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The Effect of Ethanol on Cardiac Activity and Brain Respiration in Chick Embryos

Description: This study concerned the effect of ethanol on cardiac activity and brain respiration in chick embryos. Ethanol dosages tested ranged from 1.0 mg to 4.0 mg/g weight. Each experiment lasted at least 150 minutes. Cardiac activity in terms of total waveform energies was integrated and printed out for plotting and analysis. The embryonic heart rates were simultaneously determined from physical graph tracings. The embryonic brain respiration was measured using a differential microrespirometer. The effect of ethanol on cardiac activity was one of slight (10 to 13), but statistically significant (p<.05) rate depression. The brain slices exhibited a marked, immediate, and irreversible decrease (39 to 89%) in oxygen consumption at both ethanol dosages. The data indicated that chick embryonic brain tissues were more susceptible to alcohol effects than cardiac tissue. Therefore, the mental abnormalities seen in the offspring of alcoholic mothers may be more cerebral in nature than cardiovascular.
Date: August 1976
Creator: Newman, James J.

Serological Relationships of Azotobacter Species

Description: In order to clarify the taxonomic problems which exist among the group azotobacter by serological method, 24 cultures of Azotobacter including 9 species were tested as antigens in Ouchterlony plates against 3 different antisera obtained from rabbits immunized with A. chroococcum ACl6, A. macrocytogenes St.M. and A. vinelandii 12837, respectively. The results showed more cross reactivity in intraspecies reactions than in interspecies ones and indicated the presence of a common immunogenic determinant. This work presents the results which suggest the effecient classification of Azotobacter by Ouchterlony comparisons of corresponding antigens in different species.
Date: December 1976
Creator: Chang, Chyan-chuu