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Genetic Modification of Fatty Acid Profiles in Cotton

Description: The industrial uses of cottonseed oil are limited by its fatty acid composition. Genetic modification of cotton lipid profiles using seed-specific promoters could allow cotton growers to produce valuable new oils in the seed without adverse effects on fiber quality and yield, therefore making this crop more commercially profitable. Transgenic cotton callus harboring a diverged fatty acid desaturase gene (FADX) from Momordica charantia was characterized for production of alpha-eleostearic acid (conjugated double bonds: 18:3 D9 cis, 11 trans, 13 trans), not normally found in cotton. Gas chromatography (GC) in conjunction with mass spectrometry (MS) confirmed production of alpha-eleostearic acid in the transgenic cotton tissues. A second series of transformation experiments introduced the cotton fatty acid thioesterase B (FATB) cDNA, fused to the seed-specific oleosin promoter into cotton to promote the over-expression of FATB, to generate cotton with increased palmitate in the cottonseed. PCR amplification, as well as fatty acid analysis by gas chromatography, confirmed introduction of the FATB cDNA in transgenic tissues. Collectively, these results demonstrate the feasibility of manipulating the fatty acid composition in cotton via transgenic approaches and form the basis for continued efforts to create novel oils in cottonseed.
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Date: August 2005
Creator: Rommel, Amy A.

A geospatial tool for assessing potential wildland fire risk in central Texas.

Description: Wildland fires in the United States are not always confined to wilderness areas. The growth of population centers and housing developments in wilderness areas has blurred the boundaries between rural and urban. This merger of human development and natural landscape is known in the wildland fire community as the wildland urban interface or WUI, and it is within this interface that many wildland fires increasingly occur. As wildland fire intrusions in the WUI increase so too does the need for tools to assess potential impact to valuable assets contained within the interface. This study presents a methodology that combines real-time weather data, a wildland fire behavior model, satellite remote sensing and geospatial data in a geographic information system to assess potential risk to human developments and natural resources within the Austin metropolitan area and surrounding ten counties of central, Texas. The methodology uses readily available digital databases and satellite images within Texas, in combination with an industry standard fire behavior model to assist emergency and natural resource managers assess potential impacts from wildland fire. Results of the study will promote prevention of WUI fire disasters, facilitate watershed and habitat protection, and help direct efforts in post wildland fire mitigation and restoration.
Date: August 2005
Creator: Hunter, Bruce Allan

Global response to cyberterrorism and cybercrime: A matrix for international cooperation and vulnerability assessment.

Description: Cyberterrorism and cybercrime present new challenges for law enforcement and policy makers. Due to its transnational nature, a real and sound response to such a threat requires international cooperation involving participation of all concerned parties in the international community. However, vulnerability emerges from increased reliance on technology, lack of legal measures, and lack of cooperation at the national and international level represents real obstacle toward effective response to these threats. In sum, lack of global consensus in terms of responding to cyberterrorism and cybercrime is the general problem. Terrorists and cyber criminals will exploit vulnerabilities, including technical, legal, political, and cultural. Such a broad range of vulnerabilities can be dealt with by comprehensive cooperation which requires efforts both at the national and international level. "Vulnerability-Comprehensive Cooperation-Freedom Scale" or "Ozeren Scale" identified variables that constructed the scale based on the expert opinions. Also, the study presented typology of cyberterrorism, which involves three general classifications of cyberterrorism; Disruptive and destructive information attacks, Facilitation of technology to support the ideology, and Communication, Fund raising, Recruitment, Propaganda (C-F-R-P). Such a typology is expected to help those who are in a position of decision-making and investigating activities as well as academicians in the area of terrorism. The matrix for international cooperation and vulnerability assessment is expected to be used as a model for global response to cyberterrorism and cybercrime.
Date: August 2005
Creator: Ozeren, Suleyman

Government Funding and Regulation of a Texas Voucher Program

Description: This study was designed to determine how willing private schools are to participate in a limited school voucher program if various state regulations are required and whether willingness to participate varies among types of schools. Provisions of voucher bills proposed in the Texas legislature and requirements included in other states' legislation were used to determine the sample, hypothetical voucher amount, and possible state regulations. Three hundred eighteen surveys were sent, and 150 were returned, giving a 47% return rate. Data were entered into SPSS and analyzed using chi-square and crosstabs. Initially chi square was used to see if findings were significant at the .0041 level. This alpha level was reached by using the Bonferroni correction factor, which holds experiment wise Type I error to .05. Crosstabs was used to determine if relationships between regulation acceptance and type of schools were significant. Overall, as the amount of regulation increased, private school willingness to participate in a voucher program decreased. The regulations rejected by a large majority of schools in all categories were open admissions and student religious exemptions. In the areas of testing, curriculum, and teacher qualifications, private schools were much more willing to participate if they were allowed to utilize their own practices than if required to follow regulations required of Texas public schools. These findings were significant. When analyzing what type of school would be most interested in participating in a voucher program, the factor that yielded the most significant results was amount of yearly tuition. Private school willingness to participate in a voucher program was directly related to the amount of tuition charged. Those private schools with tuitions that were at or below the hypothetical voucher amount were much more likely to participate than those with higher tuition. Overall, significant results were shown with almost every regulation.
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Date: August 2005
Creator: Morgan, Lisa Ann

Group sandtray therapy at school with preadolescents identified with behavioral difficulties.

Description: Sandtray therapy, a modality of play therapy, has been used in a variety of ways as the treatment intervention with different theoretical approaches; however, there is a very limited amount of empirical research. The purpose of this research is to examine the effectiveness of group sandtray therapy at school with preadolescents identified with behavioral difficulties. This is a pretest-posttest control group design. Participants in the experimental group received sandtray therapy in group for ten weeks, and participants in the wait-list control group received no treatment intervention. The researcher compared two groups to examine the overall effectiveness of sandtray therapy as determined by the scores of the Child Behavior Checklist-Teacher Report Form (CBC-TRF), Parent Report Form (BASC-PRF), and Self Report of Personality (BASC-SRP). Based on teachers' reports, statistically significant difference existed between the two groups in terms of preadolescents' overall behaviors, externalizing behavior problems, and internalizing behavior problems after the ten week treatment intervention. The effect sizes were medium (d= .52-.59). According to parents' reports, a statistically significant difference was found regarding preadolescents' externalizing behavior problems, and the effect size was medium (d=.63). No statistically significant differences were found regarding preadolescents' total behaviors and internalizing behavior problems based on BASC-PRF. The effect sizes arranged from medium to small (d=.55 and .35, respectively). In terms of the total behavior on BASE-SRP, no statistical significant difference was found and the effect was small (d=.18). A case example was included to illustrate the process and effect of group sandtray therapy. Based on the results of this study, it is determined that group sandtray can be an effective treatment intervention for preadolescents identified with behavioral problems. The primary contribution of this study is to present empirical support for the effectiveness of using sandtray therapy.
Date: August 2005
Creator: Flahive, Mon-hsin Wang

Hemispheres for Wind Ensemble by Joseph Turrin: A Critical Analysis

Description: Hemispheres is a three-movement work for winds written by Joseph Turrin in May 2002. Commissioned by Kurt Masur for the New York Philharmonic, he wished to include a piece exclusively for winds and percussion in the programming of his farewell concert that commemorated his eleven years as Music Director. The work is in three movements: Genesis, Earth Canto, and Rajas which represent three different cultural views of creation. Formally, this work is based structurally and thematically on melody rather than harmony. This analysis focuses on three main tools which unify this work. The first is that thematic material from the first movement is reintroduced and developed in the second and third movements. The second is a consistently reoccurring rhythmic grouping in threes. This three note motive, found in all three movements, is used both melodically and as an accompaniment. The third is the unifying pitch center of C. Through an economy of musical means, Turrin composed Hemispheres with only a minimal number of themes and motives, each developed through the course of all three movements.
Date: August 2005
Creator: deAlbuquerque, Joan

A Historical Analysis of the Failures of Camp David 2000 Summit

Description: This research seeks to understand the reasons for failures of Bill Clinton, Yasser Arafat, and Ehud Barak's Camp David Summit that was held in July, 2000. The Summit was arranged to complete the last phase of Oslo Peace Process. Numerous researches have attempted to reveal the facts of the summit but, so far, they have failed to present the complete details of what happened before, during, and after the summit. This research explores all aspects of the problem including the various variables that would have had effected the breakdown of the Middle East peace process. Finally, the researcher determines the parameters needed to maintain a substantial peace in the Middle East and what proposed strategies might be followed in order to avoid the previous mistakes in future peace negotiations.
Date: August 2005
Creator: Yilmaz, Ismail

Holocaust Song Literature: Expressing the Human Experiences and Emotions of the Holocaust through Song Literature, Focusing on Song Literature of Hirsh Glick, Mordechai Gebirtig, and Simon Sargon

Description: During the years of the Holocaust, song literature was needed to fulfill the unique needs of people caught in an unimaginable nightmare. The twelve years between 1933 and 1945 were filled with a brutal display of man's inhumanity to man. Despite the horrific conditions or perhaps because of them, the Jewish people made music, and in particular, they sang. Whether built on a new or an old melody, the Holocaust song literature continues to speak to those of us who are willing to listen. This body of work tells the world that these people lived, suffered, longed for vengeance, loved, dreamed, prayed, and tragically, died. This repertoire of songs is part of the legacy, the very soul of the Jewish people. This study contains a brief look at the historical circumstances, and through the song literature of Hirsh Glick, Mordechai Gebirtig and Simon Sargon, life within the ghetto, the concentration camp, the decisions families had to make, the choices to fight back against incredible odds, the place of faith within this nightmare, and a look at the lives and works of the composers themselves.
Date: August 2005
Creator: Nedvin, Brian

Iconoclast in the mirror.

Description: This work explores identity positions of speakers in modern and contemporary poetry with respect to themes of subjectivity, self-awareness, lyricism, heteroglossia, and social contextualization, from perspectives including Bakhtinian, queer, feminist and postructuralist theories, and Peircian semiotics. Tony Hoagland, W.H. Auden, Adrienne Rich, and the poetic prose of Hélène Cixous provide textual examples of an evolving aesthetic in which the poet's self and world comprise multiple dynamic, open relationships supplanting one in which simple correspondences between signifiers and signifieds define selves isolated from the world. Hypertext and polyamory serve as useful analogies to the semantic eros characteristic of such poetry, including the collection of original poems that the critical portion of this thesis introduces.
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Date: August 2005
Creator: Alexander, Lydia L.

Identity development across the lifespan.

Description: In an extension of Louden's work, this study investigated identity development across the lifespan by applying Erickson's and Marcia's identity constructs to two developmental models, the selective optimization and compensation model and a holistic wellness model. Data was gathered from traditionally aged college freshmen and adults older than 60 years of age. Uncommitted identity statuses and work and leisure wellness domains were endorsed across both groups, suggesting that identity for these groups is in a state of fluctuation yet entailing participation as a productive member of society. Emerging adult findings imply that identity diffused and moratorium identity styles are more similar in terms of cognitive, behavioral, and emotional functioning than past literature suggests for this age group. Findings also indicate that identity development is not a process completed by older adulthood, but is an ongoing, lifelong process perhaps driven by contextual factors such as health changes, unpredictable life events, social support group changes, and others. Coping method utilization and overall wellness varied between the two age groups. Conceptually, the SOC model can be viewed as embedded within each of the wellness domains such that selection, optimization, and compensation activities may be carried out within each of the various domains and serve to enhance existing functioning within each domain rather than simply compensating for lost functioning. Possible explanations of the results as well as implications for clinical practice, higher education, and future research are provided.
Date: August 2005
Creator: Louden, Linda L.

The impact of rising women's salaries on marital and relationship satisfaction.

Description: Using data from a national survey, this study examines income and other key variables (division of labor and work-family conflict) and their relationship to marital satisfaction. This study builds upon the body of research regarding working couples and women's increased participation in the paid labor force as well as evaluates the findings in the context of data gathered from the recent United States census. Results from this study also are compared to the findings of other key studies. Emergent data may be used to prepare counselors to work more effectively with couple clients and to assist employers in the development of work life policies for dual career and dual earner employees. Results from the multiple regression revealed no direct effects of income on marital satisfaction. For this sample, increases in work family conflict contributed to less marital satisfaction as did the presence of children. Increased participation in household chores by respondents' partners contributed to increased marital satisfaction. No differences were observed by gender. Limitations of the study, recommendations for further research, and implications for practitioners also are addressed.
Date: August 2005
Creator: Menninger, Sarah Wheeler

The Improvisational Vocabulary of Pepper Adams: A Comparison of the Relationship of Selected Motives to Harmony in Four Improvised Solos

Description: Park "Pepper" Adams, III (1930-1986) is one of the most influential baritone saxophonists in the history of modern jazz. In addition to his time feel, his timbre, and other conceptual techniques, a great deal of Adams's improvisational style and vocabulary can be illustrated by his use of three motivic devices. These three motivic devices are: (1) his utilization of the sixth degree of the major scale as an important melodic pitch; (2) his use of a paraphrased portion of the melody of the popular song "Cry Me a River;" and (3) his use of the half-whole octatonic scale when the rhythm section sounds a dominant chord. This dissertation traces the way in which Adams applies these three motivic devices through four of his original compositions, "Enchilada Baby," "Bossallegro," "Lovers of Their Time," and "Rue Serpente." All four of these compositions were recorded by Adams on his 1980 album, The Master. In addition to the motivic analysis, a biography of Adams is included. Complete transcriptions by the author of Adams's improvised solos on the four compositions are included in the appendices.
Date: August 2005
Creator: Lington, Aaron Joseph

International police cooperation as a response to transnational organized crime in Europe: Improvements in extradition.

Description: International criminality has been a challenging phenomenon for national police forces for years. States have developed international police cooperation relations and extradition instruments in order to fight international criminal activity. This treatise explores the reasons for the rise in transnational organized crime activities in Europe and presents an in-depth explanation concerning the emergence, mandates, and structures of multilateral police collaboration systems such as Interpol, Trevi, Schengen, and Europol. Since the extradition has become an inseparable part of international policing, this study examines the improvements in extradition procedure and emphasizes the importance of extradition. Finally this study compares traditional (European Convention on Extradition of 1957) and new (European Arrest Warrant) extradition systems.
Date: August 2005
Creator: Durmaz, Huseyin

An Investigation of the Impact of Technology Expenditures on Student Achievement in Texas Districts

Description: The purpose of this study was to explore the relationship between money spent on technology hardware, software, and training on district-wide achievement as measured by Texas standardized achievement tests, the Scholastic Aptitude Test (SAT), and the American College Test (ACT). A series of studies were carried out to develop a model of the relationship between Texas district TAKS, TAAS, ACT, and SAT scores for all subjects and district expenditures on technology hardware, technology software, and technology training. The findings of this study showed that although the mixture of uneven distribution of training, incentives, and equipment in these Texas districts clouds the issue of effective integration as it does for all districts (Anderson & Becker, 1998), and the mean level of per pupil technology expenditure for participating districts is of an amount ($192 per student) deemed unlikely to have substantial impact on student outcomes (Anderson & Becker, 1998), there are strong positive links between levels of expenditure and student achievement on the Texas Assessment of Knowledge and Skills and the American College Test that indicate that establishing guidelines for levels of expenditure, schedules of acquisition of materials and equipment, and timeframes for training and implementation may be vital to the success of technology integration in these districts and potentially for all districts in the nation. More study into effective funding levels, schedules of acquisition of materials and equipment, and timeframes of implementation is necessary to create truly successful programs of technology integration in school districts.
Date: August 2005
Creator: Hancock, Robert

Lawrence Weiner's Concerto for Guitar and Orchestra: Analysis and Performance Considerations

Description: The purpose of this dissertation is to provide an introduction to the composer, Lawrence Weiner, and to his Concerto for Guitar and Orchestra, one of the many neglected guitar concerti that merits closer study and more frequent performances. Weiner, a prolific and prominent composer in the South Texas region, composed the concerto in 1986. The concerto was never published. This dissertation is the first attempt to study Weiner's Concerto for Guitar and Orchestra to date. This study provides insight into the compositional style of Weiner, and the understanding and performing of this work. The compositional language of this concerto is examined through an analysis of the aspects of form, harmonic/tonal scheme and thematic development. A performance edition and suggestions that are approved by the composer are also presented.
Date: August 2005
Creator: Tan, Wann-Dar

Makeshift Information Constructions: Information Flow and Undercover Police

Description: This dissertation presents the social virtual interface (SVI) model, which was born out of a need to develop a viable model of the complex interactions, information flow and information seeking behaviors among undercover officers. The SVI model was created from a combination of various philosophies and models in the literature of information seeking, communication and philosophy. The questions this research paper answers are as follows: 1. Can we make use of models and concepts familiar to or drawn from Information Science to construct a model of undercover police work that effectively represents the large number of entities and relationships? and 2. Will undercover police officers recognize this model as realistic? This study used a descriptive qualitative research method to examine the research questions. An online survey and hard copy survey were distributed to police officers who had worked in an undercover capacity. In addition groups of officers were interviewed about their opinion of the SVI model. The data gathered was analyzed and the model was validated by the results of the survey and interviews.
Date: August 2005
Creator: Aksakal, Baris

Marital Satisfaction and Stability Following a Near-Death Experience of One of the Marital Partners

Description: The purpose of this quantitative and qualitative study was to determine retrospectively marital satisfaction and stability following the near-death experience (NDE) of one of the marital partners, focusing on the role of Gottman's Sound Marital House (1999) in the couple's relationship before and after the NDE. The researcher used the Locke-Wallace Marital Adjustment Test (1959), the Weiss-Ceretto Marital Status Inventory (1980), and a modification of Gottman's Shared Meanings Questionnaire (1999). The first group of participants included 26 NDErs. To create as comparable a group as possible, the researcher designed a life-changing event (LCE) group of 26 people who used as their referent the non-NDE-related experience they considered their most life-changing one during their marriage. Sixty-five percent of the marriages in which the NDErs were involved at the time of their NDEs ended in divorce. This number is in contrast to the 19 percent of LCE participants whose marriages ended in divorce. Marital adjustment, marital stability, and meanings in marriage between retrospectively based pre-event and post-event composite scores were statistically significantly different between the NDErs and LCErs. Low effect sizes were identified for each of the instruments except the Weiss-Ceretto Marital Status Inventory, which had a moderate effect size. Strong correlations among the scores were identified. Further analysis of the results indicated strongly that the NDErs were less satisfied in their marriages, their marriages were less stable, and they did not have a strong level of shared meaning in the marriage after the NDE occurred as compared to the LCE participants. This study has serious implications for counselors who may work with NDErs. Findings from this study show that NDErs who were married at the time of their experiences have a strong possibility of experiencing marital problems. Encouraging these couples to seek professional help as soon as possible can provide a ...
Date: August 2005
Creator: Christian, Sandra Rozan

Measuring Team Meeting Success: Does Everyone Really Need to Participate?

Description: Facilitators are encouraged to get all meeting attendees to participate in the meeting. There is the assumption made that, if they do participate, then this participation will increase the group's general satisfaction of the meeting. Also, knowing the factors that can increase the probability of a successful meeting has been a focus of previous research, yet attendee participation has not been studied. The current research study empirically examines participation's effect on meeting evaluations. This study is a field experiment conducted in a team-based organization, where successful meetings are critical. Data was collected on the amount of participation of team members in their weekly team meeting and their evaluations of the meeting. After running correlations and a principal components analysis, this study found a relationship between participation and meeting evaluations. A scale of meeting success was also created.
Date: August 2005
Creator: Longo, Jodi Olaine

Meta-Analysis of Reading Interventions for Students with Learning and Emotional Disabilities

Description: Developing effective literacy skill has become an increasingly critical skill in today's information age. Students with emotional/behavioral disorders (E/BD) routinely lack these skills and are not being taught how to read effectively. The field of special education needs more comprehensive and specific information about how to most effectively teach reading skills to students with E/BD. When reading interventions are conducted using student with E/BD, the interventions are generally drawn from the LD field. The assumption is that the reading interventions that have worked with students with LD will work equally well with the E/BD population. This study performed a meta-analysis to examine whether reading interventions are equally effective on the E/BD and LD populations. In addition, it will examine whether the instruction mode (e. g., peer, self, or teacher directed), gender, or grade group affects the success of the intervention. The meta-analysis found that the reading interventions for both disability groups had high effect sizes. In addition, neither disability group, teaching method, gender, nor grades were predictive of the variance in the effect size. These results indicate that reading programs that have been designed for students with LD are also effective for students with E/BD and furthermore, reading programs can improve the academic achievement of students with behavioral disorders. Recommendations for teacher training and future research are given based on these results.
Date: August 2005
Creator: Jones, Francesca

Metal Oxide Reactions in Complex Environments: High Electric Fields and Pressures above Ultrahigh Vacuum

Description: Metal oxide reactions at metal oxide surfaces or at metal-metal oxide interfaces are of exceptional significance in areas such as catalysis, micro- and nanoelectronics, chemical sensors, and catalysis. Such reactions are frequently complicated by the presence of high electric fields and/or H2O-containing environments. The focus of this research was to understand (1) the iron oxide growth mechanism on Fe(111) at 300 K and 500 K together with the effect of high electric fields on these iron oxide films, and (2) the growth of alumina films on two faces of Ni3Al single crystal and the interaction of the resulting films with water vapor under non-UHV conditions. These studies were conducted with AES, LEED, and STM. XPS was also employed in the second study. Oxidation of Fe(111) at 300 K resulted in the formation of Fe2O3 and Fe3O4. The substrate is uniformly covered with an oxide film with relatively small oxide islands, i.e. 5-15 nm in width. At 500 K, Fe3O4 is the predominant oxide phase formed, and the growth of oxide is not uniform, but occurs as large islands (100 - 300 nm in width) interspersed with patches of uncovered substrate. Under the stress of STM induced high electric fields, dielectric breakdown of the iron oxide films formed at 300 K occurs at a critical bias voltage of 3.8 ± 0.5 V at varying field strengths. No reproducible result was obtained from the high field stress studies of the iron oxide formed at 500 K. Ni3Al(110) and Ni3Al(111) were oxidized at 900 K and 300 K, respectively. Annealing at 1100 K was required to order the alumina films in both cases. The results demonstrate that the structure of the 7 Å alumina films on Ni3Al(110) is k-like, which is in good agreement with the DFT calculations. Al2O3/Ni3Al(111) (γ'-phase) and Al2O3/Ni3Al(110) (κ-phase) ...
Date: August 2005
Creator: Qin, Feili

Morphological properties of poly (ethylene terephthalate) (PET) nanocomposites in relation to fracture toughness.

Description: The effect of incorporation of montmorillonite layered silicate (MLS) on poly (ethylene terephthalate) (PET) matrix was investigated. MLS was added in varying concentration of 1 to 5 weight percent in the PET matrix. DSC and polarized optical microscopy were used to determine the crystallization effects of MLS addition. Non isothermal crystallization kinetics showed that the melting temperature and crystallization temperature decrease as the MLS percent increases. This delayed crystallization along with the irregular spherulitic shape indicates hindered crystallization in the presence of MLS platelets. The influence of this morphology was related with the fracture toughness of PET nanocomposites using essential work of fracture coupled with the infra red (IR) thermography. Both the essential as well as non essential work of fracture decreased on addition of MLS with nanocomposite showing reduced toughness.
Date: August 2005
Creator: Pendse, Siddhi

Mouse cortical cholinergic neurons: Ontogeny of phenotypes in vivo and in vitro.

Description: The development of cholinergic neurons in mouse frontal cortex was studied both in vivo and in vitro by immunocytochemistry with an antibody to choline acetyltransferase (ChAT), the enzyme responsible for acetylcholine synthesis. While cortical cholinergic neurons have previously been characterized in rat cortex, up until very recently, intrinsic cortical cholinergic neurons were considered to be absent in mouse, and little is known about their development or phenotypic characteristics. The present study found no ChAT-positive neurons in mouse frontal cortex on postnatal day 0 (P0, the day of birth). On P7 there were few, faintly stained, ChAT-positive neurons. The numerical density of ChAT-positive neurons increased substantially with age, from none on P0, to 9.2 + 1.4 on P7, to 14.8 + 0.9 on P16, and 41.6 + 3.9 in adulthood. Considering that the numerical density of total neurons decreases during this postnatal period, the data represent a marked developmental increase in the percentage of cholinergic neurons. The development of cholinergic neurons showed very similar timelines in rat and mouse frontal cortex. Cultures prepared from mouse frontal cortex on embryonic day 16 were maintained for 25, 76, or 100 days in vitro (div). The percentage of ChAT-positive neurons was considerably higher than in vivo, ranging from a mean 28% to 31% across the three age (div) groups. With increasing age of the cultures, the numerical density of total neurons and ChAT-positive neurons decreased while the percentage of ChAT-positive neurons did not change significantly. These observations suggest some temporal stability in the cultures. Using dual immunofluorescence, ChAT-positive neurons were tested for colocalization with GAD or TH. The majority of ChAT-positive neurons colocalized with GAD, both in vitro and in vivo. However, ChAT did not colocalize with TH, either in vitro or in vivo. Our comparison of intact frontal cortex and cultures suggest that ...
Date: August 2005
Creator: Coiculescu, Olivia Elena

Mutation of Polaris, an Intraflagellar Transport Protein, Shortens Neuronal Cilia

Description: Primary cilia are non-motile organelles having 9+0 microtubules that project from the basal body of the cell. While the main purpose of motile cilia in mammalian cells is to move fluid or mucus over the cell surface, the purpose of primary cilia has remained elusive for the most part. Primary cilia are shortened in the kidney tubules of Tg737orpk mice, which have polycystic kidney disease due to ciliary defects. The product of the Tg737 gene is polaris, which is directly involved in a microtubule-dependent transport process called intraflagellar transport (IFT). In order to determine the importance of polaris in the development of neuronal cilia, cilium length and numerical density of cilia were quantitatively assessed in six different brain regions on postnatal days 14 and 31 in Tg737orpk mutant and wildtype mice. Our results indicate that the polaris mutation leads to shortening of cilia as well as decreased percentage of ciliated neurons in all brain regions that were quantitatively assessed. Maintainance of cilia was especially affected in the ventromedial nucleus of the hypothalamus. Furthermore, the polaris mutation curtailed cilium length more severely on postnatal day 31 than postnatal day 14. These data suggests that even after ciliogenesis, intraflagellar transport is necessary in order to maintain neuronal cilia. Regional heterogeneity in the effect of this gene mutation on neuronal cilia suggests that the functions of some brain regions might be more compromised than others.
Date: August 2005
Creator: Mahato, Deependra

"My Vagina" and other stories.

Description: This thesis includes seven short stories and a critical afterword. The afterword places the stories in their literary historical context in regards to creative nonfiction. It goes on to discuss the craft of fictionalizing autobiographical stories. Each of the stories should stand alone, though they follow the narrator's life for a number of years. Harlin Anderson is the narrator of all the stories.
Date: August 2005
Creator: Anderson, Aaron W.