Description: Culture health of Ceriodaphnia dubia was evaluated for organisms cultured using all combinations of three foods and three waters. Criteria used to assess health of cultures included adult and neonate weights, time required to produce first broods, neonate production, adult survival, and resistance to hexavalent chromium. Diet/water combinations which produced the most neonates were not found to produce adults which were more resistant to chromium than those which produced fewer neonates. Of those evaluated, a diet of Selenastrum capricornutum and a yeast-trout chow-cereal leaf mixture was best for culturing and testing Ceriodaphnia. The best synthetic water tested was a mixture of nine parts reconstituted hard water and one part bottled mineral water.
Date: May 1990
Creator: Patterson, Paul W. (Paul William)