DNA Typing of HLA-B by PCR with Primer Mixes Utilizing Sequence-Specific Primers
Description:
The aim of this study was to design a resolution typing system for the HLA-B gene. This technique involves a one-step PCR reaction utilizing genomic DNA and sequence-specific primers to determine the specificity of each allele and to produce a larger primer data base ideal for serological analysis. The application of this technique to serological analysis can improve serology detection which is currently hindered by antibody cross-reactivity and the unavailability of useful typing reagents.
Date:
August 1997
Creator:
Chiu, Angela Chen-Yen