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Nutritional Requirements of Corynebacterium poinsettiae

Description: In a minimal medium supplemented with glucose and yeast extract, the optimum pH for the growth of C. poinsettias was found to be 7.5. The organism requires thiamine, biotin, and pantothenic acid for growth. No absolute requirement was found for any amino acid, purine or pyrimidine although an amino acid mixture was stimulatory. Casamino acids could be substituted for the synthetic amino acid mixture. Yeast extract provided an additional factor(s) necessary for maximal growth. The results sugges… more
Date: December 1983
Creator: Hooshdaran, Farideh
Partner: UNT Libraries
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Plasma Ion Concentrations in Selected Fishes from Four North Central Texas Reservoirs with Different Salinities

Description: Mean salinity concentrations in the four reservoirs (Moss, Ray Hubbard, Texoma and Possum Kingdom) ranged from 0.2 ppt in Moss Lake to 2.01 ppt in Possum Kingdom Lake. Reservoir sodium and chloride concentrations were hypotonic to hypertonic to plasma levels in all species. Interspecific differences were seen in ionic concentrations within each reservoir. Total osmotic and sodium concentrations in carp, Cyprinus carpio, were correlated to their concentrations in the reservoirs. No such relation… more
Date: December 1983
Creator: Del Regno, Kenneth J. (Kenneth Joseph)
Partner: UNT Libraries
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Properties of Normal Rat Kidney Cells Transformed by a Temperature-Sensitive Mutant (LA31) of Rous Sarcoma Virus

Description: The basis of this investigation is to characterize growth property differences in normal versus virally transformed cells. Using a temperature-sensitive mutant of Rous sarcoma virus, the cells' transformation state is regulated by the growth temperature; at 33°C the cells are transformed, while at 39°C the cells have normal characteristics. The morphology of NRK cells is elongated and fibroblastic; when transformed the cells are rounded. Normal cells grow to a monolayer and stop, while transfor… more
Date: August 1983
Creator: Connolly, John R. (John Robert)
Partner: UNT Libraries
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Studies on the Purification and Phosphorylation of Phosphofructokinase from Ascaris suum

Description: A new procedure has been developed to concentrate the phosphofructokinase from muscle of Ascaris suum with minimum loss of activity. By utilizing this method, 50 ml fraction was concentrated to a final volume of 3 ml in about 1.5 h without loss in enzyme activity. The concentrated enzyme had a specific activity of 64 units per mg. Ascaris muscle-cuticle was incubated in 50 1M solutions of either acetylcholine, serotonin, y-aminobutyric acid, levamisole, or saline alone. Phosphate analysis of th… more
Date: August 1983
Creator: Kaeini, Mohammad R. (Mohammad Reza)
Partner: UNT Libraries
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Effects of N⁶,O²'-Dibutyryl Cyclic Adenosine 3' ,5' Monophosphate on Transformation of Rat Kidney Cells and Chick Embryo Fibroblasts by Wild-Type and Temperature-Sensitive Rous Sarcoma Virus

Description: N^6,O^2' -Dibutyryl cyclic adenosine 3',5'-monophosphate (Bt_2cAMP) was investigated for its effects on various tissue culture cells infected with temperature-sensitive (ts) mutant, LA31 and Bratislava 77 (B77), a wild-type Rous sarcoma virus. Specifically, known parameters of transformation were investigated and a possible site of action has been tenably proposed. The drug Bt_2cAMP was found to have little effect on the transformation related properties of primary chick embryo fibroblasts (CEF… more
Date: December 1983
Creator: Marshall, David A. (David Allen)
Partner: UNT Libraries
open access

Failure of Azotobacter Vinelandii to Fix Nitrogen in Soil

Description: It was found that Azotobacter vinelandii grown in a dialyzed soil medium did not fix nitrogen and had a much lower rate of respiration than when grown in Burk's nitrogen-free medium. When para-hydroxybenzoic acid served as the added oxidizable organic carbon source in dialyzed soil medium, the azotobacter grown in it were found to be unable to fix nitrogen. On the other hand, A. vinelandii fixed nitrogen when grown in soil supplemented with glucose. It was concluded that natural conditions in t… more
Date: August 1983
Creator: Taeed-Kashani, Taraneh
Partner: UNT Libraries
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