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open access

Assessing Regional Gully Erosion Risk: A Remote Sensing and Geographic Information Systems Approach

Description: Gully erosion has been established as a major source of sediment pollution in the upper Trinity River watershed in north-central Texas. This fact, along with a lack of models appropriate for a large-area gully erosion analysis established a need for a gully erosion study in the upper Trinity basin. This thesis project attempted to address this need by deriving an index indicative of gully erosion risk using remote sensing and geographic information systems (GIS) methodology. In context of previ… more
Date: August 1995
Creator: Sill, Paul E. (Paul Eric)
open access

Assessing the Effects of a Municipal Wastewater Treatment Plant Effluent on Zooplankton, Phytoplankton and Corbicula Flumina in a Constructed Wetland

Description: Wetland wastewater treatment offers low-cost, energy efficient alternatives to conventional wastewater technologies. In this study, an artificial wetland was constructed at the City of Denton, Texas Pecan Creek Water Reclamation Plant to facilitate diazinon removal from treated effluent.
Date: May 1995
Creator: Hymel, Stephanie Ramick
open access

An Assessment of Storm Water Toxicity from the Dallas/Fort Worth Metroplex and Denton, Texas

Description: With the advent of national storm water regulations, municipalities with populations greater than 100,000 are required to obtain National Pollutant Discharge Elimination System Permits (NPDES) for storm water discharges. In addition to the sampling required for the permit process, the City of Fort Worth contracted with the University of North Texas' Institute of Applied Sciences to conduct acute toxicity testing using Pimephales prcmelas and Ceriodaphnia dubia on storm water samples received fr… more
Date: August 1995
Creator: Keating, Paul Redmond
open access

Cell-Free Recovery and Isotopic Identification of Cyanide Degrading Enzymes from Pseudomonas Fluorescens

Description: Cell-free extracts from Pseudomonas fluorescens NCIMB 11764 catalyzed the degradation of cyanide into products that included C02, formic acid, formamide and ammonia. Cyanide-degrading activity was localized to cytosolic cell fractions and was observed at substrate concentrations as high as 100 mM. Two cyanide degrading activities were identified by: (i) the determination of reaction products stoichiometries, (ii) requirements for NADH and oxygen, and (iii) kinetic analysis. The first activity p… more
Date: December 1995
Creator: Wang, Chien-Sao
open access

Effects of External Electric Fields on Light Transmittance in Isolated Crayfish Nerves

Description: Acute effects of a pulsed external electric field (PEEF) at 20 V/cm and a d.c. EEF at 90 V/cm on light transmittance in an isolated compound crayfish nerve was measured. In a third series, the nerve was pre-treated with the Na+ channel blocker tetrodotoxin (TTX). A PEEF produced an irreversible increase in the variation of light transmittance in normal nerves but a reversible increase in TTX treated nerves. This data was statistically insignificant. The d.c. EEFs produced a reversible and stati… more
Date: December 1995
Creator: Northcutt, Brian S. W.
open access

An Evaluation of Fish and Macroinvertebrate Response to Effluent Dechlorination in Pecan Creek

Description: This study evaluated the effects of chlorinated effluent discharged from the City of Denton, Texas' wastewater treatment plant on Pecan Creek's fish and macroinvertebrate assemblages, and their recovery upon dechlorination. A baseline of ecological conditions was established while chlorine was present in the effluent (June 1993- October 1993), and was evaluated again after dechlorination with sulfur dioxide (October 1993-August 1994). In situ Asiatic clam and fathead minnow ambient toxicity tes… more
Date: May 1995
Creator: Wise, Patricia D. (Patricia Diane)
open access

GABAᴀ Receptors in Rat Whisker Barrel Cortex: Effects of Sensory Deprivation

Description: The GABAergic system in adult sensory cortex is affected by sensory deprivation, but little is known about how this predominant inhibitory system is affected during ontogeny. The present study investigates developmental effects of whisker trimming on GABAa receptors in rat barrel cortex. Rats trimmed for 6 wk beginning at birth and adulthood showed similar decreases in [3H]muscimol binding in deprived relative to non-deprived barrels, suggesting absence of a critical period.
Date: August 1995
Creator: Salazar, Eduardo, 1962-
open access

Heat Shock Proteins in Ascaris suum

Description: Ascaris suum were exposed to a number of stressors, including heavy metals and both high (40°C) and low (18°C) temperatures. The 70kD and 90kD heat shock proteins (HSPs) in the different A. suum tissues were analyzed by Western blot and quantitated by Macintosh Image Program.
Date: August 1995
Creator: Chao, Sheng-Hao
open access

In Vitro Studies of Nuclear Changes in Mammalian CNS Neurons Subjected to Rapid Acceleration Impact Injury

Description: An in vitro model of Rapid Acceleration Impact (RAI) Injury was used to study the effects of multiple impact (220 g/impact, 3-5 sec intervals) trauma on cultures of murine CNS cells. Investigations with spinal cord cultures showed that 1) multiple impacts delivered tangential to the plane of cell growth caused neuronal death (12% after 3 impacts to 46% after 10 impacts); 2) multiple impacts delivered normal to the plane of cell growth were much less effective (8% dead after 10 impacts); 3) most… more
Date: May 1995
Creator: Wolf, Amy
open access

Pyrimidine Salvage Enzymes in Microorganisms: Labyrinths of Enzymatic Diversity

Description: Pyrimidine salvage pathways are essential to all cells. They provide a balance of RNA synthesis with the biosynthetic pathway in pyrimidine prototrophs and supply all the pyrimidine requirements in auxotrophs. While the pyrimidine biosynthetic pathway is found in almost all organisms and is nearly identical throughout nature, the salvage pathway often differs from species to species, with aspects of salvage seen in every organism. Thus significant taxonomic value may be ascribed to the salvage … more
Date: December 1995
Creator: Beck, Debrah A. (Debrah Ann)
open access

Quantitative Analysis of the Gabaergic System in Cat Primary Somatosensory Cortex and Its Relation to Receptive Field Properties

Description: Sensory neocortex contains a significant number of inhibitory neurons that use gamma-aminobutyric acid (GABA) as their neurotransmitter. Functional roles for these neurons have been identified in physiological studies. For example, in primary somatosensory cortex (SI), blockade of GABAa receptors with bicuculline leads to expansion of receptive fields (RFs). The magnitude of RF enlargement varies between SIpopulations of GABAergic neurons were identified by labeling specific calcium binding pro… more
Date: May 1995
Creator: Li, Jianying
open access

Regulation of Escherichia coli pyrBI Gene Expression in Pseudomonas fluorescens

Description: Pseudomonas fluorescens does not appear to regulate the enzymes of de novo pyrimidine biosynthesis at the level of gene expression. Little or no apparent repression of pyr gene expression is observed upon addition of exogenous pyrimidines to the growth medium. The Escherichia coli pyrBI genes for aspartate transcarbamoylase (ATCase) were sized down and cloned into the broad host range plasmid, pKT230. Upon introduction into a P.fluorescenspyrB mutant strain, ATCase showed repression in response… more
Date: May 1995
Creator: Shen, Weiping
open access

Site Directed Mutagenesis of Dienelactone Hydrolase

Description: The clcD gene encoding dienelactone hydrolase (DLH) is part of the clc gene cluster for the utilization of the B-ketoadipate pathway intermediate chlorocatechol. The roles that individual amino acids residues play in catalysis and binding of the enzyme were investigated. Using PCR a 1.9 kbp clcD fragment was amplified and subcloned yielding a 821 bp BamHi to ZscoRI subclone in the plasmid pUC19.
Date: August 1995
Creator: Al-Khatib, Haifa Yousef
open access

Subcellular Localization of N-acylphosphatidyl-ethanolamine Synthase in Cotyledons of Cotton Seedlings

Description: N-acylation of phosphatidylethanolamine (PE) with free fatty acids catalyzed by N-acyl phosphatidylethanolamine (NAPE) synthase was reported in cotyledons of 24-h-old cotton seedlings. Here I report subcellular localization of this enzyme. Differential centrifugation, sucrose density gradient fractionation,aqueous two-phase partitioning and electron microscopy techniques were utilized to elucidate subcellular site(s) of NAPE synthase. Marker enzymes were used to locate organelles in subcellular… more
Date: December 1995
Creator: Sriparameswaran, Anuja
open access

A Two Semester Life Science Syllabus for Use in Texas Public Schools with Seventh Grade Students

Description: The problem of using a state adopted textbook written to apply to a large body of students with varying interests and needs was overcome by using a detailed syllabus that arranged course content in a meaningful sequence that appealed to student interest. The outlined syllabus prepared a two semester life science curriculum to be used by the teacher to guide lesson planning. Both semesters were divided into three units each. Materials included in the syllabus were given to actual student groups … more
Date: May 1995
Creator: Edwards, Gail G. (Gail Graham)
open access

Upper and Lower Temperature Tolerances of Three Freshwater Game-Fish Species Exposed to Cycling Temperatures

Description: A total of 670 critical thermal maxima (CTMax) and minima (CTMin) were determined for three freshwater fishes acclimated to three constant temperatures and a diel regimen cycling between the lowest and highest acclimation temperatures. In all species temperature tolerance was directly related to acclimation temperature and slopes relating these variables indicate that acclimation temperature has a greater influence on tolerance of low rather than high temperatures. CTMax and CTMin values genera… more
Date: August 1995
Creator: Currie, Rebecca J. (Rebecca Jean)
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