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The Acceptance and Use of Cloud Computing Services by Small and Medium Enterprises in Lagos, Nigeria
This study explored the acceptance of cloud computing (CC) services by small and medium enterprises (SMEs) in Lagos, Nigeria, which has been missing from CC services literature. It aimed to understand the motivations for adoption, the uses of the services, and the benefits they derive from it. The uses and gratification theory was applied as the theoretic framework for this endeavor. An online survey with close-ended and open-ended questions was distributed to 1200 randomly selected participants through email. In total, 392 valid responses were collected and analyzed using descriptive statistics and categories. The results found that SMEs in Lagos, Nigeria had a low level of awareness and appreciation of CC services. The adoption rate was also low. Unlike their counterparts in other regions, SMEs primary concerns were service downtime, stable power supply, and better internet access. The study found that SMEs were not taking full advantage of the capabilities of CC services. Some sections, however, were doing better than others, such as the information and communications sub-sector. This study suggested that targeted interventions should be conducted to raise the awareness of CC services in SMEs, and to improve their efficient and effective use of CC services. The uses and gratification theory was appropriate for guiding this study to understand the acceptance and use of CC services by SMEs in Lagos, Nigeria.
Adequacy and Equity: How the Texas Supreme Court's Perceptions Have Changed Over the Past 50 Years
The purpose of this study identifies state court cases involving public school finance specifically related to adequacy and equity in funding. Results address how state court cases have challenged the constitutionality of school finance in the United States, including Texas, over the last 50 years. The study further shows how the decisions from previous cases have influenced the Supreme Court of Texas decision in the Texas Taxpayer & Student Fairness litigation.
Adrenergic and Cholinergic Regulation of Cardiovascular Function in Embryonic Neotropic Cormorants (Phalacrocorax basilianus)
Investigations of cholinergic and adrenergic tone on heart rate (fH) and mean arterial pressure (Pm) during embryonic development have been conducted on numerous avian species. While these investigations have documented that adrenergic tone, a continuous stimulation, on fH and Pm is vital to embryonic development in the birds studied to date, development of cholinergic tone on fH has been shown to vary even within species. Further, past studies have been bias to focus primarily on precocial species while altricial species remain poorly understood in this context. The goal of this investigation was to investigate the role of cholinergic and adrenergic tone on fH and Pm of an altricial species, the neotropic cormorant (P. brasilianus) to address this bias. The embryonic neotropic cormorant possesses B-and-a adrenergic tone on fH and Pm at 70% and 90% incubation while cholinergic tone on fH occurs at 90% incubation. This pattern of control is similar to that previously reported for several species of precocial birds suggesting the development of tonic cardiovascular regulation may be conserved across avian taxa.
Age Friendly Cities: The Bureaucratic Responsiveness Effects on Age Friendly Policy Adoption
Challenging a long-held attachment to the medical model, this research develops a cultural model placing local governments at the center of policy making and refocusing policy attention on mobility, housing, the built environment and services. To examine the phenomenon of age friendly policy adoption by cities and the magnitude of adoption, a 21-question web-based survey was administered to a sample of 1,050 cities from the U.S. Census having a population over 10,000 and having at least 14% of their population aged 65 years and over. The goal of the questionnaire was to help identify what kind of policy objectives cities establish to facilitate the opportunity for older adults to live healthy and independent lives in their communities as they age. Multiple linear and ordinal regression models examined the likelihood of policy action by cities and provide evidence as to why some cities support more age friendly policy actions than others. Evidence illustrates theoretical advancement providing support for a cultural model of aging. The cultural model includes multiple factors including bureaucratic responsiveness reflected in the management values of the administration. Findings show variation in the integration of a cultural awareness of aging in the municipality's needs assessment, strategic goals, citizen engagement strategies, and budgetary principles. Cities with a cultural awareness of aging are more likely to adopt age friendly policies. Findings also provide support for the argument that the public administrator is not the driving sole factor in decision making. A shared spaced with mobilized citizen need of individuals 65 and over is identified.
An Analysis of Litigation against Kansas Educators and School Districts under the Kansas Tort Claims Act
This dissertation examines the significance of the Kansas Tort Claims Act of 1979 on state of Kansas court decisions in litigation against Kansas school districts and their employees. Through providing a historical perspective of the adoption and abolishment of the doctrine of sovereign immunity in the United States, which subsequently led to the enactment of the Federal Tort Claims Act, and ultimately led to the Kansas Tort Claims Act, the researcher analyzes pertinent case law and scholarly commentary pertaining to school negligence litigation. The goal of the analysis is to answer the following research question: How have Kansas state courts interpreted the Kansas Tort Claims Act in litigation against state school districts and their employees? Although the KTCA provides citizens with a vehicle for redress against governmental entities by virtue of tort claims, the KTCA also provides immunities from liability for governmental entities and their employees under exceptions to the KTCA. Most notably, the discretionary function exception and the recreational use exception are two exceptions to liability applied in a significant number of tort cases against Kansas school districts and employees. The case law analysis provides explanations for the types of actions of negligence that Kansas courts have qualified for school district or school employee liability, and, when permitted, negligent actions that qualified for immunity under a KTCA exception to liability.
Application of High Entropy Alloys in Stent Implants
High entropy alloys (HEAs) are alloys with five or more principal elements. Due to these distinct concept of alloying, the HEA exhibits unique and superior properties. The outstanding properties of HEA includes higher strength/hardness, superior wear resistance, high temperature stability, higher fatigue life, good corrosion and oxidation resistance. Such characteristics of HEA has been significant interest leading to researches on these emerging field. Even though many works are done to understand the characteristic of these HEAs, very few works are made on how the HEAs can be applied for commercial uses. This work discusses the application of High entropy alloys in biomedical applications. The coronary heart disease, the leading cause of death in the United States kills more than 350,000 persons/year and it costs $108.9 billion for the nation each year in spite of significant advancements in medical care and public awareness. A cardiovascular disease affects heart or blood vessels (arteries, veins and capillaries) or both by blocking the blood flow. As a surgical interventions, stent implants are deployed to cure or ameliorate the disease. However, the high failure rate of stents has lead researchers to give special attention towards analyzing stent structure, materials and characteristics. Many works related to alternate material and/or design are carried out in recent time. This paper discusses the feasibility of CoCrFeNiMn and Al0.1CoCrFeNi HEAs in stent implant application. This work is based on the speculation that CoCrFeNiMn and Al0.1CoCrFeNi HEAs are biocompatible material. These HEAs are characterized to determine the microstructure and mechanical properties. Computational modeling and analysis were carried out on stent implant by applying CoCrFeNiMn and Al0.1CoCrFeNi HEAs as material to understand the structural behavior.
Applied Use of Video Modeling in Educational and Clinical Settings: A Survey of Autism Professionals
Individuals with autism spectrum disorder (ASD) display deficits in communication and social interaction that can impact their ability to function in daily environments. To remediate these deficits, it is critical for professionals to use effective interventions. While there are many evidence-based practices (EBPs) identified for ASD (e.g., video modeling), the adoption of these EBPs may not occur automatically. Existing research suggests professionals have a generally favorable impression of video modeling. However, little research has examined opinions and applied use of video modeling, which was the purpose of the present study. Using survey methodology, data were collected from 510 professionals in various disciplines (e.g., special educators, speech-language pathologists [SLPs], and behavior analysts [BCBAs]). Data were analyzed primarily via factor analysis and multiple regression. Factor analysis was used to examine the underlying structure of the instrument, revealing two predominant factors: (1) interest in and (2) perceived accessibility of video modeling. Multiple regression was used to examine which demographic characteristics (e.g., age and years of experience) were associated with each factor. Results indicated that BCBAs and SLPs perceived video modeling as more accessible. In terms of interest, professionals who worked with preschool-aged students, who worked in a suburban location, and who had an extended family member with ASD showed higher interest in video modeling. Implications for practice and future research are discussed.
An Argument in Favor of the Saxhorn Basse (French Tuba) in the Modern Symphony Orchestra
The French tuba was a much-needed addition to the brasswind musical instrument family, adding depth, projection and a unique color to French orchestral literature. Its ancestors the serpent and ophicleide both lacked the tonal stability and sonic power to adequately present the bass wind role in a robust orchestra. Through the efforts of its developer and patent-holder Adolphe Sax, the French tuba made converts among players and composers, effectively creating its own niche in music history. Musical tastes change however, and the French tuba has been largely supplanted by tubists using instruments twice its size. Since French composers composed specifically with the distinct timbre of the French tuba in mind, this unique and characteristic musical entity deserves a resurgence in performances of French orchestral repertoire.
Artistic Decision Making and Implications for Engaging Theatrically Gifted and Talented Students in Non-Arts Classes
This cognitive ethnographic study explored the mental processes that professional actors used when making artistic choices while engaged in creative practices to begin a conversation about how the theatrically gifted and talented population is viewed, researched, and educated in non-arts subjects. Professional actors at two sites were observed, videotaped, and interviewed over several rehearsals during play production. The major thematic findings indicated that artistic decision making results from actors engaging in a cyclical process of private work, affective validation, and collaboration. Implications for teaching theatrically gifted students call for classroom environments and processes that echo theatrical rehearsal structures, while engaging the imagination through personal connection and discovery.
Assessing Rainfall Interception by Urban Tree Canopies in Denton, Texas
Rainfall interception is one mechanism by which tree canopies can reduce surface runoff in urban areas. The objectives of this research were to: 1) quantify rainfall interception by urban tree canopies, and 2) determine the influence of vegetation and microenvironmental factors on rainfall interception rates. In the city of Denton, Texas, 30 mature post oak (Quercus stellata) and blackjack oak (Quercus marilandica) trees were selected for study. Trees were assigned to one of three categories: clusters of trees on greenspace (CG), isolated trees on greenspace (IG), and isolated trees surrounded by pavement (IP). Throughfall (the volume of water that travels through the canopy and reaches the soil surface) collectors were placed beneath these trees and rainfall collectors were placed in nearby open areas. Throughfall and rainfall were collected daily from 19 March to 4 July. Interception was calculated as the difference between throughfall and gross rainfall. Over the study period, there were 27 days with measurable rainfall; daily rainfall ranged from 1-51 mm. Over the sampling period, rainfall interception for individual trees ranged from -10% to 49%, indicating high spatial variability in interception. Percent interception was highest for the CG treatment (22.7 ± 3.8 SE), intermediate for IG (27.4 ± 2.3 SE), and lowest for IP (9.1 ± 4.9 SE). Factors like wind exposure, wind-driven rain and overall tree health may help explain this variability. This research will contribute to our knowledge of hydrological fluxes in urban areas and the role of urban green infrastructure in stormwater runoff mitigation.
Automated GUI Tests Generation for Android Apps Using Q-learning
Mobile applications are growing in popularity and pose new problems in the area of software testing. In particular, mobile applications heavily depend upon user interactions and a dynamically changing environment of system events. In this thesis, we focus on user-driven events and use Q-learning, a reinforcement machine learning algorithm, to generate tests for Android applications under test (AUT). We implement a framework that automates the generation of GUI test cases by using our Q-learning approach and compare it to a uniform random (UR) implementation. A novel feature of our approach is that we generate user-driven event sequences through the GUI, without the source code or the model of the AUT. Hence, considerable amount of cost and time are saved by avoiding the need for model generation for generating the tests. Our results show that the systematic path exploration used by Q-learning results in higher average code coverage in comparison to the uniform random approach.
Becoming Successful in Education: Beating the Odds, Despite a Background Entrenched in Poverty
The purpose of this study was to investigate the impact of three relationships on academic achievement in mathematics in students of poverty. The three factors that were examined included: teacher-student relationships, parent-student relationships and peer- student relationships. The driving question for the research was as follows: Do external factors such as teacher-student relationships, parent-student relationships and peer-student relationships lead to academic success for students of poverty? The study employed a non-experimental, quantitative approach and utilized longitudinal data from a national database High School Longitudinal Study (HSLS) used a sample of 944 public and private high schools across the USA. A total 0f 28,240 were represented in the survey. Of these 28,240 students, 2641 were used in this study as identified by parental income below the poverty threshold. The outcome of the study indicated that there was little or no correlation between the three relationships and mathematics achievement (academic success). Correlations between the dependent variable (math achievement) and the independent variables even though some were statistically significant their weights had no concrete significance. The study recommends that several initiatives can be instated in schools to support and enhance academic achievement in students of poverty.
Bodies of Evidence: A Qualitative Analysis of the Lived Experiences of Female Central American and Mexican Asylum Seekers in Dallas
This work addresses the experiences of female asylum seekers from Central and Mexico currently living in Dallas, TX. The main purpose is to analyze how these women engage in the gendered processes of both migrating to and accessing legal resources and protection within the United States. As the women move through male-dominated spaces in their home country, the borderlands, and the asylum court they must challenge the patriarchal institutions that attempt to silence their narratives and criminalize their bodies. Their physical wounds become evidence in the courtroom, while outside of the courtroom their movements are monitored and tracked through multiple mechanisms of state control: ankle monitors, detention centers, ICE check-ins. They face intersectional discrimination as they are targeted as both women and immigrants. However, these female asylum seekers are not victims. They constantly display agency as they represent themselves in court, find solace in their faith, and form community with each other.
Brain Computer Interface (BCI) Applications: Privacy Threats and Countermeasures
In recent years, brain computer interfaces (BCIs) have gained popularity in non-medical domains such as the gaming, entertainment, personal health, and marketing industries. A growing number of companies offer various inexpensive consumer grade BCIs and some of these companies have recently introduced the concept of BCI "App stores" in order to facilitate the expansion of BCI applications and provide software development kits (SDKs) for other developers to create new applications for their devices. The BCI applications access to users' unique brainwave signals, which consequently allows them to make inferences about users' thoughts and mental processes. Since there are no specific standards that govern the development of BCI applications, its users are at the risk of privacy breaches. In this work, we perform first comprehensive analysis of BCI App stores including software development kits (SDKs), application programming interfaces (APIs), and BCI applications w.r.t privacy issues. The goal is to understand the way brainwave signals are handled by BCI applications and what threats to the privacy of users exist. Our findings show that most applications have unrestricted access to users' brainwave signals and can easily extract private information about their users without them even noticing. We discuss potential privacy threats posed by current practices used in BCI App stores and then describe some countermeasures that could be used to mitigate the privacy threats. Also, develop a prototype which gives the BCI app users a choice to restrict their brain signal dynamically.
Building an Effective Piano Technique while Avoiding Injury: A Comparison of the Exercises in Alfred Cortot's "Rational Principles of Pianoforte Technique" and Carl Tausig's "Daily Studies for the Pianoforte"
It is the teacher's responsibility to guide students in building an effective and injury-free piano technique. Improper technique, poor training and bad posture at the instrument all may cause problems such as lack of muscle control, weakness, or tension in the hands. Many teachers are interested in finding information about specific exercises dealing with finger strengthening, stretching, and warm-up strategies, as well as guidelines for safe practicing. It is therefore important for both teachers and students to understand how to build a technique from the earliest years of instruction. Carl Tausig (1841-1871) and Alfred Cortot (1877-1962) both contributed to the development of piano technique by writing books that include a significant number of exercises and excerpts. Their books incorporate detailed instructions on how to play each exercise effectively and without fatigue. Subsequently, Heinrich Ehrlich (1822-1899) collected and systematically arranged Tausig's notes, complementing them with detailed information on how to play Tausig's exercises without causing injury. This dissertation compares and contrasts the exercises found in Alfred Cortot's book, Rational Principles of Pianoforte Technique, and Carl Tausig's book, Daily Studies for the Pianoforte. The latter is based on the practical guidebook, How to Practise on the Piano: Reflections and Suggestions, written by Heinrich Ehrlich. Included in this study are references to the performing arts medical literature dealing with pianists' injuries. By comparing two different historical piano methods and considering their effectiveness in light of modern medical performance research, this dissertation aims to help teachers to determine which methods might be better for students to build a solid piano technique without injuring themselves.
Cardiovascular Response to a Behavioral Restraint Challenge: Urge Magnitude Influence in Men and Women
Agtarap, Wright, Mlynski, Hammad, and Blackledge took an initial step in providing support for the predictive validity of a new conceptual analysis concerned with behavioral restraint, defined as active resistance against a behavioral impulse or urge. The current study was designed to partially replicate and extend findings from their study, employing a common film protocol and a procedure for inducing low- and high levels of fatigue. Analyses on key data indicated that the fatigue manipulation was ineffective. On the other hand, they supported the suggestion that behavioral restraint should be proportional to the strength of an urge being resisted so long as success is perceived as possible and worthwhile. Analyses also provided evidence of gender differences for this behavioral restraint task. Women showed relatively enhanced CV responses to my manipulation of urge magnitude, performed less well, rated the behavioral restraint challenge as harder, and rated success on the more difficult behavioral restraint task as more important. A broad indication is that men and women can differ in the strength of impulses they experience in response to stimulus presentations as well as in the importance they place on resisting the impulses.
Case Studies to Learn Human Mapping Strategies in a Variety of Coarse-Grained Reconfigurable Architectures
Computer hardware and algorithm design have seen significant progress over the years. It is also seen that there are several domains in which humans are more efficient than computers. For example in image recognition, image tagging, natural language understanding and processing, humans often find complicated algorithms quite easy to grasp. This thesis presents the different case studies to learn human mapping strategy to solve the mapping problem in the area of coarse-grained reconfigurable architectures (CGRAs). To achieve optimum level performance and consume less energy in CGRAs, place and route problem has always been a major concern. Making use of human characteristics can be helpful in problems as such, through pattern recognition and experience. Therefore to conduct the case studies a computer mapping game called UNTANGLED was analyzed as a medium to convey insights of human mapping strategies in a variety of architectures. The purpose of this research was to learn from humans so that we can come up with better algorithms to outperform the existing algorithms. We observed how human strategies vary as we present them with different architectures, different architectures with constraints, different visualization as well as how the quality of solution changes with experience. In this work all the case studies obtained from exploiting human strategies provide useful feedback that can improve upon existing algorithms. These insights can be adapted to find the best architectural solution for a particular domain and for future research directions for mapping onto mesh-and- stripe based CGRAs.
Catalytic Properties and Mechanical Behavior of Metallic Glass Powders
Lack of crystalline order and microstructural features such as grain/grain-boundary in metallic glasses results in a suite of remarkable attributes including very high strength, close to theoretical elasticity, high corrosion and wear resistance, and soft magnetic properties. By altering the morphology and tuning of composition, MGs may be transformed into high-performance catalytic materials. In this study, the catalytic properties of metallic glass powders were demonstrated in dissociating toxic organic chemicals such as AZO dye. BMG powders showed superior performance compared to state of the art crystalline iron because of their high catalytic activity, durability, and reusability. To enhance the catalytic properties, high energy mechanical milling was performed to increase the surface area and defect density. Iron-based bulk metallic glass (BMG) of composition Fe48Cr15Mo14Y2C15B6 was used because of its low cost and ability to make large surface area by high energy ball milling. AZO dye was degraded in less than 20 minutes for the 9 hours milled Fe-BMG. However, subsequent increase in ball milling time resulted in devitrification and loss of catalytic activity as measured using UV-Visible spectroscopy. Aluminum-based bulk metallic glass (Al-BMG) powder of composition Al82Fe3Ni8Y7 was synthesized by arc-melting the constituent elements followed by gas-atomization. The particle size and morphology were similar to Fe-BMG with a fully amorphous structure. A small percentage of transition metal constituents (Fe and Ni) in a mostly aluminum alloy showed high catalytic activity, with no toxic by-products and no change in surface characteristics. Al-alloy particles, being light-weight, were easily dispersed in aqueous medium and accelerated the redox reactions. The mechanism of dye dissociation was studied using Raman and Infrared (IR) spectroscopy. Breaking of -C-H- and - C-N- bonds of AZO dye was found to be the primary mechanism. Mechanical behavior of individual BMG particles was evaluated by in situ pico-indentation in a scanning electron ...
Catastrophe in Permanence: Benjamin's Natural History of Environmental Crisis
Walter Benjamin warned in 1940 of a certain inconspicuous threat to political thinking, not least of all to materialism, that takes progress as an historical norm. Implicit in this conception is what he describes as an empty continuum of time along which the prevailing tradition chronicles its own mythic development and drains everyday life of genuine historical experience. The myth of progressive history advances insidiously today in consumeristic and technocratic attempts at reconciling cultural imagery with organic nature. In this dissertation, I pursue the contradictions of such images as they crystallize around the natural history of twenty-first century commodity society, where promises of ecological remediation, sustainable urban development, and climate change mitigation have yet to introduce a true crisis of historical experience to the ongoing environmental crisis of capitalism. A more radical way of seeing the cultural representation of nature would, I argue, penetrate its mythic determination by market forces and bear witness to the natural-historical ruins and traces that constitute, in Benjamin's terms, a single "catastrophe" where others perceive historical continuity. I argue that Benjamin's critique of progress is instructive to interpreting those utopian dreams, ablaze in consumer life and technological fantasy, that recent decades of growing environmental concern have channeled into the recovery of an experience of the natural world. His dialectics of nature and alienated history confront the wish-image of organic abundance with the transience of its appropriated expression in the commodity-form. Drawing together this confrontation with a varied literature on collective memory, nature, and the city, I suggest that our poverty of experience is more than simply a technical, economic, or even ecological problem, but rather follows from the commodification of history itself. The goal of this work is to reflect upon the potentiality of communal politics that subsist not in rushing headlong into a progressive ...
Characteristics of Mothers among Counselor Education Faculty
Pre-tenured faculty in higher education and as well as mothers have reportedly struggled with low wellness levels, high demands, little social support, and an imbalance of work and home life. Mothers in higher education and in counselor education have reported struggling with work-life balance, high scholarly productivity, and long hours as well as the emotional and physical energy demands of working with counselors-in-training. A search of the professional literature revealed a paucity of quantitative research regarding demographic characteristics, wellness levels, and social support levels of mothers among counselor education faculty (MCEs). Participants for this study were faculties of counselor education programs recruited from the Holland List of Counseling Programs and from the Council for Accreditation of Counseling and Related Educational Programs online directory. A total of 180 MCEs participated (aged 29-63, with mean age 40.6 years; 83% Caucasian, 8% other, 5% African American, 3% Hispanic, <1% Asian). Results showed that faculty rank did not account for a significant difference among wellness scores of MCEs and that reported social support, tenure or non-tenure track, number of children in the care of MCEs, number of children under age 8, number of publications, and teaching workload accounted for 14% of the variance in wellness levels of MCEs. Specifically, reported higher teaching workload (β = -.194, rs2 = .35, p = .012) and higher social support (β = -.258, rs2 = .36, p = <.001) were found to be significant predictors of lower wellness levels among MCEs, both with small effects. Based on these results, MCEs may benefit from advocating that their departments and universities adapt to their unique needs to improve their levels of wellness and social support through mentoring, which, in turn, may result in not only their own increased productivity but also their students' increased wellness levels.
The Civic Roles of Neighborhood Associations in Seoul, Korea: Implications for Urban Governance
This dissertation answers three research questions: "What differences and similarities exist among neighborhood associations in the United States, Japan, and Seoul, South Korea?," "What are the civic roles of neighborhood associations in apartment complexes in Seoul, South Korea?," and "What factors promote neighborhood associations to play civic roles in urban governance?" To answer the first question, this research analyzes the purposes, governance structures, roles, and legal substance of neighborhood associations by reviewing previous studies, public and legal documents, court cases in the U.S., and by conducting interviews. To answer the second and the third questions, a 2016 survey, "Understanding the Roles of Neighborhood Associations in Urban Governance" was conducted with 154 representatives of neighborhood associations in Seoul. Social capital theory, government failure theory, and third party government theory were used to create hypotheses that test proposed relationships about neighborhood interactions, community characteristics, and the civic roles played by neighborhood associations. The findings show that neighborhood associations have adopted several roles including service provider, partner in public service delivery for local government, and advocate for residents in urban governance. The findings also show that social capital created by neighborhood interactions and community characteristics facilitates neighborhood associations to play civic roles in urban governance.
A Cognitive Radio Application through Opportunistic Spectrum Access
In wireless communication systems, one of the most important resources being focused on all the researchers is spectrum. A cognitive radio (CR) system is one of the efficient ways to access the radio spectrum opportunistically, and efficiently use the available underutilized licensed spectrum. Spectrum utilization can be significantly enhanced by developing more applications with adopting CR technology. CR systems are implemented using a radio technology called software-defined radios (SDR). SDR provides a flexible and cost-effective solution to fulfil the requirements of end users. We can see a lot of innovations in Internet of Things (IoT) and increasing number of smart devices. Hence, a CR system application involving an IoT device is studied in this thesis. Opportunistic spectrum access involves two tasks of CR system: spectrum sensing and dynamic spectrum access. The functioning of the CR system is rest upon the spectrum sensing. There are different spectrum sensing techniques used to detect the spectrum holes and a few of them are discussed here in this thesis. The simplest and easiest to implement energy detection spectrum sensing technique is used here to implement the CR system. Dynamic spectrum access involves different models and strategies to access the spectrum. Amongst the available models, an interweave model is more challenging and is used in this thesis. Interweave model needs effective spectrum sensing before accessing the spectrum opportunistically. The system designed and simulated in this thesis is capable of transmitting an output from an IoT device using USRP and GNU radio through accessing the radio spectrum opportunistically.
Collaboration in scholarly communication: Opportunities to normalize open access
This article discusses scholarly communication from a holistic perspective and includes some strategies implemented at University of North Texas Libraries.
Collective Security and Coalition: British Grand Strategy, 1783-1797
On 1 February 1793, the National Convention of Revolutionary France declared war on Great Britain and the Netherlands, expanding the list of France's enemies in the War of the First Coalition. Although British Prime Minister William Pitt the Younger had predicted fifteen years of peace one year earlier, the French declaration of war initiated nearly a quarter century of war between Britain and France with only a brief respite during the Peace of Amiens. Britain entered the war amid both a nadir in British diplomacy and internal political divisions over the direction of British foreign policy. After becoming prime minister in 1783 in the aftermath of the War of American Independence, Pitt pursued financial and naval reform to recover British strength and cautious interventionism to end Britain's diplomatic isolation in Europe. He hoped to create a collective security system based on the principles of the territorial status quo, trade agreements, neutral rights, and resolution of diplomatic disputes through mediation - armed mediation if necessary. While his domestic measures largely met with success, Pitt's foreign policy suffered from a paucity of like-minded allies, contradictions between traditional hostility to France and emergent opposition to Russian expansion, Britain's limited ability to project power on the continent, and the even more limited will of Parliament to support such interventionism. Nevertheless, Pitt's collective security goal continued to shape British strategy in the War of the First Coalition, and the same challenges continued to plague the British war effort. This led to failure in the war and left the British fighting on alone after the Treaty of Campo Formio secured peace between France and its last continental foe, Austria, on 18 October 1797.
Coming Out: The Lived Experiences of LGB College Students who Feel Supported by Their Parents
The purpose of this phenomenological study was to explore how LGB college students created meaning out of their coming out process to their parents. I recruited LGB college students who perceived support from their parents during their coming out process and asked the following research question: What are the lived experiences of LGB college students who have experienced support from their parents during the coming out process? Seven White (n = 4), African American (n = 2), and Hispanic (n = 1) college students, three men and four women aged 18-24 years, shared narratives that included time periods before, during, and after their coming out disclosures to their parents. Using an adapted phenomenological analysis, I identified nine major themes: awareness of feeling different, positive relationship with parents prior to coming out, college impacting the coming out process, feeling unsure of how parents would respond to disclosure, parents assuring continued loved and acceptance, parents affirming LGB identity, increased relational depth with parents, increased sense of authenticity, and an appreciation for family's response and support. The findings provide insight into how counselors might work most beneficially with LGB college students and their parents around the coming out process. Opportunities for future research and limitations of the study are discussed.
A Community Based Assessment: An Analysis of Community Based Tourism in Kalache and Hulgol, India
This study incorporated a community based assessment with a focus on community based tourism in Kalache and Hulgol, India. Kalache and Hulgol are two agrarian based communities located in the environmentally significant region of the Western Ghats. Each of these communities has considered community based tourism as a means to reduce urban youth outmigration, to diversify economic resources, and to encourage the empowerment of women. The primary goals of this study were to understand the community issues and objectives, to determine the level of support for tourism development, to determine participant attitudes toward tourism, and to determine the obstacles to tourism development. The findings of this project address the complexity of operating in the tourism industry, the impacts of tourism, and the use of community based tourism models in support of sustainable tourism.
Community College Student Retention and Completion based on Financial Expenditures and Hispanic-Serving Status
Despite declining community college funding being allocated increasingly on the basis of student success, U.S. community college student retention and completion rates over the past decade have either remained steady or decreased, especially for Latino students. Using descriptive statistics and multiple regression models with secondary data procured from the Integrated Postsecondary Education Data System (IPEDS), I analyzed student success rates—full time student retention and completion rates—based on community college financial allocations and Hispanic-serving institution (HSI) status. To equitably analyze community colleges in the sample (n = 909), I separated them into four groups based on institutional size as defined by the Carnegie Classification. Descriptive results indicated that instructional divisions spent an average of 43% of the college's total allocated budget—often more than three times the allocated budget of any other division. Regression results indicated that instructional expenditures had the most consistent impact on student success regardless of college size and that scholarship expenditures and academic support expenditures generally had a negative impact on student retention and completion rates. Regarding Latino student success in particular, findings indicated that the manner in which colleges allocated their funds impacted only small and medium-sized community colleges. Of the nine different types of institutional expenditures, only student services expenditures and public services expenditures had a statistically significant impact on Latino student success. Additionally regression analysis indicated that community college HSI status did not have a large impact on overall full-time student retention and completion rates but did have a significant impact on full-time Hispanic student retention and completion rates for all institution sizes. Findings of this study confirmed that HSI status does impact Latino student success in public community colleges. This finding is consistent with prior studies on the positive impact of instructional expenditures on student success rates. Further research on the specific elements within ...
A Comparative Evaluation of Matrix Training Arrangements
A common goal of instructional techniques is to teach skills effectively and efficiently. Matrix training techniques are both effective and efficient as they allow for the emergence of untrained responding to novel stimulus arrangements, a phenomenon known as recombinative generalization. However, it is unclear which type of matrix arrangement best promotes recombinative generalization. The current study compared two common matrix training approaches, an overlapping (OV) design and a non-overlapping (NOV) design, with respect to arranging relations targeted for training. We conducted a replication evaluation of a Wilshire and Toussaint study, and taught two typically-developing preschoolers compound object-action labels in Spanish and used either an OV or NOV matrix training design. Results from both studies demonstrated the participant trained with an OV design produced recombinative generalization and participants trained with a NOV design produced significantly low levels of emergence or none at all. These results suggest that an OV matrix design facilitates recombinative generalization more effectively than a NOV design. Implications for instructional arrangements are discussed.
Comparing Media Usage of Binary and Non-Binary Transgender Individuals when Discovering and Describing Gender Identity
This study was conducted through in-depth interviews to examine potential differences between binary-aligned transgender individuals and non-binary individuals in regards to media usage when learning about, articulating, and explaining their gender identity. Results showed numerous differences between transgender people with binary-aligned and non-binary gender identifications in regards to social media preferences and differences in perceived media importance and effects. Additional information was found in regards to the age at which gender identity is articulated and the importance of individuality in comparison to one's gender identity.
Composer/Performer Collaboration as Seen in the Solo Piano Part of Percy Grainger's Edition of the Edvard Grieg Piano Concerto in A Minor, Opus 16
The purpose of this document is threefold. First, it demonstrates what Grieg contributes to the musical text compared with the original Peters edition, particularly, those additions that refer to expression, interpretation, and style. Second, this document focuses on presenting Grainger's changes that were approved by Grieg. Third, the document evaluates Grainger's own suggestions for pedaling, hand redistribution and fingering, addition of notes, tempo markings, and other performance guidelines.
Consequences of Coworker Bullying: A Bystander Perspective
Previous research on workplace bullying primarily focuses on two main actors – the bully and the victim – while neglecting a third actor: the bystander of the bullying. The prevalence of workplace bullying is increasing across organizations, resulting in more employees becoming subjected to the effects of workplace bullying. Furthermore, witnessing coworker-on-coworker bullying is likely to influence the relationships that the bystander has with the two coworkers involved in the bullying episode. Two areas are proposed to investigate their effect on the coworker bystander: coworker interpersonal justice and personal identification with coworkers. Coworker interpersonal justice involves the perceived fairness between coworkers, while personal identification refers to how these bystanders identify with the specific actors of the bullying event. In addition to work-related outcomes, bystanders are affected at a personal level. That is, being exposed to bullying situations causes these bystanders to alter their anxiety levels and their core affect, with core affect being a precursor to moods and emotions. In addition to the aforementioned outcomes of witnessing a coworker bullying incident, there are also contextual aspects which may influence these relationships. Personal-level factors, such as a bystander's empathy and sense of coherence (i.e., coping mechanisms), may influence the effect of witnessing a coworker being bullied. Similarly, the gender of the victim in relation to the gender of the bystander may also play a role. Using affective events theory, I investigate how witnessing coworker bullying in the workplace effects bystanders. This research employs a 2 x 2 experimental design with multi-wave data collection and an in-person lab session to test the proposed hypotheses. AET is operationalized by creating a fictional coworker bullying situation in which observers are either exposed to the bullying situation or not. This research offers several contributions to the management literature as well as to practitioners. First, it ...
Construct Validation and Measurement Invariance of the Athletic Coping Skills Inventory for Educational Settings
The present study examined the factor structure and measurement invariance of the revised version of the Athletic Coping Skills Inventory (ACSI-28), following adjustment of the wording of items such that they were appropriate to assess Coping Skills in an educational setting. A sample of middle school students (n = 1,037) completed the revised inventory. An initial confirmatory factor analysis led to the hypothesis of a better fitting model with two items removed. Reliability of the subscales and the instrument as a whole was acceptable. Items were examined for sex invariance with differential item functioning (DIF) using item response theory, and five items were flagged for significant sex non-invariance. Following removal of these items, comparison of the mean differences between male and female coping scores revealed that there was no significant difference between the two groups. Further examination of the generalizability of the coping construct and the potential transfer of psychosocial skills between athletic and academic settings are warranted.
A Constructional Approach to Establishing and Maintaining Calm Canine Behavior
Very few behavior-change programs with canines produce effects that persist beyond the training condition. The present study is an experimental demonstration of a constructional program that established calm patterns of behavior as alternatives to hyperactive ones. Three dogs that exhibited hyperactive patterns were chosen as subjects. Seven conditions common to canine-caretaker relationships were used to determine which factors resulted in the hyperactive patterns. Then, sitting and lying down were taught as beginning points using touch as a reinforcer. The final behavior, maintained by naturally occurring reinforcers, was established errorlessly. The study used a control-analysis strategy of behavior change with a changing-criterion design. The intervention resulted in an immediate reduction in hyperactivity and an increase in sitting and lying down for all dogs.
A Content Analysis of Medical School Problem-Based Learning Cases
Problem-based learning (PBL) was developed for use in medical education to incorporate more active, learner-centered instruction. Central to problem-based learning is the problem, which in medical education is usually case a case presentation, revealed in stages to allow learners to form and research learning objectives. The purpose of this study was to identify themes present across the PBL cases, including the patient-centeredness of the cases. Content analysis was used to examine 62 PBL cases that comprised the first and second years' core curriculum at a public medical school. The cases included a patient population similar to the local population, but care was more hospital-centric than would be expected from the actual patterns of medical utilization in the United States. Analyzing along two axes of patient-centeredness, the PBL cases demonstrated a good understanding of the patient (knowing the patient), but other qualities such as shared decision making was not as exemplified. Medical educators can use the results to understand elements that contribute to patient-centeredness and apply the analysis framework to evaluate future cases.
Content and Temporal Analysis of Communications to Predict Task Cohesion in Software Development Global Teams
Virtual teams in industry are increasingly being used to develop software, create products, and accomplish tasks. However, analyzing those collaborations under same-time/different-place conditions is well-known to be difficult. In order to overcome some of these challenges, this research was concerned with the study of collaboration-based, content-based and temporal measures and their ability to predict cohesion within global software development projects. Messages were collected from three software development projects that involved students from two different countries. The similarities and quantities of these interactions were computed and analyzed at individual and group levels. Results of interaction-based metrics showed that the collaboration variables most related to Task Cohesion were Linguistic Style Matching and Information Exchange. The study also found that Information Exchange rate and Reply rate have a significant and positive correlation to Task Cohesion, a factor used to describe participants' engagement in the global software development process. This relation was also found at the Group level. All these results suggest that metrics based on rate can be very useful for predicting cohesion in virtual groups. Similarly, content features based on communication categories were used to improve the identification of Task Cohesion levels. This model showed mixed results, since only Work similarity and Social rate were found to be correlated with Task Cohesion. This result can be explained by how a group's cohesiveness is often associated with fairness and trust, and that these two factors are often achieved by increased social and work communications. Also, at a group-level, all models were found correlated to Task Cohesion, specifically, Similarity+Rate, which suggests that models that include social and work communication categories are also good predictors of team cohesiveness. Finally, temporal interaction similarity measures were calculated to assess their prediction capabilities in a global setting. Results showed a significant negative correlation between the Pacing Rate and ...
A Content Originality Analysis of HRD Focused Dissertations and Published Academic Articles using TurnItIn Plagiarism Detection Software
This empirical exploratory study quantitatively analyzed content similarity indices (potential plagiarism) from a corpus consisting of 360 dissertations and 360 published articles. The population was defined using the filtering search criteria human resource development, training and development, organizational development, career development, or HRD. This study described in detail the process of collecting content similarity analysis (CSA) metadata using Turnitin software (www.turnitin.com). This researcher conducted robust descriptive statistics, a Wilcoxon signed-rank statistic between the similarity indices before and after false positives were excluded, and a multinomial logistic regression analysis to predict levels of plagiarism for the dissertations and the published articles. The corpus of dissertations had an adjusted rate of document similarity (potential plagiarism) of M = 9%, (SD = 6%) with 88.1% of the dissertations in the low level of plagiarism, 9.7% in the high and 2.2% in the excessive group. The corpus of published articles had an adjusted rate of document similarity (potential plagiarism) of M = 11%, (SD = 10%) with 79.2% of the published articles in the low level of plagiarism, 12.8% in the high and 8.1% in the excessive group. Most of the difference between the dissertations and published articles were attributed to plagiarism-of-self issues which were absent in the dissertations. Statistics were also conducted which returned a statistically significant justification for employing the investigative process of removing false positives, thereby adjusting the Turnitin results. This study also found two independent variables (reference and word counts) that predicted dissertation membership in the high (.15-.24) and excessive level (.25-1.00) of plagiarism and published article membership in the excessive level (.25-1.00) of plagiarism. I used multinomial logistic regression to establish the optimal prediction model. The multinomial logistic regression results for the dissertations returned a Nagelkerke pseudo R2 of .169 and for the published articles a Nagelkerke pseudo R2 ...
Creating Discussion: An Auteur Analysis of Films Directed by Adrian Lyne
This thesis examines the various "signature" threads that are present within the "oeuvre" of the Hollywood filmmaker Adrian Lyne. The goal of this thesis is to showcase both how and why Lyne can be thought of as an auteur and to open up his films to new and previously unexplored meanings. Lyne's eight feature films are analyzed in-depth individually and in comparison to one another from a variety of theoretical frameworks and points of focus in each of the body chapters.
Culturally Competent Evaluations
Significant growth in the number of English language learners (ELLs) in U.S. schools is anticipated to continue, demanding that educators and evaluators have the skills necessary to distinguishing language difference from disability and provide appropriate services to these students. However, little research exists examining the role of evaluator's cultural competence in evaluating ELLs for special education; furthermore, what does exist shows that many evaluators report low levels of self-efficacy as it relates to assessing ELLs. The first chapter of the dissertation reports on a review of 21 articles conducted to address best practices for evaluating culturally and linguistically diverse (CLD) learners, evaluator self-efficacy, and recommendations for conducting culturally competent assessments and evaluations. The majority of the articles identified in this review focused on recommendations for best practices for conducting evaluations rather than reporting empirical findings related to the topic. Only one study was identified that focused on appropriate training needed by evaluation staff to effectively discriminate between language difference and a disability. Based on the findings of this review, additional research, using a rigorous methodology is needed. Addressing that need, the second chapter reports the results of a study conducted to examine the effectiveness of Project PEAC3E (Preparing Evaluators to Accurately Conduct Culturally Competent Evaluations), a reform-oriented professional development model, using case-based activities designed to increase evaluators' sense of self-efficacy, cultural competence, and the accuracy of evaluator eligibility decisions for English language learners (ELLs). The study found that Project PEAC3E was effective in increasing evaluator self-efficacy.
Design Considerations and Implementation of Portable Mass Spectrometers for Environmental Applications
Portable mass spectrometers provide a unique opportunity to obtain in situ measurements. This minimizes need for sample collection or in laboratory analysis. Membrane Inlet Mass Spectrometry (MIMS) utilizing a semi permeable membrane for selective rapid introduction for analysis. Polydimethylsiloxane membranes have been proven to be robust in selecting for aromatic chemistries. Advances in front end design have allowed for increased sensitivity, rapid sample analysis, and on line measurements. Applications of the membrane inlet technique have been applied to environmental detection of clandestine drug chemistries and pollutants. Emplacement of a mass spectrometer unit in a vehicle has allowed for large areas to be mapped, obtaining a rapid snapshot of the various concentrations and types of environmental pollutants present. Further refinements and miniaturization have allowed for a backpackable system for analysis in remote harsh environments. Inclusion of atmospheric dispersion modeling has yielded an analytical method of approximating upwind source locations, which has law enforcement, military, and environmental applications. The atmospheric dispersion theories have further been applied to an earth based separation, whereby chemical properties are used to approximate atmospheric mobility, and chemistries are further identified has a portable mass spectrometer is traversed closer to a point source.
Design, Modeling, and Experiment of a Piezoelectric Pressure Sensor based on a Thickness-Shear Mode Crystal Resonator
This thesis presents the design, modeling, and experiment of a novel pressure sensor using a dual-mode AT-cut quartz crystal resonator with beat frequency analysis based temperature compensation technique. The proposed sensor can measure pressure and temperature simultaneously by a single AT-cut quartz resonator. Apart from AT-cut quartz crystal, a newly developed Langasite (LGS) crystal resonator is also considered in the proposed pressure sensor design, since LGS can operate in a higher temperature environment than AT-cut quartz crystal. The pressure sensor is designed using CAD (computer aided design) software and CAE software - COMSOL Multiphysics. Finite element analysis (FEA) of the pressure sensor is performed to analyze the stress- strain of the sensor's mechanical structure. A 3D printing prototype of the sensor is fabricated and the proposed sensing principle is verified using a force-frequency analysis apparatus. Next to the 3D printing model verification, the pressure sensor with stainless steel housing has been fabricated with inbuilt crystal oscillator circuit. The oscillator circuit is used to excite the piezo crystal resonator at its fundamental vibrational mode and give the frequency as an output signal. Based on the FEA and experimental results, it has been concluded that the maximum pressure that the sensor can measure is 45 (psi). The pressure test results performed on the stainless steel product shows a highly linear relationship between the input (pressure) and the output (frequency).
Design of a Lower Extremity Exoskeleton to Increase Knee ROM during Valgus Bracing for Osteoarthritic Gait
Knee osteoarthritis (KOA) is the primary cause of chronic immobility in populations over the age of 65. It is a joint degenerative disease in which the articular cartilage in the knee joint wears down over time, leading to symptoms of pain, instability, joint stiffness, and misalignment of the lower extremities. Without intervention, these symptoms gradually worsen over time, decreasing the overall knee range of motion (ROM) and ability to walk. Current clinical interventions include offloading braces, which mechanically realign the lower extremities to alleviate the pain experienced in the medial compartment of the knee joint. Though these braces have proven effective in pain management, studies have shown a significant decrease in knee ROM while using the brace. Concurrently, development of active exoskeletons for rehabilitative gait has increased within recent years in efforts to provide patients with a more effective intervention for dealing with KOA. Though some developed exoskeletons are promising in their efficacy of fostering gait therapy, these devices are heavy, tethered, difficult to control, unavailable to patients, or costly due to the number of complicated components used to manufacture the device. However, the idea that an active component can improve gait therapy for patients motivates this study. This study proposes the design of an adjustable lower extremity exoskeleton which features a single linear actuator adapted onto a commercially available offloading brace. This design hopes to provide patients with pain alleviation from the brace, while also actively driving the knee through flexion and extension. The design and execution of this exoskeleton was accomplished by 3D computer simulation, 3D CAD modeling, and rapid prototyping techniques. The exoskeleton features 3D printed, ABS plastic struts and supports to achieve successful adaptation of the linear actuator to the brace and an electromechanical system with a rechargeable operating capacity of 7 hours. Design validation was ...
Design of a Machine Condition Monitoring System with Bluetooth Low Energy
This thesis discusses the design considerations for a machine conditioning sensor utilizing Bluetooth low energy (BLE).
Design Principle on Carbon Nanomaterials Electrocatalysts for Energy Storage and Conversion
We are facing an energy crisis because of the limitation of the fossil fuel and the pollution caused by burning it. Clean energy technologies, such as fuel cells and metal-air batteries, are studied extensively because of this high efficiency and less pollution. Oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) are essential in the process of energy storage and conversion, and noble metals (e.g. Pt) are needed to catalyze the critical chemical reactions in these devices. Functionalized carbon nanomaterials such as heteroatom-doped and molecule-adsorbed graphene can be used as metal-free catalysts to replace the expensive and scarce platinum-based catalysts for the energy storage and conversion. Traditionally, experimental studies on the catalytic performance of carbon nanomaterials have been conducted extensively, however, there is a lack of computational studies to guide the experiments for rapid search for the best catalysts. In addition, theoretical mechanism and the rational design principle towards ORR and OER also need to be fully understood. In this dissertation, density functional theory calculations are performed to calculate the thermodynamic and electrochemical properties of heteroatom-doped graphene and molecule-adsorbed graphene for ORR and OER. Gibb's free energy, overpotential, charge transfer and edge effect are evaluated. The charge transfer analysis show the positive charges on the graphene surface caused by the heteroatom, hetero-edges and the adsorbed organic molecules play an essential role in improving the electrochemical properties of the carbon nanomaterials. Based on the calculations, design principles are introduced to rationally design and predict the electrochemical properties of doped graphene and molecule-adsorbed graphene as metal-free catalysts for ORR and OER. An intrinsic descriptor is discovered for the first time, which can be used as a materials parameter for rational design of the metal-free catalysts with carbon nanomaterials for energy storage and conversion. The success of the design principle provides a better ...
Determinants of Corporate Governance Choices: Evidence from Listed Foreign Firms on U.S. Stock Exchanges
This study analyzes corporate governance practices of foreign (non-U.S.) issuers listed on the New York Stock Exchange (NYSE) and Nasdaq. Specifically, I examine the extent to which these foreign issuers voluntarily comply with U.S. stock exchange corporate governance requirements applicable to domestic issuers. My sample consists of 201 foreign companies primarily domiciled in Brazil, China, Israel, and the United Kingdom. I find that 151 (75 per cent) of the sample firms do not elect to comply with any of the U.S. corporate governance requirements. Logistic regression analysis generally supports the hypotheses that conformance with U.S. GAAP and percentage of managerial ownership are positively associated, and that percentage ownership by major shareholders is negatively associated with foreign firms electing to comply with U.S. corporate governance rules. This evidence is relevant for regulators and investors.
Determining Whether and When People Participate in the Events They Tweet About
This work describes an approach to determine whether people participate in the events they tweet about. Specifically, we determine whether people are participants in events with respect to the tweet timestamp. We target all events expressed by verbs in tweets, including past, present and events that may occur in future. We define event participant as people directly involved in an event regardless of whether they are the agent, recipient or play another role. We present an annotation effort, guidelines and quality analysis with 1,096 event mentions. We discuss the label distributions and event behavior in the annotated corpus. We also explain several features used and a standard supervised machine learning approach to automatically determine if and when the author is a participant of the event in the tweet. We discuss trends in the results obtained and devise important conclusions.
Development of an Outcome Measure for Use in Psychology Training Clinics
The ability to monitor client change in psychotherapy over time is vital to quality assurance in service delivery as well as the continuing improvement of psychotherapy research. Unfortunately, there is not currently a comprehensive, affordable, and easily utilized outcome measure for psychotherapy specifically normed and standardized for use in psychology training clinics. The current study took the first steps in creating such an outcome measure. Following development of an item bank, factor analysis and item-response theory analyses were applied to data gathered from a stratified sample of university (n = 101) and community (n = 261) participants. The factor structure did not support a phase model conceptualization, but did reveal a structure consistent with the theoretical framework of the research domain criteria (RDoC). Suggestions for next steps in the measure development process are provided and implications discussed.
Development of von Willebrand Factor Zebrafish Mutant Using CRISPR/Cas9 Mediated Genome Editing
von Willebrand factor (VWF) protein acts in the intrinsic coagulation pathway by stabilizing FVIII from proteolytic clearance and at the site of injury, by promoting the adhesion and aggregation of platelets to the exposed subendothelial wall. von Willebrand disease (VWD) results from quantitative and qualitative deficiencies in VWF protein. The variability expressivity in phenotype presentations is in partly caused by the action of modifier genes. Zebrafish has been used as hemostasis animal model. However, it has not been used to evaluate VWD. Here, we report the development of a heterozygote VWF mutant zebrafish using the genome editing CRISPR/Cas9 system to screen for modifier genes involved in VWD. We designed CRISPR oligonucleotides and inserted them into pT7-gRNa plasmid. We then prepared VWF gRNA along with the endonuclease Cas9 RNA from Cas9 plasmid. We injected these two RNAs into 1-4 cell-stage zebrafish embryos and induced a mutation in VWF exon 29 of the zebrafish with a mutagenesis rate of 16.6% (3/18 adult fish). Also, we observed a germline transmission with an efficiency rate of 5.5% (1/18 adult fish). We obtained a deletion in exon 29 which should result in truncated VWF protein.
Disease Tissue Imaging and Single Cell Analysis with Mass Spectrometry
Cells have been found to have an inherent heterogeneity that has led to an increase in the development of single-cell analysis methods to characterize the extent of heterogeneity that can be found in seemingly identical cells. With an understanding of normal cellular variability, the identification of disease induced cellular changes, known as biomarkers, may become more apparent and readily detectable. Biomarker discovery in single-cells is challenging and needs to focus on molecules that are abundant in cells. Lipids are widely abundant in cells and play active roles in cellular signaling, energy metabolism, and are the main component of cellular membranes. The regulation of lipid metabolism is often disrupted or lost during disease progression, especially in cancer, making them ideal candidates as biomarkers. Challenges exist in the analysis of lipids beyond those of single-cell analysis. Lipid extraction solvents must be compatible with the lipid or lipids of interest. Many lipids are isobaric making mass spectrometry analysis difficult without separations. Single-cell extractions using nanomanipulation coupled to mass spectrometry has shown to be an excellent method for lipid analysis of tissues and cell cultures. Extraction solvents are tunable for specific lipid classes, nanomanipulation prevents damage to neighboring cells, and lipid separations are possible through phase dispersion. The most important aspect of single-cell analysis is that it uncovers the extent of cellular heterogeneity that exists among cellular populations that remains undetected during averaged sampling.
"Don't Frack with Denton"
Don't Frack With Denton chronicles the ground-breaking movement to ban hydraulic fracturing in the city of Denton, Texas by combining observational location shooting with extensive sit-down interviews and carefully negotiated subject-filmmaker relationships to create a safe and comfortable space for thoughtful reflection and criticism of a complex social movement who's activities span several years and many individuals. The result is a long-form documentary that is unapologetically in solidarity with this movement's goals while simultaneously maintaining enough editorial independence and critical distance to allow the activists themselves to honestly evaluate their decision-making, tactics and interpersonal relationships in ways that will provide insight and instruction to similar movements around the country and the world.
EEG Signal Analysis in Decision Making
Decision making can be a complicated process involving perception of the present situation, past experience and knowledge necessary to foresee a better future. This cognitive process is one of the essential human ability that is required from everyday walk of life to making major life choices. Although it may seem ambiguous to translate such a primitive process into quantifiable science, the goal of this thesis is to break it down to signal processing and quantifying the thought process with prominence of EEG signal power variance. This paper will discuss the cognitive science, the signal processing of brain signals and how brain activity can be quantifiable through data analysis. An experiment is analyzed in this thesis to provide evidence that theta frequency band activity is associated with stress and stress is negatively correlated with concentration and problem solving, therefore hindering decision making skill. From the results of the experiment, it is seen that theta is negatively correlated to delta and beta frequency band activity, thus establishing the fact that stress affects internal focus while carrying out a task.