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Reciprocal interactions between Beta1-integrin and epidermal growth factor in three-dimensional basement membrane breast cultures: A different perspective in epithelial biology

Description: Anchorage and growth factor independence are cardinal features of the transformed phenotype. Although it is logical that the two pathways must be coregulated in normal tissues to maintain homeostasis, this has not been demonstrated directly. We showed previously that down-modulation of {beta}1-integrin signaling reverted the malignant behavior of a human breast tumor cell line (T4-2) derived from phenotypically normal cells (HMT-3522) and led to growth arrest in a threedimensional (3D) basement… more
Date: September 30, 1998
Creator: Wang, F.; Weaver, V. M.; Petersen, O. W.; Larabell, C. A.; Dedhar, S.; Briand, P. et al.
Partner: UNT Libraries Government Documents Department
open access

Advanced Molecular and Microbial Techniques: a Complete Laboratory Notebook

Description: The purpose of this project is to produce a complete and thorough notebook that may be used to supplement laboratory coursework. Its intent is to be used primarily by the students to aid them in understanding background information and the proper laboratory procedures involved in various types of experiments. The laboratory notebook is a summation of all the experiments and procedures used in the six-credit hour Advanced Microbial and Molecular Biology (BIOL 5160) course offered during the summ… more
Date: May 1998
Creator: Brito-Rodriquez, Carmen Lydia
Partner: UNT Libraries
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Contemporary Biology Curriculum for Non-majors

Description: The proposed biology curriculum for non-majors has one main objective, namely to improve scientific literacy among college students. The National Science Education Standards defines scientific literacy as "the knowledge and understanding of scientific concepts and processes required for personal decision making, participation in civic and cultural affairs, and economic productivity". The suggested strategies to accomplish this goal are to limit the number of topics covered, introduce relevant s… more
Date: August 1998
Creator: Smallwood, Susan
Partner: UNT Libraries
open access

Multispectral UV Fluorescence Detection of a Dilute Constituent in an Optically Dense Matrix

Description: Multispectral UV fluorescence measurements were made of an optically dense medium (fetal bovine serum, FBS) spiked with sodium salicylate at concentrate ions from 0.2 to 500 pg/ml . Analysis of the spectra show that, depending on experimental conditions, reasonably good estimates of concentration can be obtained across the entire range of concentrate ions. Experimental conditions required for recovering these estimates are demonstrated.
Date: October 15, 1998
Creator: Chan, O.H.; Gray, P.C., Wehlburg, C.M.; Rubenstein, R.; Tisone, G.C. & Wagner, J.S.
Partner: UNT Libraries Government Documents Department
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Beryllium at Argonne East, past and present

Description: The focus of this presentation is the present activities at Argonne related to the control of beryllium exposure. However, since present activities involve some of the past uses of beryllium, the authors will review briefly the history as they have been able to resurrect it from records, memory and interviews with some of the people involved. The goal of the program is to identify past contaminated areas for remedial action, identify employees with past and current exposure who may benefit from… more
Date: July 1, 1998
Creator: Woodring, James L. & Davis, John T.
Partner: UNT Libraries Government Documents Department
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Advanced Techniques in Microbial and Molecular Biology: Laboratory Procedures for a Graduate Level Course

Description: Advanced laboratory techniques for Microbial and Molecular Biology at the graduate level are presented in this thesis. The procedures for the laboratory experiments are set forth in detail. This laboratory is conducted as two parts, each by a different professor. Part 1 covers the experiments conducted by Dr G.A.O. Donovan. These experiments include an introduction, staining procedures, biochemical reactions, mutagenesis experiment, essays,. preparation and analysis of plasmid DNA and various o… more
Date: August 1998
Creator: Kumar, Shalini
Partner: UNT Libraries
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Potential radiological doses associated with the disposal of petroleum industry NORM via landspreading. Final report, September 1998

Description: As a result of oil and gas production and processing operations, naturally occurring radioactive materials (NORM) sometimes accumulate at elevated concentrations in by-product waste streams. The primary radionuclides of concern in NORM wastes are radium-226 of the uranium-238 decay series, and radium-228, of the thorium-232 decay series. The production waste streams most likely to be contaminated by elevated radium concentrations include produced water, scale, and sludge. Scales and sludges rem… more
Date: December 1, 1998
Creator: Smith, Karen P.; Blunt, Deborah L. & Arnish, John J.
Partner: UNT Libraries Government Documents Department
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Genetic and biochemical analysis of solvent formation in Clostridium acetobutylicum

Description: The anaerobic organism Clostridium acetobutylicum has been used for commercial production of important organic solvents due to its ability to convert a wide variety of crude substrates to acids and alcohols. Current knowledge concerning the molecular genetics, cell regulation and metabolic engineering of this organism is still rather limited. The objectives are to improve the knowledge of the molecular genetics and enzymology of Clostridia in order to make genetic alterations which will more ef… more
Date: May 1, 1998
Creator: Bennett, George N. & Rudolph, Frederick B.
Partner: UNT Libraries Government Documents Department
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Use of a New Tandem Cation/Anion Exchange System With Clinical-Scale Generators Provides High Specific Volume Solutions of Technetium-99m and Rhenium-188

Description: In this paper the authors describe the first application of a simple and inexpensive post elution tandem cation-anion exchange column system which is based on generator elution with salts of weak acids such as ammonium acetate instead of saline solution to provide very high specific volume solutions of technetium-99m and rhenium-188 from clinical scale molybdenum-99/technetium-99m generator prepared from low specific activity (n,y) molybdenum-99, and tungsten-188/rhenium-188 generators, respect… more
Date: March 1, 1998
Creator: Knapp, F.R. Jr.; Beets, A. L.; Mirzadeh, S. & Guhlke, S.
Partner: UNT Libraries Government Documents Department
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Alfalfa leaf meal in wintering beef cow diets. Quarterly report, July 1, 1997--September 30, 1997

Description: One hundred dry pregnant cows (1389 lb) and twenty-four pregnant heifers (1034 lb) were assigned by calving date and body condition to one of four dietary treatments for a wintering period during their late gestation. Dietary treatments consisted of supplementing crude protein (CP) at 100 % or 120 % of the recommended intake using either soybean meal or alfalfa leaf meal (ALM) as the supplemental protein source. Cows were group fed (two replicate pens/treatment) while heifers were individually … more
Date: June 1, 1998
Creator: Zehnder, C.M.; Hall, J.M.; Brown, D.B. & DiCostanzo, A.
Partner: UNT Libraries Government Documents Department
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Alfalfa leaf meal in beef steer receiving diets. Quarterly report, July 1, 1997--September 30, 1997

Description: Two trials were conducted to study the effects of alfalfa leaf meal (ALM) in receiving diets of steers. In trial one, ninety-six medium frame, Angus and Angus cross steer calves (average initial weight 500 lb) were allotted to a heavy or light weight block and then randomly assigned to one of four dietary treatments for a 29-day receiving trial. In trial two, sixty medium frame, Angus and Angus cross steer calves (average initial weight 518 lb) were allotted to one of ten dietary treatments. Tr… more
Date: June 1, 1998
Creator: Zehnder, C. M.; DiCostanzo, A. & Smith, L. B.
Partner: UNT Libraries Government Documents Department
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Data mining

Description: This is the final report of a one-year, Laboratory Directed Research and Development (LDRD) project at the Los Alamos National Laboratory (LANL). The objective of this project was to develop and implement data mining technology suited to the analysis of large collections of unstructured data. This has taken the form of a software tool, PADMA (Parallel Data Mining Agents), which incorporates parallel data accessing, parallel scalable hierarchical clustering algorithms, and a web-based user inter… more
Date: December 31, 1998
Creator: Lee, Kenneth; Kargupta, Hillol; Stafford, Brian G.; Buescher, Kevin L. & Ravindran, Binoy
Partner: UNT Libraries Government Documents Department
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Radon source apportionment in the home, dosimetry and risk modeling. Final report, 1993--1997

Description: This research covered the following 3 topics in 4 years: (1) the source apportionment of {sup 222}Rn in the home; (2) the internal bronchial dosimetry of inhaled {sup 222}Rn decay products; and (3) the lung cancer risk from inhalation of the short lived decay products of {sup 222}Rn. A 4th year of support was appended to this grant with a switch in research effort to determine a method for long term measurement of the particle size distribution of the short lived decay products in homes.
Date: August 4, 1998
Creator: Harley, N. H.
Partner: UNT Libraries Government Documents Department
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Simultaneous two-photon excitation of photodynamic therapy agents

Description: The spectroscopic and photochemical properties of several photosensitive compounds are compared using conventional single-photon excitation (SPE) and simultaneous two-photon excitation (TPE). TPE is achieved using a mode-locked titanium:sapphire laser, the near infrared output of which allows direct promotion of non-resonant TPE. Excitation spectra and excited state properties of both type 1 and type 2 photodynamic therapy (PDT) agents are examined.
Date: January 1, 1998
Creator: Wachter, E. A.; Fisher, W. G.; Partridge, W. P.; Dees, H. C. & Petersen, M. G.
Partner: UNT Libraries Government Documents Department
open access

Marine Dead Zones: Understanding the Problem

Description: Very low levels of dissolved oxygen (hypoxia) in bottom-water "dead zones" are natural phenomena, but can be intensified by certain human activities. The largest hypoxic area affecting the United States is in the northern Gulf of Mexico near the mouth of the Mississippi River, but there are others as well.
Date: November 23, 1998
Creator: Dandelski, John R. & Buck, Eugene H.
Partner: UNT Libraries Government Documents Department
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Summaries of research projects for fiscal years 1996 and 1997, medical applications and biophysical research

Description: The Medical Applications and Biophysical Research Division of the Office of Biological and Environmental Research supports and manages research in several distinct areas of science and technology. The projects described in this book are grouped by the main budgetary areas: General Life Sciences (structural molecular biology), Medical Applications (primarily nuclear medicine) and Measurement Science (analytical chemistry instrumentation), Environmental Management Science Program, and the Small B… more
Date: February 1, 1998
Partner: UNT Libraries Government Documents Department
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Stress responses of subsurface bacteria. Final report, June 1, 1995--February 1, 1998

Description: Five questions were investigated in this research: (1) can subsurface bacteria and phylogenetically related surface strains be cultured on a single carbon/energy substrate; (2) is the behavior of subsurface bacteria the same as phylogenetically related surface strains when both are subjected to progressive, chronic carbon/energy source starvation; (3) which phyllogenetic groups survive better; (4) do other factors affect survival in the degree that phyllogeny does (5) do dormant forms appear du… more
Date: July 1, 1998
Creator: Chesbro, W.
Partner: UNT Libraries Government Documents Department
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Separations on water-ice. Final report

Description: This report focuses on processes to separate water frozen into ice. Research topics include the following: normal phase columnar chromatography; electrophoresis in a planar format; and zone melting type separations on a solid column of ice. Attempts were made to dope the emulsion with {beta}-cyclodextrin in order to separate commercially important chiral drugs such as Inderal.
Date: July 1, 1998
Creator: Dasgupta, P. K.
Partner: UNT Libraries Government Documents Department
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Literature review: Phytoaccumulation of chromium, uranium, and plutonium in plant systems

Description: Phytoremediation is an integrated multidisciplinary approach to the cleanup of contaminated soils, which combines the disciplines of plant physiology, soil chemistry, and soil microbiology. Metal hyperaccumulator plants are attracting increasing attention because of their potential application in decontamination of metal-polluted soils. Traditional engineering technologies may be too expensive for the remediation of most sites. Removal of metals from these soils using accumulator plants is the … more
Date: May 1, 1998
Creator: Hossner, L. R.; Loeppert, R. H.; Newton, R. J. & Szaniszlo, P. J.
Partner: UNT Libraries Government Documents Department
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An editing environment for DNA sequence analysis and annotation

Description: This paper presents a computer system for analyzing and annotating large-scale genomic sequences. The core of the system is a multiple-gene structure identification program, which predicts the most probable gene structures based on the given evidence, including pattern recognition, EST and protein homology information. A graphics-based user interface provides an environment which allows the user to interactively control the evidence to be used in the gene identification process. To overcome the… more
Date: December 31, 1998
Creator: Uberbacher, E.C.; Xu, Y.; Shah, M.B.; Olman, V.; Parang, M. & Mural, R.
Partner: UNT Libraries Government Documents Department
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Response of vegetation to carbon dioxide. Growth, yield and plant water relationships in sweet potatoes in response to carbon dioxide enrichment 1986

Description: In the summer of 1985, under the joint program of US Department of Energy, Carbon Dioxide Division, and Tuskegee University, experiments were conducted to study growth, yield, photosynthesis and plant water relationships in sweet potato plants growth in an enriched CO{sub 2} environment. The main experiment utilized open top chambers to study the effects of CO{sub 2} and soil moisture on growth, yield and photosynthesis of field-grown plants. In addition, potted plants in open top chambers were… more
Date: August 1, 1998
Partner: UNT Libraries Government Documents Department
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