Mechanism of Activation by Autophosphorylation of an S6/H4 Kinase Isolated From Human Placenta
Description:
A novel molecular mechanism of autophosphorylation-dependent activation of the ser/thr S6/H4 kinase isolated from human placenta is described. Phosphopeptide mapping of the enzyme was used to determine the rate and extent of site-specific autophosphorylation. These data were correlated to phosphotransferase activity of the protein kinase. The results indicated that a sequential phosphorylation of two sites in the catalytic domain is required for maximum activation. Kinetic analysis determined t…
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Date:
December 1994
Creator:
Benner, Gretchen E. (Gretchen Evonne)