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FY02 CBNP Annual Report: Discovery of DNA Signature of Biothreat Detection Using Suppression Subtractive Hybridization

Description: Our goal is to develop robust DNA signatures for rapid and specific DNA-based detection platforms that can be employed by CBNP to detect a wide range of potential agents. Our approach has resulted in highly specific DNA signatures for Yersina pestis, Bacillus anthracis and Brucella species. Furthermore, this approach can be applied to any genome (even uncharacterized ones), which facilitates DNA signature development for detection of newly emerging pathogens. We are using suppression subtractive hybridization (SSH) as a tool to define large DNA regions specific to multiple biothreat pathogens by comparing them to genomes of the most closely related organisms. This approach has become increasingly accurate as we continue to find new, distinctive strains and ever-closer near-neighbors. With the huge costs incurred by whole genome sequencing, it is not possible to sequence each new bacterial genome. However, it is completely practical to identify genome differences in the laboratory using SSH, and becomes especially useful when comparing new strains to previously sequenced genomes.
Date: November 19, 2002
Creator: Andersen, G L & Radnedge, L

Melt Rate Testing for the DWPF: Summary of FY02 Testing

Description: A study performed in FY01 recommended that the Defense Waste Process Facility (DWPF) replace Frit 200 with Frit 320 for the processing of sludge batch 2 (SB2) to improve melt rate (Lambent et al., 2001) contingent upon additional testing which included slurry feeding. The FY02 melt rate program was developed to support this recommendation as well as to investigate alternative methods of improving melt rate above and beyond a change in frit composition. The integrated program was primarily based on a sound testing methodology from which the frit change was recommended. In addition, the program included the development of additional tools to provide further insight into melt rate enhancements. The overall strategy of the FY02 testing program was to design suites of melt rate tests based on recommendations from previous work that provided insight into other methods of improving melt rate for the DWPF. The objectives of the FY02 testing program included how melt rate might be influenced by increases in waste loading, differences in frit particle size, the use of batch chemicals rather than a pre-fabricated frit, and the impact of uranium. This report summarizes the equipment development and setup, procedures, and results of this testing, and includes recommendations on the processing of SB2 with Frit 320 and future research.
Date: February 19, 2003
Creator: Lorier, T.H.

Water Infrastructure Financing: History of EPA Appropriations

Description: This report summarizes, in chronological order, congressional activity to fund items in the State and Tribal Assistance Grants (STAG) account since 1987. In appropriations legislation, funding for EPA (Environmental Protection Agency) wastewater assistance is contained in the measure providing funds for the Department of the Interior, Environment, and Related Agencies.
Date: January 19, 2006
Creator: Copeland, Claudia

The Mid-Session Review of the President's Budget: Timing Issues

Description: This report provides information on the timing of submission of the mid-session review for FY1980-FY2009, a 30-year period that covers all or part of the administrations of five Presidents, applying to the last two years of the Carter Administration, and the full terms of the Reagan, George H. W. Bush, Clinton, and George W. Bush Administrations.
Date: August 19, 2008
Creator: Keith, Robert