotide sequences of two 1.2 Kb partial cDNA clones were determined. These clones exhibit high homology to carbonic anhydrases from other dicot plants and possess all the expected peptide mot… more
Date: December 1998
Creator: Local, Andrea
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Dalbergia and Albizia: Plantlet Production via Tissue Culture, Karyological Evaluation, and Seed Anatomy with Scanning Electron Microscopy

Description: A publication by the National Academy of Sciences, USA (1979) outlined some of the research need for a great variety of economically important woody species whose remaining genetic resources need urgently to be collected and conserved. A viable regeneration system was established via tissue and cell suspension culture for Albizia falcataria and A. lebbeck, two important wood yielding leguminous tree species. The culture medium was standardized after several trials to obtain callus from the leaf… more
Date: December 1998
Creator: Ghosh, Nabarun
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Physical and Functional Characterization of the xy1XYZ Region From TOL Plasmid pDK1 and its Associated Downstream Regulatory Elements

Description: The nucleotide sequence for the pDKl TOL plasmid region encoding toluate-1,2-dioxygenase (Xy1XYZ, TO) was determined. TO is the first enzyme in the meta-cleavage operon, responsible for the conversion of toluates and benzoates to their carboxy-substituted diols. DNA sequence analysis revealed the presence of three open reading frames (ORF). The three ORFs correspond to xylX (1353 bp), xylY (486 bp) and xylZ (1008 bp), encoding predicted protein products of 51370 Da, 19368 Da and 36256 Da, respe… more
Date: August 1998
Creator: Hares, Douglas R. (Douglas Ryan)
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Design and construction of deinococcus radiodurans for biodegradation of organic toxins at radioactive DOE waste sites. 1998 annual progress report

Description: 'A 1992 survey of DOE waste sites indicates that about 32% of soils and 45% of groundwaters at these sites contain radionuclides and metals plus an organic toxin class. The most commonly reported combinations of these hazardous compounds being radionuclides and metals (e.g., U, Pu, Cs, Pb, Cr, As) plus chlorinated hydrocarbons (e.g., trichloroethylene), fuel hydrocarbons (e.g., toluene), or polychlorinated biphenyls (e.g., Arochlor 1248). These wastes are some of the most hazardous pollutants a… more
Date: June 1, 1998
Creator: Daly, M. J.; Wackett, L. P. & Minton, K. W.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Genetic analysis of stress responses in soil bacteria for enhanced bioremediation of mixed contaminants. 1998 annual progress report

Description: 'To realize the full potential of bioremediation, the individual bacterial responses to the stresses (lack of nutrients or oxygen; mixed pollutants) encountered at contaminated sites must be understood. This information can then be extrapolated to field applications using indigenous bacteria or genetically engineered micro-organisms. Studying bacterial response to stresses presents an opportunity for improving bioremediation strategies, both with indigenous populations and genetically engineere… more
Date: June 1, 1998
Creator: Wong, K. K.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Phytoremediation of ionic and methyl mercury pollution

Description: 'The long-term objective of the research is to manipulate single-gene traits into plants, enabling them to process heavy metals and remediate heavy-metal pollution by resistance, sequestration, removal, and management of these contaminants. The authors are focused on mercury pollution as a case study of this plant genetic engineering approach. The working hypothesis behind this proposal was that transgenic plants expressing both the bacterial organo mercury lyase (merB) and the mercuric ion red… more
Date: June 1, 1998
Creator: Meagher, R.B.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Monitoring genetic and metabolic potential for in situ bioremediation: Mass spectrometry. 1998 annual progress report

Description: 'A number of DOE sites are contaminated with dense non-aqueous phase liquids (DNAPLs) such as carbon tetrachloride and trichloroethylene. At many of these sites, microbial bioremediation is an attractive strategy for cleanup, since it has the potential to degrade DNAPLs in-situ. A rapid screening method to determine the broad range potential of a site''s microbial population for contaminant degradation would greatly facilitate assessment for in-situ bioremediation, as well as for monitoring ong… more
Date: January 1, 1998
Creator: Buchanan, M. V.; Hurst, G. B.; Doktycz, M. J.; Britt, P. F.; Weaver, K.; Lidstrom, M. et al.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Molecular genetics of metal detoxification: Prospects for phytoremediation. 1998 annual progress report

Description: 'The authors seek to define the genes involved in heavy metal tolerance and sequestration. Two complementary approaches were taken: (1) clone and characterize the genes that complement cadmium hypersensitive mutants of fission yeast, specifically those responsible for production of metal-binding complexes, and (2) isolate genes that can confer cadmium hypertolerance to wild type strains of fission yeast. This work summarizes present status of a 3 year project.'
Date: June 1, 1998
Creator: Ow, D.W.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Forensic DNA Extraction Strategies for PCR Analysis

Description: There is a transition nationwide on the analysis of forensic evidentiary stains containing biological material from traditional serology to Polymerase Chain Reaction (PCR) methodologies. The increased sensitivity of PCR, the limited number of alleles at each locus, and the necessity of producing unambiguous data for entry into the FBI's Combined DNA Index System make this study of extraction procedures of utmost importance. A "single tube" extraction procedure for blood stains collected onto FT… more
Date: May 1998
Creator: Van Winkle, Carolyn
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Nontraditional inheritance: Genetics and the nature of science, now titled, The puzzle of inheritance: Genetics and the methods of science. Final report

Description: This project led to the development of an instructional module designed for use in high school biology classes. The module contains two major components. The first is an overview for teachers, which introduces the module, describes the Human Genome Project, and addresses issues in the philosophy of science and some of the ethical, legal, and social implications of research in genetics. It provides a survey of fundamental genetics concepts and of new, nontraditional concepts of inheritance. The … more
Date: August 31, 1998
Creator: McInerney, J.D.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Automatic generation of warehouse mediators using an ontology engine

Description: The Data Foundry research project at LLNL is investigating data warehousing in highly dynamic scientific environments. Specifically, we are developing a data warehouse to aid structural biologists in genetics research. Upon completion, this warehouse will present a uniform view of data obtained from several heterogeneous data sources containing distinct but related data from various genetics domains. Our warehouse uses a mediated data warehouse architecture in which only some data is represente… more
Date: March 4, 1998
Creator: Critchlow, T.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Genetic secrets: Protecting privacy and confidentiality in the genetic era

Description: Few developments are likely to affect human beings more profoundly in the long run than the discoveries resulting from advances in modern genetics. Although the developments in genetic technology promise to provide many additional benefits, their application to genetic screening poses ethical, social, and legal questions, many of which are rooted in issues of privacy and confidentiality. The ethical, practical, and legal ramifications of these and related questions are explored in depth. The br… more
Date: July 1, 1998
Creator: Rothstein, M.A.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Genetics in methylotrophic bacteria: Appendix. Final report

Description: This research has focused primarily on promoters in Methylobacterium extorquens AM1 and in methanotrophic bacteria. In Methylobacterium extorquens work continued on the moxF promoter. The author constructed chromosomal lacZ fusions of this promoter to avoid the regulation problems of plasmid-borne fragments and has shown that this is regulated normally in the chromosome. She has constructed lacZ fusions to some of the mox genes involved in the synthesis of the cofactor, PQQ, in order to carry o… more
Date: September 1, 1998
Creator: Lidstrom, M.E.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

[Genetics in methylotrophic bacteria]. Final progress report, July 1, 1987--June 30, 1995

Description: This project is related to development of genetic techniques for methylotrophic bacteria and the use of these to study genes involved in methanol oxidation. During the first two years of the project the authors defined the genetic organization of these Mox (methanol oxidation) genes and started to define their regulatory regions. During the next three year period, work involved defining genetic organization of key methylotrophic genes, studying upstream regions involved in transcription, and de… more
Date: September 1, 1998
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

[Genetics in methylotrophic bacteria]. Progress report, July 1, 1992--June 30, 1995

Description: The focus of this project has been to identify and characterize mox genes and other methylotrophy genes in both methane- and methanol-utilizing bacteria, and study expression of those genes. In the last three years of support, the project has focused on identifying methylotrophy genes and the regions involved in their expression for comparative purposes, and has begun the process of analyzing the genes involved in transcriptional regulation of the mox system in the strain for which the authors … more
Date: September 1, 1998
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Genetic secrets: Protecting privacy and confidentiality in the genetic era. Final report

Description: Few developments are likely to affect human beings more profoundly in the long run than the discoveries resulting from advances in modern genetics. Although the developments in genetic technology promise to provide many additional benefits, their application to genetic screening poses ethical, social, and legal questions, many of which are rooted in issues of privacy and confidentiality. The ethical, practical, and legal ramifications of these and related questions are explored in depth. The br… more
Date: September 1, 1998
Creator: Rothstein, M.A.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Partnering for functional genomics research conference: Abstracts of poster presentations

Description: This reports contains abstracts of poster presentations presented at the Functional Genomics Research Conference held April 16--17, 1998 in Oak Ridge, Tennessee. Attention is focused on the following areas: mouse mutagenesis and genomics; phenotype screening; gene expression analysis; DNA analysis technology development; bioinformatics; comparative analyses of mouse, human, and yeast sequences; and pilot projects to evaluate methodologies.
Date: June 1, 1998
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

DataFoundry: Warehousing techniques for dynamic environments

Description: Data warehouses and data marts have been successfully applied to a multitude of commercial business applications as tools for integrating and providing access to data located across an enterprise. Although the need for this capability is as vital in the scientific world as in the business domain, working warehouses in our community are scarce. A primary technical reason for this is that our understanding of the concepts being explored in an evolving scientific domain change constantly, leading … more
Date: January 29, 1998
Creator: Critchlow, T.; Fidelis, K.; Ganesh, M.; Musick, R. & Slezak, T., LLNL
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Chapter 2: Genetic Variability in Nuclear Ribosomal and Chloroplast DNA in Utah (Juniperus Osteosperma) and Western (J. Occidentalis) Juniper (Cupressaceae): Evidence for Interspecific Gene Flow1

Description: Early studies of evolutionary change in chloroplast DNA indicated limited variability within species. This finding has been attributed to relatively low rates of sequence evolution and has been maintained as justification for the lack of intraspecific sampling in studies examining, relationships at the species level and above. However, documentation of intraspecific variation in cpDNA has become increasingly common and has been attributed in many cases to ''chloroplast capture'' following genet… more
Date: February 14, 1998
Creator: Terry, Randall G.; Tausch, Robin J. & Nowak, Robert S.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Meeting Report: The Twelfth International Mouse Genome Conference

Description: The annual International Mouse Genome Conference (IMGC) is where, scientifically speaking, classical mouse genetics meets the relative newcomer of genomics. The 12th meeting took place last October in the delightful Bavarian village of Garmisch-Partenkirchen, and we were greeted by the sight on the mountains of the first snowfall of the season. However the discussions left little time for exploration. Minds of participants in Garmisch were focused by a recent document produced by the NIH and by… more
Date: October 1, 1998
Creator: Manolakou, Katerina; Cross, Sally H.; Simpson, Eleanor H. & Jackson, Ian J.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

The DNA Files

Description: The DNA Files is a radio documentary which disseminates genetics information over public radio. The documentaries explore subjects which include the following: How genetics affects society. How human life began and how it evolved. Could new prenatal genetic tests hold the key to disease prevention later in life? Would a national genetic data base sacrifice individual privacy? and Should genes that may lead to the cure for cancer be privately owned? This report serves as a project update for the… more
Date: June 9, 1998
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Rapid mass spectrometric DNA diagnostics for assessing microbial community activity during bioremediation. 1998 annual progress report

Description: 'Biological activity has often been attributed to changes in pollutant profiles found in contaminated soils when abiotic processes actually caused pollutant removal. Careful evaluation of bioremediation necessitates that all transformation and removal pathways are anticipated so that the pathways are controlled or monitored. The author''s work is directed at evaluating a monitoring strategy that relies on the combined use of DNA diagnostic procedures and mass spectrometry as the detection schem… more
Date: June 1, 1998
Creator: Benner, W.H.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Novel mass spectrometry mutation screening for contaminant impact analysis. 1998 annual progress report

Description: 'The objective is to develop innovative mass spectrometry technology to achieve fast mutation screening from contaminated area and to reveal the linkage between gene mutation and contaminants. In this program, the author will try innovative approaches to improve mass resolution and detection efficiency for large DNA ions. Allel specific polymerase chain reaction will be coupled with mass spectrometry for rapid DNA mutation detection. The ultimate goal is to lead to the risk analysis of hazardou… more
Date: June 1, 1998
Creator: Chen, C.H.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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