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Comparison and Genetic Analysis of Host Specificity in Cluster BD1 Bacteriophages infecting Streptomyces

Description: Bacteriophages are viruses that specifically infect bacteria. When a phage infects a bacterium, it attaches itself to the surface of the bacteria and injects its DNA into the intracellular space. The phage DNA hijacks the cellular machinery of the bacteria and forces it to produce phage proteins. Eventually, the bacteria cell bursts or lyses, releasing new phage. The bacteria act as a host for phage reproduction. The ability for a phage to infect multiple bacterial species is known as host rang… more
Date: May 2019
Creator: Klug, Hannah
Item Type: Refine your search to only Thesis or Dissertation
open access

Exploration of Genome Length, Burst Time, and Burst Size of Streptomyces griseus Bacteriophages

Description: Since phages use the host resources to replicate themselves after infection, the different sizes of the phage genome should influence the replication rate. We, therefore, hypothesized that the smaller genomes should burst the cell faster than the larger ones. As well, the shorter genomes would have greater burst sizes because they should replicate faster. Here, we obtained 16 phages of various genome length. All phages were isolated on Streptomyces griseus and available in our phage bank at the… more
Date: May 2019
Creator: Maneekul, Jindanuch
Item Type: Refine your search to only Thesis or Dissertation
open access

Isolation and Bioinformatic Characterization of Four Novel Bacteriophages from Streptomyces toxytricini

Description: Six initial phage isolates with high titer lysates were obtained using Streptomyces toxytricini B-5426 as the host bacterium. These isolates were named Goby, Toma, Yosif, Yara, Deema, and Hsoos. However, upon completion of the sequencing, it was found that the Yara and Hsoos isolates were identical, as were Goby and Deema. As a result, final analysis was completed on only the four unique isolates. All of the phages mentioned above were isolated from soil samples from different locations. Al… more
Date: May 2019
Creator: Alzaid, Hessah
Item Type: Refine your search to only Thesis or Dissertation
open access

Autonomic Nerve Activity and Cardiovascular Function in the Chicken Embryo (Gallus gallus)

Description: The goal of this study was to build on the historic use of the avian model of development and also to further the knowledge of autonomic nervous system (ANS) regulation of cardiovascular function in vertebrates. Vasoactive drugs sodium nitroprusside, a vasodilator and phenylephrine, a vasoconstrictor were used to study the correlation of cardiovascular function relationship with nerve activity, both sympathetic and parasympathetic (vagal). Additionally, ANG II was used to assess its effects on … more
Date: December 2018
Creator: Onyemaechi, Clinton
Item Type: Refine your search to only Thesis or Dissertation
open access

Producing a Film on Oil Spill Research for the Public

Description: The Deepwater Horizon oil drilling rig exploded on April 20, 2010, off the coast of Louisiana in the Gulf of Mexico. Following the spill, British Petroleum, leaser of the rig, set up a funding institution known as the Gulf of Mexico Research Initiative (GoMRI) to support research and understanding of the spill on the environments and peoples of the gulf. This outreach project was created alongside research of the RECOVER consortium, funded by GoMRI, to communicate what is happening within resea… more
Date: December 2018
Creator: Barnes, Emma Katherine
Item Type: Refine your search to only Thesis or Dissertation
open access

Analysis of Mature and Young Thrombocytes in Zebrafish

Description: Eukaryotic platelets are small cell fragments that are released into the bloodstream from megakaryocytes, and their production is initiated in the bone marrow. They are mainly involved in blood hemostasis and thrombus formation. The newly synthesized platelets are called reticulated platelets or young platelets. Zebrafish thrombocytes are equivalent to mammalian platelets and have similar characteristics and functions. Likewise, zebrafish has both young and mature thrombocytes. Only young throm… more
Date: August 2018
Creator: Fallatah, Weam
Item Type: Refine your search to only Thesis or Dissertation
open access

The Role of Thyroid Hormone on the Development of Endothermy in White Leghorn Chickens (Gallus gallus)

Description: As chickens hatch, there is a rapid change in their physiology and metabolism associated with attaining endothermy. It is thought that thyroid hormones (TH) play a major role in regulating developmental changes at hatching. In birds, TH regulates skeletal muscle growth, which has a direct impact on the chick's ability to thermoregulate via shivering thermogenesis. To better understand the role of TH in the timing of hatching, development of thermogenic capacity, and metabolic rate, we manipu… more
Date: August 2018
Creator: Rippamonti, Jessica D.
Item Type: Refine your search to only Thesis or Dissertation
open access

The Role of Transmembrane Protein 59 in Thrombocyte Function and the Effect of MS-222 on Hemostasis in Zebrafish

Description: Transmembrane protein 59 (tmem59) is a gene that encodes a protein involved in autophagy and apoptosis in human. A previous study in zebrafish showed that tmem59 mRNA was several folds higher in thrombocytes than those found in red blood cells (RBCs). Therefore, we hypothesized that tmem59 has a role in thrombocytes function. We injected a hybrid of control vivo-morpholino (cVMO) and tmem59 specific antisense standard oligonucleotide (tmem59SO) into adult zebrafish to knockdown tmem59.This pigg… more
Date: August 2018
Creator: Deebani, Afnan Omar M.
Item Type: Refine your search to only Thesis or Dissertation
open access

In Vitro Exploration of Functional Acrolein Toxicity with Cortical Neuronal Networks

Description: Acrolein is produced endogenously after traumatic brain injury (TBI) and is considered a primary mechanism for secondary damage occurring after TBI. We are using frontal cortex networks derived from mouse embryos and grown on microelectrode arrays in vitro to monitor the spontaneous activity of networks and the changes that occur after acrolein application. Networks exposed to acrolein exhibit a biphasic response profile. An initial increase in network activity, followed by a decrease to 100% a… more
Date: May 2018
Creator: Durant, Stormy R.
Item Type: Refine your search to only Thesis or Dissertation
open access

Isolation and Characterization of Phages Infecting Streptomyces azureus

Description: Isolating novel phages using Streptomyces azureus, which produces antibiotic thiostrepton, as a host, and characterizing the genomes may help us to find new tools that could be used to develop antibiotics in addition to contribute to the databases of phages and specifically, Streptomyces phages. Streptomyces phages Alsaber, Omar, Attoomi, Rowa, and ZamZam were isolated using during this study. They were isolated from enriched soil and sequenced by Illumina sequencing method. They were isolated … more
Date: May 2018
Creator: Sulaiman, Ahmad M.
Item Type: Refine your search to only Thesis or Dissertation
open access

Population Dynamics and Community Structure of Mosquitoes (Diptera: Culicidae) Recorded in Denton, Texas from 2005 to 2015

Description: A population survey was conducted on the mosquito species recorded in Denton, Texas for the years of 2005 to 2015. Data used in this project were obtained from an ongoing, long-term surveillance program led by the City of Denton and conducted through the University of North Texas. Research focused on the population dynamics and community structure of mosquitoes collected within urban areas of Denton, Texas in relation to certain environmental variables. A total of 80,837 female mosquitoes were … more
Date: May 2018
Creator: Hambrick, Bethany Lynn
Item Type: Refine your search to only Thesis or Dissertation
open access

Simultaneous Electrophysiological and Morphological Assessment of Impact Damage to Nerve Cell Networks

Description: A ballistic pendulum impulse generator was used to impact networks in primary culture growing on microelectrode arrays. This approach has the advantage of imparting pure tangential acceleration insults (50 to 300 g) with simultaneous morphological and electrophysiological multichannel monitoring for days before and after the impact. Action potential (AP) production, network activity patterns, and cell electrode coupling of individual units using AP waveshape templates were quantified. Network … more
Date: May 2018
Creator: Rogers, Edmond A.
Item Type: Refine your search to only Thesis or Dissertation
open access

Superbursts: Investigation of Abnormal Paroxysmal Bursting Activity in Nerve Cell Networks In Vitro

Description: Superbursts (SBs) are large, seemingly spontaneous activity fluctuations often encountered in high density neural networks in vitro. Little effort has been put forth to define and analyze SBs which are paroxysmal bursting discharges. Through qualitative and quantitative means, I have described specific occurrences of superbursting activity. A complex of paroxysmal bursting has been termed a "superburst episode," and each individual SB is a "superburst event" which is comprises a fine burst stru… more
Date: May 2018
Creator: Suri, Nikita
Item Type: Refine your search to only Thesis or Dissertation
open access

Corbicula fluminea Invasion as a Secondary Effect of Hydrilla verticillata Management via Triploid Grass Carp (Ctenopharyngodon idella)

Description: A study of Asian clam (Corbicula fluminea Müller) colonization in relation to changes in aquatic vegetation community as a result of management of Hydrilla verticillata (L. f.) Royle with grass carp was conducted at the Lewisville Aquatic Ecosystem Research Facility (LAERF), Lewisville, TX, from April 2015 through October 2016. Percent vegetation cover, C. fluminea abundance and water quality metrics (pH, turbidity, conductivity, DO, calcium, chlorophyll a) from 16 experimental subjects were an… more
Date: December 2017
Creator: Holbrook, David Lee
Item Type: Refine your search to only Thesis or Dissertation
open access

The Generation of Recombinant Zea mays Spastin and Katanin Proteins for In Vitro Analysis

Description: Plant microtubules play essential roles in cell processes such as cell division, cell elongation, and organelle organization. Microtubules are arranged in highly dynamic and ordered arrays, but unlike animal cells, plant cells lack centrosomes. Therefore, microtubule nucleation and organization are governed by microtubule-associated proteins, including a microtubule-severing protein, katanin. Mutant analysis and in vitro characterization has shown that the highly conserved katanin is needed fo… more
Date: December 2017
Creator: Alodailah, Sattam Sonitan
Item Type: Refine your search to only Thesis or Dissertation
open access

Homologs of Mammalian Lysosomal Lipase in Arabidopsis and Their Roles in Lipid Droplet Dynamics

Description: Lipid droplets (LDs) are organelles with many functions in cells and numerous protein interactors facilitate their biogenesis, maintenance, and turnover. The mammalian lipase responsible for LD turnover during lipophagy, LipA, has two candidate homologs in Arabidopsis: MPL1 and LIP1. One or both of these plant homologs may function in a similar manner to mammalian LipA, providing an LD breakdown pathway. To test this hypothesis, wild type (WT) Arabidopsis plants, MPL1 over-expressing (OE) muta… more
Date: December 2017
Creator: McClinchie, Elizabeth A
Item Type: Refine your search to only Thesis or Dissertation
open access

Phenotypic Analysis of Medicago truncatula NPF1.7 Over-Expressing Plants Grown under Different Nitrate Conditions

Description: Plants have many nitrate transporters; in the model legume Medicago truncatula, MtNPF1.7 is among them. MtNPF1.7 is important for M. truncatula growth and it has been established that MtNPF1.7 is a high affinity nitrate transporter. M. truncatula plants with mutations in MtNPF1.7 gene show defects during plants growth, with striking abnormalities in nodule development and root architecture. Nitrogen fixation is an energy expensive process; when legumes have sufficient bioavailable nitrogen like… more
Date: December 2017
Creator: Cai, Jingya
Item Type: Refine your search to only Thesis or Dissertation
open access

Delivery of CRISPR/Cas9 RNAs into Blood Cells of Zebrafish: Potential for Genome Editing in Somatic Cells

Description: Factor VIII is a clotting factor found on the intrinsic side of the coagulation cascade. A mutation in the factor VIII gene causes the disease Hemophilia A, for which there is no cure. The most common treatment is administration of recombinant factor VIII. However, this can cause an immune response that renders the treatment ineffective in certain hemophilia patients. For this reason a new treatment, or cure, needs to be developed. Gene editing is one solution to correcting the factor VIII muta… more
Date: August 2017
Creator: Schneider, Sara Jane
Item Type: Refine your search to only Thesis or Dissertation
open access

Development of a Targeted Protein Residue Analysis Approach in Archaeology

Description: Liquid chromatography-mass spectrometry (LC-MS) based proteomic methods have provided archaeologists with a powerful tool for the discovery and identification of proteins within artifacts. Traditionally, discovery-based methods have utilized a non-targeted full mass scan method in an attempt to identify all proteins present within a given sample. However, increased sensitivity is often needed to target specific proteins in order to test hypotheses. Proteins present within archaeological materia… more
Date: August 2017
Creator: Scott, Ashley
Item Type: Refine your search to only Thesis or Dissertation
open access

Evaluating the Role of UV Exposure and Recovery Regimes in PAH Photo-Induced Toxicity to Daphina Magna

Description: Polyaromatic hydrocarbons (PAHs) are contaminants synthesized through incomplete combustion of carbon based substances. PAHs are known to be photodynamic and toxicity increases exponentially when in contact with ultraviolet radiation (UV). The effect of UV absent recovery periods and potential for latent toxicity during photo-induced toxicity are previously unknown and are not included within the toxicity model. Results of equal interval tests further support the current reciprocity model as a … more
Date: August 2017
Creator: Gnau, Jennifer Leigh
Item Type: Refine your search to only Thesis or Dissertation
open access

Generating Molecular Biology Tools to Investigate the Ca2+ Binding Ability of Arabidopsis TON2

Description: The position of the cell division plane in plants is determined by the position of the preprophase band. The pre prophase band (PPB) is a ring of microtubules centered around the nucleus on the inner side of plasma membrane that establishes the cortical division site. The PPB forms at the end of G2 and breaks down at the end of prophase leaving behind protein markers of its position that are collectively called the cortical division site. During cytokinesis the phragmoplast expands towards the … more
Date: August 2017
Creator: Shao, Danyang
Item Type: Refine your search to only Thesis or Dissertation
open access

Investigating Human Gut Microbiome in Obesity with Machine Learning Methods

Description: Obesity is a common disease among all ages that has threatened human health and has become a global concern. Gut microbiota can affect human metabolism and thus may modulate obesity. Certain mixes of gut microbiota can protect the host to be healthy or predispose the host to obesity. Modern next-generation sequencing technique allows accessing huge amount of genetic information underlying microbiota and thus provides new insights into the functionality of these micro-organisms and their interac… more
Date: August 2017
Creator: Zhong, Yuqing
Item Type: Refine your search to only Thesis or Dissertation
open access

Neurological Responses to a Glucose Diet in Caenorhabditis elegans

Description: TRPV channels play a role in both mammalian insulin signaling, with TRPV1 expression in pancreatic beta-cells, and in C. elegans insulin-like signaling through expression of OSM-9, OCR-1, and OCR-2 in stress response pathways. In response to a glucose-supplemented diet, C. elegans are know to have sensitivity to anoxic stress, exhibit chemotaxis attraction, and display reduced egg-laying rate. Transcriptome analysis reveals that glucose stimulates nervous system activity with increased transcri… more
Date: August 2017
Creator: Dumesnil, Dennis
Item Type: Refine your search to only Thesis or Dissertation
open access

Adrenergic and Cholinergic Regulation of Cardiovascular Function in Embryonic Neotropic Cormorants (Phalacrocorax basilianus)

Description: Investigations of cholinergic and adrenergic tone on heart rate (fH) and mean arterial pressure (Pm) during embryonic development have been conducted on numerous avian species. While these investigations have documented that adrenergic tone, a continuous stimulation, on fH and Pm is vital to embryonic development in the birds studied to date, development of cholinergic tone on fH has been shown to vary even within species. Further, past studies have been bias to focus primarily on precocial spe… more
Date: May 2017
Creator: Cummins, James B.
Item Type: Refine your search to only Thesis or Dissertation
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