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in subjects: "In-Situ Hybridization"  
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A sequence-based survey of the complex structural organization of tumor genomes

Description: The genomes of many epithelial tumors exhibit extensive chromosomal rearrangements. All classes of genome rearrangements can be identified using End Sequencing Profiling (ESP), which relies on paired-end sequencing of cloned tumor genomes. In this study, brain, breast, ovary and prostate tumors along with three breast cancer cell lines were surveyed with ESP yielding the largest available collection of sequence-ready tumor genome breakpoints and providing evidence that some rearrangements may b… more
Date: April 3, 2008
Creator: Collins, Colin; Raphael, Benjamin J.; Volik, Stanislav; Yu, Peng; Wu, Chunxiao; Huang, Guiqing et al.
Partner: UNT Libraries Government Documents Department
open access

Chromosomal mosaicism in mouse two-cell embryos after paternal exposure to acrylamide

Description: Chromosomal mosaicism in human preimplantation embryos is a common cause ofspontaneous abortions, however, our knowledge of its etiology is limited. We used multicolor fluorescence in situ hybridization (FISH) painting to investigate whether paternally-transmitted chromosomal aberrations result in mosaicism in mouse 2-cell embryos. Paternal exposure to acrylamide, an important industrial chemical also found in tobacco smoke and generated during the cooking process of starchy foods, produced sig… more
Date: October 14, 2008
Creator: Marchetti, Francesco; Bishop, Jack; Lowe, Xiu & Wyrobek, Andrew J
Partner: UNT Libraries Government Documents Department
open access

Validation of DNA probes for molecular cytogenetics by mapping onto immobilized circular DNA

Description: Fluorescence in situ hybridization (FISH) is a sensitive and rapid procedure to detect gene rearrangements in tumor cells using non-isotopically labeled DNA probes. Large insert recombinant DNA clones such as bacterial artificial chromosome (BAC) or P1/PAC clones have established themselves in recent years as preferred starting material for probe preparations due to their low rates of chimerism and ease of use. However, when developing probes for the quantitative analysis of rearrangements invo… more
Date: December 16, 2008
Creator: Greulich-Bode, Karin; Wang, Mei; Rhein, Andreas; Weier, Jingly & Weier, Heinz-Ulli
Partner: UNT Libraries Government Documents Department
open access

Chromosome-specific DNA Repeat Probes

Description: In research as well as in clinical applications, fluorescence in situ hybridization (FISH) has gained increasing popularity as a highly sensitive technique to study cytogenetic changes. Today, hundreds of commercially available DNA probes serve the basic needs of the biomedical research community. Widespread applications, however, are often limited by the lack of appropriately labeled, specific nucleic acid probes. We describe two approaches for an expeditious preparation of chromosome-specific… more
Date: March 16, 2006
Creator: Baumgartner, Adolf; Weier, Jingly Fung & Weier, Heinz-Ulrich G.
Partner: UNT Libraries Government Documents Department
open access

Molecular stress response in the CNS of mice after systemic exposureto interferon-alpha, ionizing radiation and ketamine

Description: We previously showed that the expression of troponin T1 (Tnnt 1) was induced in the central nervous system (CNS) of adultmice 30 min after treatment with ketamine, a glutamate N-methyl-D-aspartic acid (NMDA) receptor antagonist. We hypothesized that Tnnt 1 expression may be an early molecular biomarker of stress response in the CNS of mice. To further evaluate this hypothesis, we investigated the regional expression of Tnnt 1 in the mouse brain using RNA in situ hybridization 4 h after systemic… more
Date: March 3, 2009
Creator: Lowe, Xiu R.; Marchetti, Francesco; Lu, Xiaochen & Wyrobek, Andrew J.
Partner: UNT Libraries Government Documents Department
open access

Molecular cytogenetic characterization of a human thyroid cancercell line

Description: The incidence of papillary thyroid carcinoma (PTC) increases significantly after exposure of the head and neck region to ionizing radiation, yet we know neither the steps involved in malignant transformation of thyroid epithelium nor the specific carcinogenic mode of action of radiation. Such increased tumor frequency became most evident in children after the 1986 nuclear accident in Chernobyl, Ukraine. In the twelve years following the accident, the average incidence of childhood PTCs (chPTC) … more
Date: January 4, 2006
Creator: Weier, Heinz-Ulrich G.; Tuton, Tiffany B.; Ito, Yuko; Chu, LisaW.; Lu, Chung-Mei; Baumgartner, Adolf et al.
Partner: UNT Libraries Government Documents Department
open access

Molecular cytogenetic analysis of human blastocysts andcytotrophoblasts by multi-color FISH and Spectra Imaging analyses

Description: Numerical chromosome aberrations in gametes typically lead to failed fertilization, spontaneous abortion or a chromosomally abnormal fetus. By means of preimplantation genetic diagnosis (PGD), we now can screen human embryos in vitro for aneuploidy before transferring the embryos to the uterus. PGD allows us to select unaffected embryos for transfer and increases the implantation rate in in vitro fertilization programs. Molecular cytogenetic analyses using multi-color fluorescence in situ hybri… more
Date: February 8, 2006
Creator: Weier, Jingly F.; Ferlatte, Christy; Baumgartner, Adolf; Jung,Christine J.; Nguyen, Ha-Nam; Chu, Lisa W. et al.
Partner: UNT Libraries Government Documents Department
open access

Evolutionary expansion and divergence in a large family of primate-specific zinc finger transcription factor genes

Description: Although most genes are conserved as one-to-one orthologs in different mammalian orders, certain gene families have evolved to comprise different numbers and types of protein-coding genes through independent series of gene duplications, divergence and gene loss in each evolutionary lineage. One such family encodes KRAB-zinc finger (KRAB-ZNF) genes, which are likely to function as transcriptional repressors. One KRAB-ZNF subfamily, the ZNF91 clade, has expanded specifically in primates to compri… more
Date: September 28, 2005
Creator: Hamilton, A T; Huntley, S; Tran-Gyamfi, M; Baggott, D; Gordon, L & Stubbs, L
Partner: UNT Libraries Government Documents Department
open access

In situ quantification of genomic instability in breast cancer progression

Description: Genomic instability is a hallmark of breast and other solid cancers. Presumably caused by critical telomere reduction, GI is responsible for providing the genetic diversity required in the multi-step progression of the disease. We have used multicolor fluorescence in situ hybridization and 3D image analysis to quantify genomic instability cell-by-cell in thick, intact tissue sections of normal breast epithelium, preneoplastic lesions (usual ductal hyperplasia), ductal carcinona is situ or invas… more
Date: May 15, 2003
Creator: Ortiz de Solorzano, Carlos; Chin, Koei; Gray, Joe W. & Lockett, Stephen J.
Partner: UNT Libraries Government Documents Department
open access

A system for combined three-dimensional morphological and molecular analysis of thick tissue specimens

Description: We present a new system for simultaneous morphological and molecular analysis of thick tissue samples. The system is composed of a computer assisted microscope and a JAVA-based image display, analysis and visualization program that allows acquisition, annotation, meaningful storage, three-dimensional reconstruction and analysis of structures of interest in thick sectioned tissue specimens. We describe the system in detail and illustrate its use by imaging, reconstructing and analyzing two compl… more
Date: April 25, 2002
Creator: Fernandez-Gonzalez, Rodrigo; Jones, Arthur; Garcia-Rodriguez, Enrique; Yuan Chen, Ping; Idica, Adam; Lockett, Stephen J. et al.
Partner: UNT Libraries Government Documents Department
open access

Identification of a third protein 4.1 tumor suppressor, protein 4.1R, in meningioma pathogenesis

Description: Meningiomas are common tumors of the central nervous system, however, the mechanisms under lying their pathogenesis are largely undefined. Two members of the Protein 4.1 super family, the neuro fibromatosis 2 (NF2) gene product (merlin/schwannomin) and Protein 4.1B have been implicated as meningioma tumor suppressors. In this report, we demonstrate that another Protein 4.1 family member, Protein 4.1R, also functions as a meningioma tumor suppressor. Based on the assignment of the Protein 4.1R g… more
Date: June 11, 2003
Creator: Robb, Victoria A.; Li, Wen; Gascard, Philippe; Perry, Arie; Mohandas, Narla & Gutmann, David H.
Partner: UNT Libraries Government Documents Department
open access

A BAC-based physical map of the Drosophila buzzatii genome

Description: Large-insert genomic libraries facilitate cloning of large genomic regions, allow the construction of clone-based physical maps and provide useful resources for sequencing entire genomes. Drosophilabuzzatii is a representative species of the repleta group in the Drosophila subgenus, which is being widely used as a model in studies of genome evolution, ecological adaptation and speciation. We constructed a Bacterial Artificial Chromosome (BAC) genomic library of D. buzzatii using the shuttle vec… more
Date: March 18, 2005
Creator: Gonzalez, Josefa; Nefedov, Michael; Bosdet, Ian; Casals, Ferran; Calvete, Oriol; Delprat, Alejandra et al.
Partner: UNT Libraries Government Documents Department
open access

Rapid evolution of a recently retroposed transcription factor YY2 in mammalian genomes

Description: YY2 was originally identified due to its unusual similarity to the evolutionarily well conserved, zinc-finger gene YY1. In this study, we have determined the evolutionary origin and conservation of YY2 using comparative genomic approaches. Our results indicate that YY2 is a retroposed copy of YY1 that has been inserted into another gene locus named Mbtps2 (membrane-bound transcription factor protease site 2). This retroposition is estimated to have occurred after the divergence of placental mam… more
Date: November 11, 2005
Creator: Luo, C.; Lu, X.; Stubbs, L. & Kim, J.
Partner: UNT Libraries Government Documents Department
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