The purpose of this project is to produce a complete and thorough notebook that may be used to supplement laboratory coursework. Its intent is to be used primarily by the students to aid them in understanding background information and the proper laboratory procedures involved in various types of experiments. The laboratory notebook is a summation of all the experiments and procedures used in the six-credit hour Advanced Microbial and Molecular Biology (BIOL 5160) course offered during the summer semester at the University of North Texas. This class is a team taught effort by Professors O'Donovan and Kunz. The course is constructed as an intensive practice exercise to teach the student about gene mutations, biosynthetic pathways, preparation and analysis of plasmid DNA, and many other topics included in the notebook.
This study used a geographic information system (GIS) and an empirical pollutant load model to estimate average annual pollutant loads contained in storm water charges from the City of Carrollton, Texas. The Geographic Resource Analyses Support System (GRASS) was used to create a number of spatial data layers as input variables to a model that estimates loads in Kilograms/Hectare/Year, and Kilogram/Basin/Year. Average annual pollutant loads for twelve pollutants were derived from average annual rainfall/runoff volumes, coupled with Local Dallas/Fort Worth Metroplex storm water Event Mean Concentrations.
Since the introduction of management practices by the Forest Service to stabilize red-cockaded woodpecker (RCW) populations, the number of cavity trees killed by southern pine beetles (SPB) has increased. A model of the landscape ecology of RCW and SPB in the Homochitto National Forest was created using data collected from the Forest Service and Global Atmospherics. The conclusions of the study were that the RCW and SPB utilize the same type of habitat and the stand hazard maps are an accurate means of determining the locations of SPB infestations. The functional heterogeneity maps created for the SPB and RCW would be useful predictors of future occurrences of either species if complete data were obtained.
In recent years there has been a great deal of scientific interest in processes that affect the fate of organic chemicals in the environment. one main reason for this increased interest is due to greater environmental concern over accidental or purposeful release of these chemicals into the environment by man. A major environmental concern is the increased use of pesticides over the last few years. In the thirty years prior to 1978 the use of pesticides has increased by a factor of forty (Ridgeway et al., 1978). Recently the use of herbicides has been increasing, but that of insecticides has stabilized (Willis, 1983). Detectable amounts of organic pesticides can be found in many areas of the biosphere. For toxic organic chemicals to be used safely, researchers must have a clear understanding of the fate and persistence of these chemicals when they are released into the environment. This understanding will also allow the development of new products that, when properly used, will not produce adverse effects to man or the environment (Weber, 1972). According to the Toxic Substance Control Act (TSCA) any new or expanded-use chemical that might be released into the environment must be tested for environmental hazard.
Wastewater effluent produced in the fiberglass manufacturing industry contains a significant amount of total suspended solids. Environmental regulations require pretreatment of effluent before it is discharged to the municipal wastewater treatment plant. Chemical precipitation by coagulation and flocculation is the method of pretreatment used at the Vetrotex CertainTeed Corporation (VCT). A treatability study was conducted to determine conditions at which the VCT Wastewater Pretreatment Plant could operate to consistently achieve a total suspended solids concentration ≤ 200-mg/L. Jar tests varied pH, polymer dosage, and ferric sulfate dosage. Total suspended solids and turbidity were measured to evaluate treatment performance. The data were used to determine an optimum set of conditions under project guidelines. Of twelve polymers screened, BPL 594 was selected as the most effective polymer. For cost efficiency in the wastewater pretreatment operation, recommendations suggested that treatment chemical injection be electronically controlled according to turbidity of the treated effluent.
Simultaneous size fractionation of plankton populations associated with NaH^14CO_3 and ^3H-glucose uptake was employed in eutrophic Lake Texoma (Texas and Oklahoma) and oligotrophic Flathead Lake (Montana). Autoradiography was utilized to determine the role of specific microorganisms in community metabolism. Ultraplankton (0.45-10 μm) dominated plankton numbers and metabolic activity in both aquatic systems. Many of the most abundant species were not the most productive, in terms of inorganic C fixation. Rates of heterotrophic uptake of ^3H-glucose were small in comparison to photolithotrophic uptake in both lakes, Photoheterotrophy was more extensive in Flathead Lake, Autoradiographs indicated that bacteria were responsible for observed photoheterotrophy. Oscillatoria sp. exhibited. mixotrophy in Lake Texoma,
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