UNT Theses and Dissertations - 2 Matching Results

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A New LC Column for the Separation and the Quantitation of Nucleotides

A new column, Dionex AS4A, (polystyrenedivinylbenzene matrix) used for the separation of ribonucleotides and deoxyribonucleotides for the first time, and previously used for ion analysis was found superior to conventional silica columns because it separates ribonucleotides and deoxyribonucleotides. Resolution of dGTP was not possible with the Dionex column and CTP and GDP often co-eluted. Using conventional silica columns, monophosphates separated from diphosphates and diphosphates from triphos… more

Date: December 1987

Creator: Brock, Patricia C. (Patricia Charlene)

Item Type: Thesis or Dissertation

Partner: UNT Libraries

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Physical Characterization and Restriction Mapping of the Sal Plasmid From Pseudomonas Putida

Physical and restriction mapping of the salicylate catabolic plasmid SAL from Pseudomonas putida strain PpG 2119 was carried out by standard multiple restriction analysis and by cross hybridization studies using radioactively labeled restriction fragment probes. The total numbers of fragments produced, their respective sizes, the arrangement of the restriction fragments on the plasmid and the map locations of the enzyme recognition sites for Hpal, Xhol, Dral and Smal are given.