UNT Libraries - 11 Matching Results

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Aryl Hydrocarbon Hydroxylase and Sixteen Alpha Hydroxylase in Cultured Human Lymphocytes

Description: Cultured human lymphocytes may be assayed for aryl hydrocarbon hydroxylase (AHH) in whole cell preparations. The optimum assay conditions are pH 8.5, and 1.5 mM Mg++. The reaction is linear with time and cell number, and is inhibited by CO. Estradiol may inhibit induction of AHH by 3-methylcholanthrene, but is a poor competitor for the enzyme. A Caucasian population was assayed for AHH activity. The distribution was lognormal; no difference was found in cultured cells from males and females or … more
Date: December 1975
Creator: Coomes, Marguerite L.
Item Type: Refine your search to only Thesis or Dissertation
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Differences in Protein Constituents of Some Azotobacter Species

Description: This study used polyacrylamide gel electrophoresis to study the acid-phenol soluble proteins of five strains (A. vinelandii 12837, A. vinelandii 0, A. chroococcum 8004, A. macrocytogenes 8702, A. tumefaciens) of bacteria grown on Burk's nitrogen-free media, Trypticase Soy Broth, and 0.3% butanol medium. The results showed that the protein patterns can be used for the identification and possibly the taxonomic classification of the Azotobacter. The change of phenotype of the bacteria in different… more
Date: August 1975
Creator: Hsu, Li-Chu Yao
Item Type: Refine your search to only Thesis or Dissertation
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Effect of Inorganic Phosphate on the Morphology and Poly-β-Hydroxybutyrate Content of Azotobacter Vinelandii Atcc 12837

Description: This study was designed to determine the effect of inorganic phosphate in the growth media of A. vinelandii on the synthesis and accumulation of poly-β-hydroxybutyrate (PHB) and cellular morphology. Burk's media with no added phosphate and ten times normal phosphate resulted in altered cells, lower levels of protein, and decreased cell populations. PHB accumulation was much higher in the cultures with no added phosphate; however, the polymer granules differed in appearance from those of normal … more
Date: May 1975
Creator: Collins, Patricia Ann
Item Type: Refine your search to only Thesis or Dissertation
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Evolutionary Genetics of Three Semispecies of Wood Rats--Neotoma Albigula, Neotoma Micropus, and Neotoma Floridana

Description: Electrophoretic variation in 18 proteins encoded by 20 autosomal loci was used to compare the genetic relationships of 19 natural populations representing three species of the subgenus Neotoma. Of the 20 loci examined nine were monomorphic and fixed for the same allele in all populations. No more than seven loci were polymorphic within a single population. Genetic variability was expressed as the proportion of loci heterozygous in the average individual of a population. Heterozygosity in the th… more
Date: December 1975
Creator: Nejtek, Michael E.
Item Type: Refine your search to only Thesis or Dissertation
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Genic Differentiation and Evolution in the Ground Squirrel Subgenus Ictidomys (Spermophilus)

Description: The genetic structure of 26 natural populations of three species (S. tridecemlineatus, S. mexicanus, and S. spilosoma) of the Ictidomys subgenus of ground squirrels was analyzed using chromosomal and electrophoretic techniques. Chromosomal variation was not observed in S. mexicanus, and only slight karyotypic variation was found in the other two species. Chromosomal evidence indicated hybridization between S. tridecemlineatus and S. mexicanus, placing these species within the classical definiti… more
Date: December 1975
Creator: Cothran, E. Gus, 1951-
Item Type: Refine your search to only Thesis or Dissertation
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Induction of 16α Hydroxylase in Human Cultured Lymphocytes

Description: A method is presented for 160hydroxylase (SAH) induction in cultured human lymphocytes. SAH, a microsomal-associated enzyme, effects the oxidative conversion of 17pestradiol to estriol, which competes for cytoplasmic binding sites. 17,-estradiol and estrone are known mammary carcinogens, while estriol and its epimers have been suggested to have anticarcinogenic properties. To substantiate genetic variations of hydroxylase activity, an analysis of estrogen-induced cultured human lymphocytes was … more
Date: December 1975
Creator: Muijsson, Ingrid E.
Item Type: Refine your search to only Thesis or Dissertation
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Pharmacological, Temperature, and Electrogram Studies on the Posterior Lymph Heart of the Bullfrog

Description: In view of the discrepancies and conflicts produced by previous studies on amphibian lymph hearts, a study was initiated to reinvestigate the pharmacological, temperature, and electrical aspects of lymph heart physiology. Bullfrogs were chosen as the experimental animal, All lymph heart responses to experimentation were physiographically recorded as myograms and electrograms. The results are in agreement with previous studies on some aspects and in conflict on others. From the results obtained,… more
Date: May 1975
Creator: Oberndorfer, Carol E.
Item Type: Refine your search to only Thesis or Dissertation
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The Population Dynamics and Trophic Relationships of Seven Species of Fish in a Small Southwestern Pond, with Special Attention Toward Young-of-the-Year Fish

Description: Production rates of seven species of fish, along with food-web interactions, were determined for one year in a pond. Production of fish in the pond amounted to 208.9 q/m2/yr. Over 95% of the production occurred in the summer, with Y of Y fish contributing 83.8%. There were two food-web interactions observed in Y of Y fishes: crappie vs. bass and bullhead vs. sunfish. When Y of Y fish shifted to benthic diets in the spring, the small biomass of benthos available could not sustain the larger bio… more
Date: August 1975
Creator: Jones, Fredrick V.
Item Type: Refine your search to only Thesis or Dissertation
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Purification and Characterization of Aldolase From Ambystoma Tigrinum

Description: The muscle aldolase from Ambystoma tigrinum has been purified 73-fold to a final specific activity of 13.2 units per mg. The purified enzyme appeared to be homogenous by ultracentrifugation and electrophoretic criteria. A molecular weight of 159,000 + 1000 was determined by gel filtration on Sephadex G-200 and high speed sedimentation equilibrium ultracentrifugation. The enzyme migrated identically with rabbit muscle aldolase when subjected to sodium dodecyl sulfate polyacrylamide gel electroph… more
Date: December 1975
Creator: Woolever, Dorothy J.
Item Type: Refine your search to only Thesis or Dissertation
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Purification and Characterization of Ascaris Suum Hexokinase

Description: Hexokinase has been found in a soluble and a bound form in homogenates of Ascaris suum muscle. Cellulose acetate electrophoresis, isoelectric focusing, and ion exchange chromatography confirmed the presence of only one molecular form of hexokinase in this muscle. A procedure for purifying hexokinase from Ascaris muscle has been developed. The physical and catalytic properties of the enzyme are comparable to mammalian hexokinases. The enzyme is a monomer with a molecular weight of 100,000 as det… more
Date: August 1975
Creator: Supowit, Scott C.
Item Type: Refine your search to only Thesis or Dissertation
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The Stoneflies (Plecoptera) of Texas

Description: An illustrated key to the adults and known nymphs of Texas Plecoptera is provided. Species accounts, including geographic distribution within Texas, and biological notes are given. Of the twenty-seven species of Plecoptera known from Texas, six are new state records. TWo species new to science, Isoperla jewetti and Isoperla coushatta are described. Taeniopteryx starki Stewart and Szczytko, Zealeuctra arnoldi Ricker and Ross, and Zealeuctra hitei Ricker and Ross are endemic to the Edwards Platea… more
Date: May 1975
Creator: Szczytko, Stanley W.
Item Type: Refine your search to only Thesis or Dissertation
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