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Modulation of ³H-Myo-Inositol Uptake by Glucose and Sorbitol in Cultured Bovine Lens Epithelial Cells

Description: Myo-[3H]-inositol accumulation in cultured bovine lens epithelial cells (BLECs) occurred by both high- and low affinity, Nat-dependent transport sites. High ambient glucose significantly inhibited myo-[ 3 H]-inositol uptake; the co-administration of sorbinil, an aldose reductase inhibitor, prevented the inhibitory effect on the low affinity transport site. A glucose-sensitive process for myo-[3 H]-inositol uptake on the high-affinity transport site was uncovered by Lineweaver-Burk analysis. Dixon plot analysis confirmed that the effect of glucose was due to competitive inhibition of the high-affinity myo-inositol transport site while the effect of sorbitol was due to competitive inhibition of the low-affinity myo-inositol transport site.
Date: August 1992
Creator: Chen, Hai-Qing
Partner: UNT Libraries