Tailoring seed oil composition in the real world: optimising omega-3 long chain polyunsaturated fatty acid accumulation in transgenic Camelina sativa

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This article discusses the further tailoring of the seed oil profile to reduce the omega-6 content, and an evaluation of the performance of such GM plants under field conditions in terms of agronomic performance and the lipidomic profile of seed oil.

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12 p.

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Usher, Sarah; Han, Lihua; Haslam, Richard P.; Michaelson, Louise V.; Sturtevant, Drew; Aziz, Mina et al. July 26, 2017.

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This article discusses the further tailoring of the seed oil profile to reduce the omega-6 content, and an evaluation of the performance of such GM plants under field conditions in terms of agronomic performance and the lipidomic profile of seed oil.

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12 p.

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Abstract: There is considerable interest in the de novo production of omega-3 long chain polyunsaturated fatty acids such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), not least of all given the importance of these fatty acids in both aquaculture and human nutrition. Previously we have demonstrated the feasibility of using metabolic engineering in transgenic plants (Camelina sativa) to modify the seed oil composition to now include EPA and/or DHA. In this study, we further tailored the seed oil profile to reduce the omega-6 content, and evaluated the performance of such GM plants under field conditions (i.e. environmental releases), in terms of agronomic performance and also the lipidomic profile of seed oil. We used MALDI- mass spectrometry imaging to identify discrete tissue-types in the seed in which these non-native fatty acids preferentially accumulated. Collectively, these data provide new insights into the complexity of plant lipid metabolism and the challenges associated with predictive manipulation of these pathways. However, this study identified the likely dispensable nature of a Δ12-desturase activity in our omega-3 metabolic engineering rationales for Camelina.

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  • Scientific Reports, 2017. London, UK: Nature Publishing Group

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  • Publication Title: Scientific Reports
  • Volume: 7
  • Pages: 1-12
  • Peer Reviewed: Yes

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  • July 26, 2017

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  • Aug. 29, 2017, 9:38 a.m.

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Usher, Sarah; Han, Lihua; Haslam, Richard P.; Michaelson, Louise V.; Sturtevant, Drew; Aziz, Mina et al. Tailoring seed oil composition in the real world: optimising omega-3 long chain polyunsaturated fatty acid accumulation in transgenic Camelina sativa, article, July 26, 2017; London, United Kingdom. (digital.library.unt.edu/ark:/67531/metadc991054/: accessed December 13, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT College of Arts and Sciences.