Stress Response by Alternative σ-factor, RpoH, and Analysis of Posttranslational Modification of the Heat Shock Protein, Dnak, in Escherichia coli
Description:
Bacteria have developed specialized responses that involve the expression of particular genes present in a given regulon. Sigma factors provide regulatory mechanisms to respond to stress by acting as transcriptional initiation factors. This work focuses on σ32 during oxidative stress in Escherichia coli. The differential response of key heat shock (HS) genes was investigated during HS and oxidative stress using qPCR techniques. While groEL and dnaJ experienced increases in transcriptional respo…
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Date:
May 2015
Creator:
Martinez, Sarah N.