High-Sensitivity Single-Molecule Fluorescence Detection in Theory and Practice
Description: The number of emitted photons that can be obtained from a fluorophore increases with the incident light intensity and the duration of illumination. However, saturation of the absorption transition and photodestruction place natural limits on the ultimate signal-to-noise ratio that can be obtained. Equations have been derived to describe the fluorescence-to-background-noise ratio in the presence of saturating light intensities and photodestruction. The fluorescence lifetime and the photodestruct… more
Creator: Mathies, R. A.; Peck, K. & Stryer, L.
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