Studies on actomyosin crossbridge flexibility using a new single molecule assay.
Description:
Several key flexure sites exist in the muscle crossbridge including the actomyosin binding site which play important roles in the actomyosin crossbridge cycle. To distinguish between these sources of flexibility, a new single molecule assay was developed to observe the swiveling of rod about a single myosin. Myosins attached through a single crossbridge displayed mostly similar torsional characteristics compared to myosins attached through two crossbridges, which indicates that most of the tors…
more
Date:
May 2004
Creator:
Gundapaneni, Deepika