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A Study of Some Congenital Anomalies of the Hands and Feet
This paper has been undertaken, first, to contribute several more cases of various congenital anomalies to the literature on human genetics, and, second, by an analysis of the genealogies, to determine the possible modes of transmission of these anomalies.
In Vitro Determination of the Cellulose-Decomposing Rates of Twelve Denton County, Texas Soils
In this study twelve types of top soil were collected under aseptic conditions. The cellulose-decomposing rates of these were compared in order to determine the relative rates in the cellulose-decomposing potential of the microorganisms involved. Furthermore, this investigation is designed to acquire pertinent information on the rate at which natural cellulose materials are returned to available plant food.
The Inhibition of Fungal Contaminants in Cultures of Mycobacterium Tuberculosis
The problem of conatmination in culture media for Mycobacterium tuberculosis has not been solved completely, and for this reason the work herein presented was carried out. In this work experiments were made testing the effect of actidione in inhibiting certain ones of the higher fungi.
The Adaptability and Present Status of Andropogon Ischaemum L. in Denton County, Texas
This investigation has been made with reference to the adaptability of Andropogon ischaemum, King Ranch bluestem, to various soils, various slopes, and various erosion conditions in Denton County.
Development and Application of an Assessment Protocol for Watershed Based Biomonitoring
With numerous bioassessment methodologies available, a regional protocol needs to be developed to ensure that results are comparable. A regional assessment protocol was developed that includes collecting five benthic macroinvertebrate samples, identifying organisms to genus, and calculating the following metrics: Number of Taxa, Total Number of Individuals, Simpson's Diversity Index, Shannon's Diversity Index, Percent Contribution of Dominant Taxa, Hilsenhoffs Biotic Index, and Percent Contribution of Dipterans. Once the protocol was developed, it was used to assess the Bayou Chico tributaries and watershed. All three tributaries had been significantly impacted by human activity as had the watershed as a whole. This study indicates that a regional protocol could be developed and is appropriate for biomonitoring at the watershed scale.
The Sensitivity of Human Blood Plasma to the Coagulase Enzyme Secreted by Members of the Genus Micrococcus
The problem in this investigation consisted of, first, the isolation from human sources and identification of thirteen cultures of typical micrococci to be used as test organisms; second, the acquisition of blood plasma from thirty different human beings; and third, the determination of the possibility of individual variation in sensitivity of blood plasma to the micrococci used as test organisms as revealed by the coagulase test.
The Isolation, Cultivation and Testing of Organisms Anatagonistic to a Streptomycin Resistant Strain of Pseudomonas Aeruginosa
The problem of finding an efficient antibiotic against Pseudomonas aeruginosa which can be used in the clinical treatment of genito-urinary tract infections resistant to treatment by streptomycin has not yet been solved. Therefore, this problem has consisted of first, the acquisition of possible inhibitors of the streptomycin resistant strain of Pseudomonas aeruginosa; second, the selection and identification of those which show a marked antagonism toward this organism; third, the determination of the antibiotic spectra of the inhibitors; fourth, the determination of whether the streptomycin resistant strain could also acquire a resistance to the antibiotic produced by its inhibitors; and last, an attempt to evaluate the therapeutic possibilities of the antibiotics demonstrated.
Life History and Case-building Behavior of Molanna Tryphena Betten (Trichoptera: Molannidae) in Two East Texas Spring-fed Streams
The life history and case-building behavior of Molanna tryphena from two spring-fed tributaries in East Texas were studied from January 1997 to May 1998.
Forest Landscape Dynamics: a Semi-Markov Modeling Approach
A transition model (MOSAIC) is used to describe forest dynamics at the landscape scale. The model uses a semi-Markov framework by considering transition probabilities and Erlang distributed holding times in each transition. Parameters for the transition model are derived from a gap model (ZELIG). This procedure ensures conceptual consistency of the landscape model with the fine scale ecological detail represented by the forest gap model. Spatial heterogeneity in the transition model is driven by maps of terrain with characteristics contained in a Geographic Information System (GIS) database. The results of the transition model simulations, percent cover forest type maps, are exported to grid-maps in the GIS. These cover type maps can be classified and used to describe forest dynamics using landscape statistics metrics. The linkage model-GIS enhances the transition model spatial analytical capabilities. A parameterization algorithm was developed that takes as input gap model tracer files which contain the percent occupation of each cover type through time. As output, the algorithm produces a file that contains the parameter values needed for MOSAIC for each one of the possible transitions. Parameters for the holding time distribution were found by calculating an empirical estimate of the cumulative probability function and using a non-linear least squares method to fit this estimate to an Erlang distribution. The algorithm provided good initial estimates of the transitions parameters that can be refined with few additional simulations. A method for deriving classification criteria to designate cover types is presented. The method uses cluster analysis to detect the number and type of forest classes and Classification and Regression Tree (CART) analysis to explain the forest classes in term of stand attributes. This method provided a precise and objective approach for forest cover type definition and classification. The H. J. Andrews forest in Oregon was used to demonstrate the ...
Impacts of the Pyrethroid Insecticide Cyfluthrin on Aquatic Invertibrate Populations in Outdoor Experimental Tanks
The chemical fate and biological impacts of cyfluthrin in aquatic ecosystems were investigated using microcosms (1.9 m^3 concrete tanks) during 1989. Results were compared to a concurrent pesticide registration study using mesocosms (634.7 m^3 earthen ponds). Ten spray drift and five soil runoff simulations were conducted. Pesticide loadings were scaled by system volume, with the same experimental design in ponds and microcosms. Aqueous cyfluthrin concentrations and sediment residue values were generally higher in microcosms, while aqueous half-life was shorter in the smaller systems.
The Antibiotic Effect of Pigmented Actinomycetes
This thesis attempts, first, to correlate pigmentation of actinomycetes with the degree of antibiocity; second, to establish the most favorable means of producing a rich luxuriant pigmentation in the individual organism studied by enriching favorable media with specific types of proteins and amino acids.
Age, Growth, and Food Habits of the Largemouthed Black Bass (Micropterus Salmoides) and the Spotted Bass (Micropterus Puctulatus SSP) in North and East Texas Lakes
This study is based on the examinations and analyses of 388 largemouthed black bass (Micropterus salmoides) and 118 spotted black bass (Micropterus puctulatus ssp.) which were collected over a period of three years from 9 lakes in the North-Central and East Texas areas. The results should show the following: (1) the average age of the black bass in each of the nine reservoirs in Texas; (2) the growth rate of the bass in these reservoirs; (3) the feeding habits of the bass in all reservoirs studied; (4) a comparison of the growth rates of the largemouthed and spotted basses; and (5) a comparison of the growth rates of bass from Texas and Oklahoma.
A Determination of the Effects of Various Concentrations of Sodium Chloride upon the Growth of Three Species of Bacteria
The problem in this investigation is to determine the effects of various concentrations of sodium chloride upon the growth of three species of bacteria. An effort has been made to solve this problem, not only by a study of the relevant literature, but also by laboratory research consisting of cultivation and observation of the three organisms which were arbitrarily chosen for this study.
Landscape Ecological Characteristics of Habitat of the Red-cockaded Woodpecker
Geographic information systems (GIS) technology was used to analyze factors influencing habitat use by an endangered species, the red-cockaded woodpecker. The study area was the western part of the Raven Ranger District of the Sam Houston National Forest, Texas. The factors considered included both structural characteristics and spatial relationships among stands of trees in the habitat.
A Grassland Evaluation of Eastland County, Texas
This investigation has had for its purpose the determination of first, all members of the Gramineae (Grass) family found in the county; second, the incidence of each species with the various types of soil; third, the grazing value of each species; fourth, the life span of the parent plants; fifth, the present grassland status; and sixth, the potentialities of developing desirable grassland.
The Antibiotic Properties of the Oleoresins of Twenty-Five Common Garden Vegetables
The purpose of this problem is to determine the presence and extent of antibiotic materials as found in the oleoresins of a selected group of garden vegetables. The problem has consisted of, first, the collection and preparation of specimens of twenty-five commonly used garden vegetables; second, the extraction of the oleoresins from these; third, the determination of the inhibitory and other effects of these oleoresins against several strains of selected gram-positive and gram-negative bacteria; and fourth, the evaluation of the potentialities of these oleoresins with regard to their future use as medicinal prophylactics and therapeutics.
Survival and Growth of Bacteria in Chlorine Treated Water
In this problem, an attempt was made to determine the fate of various species of bacteria which had previously been isolated from other sources when inoculated into Denton tap water.
A Vegetative Key to the Grasses of Erath County, Texas
"The orientation of this study began with a collection of the grasses of Erath County during their flowering periods. Collections were made in the Spring and Fall of 1950 and 1951 to obtain both vernal and autumnal grasses. Identifications were made from the grass floras previously mentioned and checked against specimens in the herbarium of North Texas State College for verification. In addition, the list was checked by W.A. Turney, Conservationist, United States Department of Agriculture of Dublin, Texas. Finally, the list was compared with that prepared by W.M. Cory for the plant areas of which Erath County is a part. On the basis of the above identifications, a critical study of the vegetative characteristics was made and a key based on these features prepared. The width of the blad and characteristics of the ligule, roots, culms, and sheaths were used to distinguish each of the eighty-six specimens found in the collection area. A drawing was made of the longitudinal section of the juncture of the blade and the sheath of each grass. This diagnostic section includes the collar, ligule, a part of the blade and sheath, and auricles, if present."-- leaf 5.
Effect of Cortisone Injections on Complement Titers of Guinea Pigs
As complement is now believed to have some function in immunity it becomes of interest to determine what effect, if any, cortisone may have upon complement concentration in animal sera.
Degradation of Hexadecanol by Certain Bacterial Species
The purpose of this thesis is to determine the effect of hexadecanol on the populations of Pseudomonas and Alcaligenes species in reservoirs and determine their ability to utilize this compound as a carbon source.
Microflora in Prepared Foods Dispensed from Eating Establishments in Dallas, Texas
A bacteriological study was made of a number of prepared foods that were bought ready-to-eat, for home consumption, from several food establishments in Dallas, Texas. The purpose of this study was to show whether these foods could be a potential source of food poisoning; whether there are any particular foods that should have greater care than others in order to protect their quality; and whether the bacteriological contamination could have been prevented by strict observance of the city regulations for handling foods.
Changes which Occur in Components C'3 and C'4 in Guinea Pig Complement after Injection of an Antigen
This thesis describes an experiment involving guinea pigs' blood and the changes that occurred in the serum with the injection of an antigen. The serum was analyzed in complement titration tests.
A Study of Selected Factors Influencing Science Majors Toward Science Careers
"The purpose of this study is to gather information concerning some factors which may have operated to influence science majors in North Texas State College toward science careers."--4.
The Effects of Common Electrolytes on Growth and Development of Selected Species of Aquatic Actinomycetes
The role that aquatic actinomycetes play, in the production of tastes and odors in water supplies has been investigated since 1948. The ability of these organisms to produce by-products in lakes and streams, which renders the water unpalatable, is of considerable public health importance. It is desirable that the waterworks industry has as much information as possible concerning the factors that contribute to the growth of these organisms. Since it appears that the aquatic actinomycetes may be isolated from most fresh-water sources, the problem of diversified environments and nutritional requirements offers an excellent field of investigation. The fresh-waters of the world contain variable quantities of electrolytes that may determine in part the biological activity of these organisms. The unsolved questions in this instance are concerned with the electrolytes present and their quantitative effects on the growth and development of these forms.
A Study of a Selected Group of Science Related Characteristics of Non-Science Majors
The purpose of this study is to gather information related to the characteristics of two groups of undergraduate non-science majors; namely, those students who once considered science as a career and subsequently changed to a non-science major and those who have never considered a career in science. It is frequently observed that children in the elementary school have an intense interest in science. One of the principal concerns of this study is how and for what reasons has the interest in science changed between elementary school and college? It is also the purpose of this study to gather information related to the attitudes of these college students toward the science courses they have had in high school and toward their science teachers. In attempting to arrive at an answer, the group who once considered a science career and those who have never considered an occupational choice in a field of science will be compared in the following areas: 1. General information such as sex, age, size of high school attended, rank within their graduating classes, college classification and military experiences. 2. Occupations and hobbies of parents. 3. Experiences which may influence attitudes toward science such as elementary school science, high school science courses, high school science teachers, attitudes toward science requirements, high school courses liked most, high school courses liked least, and membership in science clubs. 4. College science courses completed. 5. Extent of the interviewees' present interest in science, Finally, the circumstances surrounding the tentative choice of a science career by those who once considered such a career will be discussed.
Studies on the Nutrition of Rhodospirillum Rubrum
The purpose of this study has been to examine the nutritional requirements of Rhodospirillum rubrum and, on the basis of the results, develop a culture medium which could be use to promote more rapid and abundant growth facilitating the laboratory cultivation and observations of the properties so exhibited.
Effects of Antigen Injection on Titer of C'3 and C'4 Complement Components of Rat Serum
This work attempts to establish some phenomenon of a rise in titer of C'3 and C'4 due to antigenic stimulation. Normal level of complement is established and compared to other workers as well as against guinea pig levels. Young rats were bled to establish normal levels of complement. The animals were then injected with an antigenic substance and after a period of seven days were bled again to determine the level of complement. Various antigenic and non-antigenic substances were used as well as normal saline injections for control.
Bacteriological Studies of the Campus Drinking Fountains of North Texas State Teachers College Denton, Texas
"In order to gain an adequate idea of the sanitary condition of the drinking fountains on the North Texas State Teachers College campus, it was found necessary to approach these bacteriological studies from a seasonal point of view."--1.
A Technique for the Mass Culture of Aquatic Actinomycetes
The purpose of the problem was to develop a laboratory technique for mass culturing of the aquatic actinomycetes. In order to solve the problem, it was necessary to devise a suitable culture chamber that would nurture the various species in both primary and secondary stages. It was also important to provide an adequate medium for growth of these organisms. Finally, the construction of the culture chambers must provide for a continuous exhaust of the taste and odor compounds so that the production of these components could be correlated with growth stages.
An Investigation of Naturally Occurring Tastes and Odors from Fresh Waters
The Problem of the cause and control of tastes and odors in water supplies has been the subject of a great many investigations during the last half-century; however, many questions in this field remain to be answered. Theories relating to the cause of tastes and odors occurring in water are still rather numerous and, at present, only a few of them are compatible. Although several procedures have been employed for the eradication of tastes and odors from water supplies, none of them are completely successful.
Effect of Immune Guinea Pig Serum and Cortisone on AKR Mouse Leukemia
This work is concerned with an attempt to clarify the role of cortisone in both the immune complement response and the progression of mouse leukemic tumor.
Isolation, Identification and Cultivation of Four Phytopathogenic Fungi
This investigation includes a morphological and cultural study of four rather common pathogenic fungi one of which attacks Zinnia elegans, one Lagerstroemia indica, one Ligustrum lodense and one Euonymus japonica. The problem includes, first, a determination of the genera of these four fungi as revealed by their morphology in natural habitat; second, a determination of the growth and cultural characteristics of these on Cornmeal, Potato, Bean, Prune and Carrot agars; third, a comparison of the growth and cultural characteristics of these in natural habitat with the same on the various agars used; and fourth, an evaluation of the five artificial culture media in so far as their usage is concerned in the growth and cultivation of these four species of fungi.
Isolation and Identification of Bacterial Species Associated with the Common House Fly and Three Species of Cockroaches
In an attempt to demonstrate the role of insects as possible carriers of pathogenic bacterial organisms this investigation has been concerned with the determination of the microbial population associated both internally and externally with the common house fly and three species of the cockroach.
The Antibiotic Activities of Some Members of the Cactaceae Family
This problem has been concerned with, first, the collection of sixteen species of plants belonging to Cactaceae family; second, the drying of these and the extraction of the oleoresins thereof; third, the determination of the extent to which these substances inhibit the growth of ten gram-positive and ten gram-negative bacterial organisms; and fourth, a determination of the possible utilization of these extracts a prophylactic or chemotherapeutic agents.
An Investigation of Chloral Hydrate as an Inhibitor of Bacterial Spreader Colonies in Milk Plate Counts
This study has consisted primarily of the addition of varying amounts of chloral hydrate to nutrient agar plates and the determination of the definite effects thereof upon the colony plate counts of various samples of milk.
A Microbial Survey of Raw Ingredients Used in Finished Products at Kraft Foods Company, Garland, Texas
The purpose of this investigation is to determine through routine checks the number of organisms present in the various raw ingredients used for the preparation of foods. The problem has consisted of, first, a determination of the total bacterial population by numbers; second, a determination of the incidence of colon bacteria in the samples examined; third, a determination of the presence of yeasts and molds; and fourth, an attempt to utilize this information obtained in judging the methods of handling the raw ingredients before they are used in the processing of foods.
The Application of Chlorine Dioxide to Tastes and Odors in Water Supplies
This problem was undertaken to determine the potentialities of chlorine dioxide as an algaecide and as an agent for the removal of tastes and odors from fresh water.
Extracts of Tree Leaves as Sources of Nutrition for Various Microorganisms
The purpose of this investigation is to determine the possible presence and the extent of nutritional material in the extracts of the green leaves of a selected group of common trees in an effort to devise simpler and more economical, yet useful and satisfactory, culture media for the use in bacteriological laboratories, particularly those on the secondary level.
The Chemical Compounds Produced by Actinomycetes and Their Relation to Tastes and Odors in a Water Supply
The purpose of this problem was to determine the cause of tastes and odors in the Breckenridge water supply and to isolate and culture the organisms responsible for the offensive chemicals.
An Investigation of Microbial Antagonism in Ten Denton County, Texas Soils
This thesis is concerned primarily with the growth and detection of soil microorganisms in ten Denton County, Texas, soils antagonistic to a specific gram-positive and a specific gram-negative bacterial organism.
Biological and Toxicological Responses Resulting from Dechlorination of a Major Municipal Wastewater Treatment Plant Discharge to the Trinity River
Federal regulations such as the Clean Water Act (P.L. 92-500), and its amendments, direct the Environment Protection Agency (EPA) to implement programs to control the releases of conventional pollutants and toxics into the waterways of the United States. The EPA began requiring treatment plants to conduct toxicity tests (biomonitoring) of their effluent discharges. To control toxicity caused by chlorination of wastewater discharges, the EPA also began requiring some treatment facilities to dechlorinate their wastewater before discharging. This research was funded by the EPA to document the changes that occurred in the Trinity River from the dechlorination of the effluent from Ft. Worth's Village Creek municipal wastewater treatment plant. The study occurred over a two year period beginning in August 1990. A wide variety of biological field assessments and toxicological assays were used to measure various responses. Seven river stations, covering approximately twenty river miles, and the treatment plant effluent were assessed. Two of the river stations were upstream from the treatment plant and used as reference sites. The remaining five river stations were downstream from the treatment plant, spread out over seventeen river miles. The study evaluated the impact of chlorination prior to dechlorination, which served as a baseline. Responses determined during dechlorination were compared to the baseline data. An overall improvement in species richness and diversity was seen at those river stations which had previously been adversely impacted by chlorine. Aquatic toxicity tests, such as those required to be used by dischargers, were conducted during this study. Periodic toxicity was observed with these tests in the effluent and river samples after dechlorination was initiated. Those tests, along with in situ toxicity assays, proved to be good predictors of biological community responses.
The Hypothermic Perfusion of the Isolated Thyroid Gland and Its Release of T₃ And T₄
Investigations have shown that the hypothalamus and pituitary respond to decreases in body temperature by stimulating thyroid release of T_3 and T_4 . This study was designed to bypass the control of the hypothalamus and pituitary gland and investigate the direct effect of temperature on the thyroid gland. Hypothermia was by an in vivo isolated perfusion of the thyroid gland. Radio-immunoassay was used to measure T_3 and T_4 concentrations. Significant increases were observed in animals perfused between 36º and 25ºc. These results indicate that the thyroid gland is directly effected by decreased temperature and that it is capable of exerting control over body temperature independent of the hypothalamus and pituitary gland. Lower perfusion temperatures produced no significant increases.
An Investigation of Selected Factors Related to the Professional Status of the Science Teachers in the Four-Year Accredited High Schools of Texas for the School Year 1952-1953
The purpose of this study is to gather information related to the professional status of the science teachers in the four-year accredited high schools of Texas for the school year 1952-1953. The principal concern of the study is "Who is teaching science?"
Extracts of Garden Vegetables as Sources of Nutrition for Various Microorganisms
This study was undertaken in order to determine whether the extracts of common garden vegetables could be incorporated into simple, economical culture media which might be used for the growth and cultivation of at least some of the more commonly used microorganisms.
Biosystematic Study of a Desmodium Complex
An examination of the Desmodium canescens complex (D. canescens; D. tweedyi; D. illinoense) has resulted in the delimitation of a previously unreported alliance between D. canescens and D. tweedyi. The following points support this view: (a) morphological data taken from herbarium and garden specimens indicate that for many characters, the mean values of D. canescens and D. tweedy are not significantly different (b) breeding experiments have shown that artificial interspecific hybridization is possible between D. canescens and D. tweedyi (c) cytological studies have shown that D. canescens and D. tweedyi have a base number of x = 11, while D. illinoense has a base number of x = 10. A new combination is suggested: Desmodium canescens var. tweedyi (Britt.) Williams.
Herpetology of the Amistad Reservoir Area, Val Verde County, Texas
The Amistad Reservoir in southern Val Verde County, Texas will impound a considerable amount of surface water in a semi-arid region in which natural surface water is scarce. Construction of the dam began in 1963 and the reservoir began to fill in the summer of 1968. This large impoundment could have a considerable ecological impact on the area. The purpose of this research was to study the ecological distribution and zoogeographic affinities of the herpetofauna and to establish an ecological baseline against which any future changes could be measured.
The Population Dynamics and Trophic Relationships of Seven Species of Fish in a Small Southwestern Pond, with Special Attention Toward Young-of-the-Year Fish
Production rates of seven species of fish, along with food-web interactions, were determined for one year in a pond. Production of fish in the pond amounted to 208.9 q/m2/yr. Over 95% of the production occurred in the summer, with Y of Y fish contributing 83.8%. There were two food-web interactions observed in Y of Y fishes: crappie vs. bass and bullhead vs. sunfish. When Y of Y fish shifted to benthic diets in the spring, the small biomass of benthos available could not sustain the larger biomass of fish. Competition for the available food resulted in decreased production rates for the fish community.
Aryl Hydrocarbon Hydroxylase and Sixteen Alpha Hydroxylase in Cultured Human Lymphocytes
Cultured human lymphocytes may be assayed for aryl hydrocarbon hydroxylase (AHH) in whole cell preparations. The optimum assay conditions are pH 8.5, and 1.5 mM Mg++. The reaction is linear with time and cell number, and is inhibited by CO. Estradiol may inhibit induction of AHH by 3-methylcholanthrene, but is a poor competitor for the enzyme. A Caucasian population was assayed for AHH activity. The distribution was lognormal; no difference was found in cultured cells from males and females or smokers and nonsmokers. Cells from relatives of lung cancer patients showed higher activity. An American Indian population showed no difference from the Caucasian population in enzyme level. No linkage was found between AHH and 16a-hydroxylase.
Induction of 16α Hydroxylase in Human Cultured Lymphocytes
A method is presented for 160hydroxylase (SAH) induction in cultured human lymphocytes. SAH, a microsomal-associated enzyme, effects the oxidative conversion of 17pestradiol to estriol, which competes for cytoplasmic binding sites. 17,-estradiol and estrone are known mammary carcinogens, while estriol and its epimers have been suggested to have anticarcinogenic properties. To substantiate genetic variations of hydroxylase activity, an analysis of estrogen-induced cultured human lymphocytes was conducted to evaluate the frequency distribution of low, intermediate, and high SAH activity. Frequency analysis indicated that the control population distribution of SAH activity does not corroborate a proposed trimodal expansion of human SAH activity. A log normal distribution of SAH activity does exist, which suggests a polygenic mode of genetic control. SAH activity in a population of breast cancer patients and relatives of breast cancer patients showed no statistical difference from the SAH activity in the control population.
Differences in Protein Constituents of Some Azotobacter Species
This study used polyacrylamide gel electrophoresis to study the acid-phenol soluble proteins of five strains (A. vinelandii 12837, A. vinelandii 0, A. chroococcum 8004, A. macrocytogenes 8702, A. tumefaciens) of bacteria grown on Burk's nitrogen-free media, Trypticase Soy Broth, and 0.3% butanol medium. The results showed that the protein patterns can be used for the identification and possibly the taxonomic classification of the Azotobacter. The change of phenotype of the bacteria in different media followed the change of protein quantity and quality. There was no absolute similarity between any two of the species studied and this suggests a genetically heterogenous group of organisms while the amount of common proteins suggests close genetic relationships. Further studies are necessary to confirm the status of A. tumefaciens.